Chloroplast DNA Inheritance in the Orchid Anacamptis palustris Using Single-Seed Polymerase Chain Reaction

The modality of chloroplast inheritance in orchids has been investigated only in a few species due to the difficulties associated with the analysis of large progeny numbers from experimental crosses. To test chloroplast DNA inheritance in the orchid Anacamptis palustris, we took advantage of the presence of a highly variable minisatellite repeat located in the tRNALEU intron in the chloroplast genome. Seed progeny obtained from experimental crosses between parental individuals carrying different chloroplast DNA (cpDNA) minisatellite repeat numbers were analyzed using a single-seed polymerase chain reaction (PCR) protocol. All examined seeds displayed the maternal cpDNA haplotypes, indicating that cpDNA inheritance is strictly maternal in this Mediterranean orchid species. No evidence for paternal leakage was found. This finding concurs with results obtained from PCR amplifications of pollen massulae that exclude the presence of chloroplast DNA in the pollen tetrad

Hong-Ou-Mandel interference between independent III-V on silicon waveguide integrated lasers

The versatility of silicon photonic integrated circuits has led to a widespread usage of this platform for quantum information based applications, including Quantum Key Distribution (QKD). However, the integration of simple high repetition rate photon sources is yet to be achieved. The use of weak-coherent pulses (WCPs) could represent a viable solution. For example, Measurement Device Independent QKD (MDI-QKD) envisions the use of WCPs to distill a secret key immune to detector side channel attacks at large distances. Thus, the integration of III-V lasers on silicon waveguides is an interesting prospect for quantum photonics. Here, we report the experimental observation of Hong-Ou-Mandel interference with 46\pm 2% visibility between WCPs generated by two independent III-V on silicon waveguide integrated lasers. This quantum interference effect is at the heart of many applications, including MDI-QKD. Our work represents a substantial first step towards an implementation of MDI-QKD fully integrated in silicon, and could be beneficial for other applications such as standard QKD and novel quantum communication protocols.Comment: 5 pages, 3 figure

Dissecting the genetic basis for seed coat mucilage heteroxylan biosynthesis in plantago ovata using gamma irradiation and infrared spectroscopy

Seeds from the myxospermous species Plantago ovata release a polysaccharide-rich mucilage upon contact with water. This seed coat derived mucilage is composed predominantly of heteroxylan (HX) and is utilized as a gluten-free dietary fiber supplement to promote human colorectal health. In this study, a gamma-irradiated P. ovata population was generated and screened using histological stains and Fourier Transform Mid Infrared (FTMIR) spectroscopy to identify putative mutants showing defects in seed coat mucilage HX composition and/or structure. FTMIR analysis of dry seed revealed variation in regions of the IR spectra previously linked to xylan structure in Secale cereale (rye). Subsequent absorbance ratio and PCA multivariate analysis identified 22 putative mutant families with differences in the HX IR fingerprint region. Many of these showed distinct changes in the amount and subtle changes in structure of HX after mucilage extrusion, while 20% of the putative HX mutants identified by FTMIR showed no difference in staining patterns of extruded mucilage compared to wild-type. Transcriptional screening analysis of two putative reduced xylan in mucilage (rxm) mutants, rxm1 and rxm3, revealed that changes in HX levels in rxm1 correlate with reduced transcription of known and novel genes associated with xylan synthesis, possibly indicative of specific co-regulatory units within the xylan biosynthetic pathway. These results confirm that FTMIR is a suitable method for identifying putative mutants with altered mucilage HX composition in P. ovata, and therefore forms a resource to identify novel genes involved in xylan biosynthesis.Matthew R. Tucker, Chao Ma, Jana Phan, Kylie Neumann, Neil J. Shirley, Michael G. Hahn, Daniel Cozzolino and Rachel A. Burto

Intermolecular disulfide bond influences unphosphorylated STAT3 dimerization and function

