The Application of Advanced Composites for the Construction of Commercial Transport Aircraft

This individual Capstone project examined and evaluated current industry methods of testing, certification, and maintenance of advanced composite materials for the construction of commercial transport aircraft and the FAA regulations governing their use. The project critically compared and contrasted existing FAA standards and regulations governing the testing, certification, and maintenance of advanced composites for commercial transport aircraft structural applications with current industry practices to determine whether there were any areas of conflict between the two in order to accept or reject that current testing, certification, and maintenance procedures for advanced composites used in primary and secondary commercial transport aircraft structures are standardized throughout the aerospace industry and sufficiently capable of detecting damage or component failure. This was accomplished by performing a qualitative and quantitative analysis utilizing meta-analysis to contrast and compare past and current aerospace composite materials studies with non-destructive inspection (NDI) testing and structural health monitoring (SHM) data to determine statistical significance that supported or refuted the hypothesis of comprehensive process improvement throughout the industry. The results of the analysis showed that the hypothesis was accepted for testing and certification, but overwhelmingly rejected for current maintenance and repair. In addition, industry concerns were examined to determine whether limitations exist that would preclude the future use of advanced composites in structural applications based on current FAA standards and regulations. This project determined how current industry practices and FAA methodologies for the testing, certification, and maintenance of advanced composites in commercial transport aircraft structural applications may need to be modified in order to capture and address future industry use

Optical/Near-Infrared Observations of GRO J1744-28

We present results from a series of optical (g and r-band) and near-infrared (K'-band) observations of the region of the sky including the entire XTE and ROSAT error circles for the ``Bursting Pulsar'' GRO J1744-28. These data were taken with the Astrophysical Research Consortium's 3.5-m telescope at Apache Point Observatory and with the 2.2-m telescope at the European Southern Observatory. We see no new object, nor any significant brightening of any known object, in these error circles, with the exception of an object detected in our 8 February 1996 image. This object has already been proposed as a near-infrared counterpart to GRO J1744-28. While it is seen in only two of our ten 8 February frames, there is no evidence that this is an instrumental artifact, suggesting the possibility of near-infrared flares from GRO J1744-28, similar to those that have been reported from the Rapid Burster. The distance to the ``Bursting Pulsar'' must be more than 2 kpc, and we suggest that it is more than 7 kpc.Comment: 21 pages, 5 JPEG plates, 2 postscript figures. This paper will appear in the May 1, 1997 edition of the Astrophysical Journa

Characterization of a broad-based mosquito yeast interfering RNA larvicide with a conserved target site in mosquito semaphorin-1a genes

BACKGROUND: RNA interference (RNAi), which has facilitated functional characterization of mosquito neural development genes such as the axon guidance regulator semaphorin-1a (sema1a), could one day be applied as a new means of vector control. Saccharomyces cerevisiae (baker's yeast) may represent an effective interfering RNA expression system that could be used directly for delivery of RNA pesticides to mosquito larvae. Here we describe characterization of a yeast larvicide developed through bioengineering of S. cerevisiae to express a short hairpin RNA (shRNA) targeting a conserved site in mosquito sema1a genes. RESULTS: Experiments conducted on Aedes aegypti larvae demonstrated that the yeast larvicide effectively silences sema1a expression, generates severe neural defects, and induces high levels of larval mortality in laboratory, simulated-field, and semi-field experiments. The larvicide was also found to induce high levels of Aedes albopictus, Anopheles gambiae and Culex quinquefasciatus mortality. CONCLUSIONS: The results of these studies indicate that use of yeast interfering RNA larvicides targeting mosquito sema1a genes may represent a new biorational tool for mosquito control

Influence of Urban Landscapes on Population Dynamics in a Short-Distance Migrant Mosquito: Evidence for the Dengue Vector Aedes aegypti

Worldwide, 2.5 billion people are at risk for dengue infection, with no vaccine or treatment available. Thus dengue prevention is largely focused on controlling its mosquito vector, Aedes aegypti. Traditional mosquito control approaches typically include insecticide applications and breeding site source reduction. Presently, novel dengue control measures including the sterile insect technique and population replacement with dengue-incompetent transgenic mosquitoes are also being considered. Success of all population control programs is in part dependent upon understanding mosquito population ecology, including how anthropogenic effects on the urban landscape influence dispersal and expansion. We conducted a two year population genetic study examining how a major metropolitan highway impacts mosquito dispersal in Trinidad, West Indies. As evidenced by significant differentiation using both nuclear and mitochondrial DNA sequences, the highway acted as a significant barrier to dispersal. Our results suggest that anthropogenic landscape features can be used effectively to enhance population suppression/replacement measures by defining mosquito control zones along recognized landscape barriers that limit population dispersal

