Chromogenic media for ESBL-positive Enterobacteriaceae

ChromID ESBL agar and Brilliance ESBL agar were compared for the isolation of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae from 298 stools. These had comparable sensitivity and selectivity for the 116 positive samples. Pre-enrichment with cefpodoxime and extending incubation to 48 hours after direct plating both significantly increased sensitivity but reduced selectivity of both agars.This work was supported by a grant awarded to SP from the UK Department of Health, Wellcome Trust and the Health Innovation Challenge Fund (HICF-T5-342 and WT098600). TG is a Wellcome Trust Research Training Fellow. MET is a Clinician Scientist Fellow, supported by the Academy of Medical Sciences and the Health Foundation. The authors declare no conflicts of interest.This is the final version of the article. It first appeared from Elsevier via http://dx.doi.org/10.1016/j.diagmicrobio.2015.11.00

Comparison of two chromogenic media for the detection of vancomycin-resistant enterococcal carriage by nursing home residents.

We compared ChromID VRE and Brilliance VRE media for the detection of vancomycin-resistant enterococci (VRE). Using a panel of 28 enterococcal isolates, 10 vanA Enterococcus faecium and three vanA Enterococcus faecalis isolates grew as per manufacturers' instructions whilst growth of two vanC and eight vancomycin-susceptible enterococci was inhibited on both media. Important differences were noted in the selectivity and chromogenic properties of the two media for vanA Enterococcus raffinosus and vanB E. faecium. The two media were further evaluated using 295 stool samples from nursing home residents, 34 of which grew VRE (11.5%). ChromID and Brilliance had comparable sensitivity, which was increased markedly by prolonging incubation to 48 hours (from 29% to 82%, and from 41% to 85%, respectively) and by a pre-enrichment step (to 97% and 100%, respectively). Brilliance VRE agar had higher selectivity at 48 hours, and after pre-enrichment.This publication presents independent research supported by the Health Innovation Challenge Fund (WT098600, HICF-T5-342), a parallel funding partnership between the Department of Health and Wellcome Trust. The views expressed in this publication are those of the author(s) and not necessarily those of the Department of Health or Wellcome Trust. TG is a Wellcome Trust Research Training Fellow. MET is a Clinician Scientist Fellow supported by the Academy of Medical Sciences and the Health Foundation. SJP and MET are supported by the NIHR Cambridge Biomedical Research Centre.This is the final version of the article It first appeared from Elsevier via https://dx.doi.org/10.1016/j.diagmicrobio.2016.04.02

The systematic identification of content and delivery style of an exercise intervention

Objective: This study explored the utility of using behaviour change taxonomies and checklists to systematically assess the content and delivery of behavioural support for physical activity delivered through an established exercise-referral scheme. Design: An observation study was conducted whereby 22% of initial consultations were observed and audio-recorded, using quota sampling stratified by exercise-referral advisor.Main Outcome Measures: Content was independently coded by two researchers, to assess; i) completeness in delivering the programme protocol, ii) behaviour change techniques delivered (defined using the CALO-RE taxonomy), and iii) delivery style according to the Behaviour Change Counselling Index (BECCI).Results: Protocol completeness was 63.6% (range 35.6%-74.6%). The behaviour change techniques delivered most consistently were ‘providing information about where and when to perform the behavior' (86%) and ‘setting outcome goals’ (82%). Other evidence-based techniques such as self-monitoring were infrequently observed. Variation in BECCI scores indicated that advisors could, but did not consistently, provide a client-centred service. Conclusion: This study highlights how theoretically informed taxonomies can be useful in evaluating service delivery within applied practice, providing a meaningful way of assessing the completeness of protocol delivery relative to evidence. The provision of feedback to practitioners based on such objective criteria also facilitated positive academic-practitioner communication.<br/

