Development of the SPECULOOS exoplanet search project

SPECULOOS (Search for habitable Planets EClipsing ULtra-cOOl Stars) aims to perform a transit search on the nearest ($<40$pc) ultracool ($<3000$K) dwarf stars. The project's main motivation is to discover potentially habitable planets well-suited for detailed atmospheric characterisation with upcoming giant telescopes, like the James Webb Space Telescope (JWST) and European Large Telescope (ELT). The project is based on a network of 1m robotic telescopes, namely the four ones of the SPECULOOS-Southern Observatory (SSO) in Cerro Paranal, Chile, one telescope of the SPECULOOS-Northern Observatory (SNO) in Tenerife, and the SAINT-Ex telescope in San Pedro M\'artir, Mexico. The prototype survey of the SPECULOOS project on the 60~cm TRAPPIST telescope (Chile) discovered the TRAPPIST-1 system, composed of seven temperate Earth-sized planets orbiting a nearby (12~pc) Jupiter-sized star. In this paper, we review the current status of SPECULOOS, its first results, the plans for its development, and its connection to the Transiting Exoplanet Survey Satellite (TESS) and JWST

Fancine 2003, n. 08

Abstract not availabl

SPECULOOS: a network of robotic telescopes to hunt for terrestrial planets around the nearest ultracool dwarfs

We present here SPECULOOS, a new exoplanet transit search based on a network of 1m-class robotic telescopes targeting the $\sim$1200 ultracool (spectral type M7 and later) dwarfs bright enough in the infrared ($K$-mag $\leq 12.5$) to possibly enable the atmospheric characterization of temperate terrestrial planets with next-generation facilities like the $\textit{James Webb Space Telescope}$. The ultimate goals of the project are to reveal the frequency of temperate terrestrial planets around the lowest-mass stars and brown dwarfs, to probe the diversity of their bulk compositions, atmospheres and surface conditions, and to assess their potential habitability.Comment: 21 pages, 13 figures, 1 table. Proceedings of SPI

Super-resolution imaging as a method to study GPCR dimers and higher-order oligomers

The study of G protein-coupled receptor (GPCR) dimers and higher-order oligomers has unveiled mechanisms for receptors to diversify signaling and potentially uncover novel therapeutic targets. The functional and clinical significance of these receptor–receptor associations has been facilitated by the development of techniques and protocols, enabling researchers to unpick their function from the molecular interfaces, to demonstrating functional significance in vivo, in both health and disease. Here we describe our methodology to study GPCR oligomerization at the single-molecule level via super-resolution imaging. Specifically, we have employed photoactivated localization microscopy, with photoactivatable dyes (PD-PALM) to visualize the spatial organization of these complexes to <10 nm resolution, and the quantitation of GPCR monomer, dimer, and oligomer in both homomeric and heteromeric forms. We provide guidelines on optimal sample preparation, imaging parameters, and necessary controls for resolving and quantifying single-molecule data. Finally, we discuss advantages and limitations of this imaging technique and its potential future applications to the study of GPCR function

Discovery of WASP-174b : Doppler tomography of a near-grazing transit

We report the discovery and tomographic detection of WASP-174b, a planet with a near-grazing transit on a 4.23-d orbit around a V= 11.9, F6V star with [Fe/H] = 0.09 ± 0.09. The planet is in a moderately misaligned orbit with a sky-projected spin–orbit angle of λ = 31° ± 1°. This is in agreement with the known tendency for orbits around hotter stars to be misaligned. Owing to the grazing transit, the planet’s radius is uncertain with a possible range of 0.8–1.8 RJup. The planet’s mass has an upper limit of 1.3 MJup. WASP-174 is the faintest hot-Jupiter system so far confirmed by tomographic means.Publisher PDFPeer reviewe

Circadian-Related Heteromerization of Adrenergic and Dopamine D4 Receptors Modulates Melatonin Synthesis and Release in the Pineal Gland

Dopamine and adrenergic receptor complexes form under a circadian-regulated cycle and directly modulate melatonin synthesis and release from the pineal gland

Circadian-Related Heteromerization of Adrenergic and Dopamine D4 Receptors Modulates Melatonin Synthesis and Release in the Pineal Gland

Dopamine and adrenergic receptor complexes form under a circadian-regulated cycle and directly modulate melatonin synthesis and release from the pineal gland

