Carbon assimilation strategies in ultrabasic groundwater: clues from the integrated study of a serpentinization-influenced aquifer

© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Seyler, L. M., Brazelton, W. J., McLean, C., Putman, L. I., Hyer, A., Kubo, M. D. Y., Hoehler, T., Cardace, D., & Schrenk, M. O. . Carbon assimilation strategies in ultrabasic groundwater: clues from the integrated study of a serpentinization-influenced aquifer. mSystems, 5(2), (2020): e00607-00619, doi: 10.1128/mSystems.00607-19.Serpentinization is a low-temperature metamorphic process by which ultramafic rock chemically reacts with water. Such reactions provide energy and materials that may be harnessed by chemosynthetic microbial communities at hydrothermal springs and in the subsurface. However, the biogeochemistry mediated by microbial populations that inhabit these environments is understudied and complicated by overlapping biotic and abiotic processes. We applied metagenomics, metatranscriptomics, and untargeted metabolomics techniques to environmental samples taken from the Coast Range Ophiolite Microbial Observatory (CROMO), a subsurface observatory consisting of 12 wells drilled into the ultramafic and serpentinite mélange of the Coast Range Ophiolite in California. Using a combination of DNA and RNA sequence data and mass spectrometry data, we found evidence for several carbon fixation and assimilation strategies, including the Calvin-Benson-Bassham cycle, the reverse tricarboxylic acid cycle, the reductive acetyl coenzyme A (acetyl-CoA) pathway, and methylotrophy, in the microbial communities inhabiting the serpentinite-hosted aquifer. Our data also suggest that the microbial inhabitants of CROMO use products of the serpentinization process, including methane and formate, as carbon sources in a hyperalkaline environment where dissolved inorganic carbon is unavailable.We thank McLaughlin Reserve, in particular Paul Aigner and Cathy Koehler, for hosting sampling at CROMO and providing access to the wells, A. Daniel Jones and Anthony Schilmiller for their advice regarding metabolite extraction and mass spectrometry, Elizabeth Kujawinski for her guidance in metabolomics data analysis and interpretation, and Julia McGonigle, Christopher Thornton, and Katrina Twing for assistance with metagenomic and computational analyses

Pion parameters in nuclear medium from chiral perturbation theory and virial expansion

We consider two methods to find the effective parameters of the pion traversing a nuclear medium. One is the first order chiral perturbation theoretic evaluation of the pion pole contribution to the two-point function of the axial-vector current. The other is the exact, first order virial expansion of the pion self-energy. We find that, although the results of chiral perturbation theory are not valid at normal nuclear density, those from the virial expansion may be reliable at such density. The latter predicts both the mass-shift and the in-medium decay width of the pion to be small, of about a few MeV.Comment: 9 Pages RevTex, 3 eps figure

Neutron charge form factor at large q2q^2

The neutron charge form factor $G_{En}(q)$ is determined from an analysis of the deuteron quadrupole form factor $F_{C2}$ data. Recent calculations, based on a variety of different model interactions and currents, indicate that the contributions associated with the uncertain two-body operators of shorter range are relatively small for $F_{C2}$, even at large momentum transfer $q$. Hence, $G_{En}(q)$ can be extracted from $F_{C2}$ at large $q^2$ without undue systematic uncertainties from theory.Comment: 8 pages, 3 figure

Covariant and Heavy Quark Symmetric Quark Models

There exist relativistic quark models (potential or MIT-bag) which satisfy the heavy quark symmetry (HQS) relations among meson decay constants and form factors. Covariant construction of the momentum eigenstates, developed here, can correct for spurious center-of-mass motion contributions.Proton form factor and M1 transitions in quarkonia are calculated. Explicit expression for the Isgur-Wise function is found and model determined deviations from HQS are studied. All results depend on the model parameters only. No additional ad hoc assumptions are needed.Comment: 34 pages (2 figures not included but avaliable upon request), LATEX, (to be published in Phys.Rev.D

A model for two-proton emission induced by electron scattering

A model to study two-proton emission processes induced by electron scattering is developed. The process is induced by one-body electromagnetic operators acting together with short-range correlations, and by two-body $\Delta$ currents. The model includes all the diagrams containing a single correlation function. A test of the sensitivity of the model to the various theoretical inputs is done. An investigation of the relevance of the $\Delta$ currents is done by changing the final state angular momentum, excitation energy and momentum transfer. The sensitivity of the cross section to the details of the correlation function is studied by using realistic and schematic correlations. Results for $^{12}$C, $^{16}$O and $^{40}$Ca nuclei are presented.Comment: 30 pages, 18 figures, 3 table