Examining predictors of school belonging using a socio-ecological perspective

Between the years of 2003–2015, the Programme for International Student Assessment (PISA) has identified a global trend signalling a decline in a sense of school belonging for secondary school students. Research has identified several factors that are positively related to school belonging, such as teacher support and academic motivation. However, little empirical research has been conducted to evaluate the relevant school belonging variables holistically and to assess their socio-ecological levels (e.g., student, microsystem, mesosystem) relative to the student. The purpose of this study is to assess the significant predictive variables within each socio-ecological level regarding school belonging. For this purpose, this study used data collected by PISA in 2015, focusing on data from 309,785 15-year-old students attending 12,668 schools in 52 countries around the world. Hierarchical multiple regression analysis was conducted to a) examine the empirical support for a layered structure of sense of school belonging, b) explore the contributions of variables in each layer of the socio-ecosystem to explain the variability in sense of school belonging and c) examine potential variations in this ability across schools and countries. The models provided support for the existence of such layers but also for some underlying relationships across the variables in the layers of the socio-ecosystem. The study then concludes with a discussion of the implications of the findings for school leaders, teachers and parents with respect to how school belonging approaches and strategies can be absorbed into existing practices and operations at school

The Detection of an Extremely Bright Fast Radio Burst in a Phased Array Feed Survey

We report the detection of an ultra-bright fast radio burst FRB)from a modest, 3.4-day pilot survey with the Australian Square Kilometre Array Pathfinder. The survey was conducted in a wide- field fly’s-eye configuration using the phased-array-feed technology deployed on the array to instantaneously observe an effective area of 160 deg2, and achieve an exposure totalling 13200 deg2 hr. We constrain the position of FRB 170107 to a region 8 ́ x 8 ́ in size(90% containment)and its fluence to be 58 ± 6 Jy ms. The spectrum of the burst shows a sharp cutoff above 1400 MHz, which could be due to either scintillation or an intrinsic feature of the burst. This confirms the existence of an ultra-bright (> 20 Jy ms) population of FRBs

Incorporating kinetic effects on Nernst advection in inertial fusion simulations

We present a simple method to incorporate nonlocal effects on the Nernst advection of magnetic fields down steep temperature gradients, and demonstrate its effectiveness in a number of inertial fusion scenarios. This is based on assuming that the relationship between the Nernst velocity and the heat flow velocity is unaffected by nonlocality. The validity of this assumption is confirmed over a wide range of plasma conditions by comparing Vlasov-Fokker-Planck and flux-limited classical transport simulations. Additionally, we observe that the Righi-Leduc heat flow is more severely affected by nonlocality due to its dependence on high velocity moments of the electron distribution function, but are unable to suggest a reliable method of accounting for this in fluid simulations

Estimating aboveground carbon density and its uncertainty in Borneo's structurally complex tropical forests using airborne laser scanning

Borneo contains some of the world's most biodiverse and carbon-dense tropical forest, but this 750 000 km(2) island has lost 62% of its old-growth forests within the last 40 years. Efforts to protect and restore the remaining forests of Borneo hinge on recognizing the ecosystem services they provide, including their ability to store and sequester carbon. Airborne laser scanning (ALS) is a remote sensing technology that allows forest structural properties to be captured in great detail across vast geographic areas. In recent years ALS has been integrated into statewide assessments of forest carbon in Neotropical and African regions, but not yet in Asia. For this to happen new regional models need to be developed for estimating carbon stocks from ALS in tropical Asia, as the forests of this region are structurally and composition-ally distinct from those found elsewhere in the tropics. By combining ALS imagery with data from 173 permanent forest plots spanning the lowland rainforests of Sabah on the island of Borneo, we develop a simple yet general model for estimating forest carbon stocks using ALS-derived canopy height and canopy cover as input metrics. An advanced feature of this new model is the propagation of uncertainty in both ALS- and ground-based data, allowing uncertainty in hectare-scale estimates of carbon stocks to be quantified robustly. We show that the model effectively captures variation in aboveground carbon stocks across extreme disturbance gradients spanning tall dipterocarp forests and heavily logged regions and clearly outperforms existing ALS-based models calibrated for the tropics, as well as currently available satellite-derived products. Our model provides a simple, generalized and effective approach for mapping forest carbon stocks in Borneo and underpins ongoing efforts to safeguard and facilitate the restoration of its unique tropical forests.Peer reviewe