Signal transducer and activator of transcription 3 (STAT3) is a transcription factor activated by the phosphorylation of tyrosine 705 in response to many cytokines and growth factors. Recently, the roles for unphosphorylated STAT3 (U-STAT3) have been described in response to cytokine stimulation, in cancers, and in the maintenance of heterochromatin stability. It has been reported that U-STAT3 dimerizes, shuttles between the cytoplasm and nucleus, and binds to DNA, thereby driving genes transcription. Although many reports describe the active role of U-STAT3 in oncogenesis in addition to phosphorylated STAT3, the U-STAT3 functional pathway remains elusive.In this report, we describe the molecular mechanism of U-STAT3 dimerization, and we identify the presence of two intermolecular disulfide bridges between Cys367 and Cys542 and Cys418 and Cys426, respectively. Recently, we reported that the same cysteines contribute to the redox regulation of STAT3 signaling pathway both in vitro and in vivo The presence of these disulfides is here demonstrated to largely contribute to the structure and the stability of U-STAT3 dimer as the dimeric form rapidly dissociates upon reduction in the S-S bonds. In particular, the Cys367-Cys542 disulfide bridge is shown to be critical for U-STAT3 DNA-binding activity. Mutation of the two Cys residues completely abolishes the DNA-binding capability of U-STAT3. Spectroscopic investigations confirm that the noncovalent interactions are sufficient for proper folding and dimer formation, but that the interchain disulfide bonds are crucial to preserve the functional dimer. Finally, we propose a reaction scheme of U-STAT3 dimerization with a first common step followed by stabilization through the formation of interchain disulfide bond

Finite element analysis of localised rolling to reduce residual stress and distortion

Fusion welding processes cause residual stress due to the uneven heat distribution produced by the moving welding torch. These residual stresses are characterised by a large tensile component in the welding direction. Due to the self-equilibrated nature of the residual stress, compressive ones are present in the far field next to the weld seam, which can cause different kind of distortion such as bending or buckling. Welding residual stress can be responsible of premature failure of the components, such as stress crack corrosion, buckling, and reduction of fatigue life. Localised rolling is a stress engineering technique that can be used to reduce the residual stress and distortion caused by welding. It induces plastic strain in the rolling direction, counteracting the plastic strain produced during welding. In this thesis three techniques were investigated, pre-weld rolling, post-weld rolling, and in situ rolling. These techniques have been seldom studied in the past, particularly pre-weld rolling; consequently the mechanisms are poorly understood. Finite element models allow stress and strain development during both welding and rolling processes to be better understood, providing an improved understanding of the mechanisms involved and aiding process development. A literature survey was done to find the state of the art of the computational welding mechanics simulations, stress management, and the residual stress measurement techniques, as well as the knowledge gaps such as, the thermal losses through the backing-bar in the thermal simulation, the frictional interaction in the rolling process, and the material properties of the steel used in the models. In the literature not many models that investigate the management of welding residual stress were found. After this, the general considerations and assumptions for the welding thermal mechanical models presented in this thesis were discussed. The effect of different backing-bar conditions, as well as different material properties where investigated. Both influenced the residual stress profile to varying degrees. In particular, temperature dependent heat loss to the backing-bar was necessary to capture the improved heat loss near the weld. The distortion predicted by the model was investigated to determine whether it was due to bending or buckling phenomena. Lastly, the temperature distribution and residual stress predictions were validated against thermocouple and neutron diffraction measurements conducted by Coules et al. [1–3]. Pre-weld rolling was the first of the three rolling methods considered, in which rolling is applied to the plates before performing GMA butt-welds. The principle behind this technique consisted in inducing tensile residual stress in the weld region before welding; therefore, it is similar to mechanically tensioning the weld, which can significantly reduce the residual stress and distortion. However, there was no significant change in the tensile residual stresses. On the other hand, it was possible to achieve a small reduction in the distortion, when the plates were rolled on the opposite surface to the weld; rolling in this way induced distortion in the opposite direction to the distortion induced by welding, reducing the magnitude of the latter. These results were compared with experiments conducted by Coules et al. [1,4]. A subsequent investigation combined pre-weld rolling with post-weld heating. With this additional process the residual stress and distortion were significantly reduced, and flatter residual stress profile was achieved. The post-weld rolling and in situ rolling techniques were discussed afterwards. In the post-weld rolling models, rolling was applied after the weldment was cooled to room temperature. In in situ rolling the roller was applied on top of the weld bead at some distance behind the torch, while it was still hot. The principle behind these techniques consisted in applying positive plastic strain to the weld bead region by a roller, counteracting the negative plastic strains produced in the welding process. Two roller profiles were investigated, namely, grooved, and double flat rollers. The post-weld rolling on top of the weld bead models, which used the grooved roller, showed good agreement against experimental results, producing a large reduction of the residual stress and distortion. Some discrepancies were present when the weld toes were rolled with the dual flat roller. The former roller was more efficient for reducing residual stress and distortion. The influence of different friction coefficients (between the roller and weldment, and between the backing-bar and the weldment), were investigated. It showed significant dependency on the residual stress distribution when high rolling loads were used. The frictional interaction constrained the contact area inducing more compressive stress in the core of the weld bead; therefore it produced more tensile residual stress in the surface of the weldment. Additionally, the influence of rolling parameters on the through-thickness residual stress variation was investigated. Low loads only influence the residual stress near the surface, while high loads affected the material through the entire thickness. When the dual flat roller was used to roll next to the weld bead, significant compressive residual stress was induce in the weld bead; however, the residual stress reduction was very sensitive to the contact of the roller to the weld toes; therefore, when rolling a weld bead that varies in shape along the weld, the residual stress reduction is not uniform and varies along the length. On the other hand, the in situ rolling did not produced significant residual stress or distortion reduction in all the cases analysed. The rolling occurred when the material was still hot and the residual stress was subsequently formed as the material cooled to room temperature. Numerical modelling was a very useful tool for understanding the development of stress and plastic strain during the welding and rolling processes

