Report of IAU Commission 30 on Radial Velocities (2006-2009)

Brief summaries are given on the following subjects: Radial velocities and exoplanets (Toward Earth-mass planets; Retired A stars and their planets; Current status and prospects); Toward higher radial velocity precision; Radial velocities and asteroseismology; Radial velocities in Galactic and extragalactic clusters; Radial velocities for field giants; Galactic structure -- Large surveys (The Geneva-Copenhagen Survey; Sloan Digital Sky Survey; RAVE); Working groups (WG on radial velocity standards; WG on stellar radial velocity bibliography; WG on the catalogue of orbital elements of spectroscopic binaries [SB9]).Comment: 11 pages, to appear in the IAU Transactions Vol. XXVIIA, Reports on Astronomy 2006-2009, ed. Karel van der Hucht. Editor: G. Torre

Acceleration with Self-Injection for an All-Optical Radiation Source at LNF

We discuss a new compact gamma-ray source aiming at high spectral density, up to two orders of magnitude higher than currently available bremsstrahlung sources, and conceptually similar to Compton Sources based on conventional linear accelerators. This new source exploits electron bunches from laser-driven electron acceleration in the so-called self-injection scheme and uses a counter-propagating laser pulse to obtain X and gamma-ray emission via Thomson/Compton scattering. The proposed experimental configuration inherently provides a unique test-bed for studies of fundamental open issues of electrodynamics. In view of this, a preliminary discussion of recent results on self-injection with the FLAME laser is also given.Comment: 8 pages, 10 figures, 44 references - Channeling 2012 conferenc

Bioink properties before, during and after 3D bioprinting

Bioprinting is a process based on additive manufacturing from materials containing living cells. These materials, often referred to as bioink, are based on cytocompatible hydrogel precursor formulations, which gel in a manner compatible with different bioprinting approaches. The bioink properties before, during and after gelation are essential for its printability, comprising such features as achievable structural resolution, shape fidelity and cell survival. However, it is the final properties of the matured bioprinted tissue construct that are crucial for the end application. During tissue formation these properties are influenced by the amount of cells present in the construct, their proliferation, migration and interaction with the material. A calibrated computational framework is able to predict the tissue development and maturation and to optimize the bioprinting input parameters such as the starting material, the initial cell loading and the construct geometry. In this contribution relevant bioink properties are reviewed and discussed on the example of most popular bioprinting approaches. The effect of cells on hydrogel processing and vice versa is highlighted. Furthermore, numerical approaches were reviewed and implemented for depicting the cellular mechanics within the hydrogel as well as for prediction of mechanical properties to achieve the desired hydrogel construct considering cell density, distribution and material-cell interaction

Sites for Gamma-ray Astronomy in Argentina

We have searched for possible sites in Argentina for the installation of large air Cherenkov telescope arrays and water Cherenkov systems. At present seven candidates are identified at altitudes from 2500 to 4500 m. The highest sites are located at the Northwest of the country, in La Puna. Sites at 2500 and 3100 m are located in the West at El Leoncito Observatory, with excellent infrastructure. A description of these candidate sites is presented with emphasis on infrastructure and climatology.Comment: Submitted to Proceedings of "4th Heidelberg International Symposium on High Energy Gamma-Ray Astronomy 2008

SB9: The Ninth Catalogue of Spectroscopic Binary Orbits

The Ninth Catalogue of Spectroscopic Binary Orbits (http://sb9.astro.ulb.ac.be) continues the series of compilations of spectroscopic orbits carried out over the past 35 years by Batten and collaborators. As of 2004 May 1st, the new Catalogue holds orbits for 2,386 systems. Some essential differences between this catalogue and its predecessors are outlined and three straightforward applications are presented: (1) Completeness assessment: period distribution of SB1s and SB2s; (2) Shortest periods across the H-R diagram; (3) Period-eccentricity relation.Comment: Accepte for publication in A&A, 6 pages, 6 figure

Nerve injury induces robust allodynia and ectopic discharges in Na(v)1.3 null mutant mice

Changes in sodium channel activity and neuronal hyperexcitability contribute to neuropathic pain, a major clinical problem. There is strong evidence that the re-expression of the embryonic voltage-gated sodium channel subunit Na(v)1.3 underlies neuronal hyperexcitability and neuropathic pain. Here we show that acute and inflammatory pain behaviour is unchanged in global Na(v)1.3 mutant mice. Surprisingly, neuropathic pain also developed normally in the Na(v)1.3 mutant mouse. To rule out any genetic compensation mechanisms that may have masked the phenotype, we investigated neuropathic pain in two conditional Na(v)1.3 mutant mouse lines. We used Na(v)1.8-Cre mice to delete Nav1.3 in nociceptors at E14 and NFH-Cre mice to delete Na(v)1.3 throughout the nervous system postnatally. Again normal levels of neuropathic pain developed after nerve injury in both lines. Furthermore, ectopic discharges from damaged nerves were unaffected by the absence of Na(v)1.3 in global knock-out mice. Our data demonstrate that Na(v)1.3 is neither necessary nor sufficient for the development of nerve-injury related pain

