Biofilm development by clinical strains of non-pigmented rapidly growing mycobacteria

AbstractThe relationship between clinical significance of non-pigmented, rapidly growing mycobacteria (NPRGM), in vitro biofilm development and sliding motility was evaluated in this study. One hundred and sixty-eight clinical strains of NPRGM were included. Forty-one of these were clinically significant isolates. Biofilm was formed by 123 strains. Seventy-six biofilm-positive and 25 biofilm-negative strains showed sliding motility. There was a relationship between clinical significance and biofilm development (p <0.000 001), sliding motility (p 0.0037) and species (p <0.000 001). No relationship was found between motility and biofilm development. The ability to develop biofilm is a characteristic that can have importance in the development of infections caused by NPRGM

Genomic transformation and social organization during the Copper Age-Bronze Age transition in southern Iberia

The emerging Bronze Age (BA) of southeastern Iberia saw marked social changes. Late Copper Age (CA) settlements were abandoned in favor of hilltop sites, and collective graves were largely replaced by single or double burials with often distinctive grave goods indirectly reflecting a hierarchical social organization, as exemplified by the BA El Argar group. We explored this transition from a genomic viewpoint by tripling the amount of data available for this period. Concomitant with the rise of El Argar starting ~2200 cal BCE, we observe a complete turnover of Y-chromosome lineages along with the arrival of steppe-related ancestry. This pattern is consistent with a founder effect in male lineages, supported by our finding that males shared more relatives at sites than females. However, simple two-source models do not find support in some El Argar groups, suggesting additional genetic contributions from the Mediterranean that could predate the BA

Factor analysis of the Zung self-rating depression scale in a large sample of patients with major depressive disorder in primary care

<p>Abstract</p> <p>Background</p> <p>The aim of this study was to examine the symptomatic dimensions of depression in a large sample of patients with major depressive disorder (MDD) in the primary care (PC) setting by means of a factor analysis of the Zung self-rating depression scale (ZSDS).</p> <p>Methods</p> <p>A factor analysis was performed, based on the polychoric correlations matrix, between ZSDS items using promax oblique rotation in 1049 PC patients with a diagnosis of MDD (DSM-IV).</p> <p>Results</p> <p>A clinical interpretable four-factor solution consisting of a <it>core depressive </it>factor (I); a <it>cognitive </it>factor (II); an <it>anxiety </it>factor (III) and a <it>somatic </it>factor (IV) was extracted. These factors accounted for 36.9% of the variance on the ZSDS. The 4-factor structure was validated and high coefficients of congruence were obtained (0.98, 0.95, 0.92 and 0.87 for factors I, II, III and IV, respectively). The model seemed to fit the data well with fit indexes within recommended ranges (GFI = 0.9330, AGFI = 0.9112 and RMR = 0.0843).</p> <p>Conclusion</p> <p>Our findings suggest that depressive symptoms in patients with MDD in the PC setting cluster into four dimensions: <it>core depressive, cognitive, anxiety </it>and <it>somatic</it>, by means of a factor analysis of the ZSDS. Further research is needed to identify possible diagnostic, therapeutic or prognostic implications of the different depressive symptomatic profiles.</p

Abordando la exposición a las emisiones del tabaco y de los cigarrillos electrónicos: protocolo del proyecto TackSHS

Objective: The TackSHS project aims to comprehensively elucidate the impact that exposure to second-hand smoke (SHS) from cigarettes and second-hand aerosols (SHA) from electronic cigarettes have on the respiratory health of the European population according to socioeconomic characteristics and other determinants. Method: The TackSHS project involves a series of coordinated studies carried out by 11 academic and public health organisations from six European countries. The project will investigate: a) the determinants of SHS and SHA exposure assessed at the individual level (surveys on representative general population samples) and in common environments (environmental sampling in specific settings); b) the overall disease burden, mortality and morbidity attributable to such exposure; and c) its economic impact in terms of direct health care costs. The project will also examine specific acute respiratory health changes in healthy individuals and patients with respiratory diseases exposed to SHS and SHA. In addition, the project will examine the effectiveness of a novel intervention to reduce SHS exposure in households where smoking is permitted. All these studies are inter-related and involve collaborative coordination among the participant organisations. Conclusion: The comprehensive, integrated approach of the TackSHS project will enable a significant step forward from the current status quo in the understanding of the impact of SHS and SHA exposure on health and provide the basis for health policy recommendations to help European countries to further reduce the harm caused by SHS and SHA exposure