Application of Near Infrared Reflectance Spectroscopy (NIRS) for Macronutrients Analysis in Alfalfa (\u3ci\u3eMedicago sativa\u3c/i\u3e L.)

Near infrared reflectance spectroscopy was used to assess the mineral composition of alfalfa (Medicago sativa L.) as a tool for nutritional diagnosis. One hundred and ninety four (n = 194) samples of alfalfa from different locations representing a wide range of soils were used. Samples were reflectance scanned in a NIRS 6500 (NIRSystems, USA) instrument. The coefficients of determination (R2) of the regression estimate of the concentration of nitrogen, calcium, phosphorus, potassium, magnesium and sulphur and the errors in cross validation (SECV) were 0.93 (SECV: 1.6), 0.95 (SECV: 1.3), 0.93 (SECV: 1.9), 0.88 (SECV: 2.8), 0.82 (SECV: 1.9) and 0.75 (SECV: 4.7) respectively. The best NIRS predictions were obtained for calcium and nitrogen, meanwhile the poorest was obtained for sulphur

Effects of ocean acidification on invertebrate settlement at volcanic CO<inf>2</inf> vents

We present the first study of the effects of ocean acidification on settlement of benthic invertebrates and microfauna. Artificial collectors were placed for 1 month along pH gradients at CO2 vents off Ischia (Tyrrhenian Sea, Italy). Seventy-nine taxa were identified from six main taxonomic groups (foraminiferans, nematodes, polychaetes, molluscs, crustaceans and chaetognaths). Calcareous foraminiferans, serpulid polychaetes, gastropods and bivalves showed highly significant reductions in recruitment to the collectors as pCO2 rose from normal (336-341 ppm, pH 8.09-8.15) to high levels (886-5,148 ppm) causing acidified conditions near the vents (pH 7.08-7.79). Only the syllid polychaete Syllis prolifera had higher abundances at the most acidified station, although a wide range of polychaetes and small crustaceans was able to settle and survive under these conditions. A few taxa (Amphiglena mediterranea, Leptochelia dubia, Caprella acanthifera) were particularly abundant at stations acidified by intermediate amounts of CO2 (pH 7. 41-7.99). These results show that increased levels of CO2 can profoundly affect the settlement of a wide range of benthic organisms. © 2010 Springer-Verlag

