Therapy for Irregular Menstruation with Acupunture and Herbal Pegagan (Centella Asiatica (L.))

Background : Menstruation is bleeding periodic scale and cyclical from uterus extrication (deskuamasi) endometrium. Menstruation bleeding is complex interaction result wich involve hormone system and body system, that are hypothalamus, hypofisis, ovarium and uterus. Every woman has different menstruation cycle. The causes of irregular menstruation are genetic and bad habit life. While according to TCM, irregular menstruation is Yue Jing Bu Tiao. Purpose : to knowing the effect of acupuncture therapy and point of Guanyuan (CV 4), Shenshu (BL 23), Sanyinjao (SP 6) and Shenmen (HT 7). Therapy was given 12 times, 3 times every week. Herbal therapy wich given was pegagan (Centella asiatica (L)) with dose 0,3 and consumed everyday after eat. Result : therapy that used acupuncture and herbal in irregular menstruation case showing menstruation cycle change, that was menstruation cycle became shorter from 76 days to 41 days. Conclusion : acupuncture therapy on point of Guanyuan (CV 4), Shenshu (BL 23), Sanyinjao (SP 6), Shenmen (HT 7), and pegagan herbal (Centella asiatica (L)) could shorten menstruation cycle from 76 days to 41 days

DNA damage among wood workers assessed with the comet assay

Exposure to wood dust, a human carcinogen, is common in wood-related industries, and millions of workers are occupationally exposed to wood dust worldwide. The comet assay is a rapid, simple, and sensitive method for determining DNA damage. The objective of this study was to investigate the DNA damage associated with occupational exposure to wood dust using the comet assay (peripheral blood samples) among nonsmoking wood workers (n = 31, furniture and construction workers) and controls (n = 19). DNA damage was greater in the group exposed to composite wood products compared to the group exposed to natural woods and controls (P < 0.001). No difference in DNA damage was observed between workers exposed to natural woods and controls (P = 0.13). Duration of exposure and current dust concentrations had no effect on DNA damage. In future studies, workers' exposures should include cumulative dust concentrations and exposures originating from the binders used in composite wood products

Dental Shape Variation and Phylogenetic Signal in the Rattini Tribe Species of Mainland Southeast Asia

We would like to thank Pierre-Henri Fabre for providing the phylogeny for this study. The collection of specimens used was funded by the French ANR Biodiversity, grant ANR 07 BDIV 012 CERoPath project (www.ceropath.org), and by the French ANR CP&ES, grant ANR 11 CPEL 002 BiodivHealthSEA project (www.biodivhealthsea.org). We also thank Madoudou Garba and Gauthier Dobigny (CBGP-IRD) for providing additional specimens. We greatly thank all local communities and their leaders for permission and invaluable help during field trapping. Special thanks to the CERoPath and BiodivHealthSEA teams and the drivers for their invaluable help during fieldwork. We would also like to thank Maeve McMahon for help with manuscript editing and preparation.Peer reviewedPublisher PD

Pitfalls and improvements in the joint inference of heteroscedasticity and autocorrelation in hydrological model calibration

Residual errors of hydrological models are usually both heteroscedastic and autocorrelated. However, only a few studies have attempted to explicitly include these two statistical properties into the residual error model and jointly infer them with the hydrological model parameters. This technical note shows that applying autoregressive error models to raw heteroscedastic residuals, as done in some recent studies, can lead to unstable error models with poor predictive performance. This instability can be avoided by applying the autoregressive process to standardized residuals. The theoretical analysis is supported by empirical findings in three hydrologically distinct catchments. The case studies also highlight strong interactions between the parameters of autoregressive residual error models and the water balance parameters of the hydrological model. ©2013. American Geophysical Union. All Rights Reserved.Guillaume Evin, Dmitri Kavetski, Mark Thyer, and George Kuczer

Effect of stocker management program on beef cattle skeletal muscle growth characteristics, satellite cell activity, and paracrine signaling impact on preadipocyte differentiation

