A fáscia transversal e o colágeno na hérnia inguinal direta

OBJECTIVE: The main objective os the present work is to identify the constitutive elements of 23 samples of tranversalis fascia from direct inguinal hernia patients compairing to 22 fascia from corpses.MATERIAL AND METHODS: The samples were obtained at the moment of videolaparospic repair. They were stained for haematoxiline-eosine and immunohistochemistry with collagen type I monoclonal antibody.RESULTS: The dense connective tissue was the principal constitutive element in both groups, although less present in the patient one, followed by adipose tissue. We were able to identify, by immunohistochemistry, the type I collagen as the main protein.CONCLUSION: The authors conclude that transversalis fascia is mainly formed by type I collagen and suggest to use hernia repair technics that increase the collagen deposition in fascia, like when using mesh.OBJETIVO: O objetivo do presente estudo é o de identificar os elementos histológicos constituintes da fáscia transversal em 23 pacientes com hérnia inguinal direta, comparando-os com a fáscia de 22 cadáveres sem esta doença.MATERIAIS E MÉTODOS: As biópsias dos pacientes foram obtidas no momento da correção videolaparoscópica. As fáscias foram estudadas histologicamente, sendo empregadas as técnicas de coloração de hematoxilina-eosina e anticorpo monoclonal para colágeno tipo I.RESULTADOS: O tecido conjuntivo denso foi o principal elemento constitutivo das fáscias de pacientes e controles, embora em menor presença nos primeiros, seguido do tecido adiposo. Identificamos, através da técnica de imuno-histoquímica, o colágeno tipo I como sendo a principal proteína das fáscias.CONCLUSÃO: Os autores concluem que a fáscia transversal é constituída principalmente por colágeno tipo I e sugerem o uso de técnicas de correção de hérnia inguinal que aumentem a deposição de colágeno na fáscia, como quando do uso de próteses

Transarterial Embolization and Percutaneous Ethanol Injection as an Effective Bridge Therapy before Liver Transplantation for Hepatitis C-Related Hepatocellular Carcinoma

Background. Transarterial chemoembolization alone or in association with radiofrequency ablation is an effective bridging strategy for patients with hepatocellular carcinoma awaiting for a liver transplant. However, cost of this therapy may limit its utilization. This study was designed to evaluate the outcomes of a protocol involving transarterial embolization, percutaneous ethanol injection, or both methods for bridging hepatocellular carcinomas prior to liver transplantation. Methods. Retrospective review of all consecutive adult patients who underwent a first liver transplant as a treatment to hepatitis C-related hepatocellular carcinoma at our institution between 2002 and 2012. Primary endpoint was patient survival. Secondary endpoint was complete tumor necrosis. Results. Forty patients were analyzed, age 58 ± 7 years. There were 23 males (57.5%). Thirty-six (90%) out of the total 40 patients were within Milan criteria. Complete necrosis was achieved in 19 patients (47.5%). One-, 3-, and 5-year patient survival were, respectively, 87.5%, 75%, and 69.4%. Univariate analysis did not reveal any variable to impact on overall patient survival. Conclusions. Transarterial embolization, ethanol injection, or the association of both methods followed by liver transplantation comprises effective treatment strategy for hepatitis C-related hepatocellular carcinoma. This strategy should be adopted whenever transarterial chemoembolization and/or radiofrequency ablation are not available options

The sub-basin springs of Poxim river, state of Sergipe: the degradation to restoration

Estudos ambientais em bacias hidrográficas são fundamentais para o entendimento do uso dos recursos naturais e dos problemas relacionados à ocupação do espaço. Em Sergipe, há necessidade de se obter informações para fins de planejamento e gestão futura de suas bacias hidrográficas, uma vez que gradualmente observa-se uma redução do volume produzido nos cursos d'água e da qualidade de suas águas, que são imprescindíveis para o abastecimento humano, a utilização na agricultura e na indústria. Devido ao acelerado processo de supressão da vegetação na sub-bacia hidrográfica do rio Poxim, formada pelos rios Poxim-mirim, Poxim-açu, e Pitanga e seus pequenos tributários, foi realizado um diagnóstico para se verificar o estado de conservação das suas principais nascentes, a situação quanto ao fluxo de água, as formas de uso e ocupação do solo no entorno destas e os tipos fisionômicos de vegetação remanescentes. As informações obtidas serão úteis para a realização de projetos de restauração ambiental, a promoção de melhorias no ambiente e nas comunidades rurais e resgate da diversidade da flora e fauna nestas áreas. Observou-se que as 20 principais nascentes dos rios e tributários que compõem a sub-bacia hidrográfica do rio Poxim, apresentam alterações decorrentes da acelerada antropização (90%), a maioria delas (65%) com elevada degradação (sem raio mínimo de 50m de vegetação) e ocupadas por agricultura (50%) e pastagens (35%). Somente duas nascentes encontram-se preservadas. Quanto à composição florística, as espécies identificadas (43) podem ser utilizadas em projetos futuros para restauração das nascentes e dos cursos d'água nesta sub-bacia hidrográfica. _________________________________________________________________________________________ ABSTRACT: Environmental studies on river basins are fundamental to understanding the natural resources use and the problems related to the space occupation. In Sergipe, it is necessary to obtain information for planning purposes and future management of their watersheds, once the watercourses volume and its quality are reducing gradually, which are essential for human supply and use in agriculture and industry. Due to the accelerated removal of vegetation in the river Poxim sub-basin, formed by the Poxim-Mirim, Poxim-Açu, Pitanga rivers, and its small tributaries, a diagnosis was carried out to verify the conservation status of their main sources, the situation on the water flow, the different uses, and land use around these sources and the remaining vegetation physiognomic types. The information is useful for carrying out environmental restoration projects, promoting improvements in the environment and in the rural communities and for the flora and fauna diversity rescue in these areas. It was observed that the 20 rivers sources top and tributaries that compose the river Poxim sub-basin showed changes from accelerated human anthropization (90%), most (65%) with high degradation (no vegetation in the minimum radius of 50 m), being occupied by agriculture (50%) and pastures (35%). Only two sources were preserved. The floristic composition, the identified species (43) can be used in future projects for sources and watercourses restoration in this sub-basin

Characterization of Brazilian oils by FTIR and SUVF spectroscopy. A comparison with GC/MS results

La aplicación de IRTF y FUVS en varios petróleos de Brasil ha contribuido al establecimiento de parámetros complementarios que permiten evidenciar diferencias entre ellos. Estas diferencias están ligadas al ambiente de deposición de la roca madre (lacustre de agua dulce a salobre) como revelan los parámetros moleculares de CG/EM. También se han destacado las diferencias relacionadas con la biodegradación