Enhancing Genome Investigations in the Mosquito Culex quinquefasciatus via BAC Library Construction and Characterization

Background Culex quinquefasciatus (Say) is a major species in the Culex pipiens complex and an important vector for several human pathogens including West Nile virus and parasitic filarial nematodes causing lymphatic filariasis. It is common throughout tropical and subtropical regions and is among the most geographically widespread mosquito species. Although the complete genome sequence is now available, additional genomic tools are needed to improve the sequence assembly. Findings We constructed a bacterial artificial chromosome (BAC) library using the pIndigoBAC536 vector and HindIII partially digested DNA isolated from Cx. quinquefasciatus pupae, Johannesburg strain (NDJ). Insert size was estimated by NotI digestion and pulsed-field gel electrophoresis of 82 randomly selected clones. To estimate genome coverage, each 384-well plate was pooled for screening with 29 simple sequence repeat (SSR) and five gene markers. The NDJ library consists of 55,296 clones arrayed in 144 384-well microplates. Fragment insert size ranged from 50 to 190 kb in length (mean = 106 kb). Based on a mean insert size of 106 kb and a genome size of 579 Mbp, the BAC library provides ~10.1-fold coverage of the Cx. quinquefasciatus genome. PCR screening of BAC DNA plate pools for SSR loci from the genetic linkage map and for four genes associated with reproductive diapause in Culex pipiens resulted in a mean of 9.0 positive plate pools per locus. Conclusion The NDJ library represents an excellent resource for genome assembly enhancement and characterization in Culex pipiens complex mosquitoes

Population Health Metrics During the Early Stages of the COVID-19 Pandemic: Correlative Pilot Study

Background: COVID-19 has caused nearly 1 million deaths in the United States, not to mention job losses, business and school closures, stay-at-home orders, and mask mandates. Many people have suffered increased anxiety and depression since the pandemic began. Not only have mental health symptoms become more prevalent, but alcohol consumption has also increased during this time. Helplines offer important insight into both physical and mental wellness of a population by offering immediate, anonymous, cheap, and accessible resources for health and substance use disorders (SUD) that was unobstructed by many of the mandates of the pandemic. Further, the pandemic also launched the use of wastewater surveillance, which has the potential for tracking not only population infections but also consumption of substances such as alcohol. Objective: This study assessed the feasibility of using multiple public surveillance metrics, such as helpline calls, COVID-19 cases, and alcohol metabolites in wastewater, to better understand the need for interventions or public health programs in the time of a public health emergency. Methods: Ethanol metabolites were analyzed from wastewater collected twice weekly from September 29 to December 4, 2020, in a Midwestern state. Calls made to the helpline regarding housing, health care, and mental health/SUD were correlated with ethanol metabolites analyzed from wastewater samples, as well as the number of COVID-19 cases during the sampling period. Results: Correlations were observed between COVID-19 cases and helpline calls regarding housing and health care needs. No correlation was observed between the number of COVID-19 cases and mental health/SUD calls. COVID-19 cases on Tuesdays were correlated with the alcohol metabolite ethyl glucuronide (EtG). Finally, EtG levels were negatively associated with mental health/SUD helpline calls. Conclusions: Although helpline calls provided critical services for health care and housing-related concerns early in the pandemic, evidence suggests helpline calls for mental health/SUD-related concerns were unrelated to COVID-19 metrics. Instead, COVID metrics were associated with alcohol metabolites in wastewater. Although this research was formative, with continued and expanded monitoring of population metrics, such as helpline usage, COVID-19 metrics, and wastewater, strategies can be implemented to create precision programs to address the needs of the population

Measuring Perceived Research Competence of Junior Researchers

Graduates of doctoral (Ph.D.) programs are expected to be competent at designing and conducting research independently. Given the level of research competence needed to successfully conduct research, it is important that assessors of doctoral programs (e.g., faculty and staff) have a reliable and validated tool for measuring and tracking perceived research competence among their students and graduates. A high level of research competence is expected for all Ph.D. graduates worldwide, in addition to in all disciplines/fields. Moreover, graduates of Ph.D. programs may complete their studies in one country but then obtain a research position in another country, emphasizing the need to ensure that all doctoral programs are fostering similar levels of research competence. Thus, the purpose of this study was to gather additional evidence for validity and reliability of the Research Competence (R-Comp) scale. Specifically, we sought to extend the findings of by adapting the scale, translating it to other languages, and applying the tool with a sample of early stage researchers. Our findings provide initial evidence that the adapted PR-Comp is appropriate for use in three languages and across a variety of disciplines/programs of study