173 research outputs found
Validation of the CFD code Flow-3D for the free surface flow around the shipsâ hulls
This paper describes the Computational Fluid Dynamics (CFD) calculations that were completed to model the free surface flow around the shipsâ hulls. Published experimental data for the DTRC 5415 combatant model is commonly used for validation of numerical codes.
Simulations were performed using the software Flow-3D, a Reynoldâs Averaged Navier-Stokes (RANS) solver with structured orthogonal mesh. The verification was based on the examination of the flow around the hull for range of speeds and by comparison of the results for resistance obtained by CFD simulations and by experiments. Additional
analysis has been conducted to investigate mesh sensitivity and the implementation of different advection schemes. The second order advection scheme with monotonicity preserving was optimal for the qualitative analysis of the problem under consideration.
This study shows that CFD code Flow-3D has a limited capability to resolve the physics of the flow around the hull. The shape of the free surface and wave distributions around the hull corresponds approximately to the experimental observations.
For quantitative analysis of ship total resistance, Flow-3D shows a lack of accuracy. It appears that the code does not have the capability to properly resolve boundary layer on the hull and properly predict frictional resistance. It can be improved by using only dynamic pressure results and by using some established empirical/experimental approach
for estimating frictional resistance.
The multi-block grids and the different turbulent
models are being used to obtain valid numerical results that are crucial for making sound design decision
Neural Networks and Particle Swarm Optimization for Function Approximation in Tri-SWACH Hull Design
Tri-SWACH is a novel multihull ship design that is well suited to a wide range of industrial, commercial, and military applications, but which because of its novelty has few experimental studies on which to base further development work. Using a new form of particle swarm optimization that incorporates a strong element of stochastic search, Breeding PSO, it is shown it is possible to use multilayer nets to predict resistance functions for Tri-SWACH hullforms, including one function, the Residual Resistance Coefficient, which was found intractable with previously explored neural network training methods
Phase stability of stress-sensitive Ag2CO3 silver carbonate at high pressures and temperature
Silver carbonate (Ag2CO3) is a material currently used for artificial carbon storage. In this work, we report synchrotron X-ray powder diffraction (XRD) experiments under high pressure and high temperature in combination with density-functional theory (DFT) calculations on silver carbonate up to 13.3 GPa. Two pressure-induced phase transitions were observed at room temperature: at 2.9 GPa to a high-pressure (HP1) phase and at 10.5 GPa to a second high-pressure phase (HP2). The facts that a) the HP2 phase can be indexed with the initial P21/m structure, b) our DFT calculations predict the initial structure is stable in the entire pressure range, and c) the HP2 phase is stable under decompression suggest that the intermediate HP1 phase is a product of the appearance of non-hydrostatic stresses in the sample. The observed structural transformations are associated to a high sensitivity of this compound to non-hydrostatic conditions. The compressibility of Ag2CO3 has also been determined, showing the c axis is the most compressible and that the bulk modulus increases quickly with applied pressure. We attribute both observations to the weak nature of the closed-shell AgâAg interactions in this material. The behavior of Ag2CO3 under heating at approximately 3 GPa was also studied. No temperature-induced phase transitions were found at this pressure, and the thermal expansion was determined to be relatively high for a carbonate.Authors thank the financial support from the Spanish Ministerio de Ciencia e InnovaciĂłn (MICINN) and the Agencia Estatal de InvestigaciĂłn under projects MALTA Consolider Ingenio 2010 network (RED2018-102612-T) and PGC2021-125518NB-I00 (cofinanced by EU FEDER funds), and from the Generalitat Valenciana under projects CIAICO/2021/241 and MFA/2022/007. A.O.R. acknowledges the financial support of the Spanish MINECO RyC-2016-20301 RamĂłn y Cajal Grant and the project AYUD/2021/51036 of the Principality of Asturias (cofinanced by EU FEDER funds). Authors also thank the MALTA Consolider supercomputing centre and Compute Canada for computational resources and ALBA-CELLS synchrotron for providing beamtime under experiments 2020084419 and 2021024988. These experiments were performed at the MSPD beamline with the collaboration of ALBA staff