Superscaling of Inclusive Electron Scattering from Nuclei

We investigate the degree to which the concept of superscaling, initially developed within the framework of the relativistic Fermi gas model, applies to inclusive electron scattering from nuclei. We find that data obtained from the low energy loss side of the quasielastic peak exhibit the superscaling property, i.e., the scaling functions f(\psi') are not only independent of momentum transfer (the usual type of scaling: scaling of the first kind), but coincide for A \geq 4 when plotted versus a dimensionless scaling variable \psi' (scaling of the second kind). We use this behavior to study as yet poorly understood properties of the inclusive response at large electron energy loss.Comment: 33 pages, 12 color EPS figures, LaTeX2e using BoxedEPSF macros; email to [email protected]

The Decuplet Revisited in χ\chiPT

The paper deals with two issues. First, we explore the quantitiative importance of higher multiplets for properties of the $\Delta$ decuplet in chiral perturbation theory. In particular, it is found that the lowest order one--loop contributions from the Roper octet to the decuplet masses and magnetic moments are substantial. The relevance of these results to the chiral expansion in general is discussed. The exact values of the magnetic moments depend upon delicate cancellations involving ill--determined coupling constants. Second, we present new relations between the magnetic moments of the $\Delta$ decuplet that are independent of all couplings. They are exact at the order of the chiral expansion used in this paper.Comment: 7 pages of double column revtex, no figure

Controls on methane concentration and stable isotope (δ2H-CH4 and δ13C-CH4) distributions in the water columns of the Black Sea and Cariaco Basin

Methane (CH4) concentration and stable isotope (δ2H-CH4 and δ13C-CH4) depth distributions show large differences in the water columns of the Earth's largest CH4-containing anoxic basins, the Black Sea and Cariaco Basin. In the deep basins, the between-basin stable isotope differences are large, 83‰ for δ2H-CH4 and 9‰ for δ13C-CH4, and the distributions are mirror images of one another. The major sink in both basins, anaerobic oxidation of CH4, results in such extensive isotope fractionation that little direct information can be obtained regarding sources. Recent measurements of natural 14C-CH4 show that the CH4 geochemistry in both basins is dominated (∼64 to 98%) by inputs of fossil (radiocarbon-free) CH4 from seafloor seeps. We derive open-system kinetic isotope effect equations and use a one-dimensional (vertical) stable isotope box model that, along with isotope budgets developed using radiocarbon, permits a quantitative treatment of the stable isotope differences. We show that two main factors control the CH4 concentration and stable isotope differences: (1) the depth distributions of the input of CH4 from seafloor seeps and (2) anaerobic oxidation of CH4 under open-system steady state conditions in the Black Sea and open-system non-steady-state conditions in the Cariaco Basin

Infinite Nuclear Matter on the Light Front: Nucleon-Nucleon Correlations

A relativistic light front formulation of nuclear dynamics is developed and applied to treating infinite nuclear matter in a method which includes the correlations of pairs of nucleons: this is light front Brueckner theory. We start with a hadronic meson-baryon Lagrangian that is consistent with chiral symmetry. This is used to obtain a light front version of a one-boson-exchange nucleon-nucleon potential (OBEP). The accuracy of our description of the nucleon-nucleon (NN) data is good, and similar to that of other relativistic OBEP models. We derive, within the light front formalism, the Hartree-Fock and Brueckner Hartree-Fock equations. Applying our light front OBEP, the nuclear matter saturation properties are reasonably well reproduced. We obtain a value of the compressibility, 180 MeV, that is smaller than that of alternative relativistic approaches to nuclear matter in which the compressibility usually comes out too large. Because the derivation starts from a meson-baryon Lagrangian, we are able to show that replacing the meson degrees of freedom by a NN interaction is a consistent approximation, and the formalism allows one to calculate corrections to this approximation in a well-organized manner. The simplicity of the vacuum in our light front approach is an important feature in allowing the derivations to proceed. The mesonic Fock space components of the nuclear wave function are obtained also, and aspects of the meson and nucleon plus-momentum distribution functions are computed. We find that there are about 0.05 excess pions per nucleon.Comment: 39 pages, RevTex, two figure