A multivariant secure framework for smart mobile health application

This is an accepted manuscript of an article published by Wiley in Transactions on Emerging Telecommunications Technologies, available online: https://doi.org/10.1002/ett.3684 The accepted version of the publication may differ from the final published version.Wireless sensor network enables remote connectivity of technological devices such as smart mobile with the internet. Due to its low cost as well as easy availability of data sharing and accessing devices, the Internet of Things (IoT) has grown exponentially during the past few years. The availability of these devices plays a remarkable role in the new era of mHealth. In mHealth, the sensors generate enormous amounts of data and the context-aware computing has proven to collect and manage the data. The context aware computing is a new domain to be aware of context of involved devices. The context-aware computing is playing a very significant part in the development of smart mobile health applications to monitor the health of patients more efficiently. Security is one of the key challenges in IoT-based mHealth application development. The wireless nature of IoT devices motivates attackers to attack on application; these vulnerable attacks can be denial of service attack, sinkhole attack, and select forwarding attack. These attacks lead intruders to disrupt the application's functionality, data packet drops to malicious end and changes the route of data and forwards the data packet to other location. There is a need to timely detect and prevent these threats in mobile health applications. Existing work includes many security frameworks to secure the mobile health applications but all have some drawbacks. This paper presents existing frameworks, the impact of threats on applications, on information, and different security levels. From this line of research, we propose a security framework with two algorithms, ie, (i) patient priority autonomous call and (ii) location distance based switch, for mobile health applications and make a comparative analysis of the proposed framework with the existing ones.Published onlin

Causes, consequences and biomarkers of stress in swine: an update

BACKGROUND: In recent decades there has been a growing concern about animal stress on intensive pig farms due to the undesirable consequences that stress produces in the normal physiology of pigs and its effects on their welfare and general productive performance. This review analyses the most important types of stress (social, environmental, metabolic, immunological and due to human handling), and their biological consequences for pigs. The physio-pathological changes associated with stress are described, as well as the negative effects of stress on pig production. In addition an update of the different biomarkers used for the evaluation of stress is provided. These biomarkers can be classified into four groups according to the physiological system or axis evaluated: sympathetic nervous system, hypothalamic-pituitary-adrenal axis, hypothalamic-pituitary-gonadal axis and immune system. CONCLUSIONS: Stress it is a process with multifactorial causes and produces an organic response that generates negative effects on animal health and production. Ideally, a panel of various biomarkers should be used to assess and evaluate the stress resulting from diverse causes and the different physiological systems involved in the stress response. We hope that this review will increase the understanding of the stress process, contribute to a better control and reduction of potential stressful stimuli in pigs and, finally, encourage future studies and developments to better monitor, detect and manage stress on pig farms

Use of Posidonia oceanica seedlings from beach-cast fruits for seagrass planting

The final publication is available at www.degruyter.comPosidonia oceanica seedlings produced from beach-cast fruits were planted in an area where this Mediterranean seagrass was lost as a consequence of fish-farming. The effects of substratum type (dead matte vs. meadow) and planting level (above vs. below bottom surface) on seedling survivorship and leaf development were evaluated after 3 years. Seedling capacity to resist uprooting was also tested by comparing survivorship and leaf development after 2 years in seedlings firmly anchored in dead matte by artificial means (mesh-pot) with those planted without any anchoring other than their roots. Survivorship in dead matte was 44% after 3 years and was not affected by planting level. Seedling planting inside the meadow led to complete mortality after 3 years. Seedlings planted below the bottom surface branched during the second year and 50% had produced from one to five additional shoots after 3 years. After 2 years, survivorship and leaf development of seedlings planted with mesh-pot anchoring was similar to that of seedlings planted without anchoring. Current environmental conditions in the impacted area allow P. oceanica seedlings to survive, but natural recovery might be limited by propagule availability. Planting of seedlings in dead matte without anchoring does not affect survivorship and leaf development and might be a feasible technique for restoration of P. oceanica meadows.Funds were provided by research grant 116/SGTB/2007/1.3 of Ministerio de Medio Ambiente.Peer reviewe

Experimental Evaluation of the Restoration Capacity of a Fish-Farm Impacted Area with Posidonia oceanica (L.) Delile Seedlings

Marine aquaculture is an activity that has induced severe local losses of seagrass meadows along the coastal areas. The purpose of this study was to evaluate the capacity of an area degraded by fish-farm activities to support Posidonia oceanica seedlings. In the study site, a bay in the southeast coast of Spain where part of a meadow disappeared by fish-farm activities, seedlings inside mesh-pots were planted in three areas. Two plots were established in each area, one in P. oceanica dead matte and another inside a P. oceanica meadow. To evaluate if sediment conditions were adequate for the life of the seedlings, half of them were planted in direct contact with the sediment and the other half were planted above the surface of the sediment in each plot. Monitoring during 1 year showed that there were large differences in seedling survival between the dead matte and the P. oceanica meadow. While seedlings planted in dead matte had a high survivorship after 1 year (75%), seedlings planted in P. oceanica progressively died (survivorship of 20% after 1 year). The average leaf length of the seedlings surviving in the two substrata was not different, but the leaf area per seedling was lower in the seedlings growing inside the P. oceanica meadow during most part of the year. Seedling survivorship and vegetative development were not affected by the level of planting and suggest that the sediment conditions are adequate for the life of P. oceanica seedlings. © 2011 Society for Ecological Restoration International.Funds for this study were provided by the research grant 116/SGTB/2007/1.3 of the Ministerio de Medio Ambiente, Rural y Marino and the Ministerio de Ciencia e Innovación Español, Programa Nacional de Formación de Profesorado Universitario of SpainPeer Reviewe