Emergence of fluoroquinolone-resistant Campylobacter jejuni and Campylobacter coli among Australian chickens in the absence of fluoroquinolone use
In a structured survey of all major chicken-meat producers in Australia, we investigated the antimicrobial resistance (AMR) and genomic characteristics of Campylobacter jejuni (nâ=â108) and C. coli (nâ=â96) from cecal samples of chickens at slaughter (nâ=â200). The majority of the C. jejuni (63%) and C. coli (86.5%) samples were susceptible to all antimicrobials. Fluoroquinolone resistance was detected among both C. jejuni (14.8%) and C. coli (5.2%), although this only included three sequence types (STs) and one ST, respectively. Multidrug resistance among strains of C. jejuni (0.9%) and C. coli (4.1%) was rare, and fluoroquinolone resistance, when present, was never accompanied by resistance to any other agent. Comparative genome analysis demonstrated that Australian isolates were found dispersed on different branches/clusters within the international collection. The major fluoroquinolone-resistant STs of C. jejuni (ST7323, ST2083, and ST2343) and C. coli (ST860) present in Australian chickens were similar to those of international isolates and have been reported previously in humans and animals overseas. The detection of a subpopulation of Campylobacter isolates exclusively resistant to fluoroquinolone was unexpected since most critically important antimicrobials such as fluoroquinolones are excluded from use in Australian livestock. A number of factors, including the low level of resistance to other antimicrobials, the absence of fluoroquinolone use, the adoption of measures for preventing spread of contagion between flocks, and particularly the genomic identities of isolates, all point to humans, pest species, or wild birds as being the most plausible source of organisms. This study also demonstrates the need for vigilance in the form of surveillance for AMR based on robust sampling to manage AMR risks in the food chain
Utility of biotechnology based decision making tools in postharvest grain pest management: an Australian case study
A major concern for the Australian grain industry in recent years is the constant threat of resistance to the key disinfectant phosphine in a range of stored grain pests. The need to maintain the usefulness of phosphine and to contain the development of resistance are critical to international market access for Australian grain. Strong levels of resistance have already been established in major pests including the lesser grain borer, Rhyzopertha dominica (F.), the red flour beetle, Tribolium castaneum (Herbst), and most recently in the rusty grain beetle Cryptolestes ferrugineus (Stephens). As a proactive integrated resistance management strategy, new fumigation protocols are being developed in the laboratory and verified in large-scale field trials in collaboration with industry partners. To aid this development, we have deployed advanced molecular diagnostic tools to accurately determine the strength and frequency of key phosphine resistant insect pests and their movement within a typical Australian grain value chain. For example, two major bulk storage facilities based at Brookstead and Millmerran in southeast Queensland, Australia, were selected as main nodes and several farms and feed mills located in and around these two sites at a scale of 25 to 100 km radius were selected and surveyed. We determined the type, pattern, frequency as well as the distribution of resistance alleles accurately for two major pests, R. dominica and T. castaneum. Overall, this information along with the phenotypic data, provide a basis for designing key intervention strategies in managing resistance problems in the study area
Asteroids' physical models from combined dense and sparse photometry and scaling of the YORP effect by the observed obliquity distribution
The larger number of models of asteroid shapes and their rotational states
derived by the lightcurve inversion give us better insight into both the nature