Chondrogenic potential of chondrocytes in hyaluronic acid/PEG-based hydrogels is dependent on the hyaluronic acid concentration

Purpose: Hydrogels based on PEG and methacrylated poly(N-(2-hydroxypropyl) methacrylamide-mono/dilactate) (M10P10) are promising biomaterials for Biofabrication of cartilage constructs. Addition of hyaluronic acid (HA) to a hydrogel improves printability by increasing the viscosity. Methacrylating HA (HAMA) can ensure covalent binding in M10P10 hydrogels after UV-cross-linking. Chondrocytes can interact with HAMA via their CD44 receptor, however, the influence of HAMA on chondrogenic potential is unclear. This study aimed to evaluate the influence of different HAMA concentrations on chondrogenesis of chondrocytes in M10P10/HAMA hydrogels. Materials & Methods: Equine chondrocytes were encapsulated in M10P10 hydrogels containing different HAMA concentrations. Cylindrical constructs were cast, UV-cross-linked, and cultured in TGF-β-containing medium. Constructs were analyzed for evidence of cartilage formation. Results: Preliminary data showed an increase in glycosaminoglycan (GAG)/DNA for constructs with low HAMA concentrations (0.1-0.25%) while no differences were found for higher HAMA concentrations, compared to hydrogels without HAMA (Figure 1a). Further, constructs without or with low HAMA concentrations (0.1-0.5%) demonstrated collagen type II positive areas, while this was less pronounced in constructs with 0.5-1% HAMA (n=3, Figure 1b). Conclusion: Preliminary results indicate a dose-dependent effect of HAMA on chondrogenesis of chondrocytes: low concentrations (0.1-0.25%) increase GAG production while higher concentrations (0.5-1%) have no effect on GAG production and reduce collagen type II synthesis. Ongoing evaluations will reveal the extent of chondrogenesis and its association with HAMA concentrations in M10P10/HAMA, and the mechanism responsible for the dose-dependent effect. This study will impact the use of HAMA as viscosity enhancer to improve the printability of hydrogel

A Stimuli-Responsive Nanocomposite for 3D Anisotropic Cell-Guidance and Magnetic Soft Robotics

Stimuli-responsive materials have the potential to enable the generation of new bioinspired devices with unique physicochemical properties and cell-instructive ability. Enhancing biocompatibility while simplifying the production methodologies, as well as enabling the creation of complex constructs, i.e., via 3D (bio)printing technologies, remains key challenge in the field. Here, a novel method is presented to biofabricate cellularized anisotropic hybrid hydrogel through a mild and biocompatible process driven by multiple external stimuli: magnetic field, temperature, and light. A low-intensity magnetic field is used to align mosaic iron oxide nanoparticles (IOPs) into filaments with tunable size within a gelatin methacryloyl matrix. Cells seeded on top or embedded within the hydrogel align to the same axes of the IOPs filaments. Furthermore, in 3D, C2C12 skeletal myoblasts differentiate toward myotubes even in the absence of differentiation media. 3D printing of the nanocomposite hydrogel is achieved and creation of complex heterogeneous structures that respond to magnetic field is demonstrated. By combining the advanced, stimuli-responsive hydrogel with the architectural control provided by bioprinting technologies, 3D constructs can also be created that, although inspired by nature, express functionalities beyond those of native tissue, which have important application in soft robotics, bioactuators, and bionic devices

Equipotential Surfaces and Lagrangian points in Non-synchronous, Eccentric Binary and Planetary Systems

We investigate the existence and properties of equipotential surfaces and Lagrangian points in non-synchronous, eccentric binary star and planetary systems under the assumption of quasi-static equilibrium. We adopt a binary potential that accounts for non-synchronous rotation and eccentric orbits, and calculate the positions of the Lagrangian points as functions of the mass ratio, the degree of asynchronism, the orbital eccentricity, and the position of the stars or planets in their relative orbit. We find that the geometry of the equipotential surfaces may facilitate non-conservative mass transfer in non-synchronous, eccentric binary star and planetary systems, especially if the component stars or planets are rotating super-synchronously at the periastron of their relative orbit. We also calculate the volume-equivalent radius of the Roche lobe as a function of the four parameters mentioned above. Contrary to common practice, we find that replacing the radius of a circular orbit in the fitting formula of Eggleton (1983) with the instantaneous distance between the components of eccentric binary or planetary systems does not always lead to a good approximation to the volume-equivalent radius of the Roche-lobe. We therefore provide generalized analytic fitting formulae for the volume-equivalent Roche lobe radius appropriate for non-synchronous, eccentric binary star and planetary systems. These formulae are accurate to better than 1% throughout the relevant 2-dimensional parameter space that covers a dynamic range of 16 and 6 orders of magnitude in the two dimensions.Comment: 12 pages, 10 figures, 2 Tables, Accepted by the Astrophysical Journa