Whole-blood transcriptomic signatures induced during immunization by chloroquine prophylaxis and Plasmodium falciparum sporozoites

A highly effective vaccine that confers sterile protection to malaria is urgently needed. Immunization under chemoprophylaxis with sporozoites (CPS) consistently confers high levels of protection in the Controlled Human Malaria infection (CHMI) model. To provide a broad, unbiased assessment of the composition and kinetics of direct ex vivo human immune responses to CPS, we profiled whole-blood transcriptomes by RNA-seq before and during CPS immunization and following CHMI challenge. Differential expression of genes enriched in modules related to T cells, NK cells, protein synthesis, and mitochondrial processes were detected in fully protected individuals four weeks after the first immunization. Non-protected individuals demonstrated transcriptomic changes after the third immunization and the day of treatment, with upregulation of interferon and innate inflammatory genes and downregulation of B-cell signatures. Protected individuals demonstrated more significant interactions between blood transcription modules compared to non-protected individuals several weeks after the second and third immunizations. These data provide insight into the molecular and cellular basis of CPS-induced immune protection from P. falciparum infection

Applied Remote Sensing Program (ARSP)

There are no author-identified significant results in this report

Experimental cross sections of Ho 165 (α,n) Tm 168 and Er 166 (α,n) Yb 169 for optical potential studies relevant for the astrophysical γ process

Background: Optical potentials are crucial ingredients for the prediction of nuclear reaction rates needed in simulations of the astrophysical γ process. Associated uncertainties are particularly large for reactions involving α particles. This includes (γ,α) reactions which are of special importance in the γ process. Purpose: The measurement of (α,n) reactions allows for an optimization of currently used α-nucleus potentials. The reactions Ho165(α,n) and Er166(α,n) probe the optical model in a mass region where γ process calculations exhibit an underproduction of p nuclei which is not yet understood. Method: To investigate the energy-dependent cross sections of the reactions Ho165(α,n) and Er166(α,n) close to the reaction threshold, self-supporting metallic foils were irradiated with α particles using the FN tandem Van de Graaff accelerator at the University of Notre Dame. The induced activity was determined afterwards by monitoring the specific β-decay channels. Results: Hauser-Feshbach predictions with a widely used global α potential describe the data well at energies where the cross sections are almost exclusively sensitive to the α widths. Increasing discrepancies appear towards the reaction threshold at lower energy. Conclusions: The tested global α potential is suitable at energies above 14 MeV, while a modification seems necessary close to the reaction threshold. Since the γ and neutron widths show non-negligible impact on the predictions, complementary data are required to judge whether or not the discrepancies found can be solely assigned to the α width. © 2014 American Physical Society.Peer reviewedFinal Accepted Versio

Critical temperature and the transition from quantum to classical order parameter fluctuations in the three-dimensional Heisenberg antiferromagnet

We present results of extensive quantum Monte Carlo simulations of the three-dimensional (3D) S=1/2 Heisenberg antiferromagnet. Finite-size scaling of the spin stiffness and the sublattice magnetization gives the critical temperature Tc/J = 0.946 +/- 0.001. The critical behavior is consistent with the classical 3D Heisenberg universality class, as expected. We discuss the general nature of the transition from quantum mechanical to classical (thermal) order parameter fluctuations at a continuous Tc > 0 phase transition.Comment: 5 pages, Revtex, 4 PostScript figures include