Screening of antioxidant properties of the apple juice using the front-face synchronous fluorescence and chemometrics

Fluorescence spectroscopy is gaining increasing attention in food analysis due to its higher sensitivity and selectivity as compared to other spectroscopic techniques. Synchronous scanning fluorescence technique is particularly useful in studies of multi-fluorophoric food samples, providing a further improvement of selectivity by reduction in the spectral overlapping and suppressing light-scattering interferences. Presently, we study the feasibility of the prediction of the total phenolics, flavonoids, and antioxidant capacity using front-face synchronous fluorescence spectra of apple juices. Commercial apple juices from different product ranges were studied. Principal component analysis (PCA) applied to the unfolded synchronous fluorescence spectra was used to compare the fluorescence of the entire sample set. The regression analysis was performed using partial least squares (PLS1 and PLS2) methods on the unfolded total synchronous and on the single-offset synchronous fluorescence spectra. The best calibration models for all of the studied parameters were obtained using the PLS1 method for the single-offset synchronous spectra. The models for the prediction of the total flavonoid content had the best performance; the optimal model was obtained for the analysis of the synchronous fluorescence spectra at Delta lambda = 110 nm (R (2) = 0.870, residual predictive deviation (RPD) = 2.7). The optimal calibration models for the prediction of the total phenolic content (Delta lambda = 80 nm, R (2) = 0.766, RPD = 2.0) and the total antioxidant capacity (Delta lambda = 70 nm, R (2) = 0.787, RPD = 2.1) had only an approximate predictive ability. These results demonstrate that synchronous fluorescence could be a useful tool in fast semi-quantitative screening for the antioxidant properties of the apple juices.info:eu-repo/semantics/publishedVersio

Predicción de fracciones fibrosas, cenizas y digestibilidad de la materia orgánica en rastrojo de maíz mediante el uso de la reflectancia en el infrarrojo cercano

Near infrared reflectance (NIR) spectroscopy was used to predict acid and neutral detergent fibre (ADF and NDF), ash, and organic matter digestibility (OMD) in maize stover samples. Samples were analyzed by reference laboratory methods and spectra collected using a NIR spectrophotometer in reflectance (1100 – 2500 nm). Predictive equations were developed using partial least squares (PLS) with full cross validation using second derivative. The coefficient of determination in calibration (R2 cal) and the standard error in cross validation (SECV) were for ADF 0.85 (SECV: 16.5 g kg-1 DM), for NDF 0.92 (SECV: 58.4 g kg-1 DM), for ash 0.90 (SECV: 1.9 g kg-1 DM) and for OMD 0.85 (SECV: 10.4 g kg-1 DM). The results from this study suggested that maize stover samples might be analysed by NIR spectroscopy in order to determine ADF, NDF and ash. However, the prediction accuracy is less than desirable to be used for routine analytical purposes.El uso de la reflectancia en el infrarrojo cercano (NIR) fue evaluada para la determinación de fibra detergente neutro (FDN), fibra detergente ácida (FDA), cenizas y digestibilidad de la materia orgánica (DMO) en rastrojo de maíz.&nbsp; &nbsp;Las muestras se analizaron usando métodos de referencia y los espectros se obtuvieron usando un instrumento NIR (1100 – 2500 nm). &nbsp;Las ecuaciones de predicción fueron desarrolladas usando el método de mínimos cuadrados (PLS), validación cruzada y segunda&nbsp; derivada. &nbsp;Los coeficientes de determinación (R2cal) en calibración y el error standard de la validación cruzada (SECV) fueron para FDA 0.85 (SECV: 16.5 g kg-1 DM), para FDN 0.92 (SECV: 58.4 g kg-1 DM), para cenizas 0.90 (SECV: 1.9 g kg-1 DM) y para DMO 0.85 (SECV: 10.4 g kg-1&nbsp;DM). &nbsp;Los resultados sugieren que la técnica NIRS puede determinar FDA, FDN, y cenizas. &nbsp;Sin embargo la precisión no es buena para ser usada en análisis de rutina