of individual objects and the whole asteroid population. With a larger
statistical sample we can study the physical properties of asteroid
populations, such as main-belt asteroids or individual asteroid families, in
more detail. Shape models can also be used in combination with other types of
observational data (IR, adaptive optics images, stellar occultations), e.g., to
determine sizes and thermal properties. We use all available photometric data
of asteroids to derive their physical models by the lightcurve inversion method
and compare the observed pole latitude distributions of all asteroids with
known convex shape models with the simulated pole latitude distributions. We
used classical dense photometric lightcurves from several sources and
sparse-in-time photometry from the U.S. Naval Observatory in Flagstaff,
Catalina Sky Survey, and La Palma surveys (IAU codes 689, 703, 950) in the
lightcurve inversion method to determine asteroid convex models and their
rotational states. We also extended a simple dynamical model for the spin
evolution of asteroids used in our previous paper. We present 119 new asteroid
models derived from combined dense and sparse-in-time photometry. We discuss
the reliability of asteroid shape models derived only from Catalina Sky Survey
data (IAU code 703) and present 20 such models. By using different values for a
scaling parameter cYORP (corresponds to the magnitude of the YORP momentum) in
the dynamical model for the spin evolution and by comparing synthetics and
observed pole-latitude distributions, we were able to constrain the typical
values of the cYORP parameter as between 0.05 and 0.6.Comment: Accepted for publication in A&A, January 15, 201
Interobserver agreement on definition of the target volume in stereotactic radiotherapy for pancreatic adenocarcinoma using different imaging modalities
PURPOSE
The aim of this study was to evaluate interobserver agreement (IOA) on target volume definition for pancreatic cancer (PACA) within the Radiosurgery and Stereotactic Radiotherapy Working Group of the German Society of Radiation Oncology (DEGRO) and to identify the influence of imaging modalities on the definition of the target volumes.
METHODS
Two cases of locally advanced PACA and one local recurrence were selected from a large SBRT database. Delineation was based on either a planning 4D CT with or without (w/wo) IV contrast, w/wo PET/CT, and w/wo diagnostic MRI. Novel compared to other studies, a combination of four metrics was used to integrate several aspects of target volume segmentation: the Dice coefficient (DSC), the Hausdorff distance (HD), the probabilistic distance (PBD), and the volumetric similarity (VS).
RESULTS
For all three GTVs, the median DSC was 0.75 (range 0.17-0.95), the median HD 15Â (range 3.22-67.11)Â mm, the median PBD 0.33 (range 0.06-4.86), and the median VS was 0.88 (range 0.31-1). For ITVs and PTVs the results were similar. When comparing the imaging modalities for delineation, the best agreement for the GTV was achieved using PET/CT, and for the ITV and PTV using 4DÂ PET/CT, in treatment position with abdominal compression.
CONCLUSION
Overall, there was good GTV agreement (DSC). Combined metrics appeared to allow a more valid detection of interobserver variation. For SBRT, either 4D PET/CT or 3D PET/CT in treatment position with abdominal compression leads to better agreement and should be considered as a very useful imaging modality for the definition of treatment volumes in pancreatic SBRT. Contouring does not appear to be the weakest link in the treatment planning chain of SBRT for PACA
Utility of biotechnology based decision making tools in postharvest grain pest management: an Australian case study
A major concern for the Australian grain industry in recent years is the constant threat of resistance to the key disinfectant phosphine in a range of stored grain pests. The need to maintain the usefulness of phosphine and to contain the development of resistance are critical to international market access for Australian grain. Strong levels of resistance have already been established in major pests including the lesser grain borer, Rhyzopertha dominica (F.), the red flour beetle, Tribolium castaneum (Herbst), and most recently in the rusty grain beetle Cryptolestes ferrugineus (Stephens). As a proactive integrated resistance management strategy, new fumigation protocols are being developed in the laboratory