The objective of this study was to determine the effect of different stocker management programs on skeletal muscle development and growth characteristics, satellite cell (SC) activity in growing-finishing beef cattle as well as the effects of SC-conditioned media on preadipocyte gene expression and differentiation. Fall-weaned Angus steers (n = 76; 258 ± 28 kg) were randomly assigned to 1 of 4 stocker production systems: 1) grazing dormant native range (NR) supplemented with a 40% CP cottonseed meal-based supplement (1.02 kg ∙ steer–1 ∙ d–1) followed by long-season summer grazing (CON, 0.46 kg/d); 2) grazing dormant NR supplemented with a ground corn and soybean meal-based supplement fed at 1% of BW followed by short-season summer grazing (CORN, 0.61 kg/d); 3) grazing winter wheat pasture (WP) at high stocking density (3.21 steers/ha) to achieve a moderate rate of gain (LGWP, 0.83 kg/d); and 4) grazing winter WP at low stocking density (0.99 steers/ha) to achieve a high rate of gain (HGWP, 1.29 kg/d). At the end of the stocker (intermediate harvest, IH) and finishing (final harvest, FH) phases, 4 steers / treatment were harvested and longissimus muscles (LM) sampled for cryohistological immunofluorescence analysis and SC culture assays. At IH, WP steers had greater LM fiber cross-sectional area than NR steers; however, at FH, the opposite was observed (p \u3c 0.0001). At IH, CORN steers had the lowest Myf-5+:Pax7+ SC density (p = 0.020), while LGWP steers had the most Pax7+ SC (p = 0.043). At FH, CON steers had the highest LM capillary density (p = 0.003) and their cultured SC differentiated more readily than all other treatments (p = 0.017). At FH, Pax7 mRNA was more abundant in 14 d-old SC cultures from HGWP cattle (p = 0.03). Preadipocytes exposed to culture media from proliferating SC cultures from WP cattle isolated at FH had more PPARγ (p = 0.037) and less FABP4 (p = 0.030) mRNA expression compared with NR cattle. These data suggest that different stocker management strategies can impact skeletal muscle growth, SC function, and potentially impact marbling development in growing-finishing beef cattle

The Effector Domain of MARCKS Is a Nuclear Localization Signal that Regulates Cellular PIP2 Levels and Nuclear PIP2 Localization

Translocation to the nucleus of diacylglycerol kinase (DGK)– ζ is dependent on a sequence homologous to the effector domain of Myristoylated Alanine Rich C-Kinase Substrate (MARCKS). These data would suggest that MARCKS could also localize to the nucleus. A single report demonstrated immunofluorescence staining of MARCKS in the nucleus; however, further experimental evidence confirming the specific domain responsible for this localization has not been reported. Here, we report that MARCKS is present in the nucleus in GBM cell lines. We then over-expressed wild-type MARCKS (WT) and MARCKS with the effector domain deleted (ΔED), both tagged with V5-epitope in a GBM cell line with low endogenous MARCKS expression (U87). We found that MARCKS-WT localized to the nucleus, while the MARCKS construct without the effector domain remained in the cytoplasm. We also found that over-expression of MARCKS-WT resulted in a significant increase in total cellular phosphatidyl-inositol (4,5) bisphosphate (PIP2) levels, consistent with prior evidence that MARCKS can regulate PIP2 levels. We also found increased staining for PIP2 in the nucleus with MARCKS-WT over-expression compared to MARCKS ΔED by immunofluorescence. Interestingly, we observed MARCKS and PIP2 co-localization in the nucleus. Lastly, we found changes in gene expression when MARCKS was not present in the nucleus (MARCKS ΔED). These data indicate that the MARCKS effector domain can function as a nuclear localization signal and that this sequence is critical for the ability of MARCKS to regulate PIP2 levels, nuclear localization, and gene expression. These data suggests a novel role for MARCKS in regulating nuclear functions such as gene expression

A doubly stochastic rainfall model with exponentially decaying pulses

We develop a doubly stochastic point process model with exponentially decaying pulses to describe the statistical properties of the rainfall intensity process. Mathematical formulation of the point process model is described along with second-order moment characteristics of the rainfall depth and aggregated processes. The derived second-order properties of the accumulated rainfall at different aggregation levels are used in model assessment. A data analysis using 15 years of sub-hourly rainfall data from England is presented. Models with fixed and variable pulse lifetime are explored. The performance of the model is compared with that of a doubly stochastic rectangular pulse model. The proposed model fits most of the empirical rainfall properties well at sub-hourly, hourly and daily aggregation levels

Evaluation of hydrological models on small mountainous catchments: impact of the meteorological forcings

Hydrological modelling of small mountainous catchments is particularly challenging because of the high spatio-temporal resolution required for the meteorological forcings. In situ measurements of precipitation are typically scarce in these remote areas, particularly at high elevations. Precipitation reanalyses propose different alternative forcings for the simulation of streamflow using hydrological models. In this paper, we evaluate the performances of two hydrological models representing some of the key processes for small mountainous catchments (&lt; 300 km2), using different meteorological products with a fine spatial and temporal resolution. The evaluation is performed on 55 small catchments of the northern French Alps. While the simulated streamflows are adequately reproduced for most of the configurations, these evaluations emphasize the added value of radar measurements, in particular for the reproduction of flood events. However, these better performances are only obtained because the hydrological models correct the underestimations of accumulated amounts (e.g. annual) from the radar data in high-elevation areas.</p