Transcriptomic analysis of insecticide resistance in the lymphatic filariasis vector Culex quinquefasciatus

Culex quinquefasciatus plays an important role in transmission of vector-borne diseases of public health importance, including lymphatic filariasis (LF), as well as many arboviral diseases. Currently, efforts to tackle C. quinquefasciatus vectored diseases are based on either mass drug administration (MDA) for LF, or insecticide-based interventions. Widespread and intensive insecticide usage has resulted in increased resistance in mosquito vectors, including C. quinquefasciatus. Herein, the transcriptome profile of Ugandan bendiocarb-resistant C. quinquefasciatus was explored to identify candidate genes associated with insecticide resistance. High levels of insecticide resistance were observed for five out of six insecticides tested, with the lowest mortality (0.97%) reported to permethrin, while for DDT, lambdacyhalothrin, bendiocarb and deltamethrin the mortality rate ranged from 1.63–3.29%. Resistance to bendiocarb in exposed mosquitoes was marked, with 2.04% mortality following 1 h exposure and 58.02% after 4 h. Genotyping of the G119S Ace-1 target site mutation detected a highly significant association (p 8-fold increase vs unexposed controls). These results provide evidence that bendiocarb resistance in Ugandan C. quinquefasciatus is mediated by both target-site mechanisms and over-expression of detoxification enzymes

Spatial consistency for multiple source direction-of-arrival estimation and source counting.

A conventional approach to wideband multi-source (MS) direction-of-arrival (DOA) estimation is to perform single source (SS) DOA estimation in time-frequency (TF) bins for which a SS assumption is valid. The typical SS-validity confidence metrics analyse the validity of the SS assumption over a fixed-size TF region local to the TF bin. The performance of such methods degrades as the number of simultaneously active sources increases due to the associated decrease in the size of the TF regions where the SS assumption is valid. A SS-validity confidence metric is proposed that exploits a dynamic MS assumption over relatively larger TF regions. The proposed metric first clusters the initial DOA estimates (one per TF bin) and then uses the members' spatial consistency as well as its cluster's spread to weight each TF bin. Distance-based and density-based clustering are employed as two alternative approaches for clustering DOAs. A noise-robust density-based clustering is also used in an evolutionary framework to propose a method for source counting and source direction estimation. The evaluation results based on simulations and also with real recordings show that the proposed weighting strategy significantly improves the accuracy of source counting and MS DOA estimation compared to the state-of-the-art

Maternal probiotic Lactocaseibacillus rhamnosus HN001 treatment alters postpartum anxiety, cortical monoamines and gut microbiome

International audiencePeripartum mood and anxiety disorders (PMADs) affect 15–20% of peripartum women and are well known to disrupt infant caregiving. A recent study in humans reported that anxiety and depressive symptoms were alleviated by peripartum treatment with the probiotic, Lactocaseibacillus rhamnosus HN001. The current study determined the effects of chronic Lactocaseibacillus rhamnosus HN001 (HN001) treatment on postpartum affective and caregiving behaviors in a laboratory rodent model. Female rats were given probiotic overnight in their drinking water, or untreated water, from the first day of pregnancy through postpartum day 10. To determine whether the HN001 effects were influenced by a background of stress, half the females underwent chronic variable pregnancy stress and the other half remained undisturbed. The results revealed that, even without pregnancy stress, HN001 reduced postpartum anxiety-related behavior, increased variability in behavioral fragmentation when dams interacted with pups, increased time away from pups, and decreased prefrontal cortex norepinephrine (NE), dopamine (DA) and serotonin (5-HT). Probiotic plus stress consistently reduced the latency to float in the forced swim test, increased DA and 5-HT turnovers in the prefrontal cortex, increased hippocampal NE, and reduced hypothalamic DA. Fecal microbe alpha and beta diversities were lower postpartum than prepartum, which was prevented by the probiotic treatment and/or stress. Across the entire sample lower postpartum anxiety behavior was associated with lower fecal Bacteroides dorei. This study reveals novel information about how L. rhamnosus HN001 influences postpartum behavior and microbiota-gut-brain physiology in female laboratory rats, with implications for probiotic supplement use by pregnant and postpartum women

Maternal probiotic Lactocaseibacillus rhamnosus HN001 treatment alters postpartum anxiety, cortical monoamines, and the gut microbiome

Peripartum mood and anxiety disorders (PMADs) affect 15–20% of peripartum women and are well known to disrupt infant caregiving. A recent study in humans reported that anxiety and depressive symptoms were alleviated by peripartum treatment with the probiotic, Lactocaseibacillus rhamnosus HN001. The current study determined the effects of chronic Lactocaseibacillus rhamnosus HN001 (HN001) treatment on postpartum affective and caregiving behaviors in a laboratory rodent model. Female rats were given probiotic overnight in their drinking water, or untreated water, from the first day of pregnancy through postpartum day 10. To determine whether the HN001 effects were influenced by a background of stress, half the females underwent chronic variable pregnancy stress and the other half remained undisturbed. The results revealed that, even without pregnancy stress, HN001 reduced postpartum anxiety-related behavior, increased variability in behavioral fragmentation when dams interacted with pups, increased time away from pups, and decreased prefrontal cortex norepinephrine (NE), dopamine (DA) and serotonin (5-HT). Probiotic plus stress consistently reduced the latency to float in the forced swim test, increased DA and 5-HT turnovers in the prefrontal cortex, increased hippocampal NE, and reduced hypothalamic DA. Fecal microbe alpha and beta diversities were lower postpartum than prepartum, which was prevented by the probiotic treatment and/or stress. Across the entire sample lower postpartum anxiety behavior was associated with lower fecal Bacteroides dorei. This study reveals novel information about how L. rhamnosus HN001 influences postpartum behavior and microbiota-gut-brain physiology in female laboratory rats, with implications for probiotic supplement use by pregnant and postpartum women. © 2024 Elsevier Lt

CORRECTION OF DEVIATIONS OF PSYCHO-PHYSIOLOGICAL STATUS OF HAZARDOUS OCCUPATIONS SPECIALISTS WITH USE OF HYPOXIC GAS-AIR ENVIRONMENTS

The study included 26 hazardous occupations specialists (men aged 20-32 years old), with signs of abnormalities of the psycho-physiological status (PPS) involved in intensive work. In the basic group (BG) (n=14) the specialists were trained with use of hypoxic artificial gas-air environments -HAGAE (O 2 content 15-17 %, duration of each procedure 45 min, the total number of procedures 12). For 12 people (the comparison group - CG), HAGAE conditions were simulated. PPS deviations were evaluated according to the state of the sensorimotor properties, the electroencephalographic (EEG) criteria, the level of reactive anxiety (RA). The studies in both groups were conducted prior to the correction measures (simulation) application, directly after the end of the program and in a month. In all individuals of the BG after the workouts, there was recorded an improvement of the PPS parameters compared to the initial level, such changes were absent in the CG, for which reason, there have been found statistically significant (p &lt; 0.05) intergroup differences in a number of the sensorimotor parameters, the EEG criteria, the RA level. The program based on the use of HAGAE, can be regarded as a method of choice in restoring PPS of hazardous occupations specialists.</jats:p