and verified in large-scale field trials in collaboration with industry partners. To aid this development, we have deployed advanced molecular diagnostic tools to accurately determine the strength and frequency of key phosphine resistant insect pests and their movement within a typical Australian grain value chain. For example, two major bulk storage facilities based at Brookstead and Millmerran in southeast Queensland, Australia, were selected as main nodes and several farms and feed mills located in and around these two sites at a scale of 25 to 100 km radius were selected and surveyed. We determined the type, pattern, frequency as well as the distribution of resistance alleles accurately for two major pests, R. dominica and T. castaneum. Overall, this information along with the phenotypic data, provide a basis for designing key intervention strategies in managing resistance problems in the study area
Dynamic and Assembly Characteristics of Deep-Cavity Basket Acting as a Host for Inclusion Complexation of Mitoxantrone in Biotic and Abiotic Systems
We describe the preparation, dynamic, assembly characteristics of vase-shaped basket 13â along with its ability to form an inclusion complex with anticancer drug mitoxantrone in abiotic and biotic systems. This novel cavitand has a deep nonpolar pocket consisting of three naphthalimide sides fused to a bicyclic platform at the bottom while carrying polar glycines at the top. The results of 1H Nuclear Magnetic Resonance (NMR), 1Hâ
NMR Chemical Exchange Saturation Transfer (CEST), Calorimetry, Hybrid Replica Exchange Molecular Dynamics (REMD), and Microcrystal Electron Diffraction (MicroED) measurements are in line with 1 forming dimer [12]6â, to be in equilibrium with monomers 1(R)3â (relaxed) and 1(S)3â (squeezed). Through simultaneous line-shape analysis of 1Hâ
NMR data, kinetic and thermodynamic parameters characterizing these equilibria were quantified. Basket 1(R)3â includes anticancer drug mitoxantrone (MTO2+) in its pocket to give stable binary complex [MTOâ1]â (Kd=2.1â
ÎŒM) that can be precipitated inâ
vitro with UV light or pH as stimuli. Both inâ
vitro and inâ
vivo studies showed that the basket is nontoxic, while at a higher proportion with respect to MTO it reduced its cytotoxicity inâ
vitro. With well-characterized internal dynamics and dimerization, the ability to include mitoxantrone, and biocompatibility, the stage is set to develop sequestering agents from deep-cavity baskets
Silver(I) complexes with 4,7-phenanthroline efficient in rescuing the zebrafish embryos of lethal Candida albicans infection
Five novel silver(I) complexes with 4,7-phenanthroline (4,7-phen), [Ag(NO3-O)(4,7- phen-ÎŒ-N4,N7)]n (1), [Ag(ClO4-Đ)(4,7-phen-ÎŒ-N4,N7)]n (2), [Ag(CF3COO-O)(4,7- phen-ÎŒ-N4,N7)]n (3), [Ag2(H2O)0.58(4,7-phen)3](SbF6)2 (4) and {[Ag2(H2O)(4,7- phen-ÎŒ-N4,N7)2](BF4)2}n (5) were synthesized, structurally elucidated and biologically evaluated. These complexes showed selectivity towards Candida spp. in comparison to the tested bacteria and effectively inhibited the growth of four different Candida species, particularly of C. albicans strains, with minimal inhibitory concentrations (MICs) in the range of 2.0â10.0âŻÎŒM. In order to evaluate the therapeutic potential of 1â5, in vivo toxicity studies were conducted in the zebrafish model. Based on the favorable therapeutic profiles, complexes 1, 3 and 5 were selected for the evaluation of their antifungal efficacy in vivo using the zebrafish model of lethal disseminated candidiasis. Complexes 1 and 3 efficiently controlled and prevented fungal filamentation even at sub-MIC doses, while drastically increased the survival of the infected embryos. Moreover, at the MIC doses, both complexes totally prevented C. albicans filamentation and rescued almost all infected fish of the fatal infection outcome. On the other side, complex 5, which demonstrated the highest antifungal activity in vitro, affected the neutrophils occurrence of the infected host, failed to inhibit the C. albicans cells filamentation and showed a poor potential to cure candidal infection, highlighting the importance of the in vivo activity evaluation early in the therapeutic design and development process. The mechanism of action of the investigated silver(I) complexes was related to the induction of reactive oxygen species (ROS) response in C. albicans, with DNA being one of the possible target biomolecules
- âŠ