TOI-2084 b and TOI-4184 b: two new sub-Neptunes around M dwarf stars

We present the discovery and validation of two TESS exoplanets orbiting nearby M dwarfs: TOI-2084b, and TOI-4184b. We characterized the host stars by combining spectra from Shane/Kast and Magellan/FIRE, SED (Spectral Energy Distribution) analysis, and stellar evolutionary models. In addition, we used Gemini-South/Zorro & -North/Alopeke high-resolution imaging, archival science images, and statistical validation packages to support the planetary interpretation. We performed a global analysis of multi-colour photometric data from TESS and ground-based facilities in order to derive the stellar and planetary physical parameters for each system. We find that TOI-2084b and TOI-4184b are sub-Neptune-sized planets with radii of Rp = 2.47 +/- 0.13R_Earth and Rp = 2.43 +/- 0.21R_Earth, respectively. TOI-2084b completes an orbit around its host star every 6.08 days, has an equilibrium temperature of T_eq = 527 +/- 8K and an irradiation of S_p = 12.8 +/- 0.8 S_Earth. Its host star is a dwarf of spectral M2.0 +/- 0.5 at a distance of 114pc with an effective temperature of T_eff = 3550 +/- 50 K, and has a wide, co-moving M8 companion at a projected separation of 1400 au. TOI-4184b orbits around an M5.0 +/- 0.5 type dwarf star (Kmag = 11.87) each 4.9 days, and has an equilibrium temperature of T_eq = 412 +/- 8 K and an irradiation of S_p = 4.8 +/- 0.4 S_Earth. TOI-4184 is a metal poor star ([Fe/H] = -0.27 +/- 0.09 dex) at a distance of 69 pc with an effective temperature of T_eff = 3225 +/- 75 K. Both planets are located at the edge of the sub-Jovian desert in the radius-period plane. The combination of the small size and the large infrared brightness of their host stars make these new planets promising targets for future atmospheric exploration with JWST.Comment: Accepted for publication in A&

CCR2 Acts as Scavenger for CCL2 during Monocyte Chemotaxis

<div><h3>Background</h3><p>Leukocyte migration is essential for effective host defense against invading pathogens and during immune homeostasis. A hallmark of the regulation of this process is the presentation of chemokines in gradients stimulating leukocyte chemotaxis via cognate chemokine receptors. For efficient migration, receptor responsiveness must be maintained whilst the cells crawl on cell surfaces or on matrices along the attracting gradient towards increasing concentrations of agonist. On the other hand agonist-induced desensitization and internalization is a general paradigm for chemokine receptors which is inconsistent with the prolonged migratory capacity.</p> <h3>Methodology/Principal Findings</h3><p>Chemotaxis of monocytes was monitored in response to fluorescent CCL2-mCherry by time-lapse video microscopy. Uptake of the fluorescent agonist was used as indirect measure to follow the endogenous receptor CCR2 expressed on primary human monocytes. During chemotaxis CCL2-mCherry becomes endocytosed as cargo of CCR2, however, the internalization of CCR2 is not accompanied by reduced responsiveness of the cells due to desensitization.</p> <h3>Conclusions/Significance</h3><p>During chemotaxis CCR2 expressed on monocytes internalizes with the bound chemoattractant, but cycles rapidly back to the plasma membrane to maintain high responsiveness. Moreover, following relocation of the source of attractant, monocytes can rapidly reverse their polarization axis organizing a new leading edge along the newly formed gradient, suggesting a uniform distribution of highly receptive CCR2 on the plasma membrane. The present observations further indicate that during chemotaxis CCR2 acts as scavenger consuming the chemokine forming the attracting cue.</p> </div

CXCR4 Mediated Chemotaxis Is Regulated by 5T4 Oncofetal Glycoprotein in Mouse Embryonic Cells

5T4 oncofetal molecules are highly expressed during development and upregulated in cancer while showing only low levels in some adult tissues. Upregulation of 5T4 expression is a marker of loss of pluripotency in the early differentiation of embryonic stem (ES) cells and forms an integrated component of an epithelial-mesenchymal transition, a process important during embryonic development and metastatic spread of epithelial tumors. Investigation of the transcriptional changes in early ES differentiation showed upregulation of CXCL12 and down-regulation of a cell surface protease, CD26, which cleaves this chemokine. CXCL12 binds to the widely expressed CXCR4 and regulates key aspects of development, stem cell motility and tumour metastasis to tissues with high levels of CXCL12. We show that the 5T4 glycoprotein is required for optimal functional cell surface expression of the chemokine receptor CXCR4 and CXCL12 mediated chemotaxis in differentiating murine embryonic stem cells and embryo fibroblasts (MEF). Cell surface expression of 5T4 and CXCR4 molecules is co-localized in differentiating ES cells and MEF. By contrast, differentiating ES and MEF derived from 5T4 knockout (KO) mice show only intracellular CXCR4 expression but infection with adenovirus encoding mouse 5T4 restores CXCL12 chemotaxis and surface co-localization with 5T4 molecules. A series of chimeric constructs with interchanged domains of 5T4 and the glycoprotein CD44 were used to map the 5T4 sequences relevant for CXCR4 membrane expression and function in 5T4KO MEF. These data identified the 5T4 transmembrane domain as sufficient and necessary to enable CXCR4 cell surface expression and chemotaxis. Furthermore, some monoclonal antibodies against m5T4 can inhibit CXCL12 chemotaxis of differentiating ES cells and MEF which is not mediated by simple antigenic modulation. Collectively, these data support a molecular interaction of 5T4 and CXCR4 occurring at the cell surface which directly facilitates the biological response to CXCL12. The regulation of CXCR4 surface expression by 5T4 molecules is a novel means to control responses to the chemokine CXCL12 for example during embryogenesis but can also be selected to advantage the spread of a 5T4 positive tumor from its primary site