Thermodynamics of nano-spheres encapsulated in virus capsids

We investigate the thermodynamics of complexation of functionalized charged nano-spheres with viral proteins. The physics of this problem is governed by electrostatic interaction between the proteins and the nano-sphere cores (screened by salt ions), but also by configurational degrees of freedom of the charged protein N-tails. We approach the problem by constructing an appropriate complexation free energy functional. On the basis of both numerical and analytical studies of this functional we construct the phase diagram for the assembly which contains the information on the assembled structures that appear in the thermodynamical equilibrium, depending on the size and surface charge density of the nano-sphere cores. We show that both the nano-sphere core charge as well as its radius determine the size of the capsid that forms around the core.Comment: Submitte

Thermochromic display materials for use under wide variations in ambient illumination levels Final report

Inorganic thermochromatic material synthesis and utilization in display device

Density waves theory of the capsid structure of small icosahedral viruses

We apply Landau theory of crystallization to explain and to classify the capsid structures of small viruses with spherical topology and icosahedral symmetry. We develop an explicit method which predicts the positions of centers of mass for the proteins constituting viral capsid shell. Corresponding density distribution function which generates the positions has universal form without any fitting parameter. The theory describes in a uniform way both the structures satisfying the well-known Caspar and Klug geometrical model for capsid construction and those violating it. The quasiequivalence of protein environments in viral capsid and peculiarities of the assembly thermodynamics are also discussed.Comment: 8 pages, 3 figur

Notorious places: image, reputation, stigma: the role of newspapers in area reputations for social housing estates

This paper reviews work in several disciplines to distinguish between image, reputation and stigma. It also shows that there has been little research on the process by which area reputations are established and sustained through transmission processes. This paper reports on research into the portrayal of two social housing estates in the printed media over an extended period of time (14 years). It was found that negative and mixed coverage of the estates dominated, with the amount of positive coverage being very small. By examining the way in which dominant themes were used by newspapers in respect of each estate, questions are raised about the mode of operation of the press and the communities' collective right to challenge this. By identifying the way regeneration stories are covered and the nature of the content of positive stories, lessons are drawn for programmes of area transformation. The need for social regeneration activities is identified as an important ingredient for changing deprived-area reputations

Kinetics of viral self-assembly: the role of ss RNA antenna

A big class of viruses self-assemble from a large number of identical capsid proteins with long flexible N-terminal tails and ss RNA. We study the role of the strong Coulomb interaction of positive N-terminal tails with ss RNA in the kinetics of the in vitro virus self-assembly. Capsid proteins stick to unassembled chain of ss RNA (which we call "antenna") and slide on it towards the assembly site. We show that at excess of capsid proteins such one-dimensional diffusion accelerates self-assembly more than ten times. On the other hand at excess of ss RNA, antenna slows self-assembly down. Several experiments are proposed to verify the role of ss RNA antenna.Comment: 4 pages, 3 figures, several experiments are proposed, a new idea of experiment is adde

A Study of Lyman-Alpha Quasar Absorbers in the Nearby Universe

Spectroscopy of ten quasars obtained with the Goddard High Resolution Spectrograph (GHRS) of the Hubble Space Telescope (HST) is presented. A clustering analysis reveals an excess of nearest neighbor line pairs on velocity scales of 250-750 km/s at a 95-98% confidence level. The hypothesis that the absorbers are randomly distributed in velocity space can be ruled out at the 99.8% confidence level. No two-point correlation power is detected (xi < 1 with 95% confidence). Lyman-alpha absorbers have correlation amplitudes on scales of 250-500 km/s at least 4-5 times smaller than the correlation amplitude of bright galaxies. A detailed comparison between absorbers in nearby galaxies is carried out on a limited subset of 11 Lyman- alpha absorbers where the galaxy sample in a large contiguous volume is complete to M_B = -16. Absorbers lie preferentially in regions of intermediate galaxy density but it is often not possible to uniquely assign a galaxy counterpart to an absorber. This sample provides no explicit support for the hypothesis that absorbers are preferentially associated with the halos of luminous galaxies. We have made a preliminary comparison of the absorption line properties and environments with the results of hydrodynamic simulations. The results suggest that the Lyman-alpha absorbers represent diffuse or shocked gas in the IGM that traces the cosmic web of large scale structure. (abridged)Comment: 36 pages of text, 15 figures, 4 tables, 36 file

Polysaccharide utilization loci and nutritional specialization in a dominant group of butyrate-producing human colonic Firmicutes

Acknowledgements The Rowett Institute of Nutrition and Health (University of Aberdeen) receives financial support from the Scottish Government Rural and Environmental Sciences and Analytical Services (RESAS). POS is a PhD student supported by the Scottish Government (RESAS) and the Science Foundation Ireland, through a centre award to the APC Microbiome Institute, Cork, Ireland. Data Summary The high-quality draft genomes generated in this work were deposited at the European Nucleotide Archive under the following accession numbers: 1. Eubacterium rectale T1-815; CVRQ01000001–CVRQ0100 0090: http://www.ebi.ac.uk/ena/data/view/PRJEB9320 2. Roseburia faecis M72/1; CVRR01000001–CVRR010001 01: http://www.ebi.ac.uk/ena/data/view/PRJEB9321 3. Roseburia inulinivorans L1-83; CVRS01000001–CVRS0 100 0151: http://www.ebi.ac.uk/ena/data/view/PRJEB9322Peer reviewedPublisher PD