Effect of light and sucrose on photoautotrophic and photomixotrophic micropropagation of Physalis angulata

The biotechnological interest in genus Physalis has increased in the last decades, however, there are still few micropropagation studies of this genus. The objective of this study was to evaluate P. angulata photoautotrophic and photomixotrophic micropropagation with gas exchange under seven light spectra and five concentrations of sucrose. Lighting were yellow, blue, white, red, green, red + blue LEDs and natural light filtered by mesh. Sucrose concentrations were 0, 7.5, 15, 22.5 and 30 g.L-1. Phytotechnical, anatomical features and photopigment contents were evaluated through stem and root segment length, leaf number, leaf area, chlorophyll a and b contents, carotenoids, adaxial epidermis, palisadic and spongy parenchyma and abaxial epidermis. The data were compared by Scott-Knott's mean test and principal components analysis using the R software. Comparing the variables within lighting types, it was observed that only the screen treatment, screen-filtered natural illumination, obtained assessment in all variables. Comparing the levels of sucrose, it was observed that the treatment 15 g.L-1 sucrose obtained the highest number of averages with maximum evaluation. It was concluded that the natural light filtered by screen with 50% of shading allowed the photoautotrophic micropropagation of P. angulata. Better development results were observed in photomixotrophic micropropagation with 15 g.L-1 of sucrose

Projeto Carroceiro FZEA-USP

The Cart Horse Project, created and organized by professor Renata Gebara Sampaio Dória (DVM, MSc, PhD), started its activities in 2011. Since then, it has been contributing to several families from Pirassununga and nearby cities that cannot afford veterinary care to their animals. The project aims to provide substantial knowledge and awareness on zoonosis, public health, handling and animal welfare and health of the owners of traction horses used as a worker for these families. Besides promoting health and welfare of these animals, as well as raising awareness on the owners, the project also benefits the Veterinary Medicine students of Faculty of Animal Husbandry and Food Engineering of University of São Paulo (FZEA-USP) in order to develop and improve their clinical and surgical abilities on equine medicine, complementing their professional education.O projeto Carroceiro, coordenado e idealizado pela Profa. Dra. Renata Gebara Sampaio Dória, iniciou suas atividades no ano de 2011 e, desde então, vem contribuindo com muitas famílias de Pirassununga e região que utilizam equídeos para tração e não possuem condições financeiras para fornecer a seus animais uma assistência médica veterinária. O trabalho objetiva a conscientização dos proprietários dos equídeos utilizados em carroças (carroceiros) sobre: zoonoses, saúde pública, manejo, bem-estar e promoção da saúde desses animais, que são utilizados para tração como fonte de subsistência por muitas famílias. Além de promover a saúde e bem-estar dos animais, bem como a conscientização dos carroceiros, o projeto auxilia os estudantes de Medicina Veterinária da Faculdade de Zootecnia e Engenharia de Alimentos da Universidade de São Paulo (FZEA-USP) a desenvolver e aprimorar suas habilidades clínico-cirúrgicas no tratamento de equídeos, complementando a formação profissional

Polyploidy induction in Physalis alkekengi

Physalis alkekengi is an ornamental plant that can also be used as a medicinal plant due to its anti-inflammatory, bactericidal, antitumor and fungicidal properties. Polyploidization can be an important tool in the genetic improvement of this species. The objective this work was to obtain tetraploids in vitro and to evaluate the phytotechnical traits of P. alkekengi. For this, nodal segments of P. alkekengi var. Franchettii were inoculated into petri dishes containing 100 ml of MS medium supplemented with colchicine at concentrations 0; 0.04; 0.08; 0.12; and 0.16% and kept in the dark for 24 and 48h. After the respective treatment periods with colchicine the segments were inoculated into test tubes. The tetraploids were identified by flow cytometry and classical cytogenetics. In vitro seedlings were measured: root length, nodal segment length, leaflet number and total leaf area. In the acclimatization phase, the area of ​​the second leaf and total leaf, petiole radius, stem length, fruit weight with calyx, without calyx, fruit diameter, number of seeds and brix of the pulp were evaluated. Chlorophyll a, chlorophyll b, total chlorophyll, total carotenoid, total chlorophyll / total carotenoid ratio and chlorophyll a / b ratio were also estimated. The treatment that most produced tetraploid seedlings was with 0.08% colchicine per 24h. No significant difference was observed in 7 (seven) variables, these being all variables of photopigments, stem diameter (steam) and brix. In general, diploid (2x) plants were better in 9 (nine) while tetraploid seedlings were better in 6 (six) of the phytotechnical variables. It was concluded that the MS medium supplemented with 0.08% colchicine for 24 h allowed P. alkekengi tetraploides to be obtained with better phytotechnical qualities

Fatores de risco associados ao desenvolvimento de Câncer de Ovário: revisão integrativa

Introdução: O câncer de ovário (CO) é o nono tipo de câncer mais incidente entre as mulheres no mundo e a segunda neoplasia ginecológica mais comum no Brasil. Objetivo: Descrever por meio da revisão de literatura, os fatores de risco associados ao desenvolvimento de câncer de ovário em mulheres. Metodologia: Trata-se de uma revisão integrativa. A busca bibliográfica ocorreu nas bases: Biblioteca Virtual de Saúde (BVS), Scientific Electronic Library Online (SciELO) e Public Medline (PubMed). Descritores foram utilizados para as buscas, usando os operadores AND e OR. Os critérios de inclusão foram: publicações de qualquer período, disponíveis na íntegra e gratuitamente, nos idiomas português, inglês ou espanhol. Resultados: Evidenciou-se diversos fatores de riscos associados ao CO. O tabagismo e o consumo excessivo de café foram identificados como fatores de risco significativos. Por outro lado, o consumo moderado de vinho, especialmente o tinto pode ter um efeito protetor contra o câncer. A predisposição genética, como a presença de mutações BRCA1 e BRCA2, história clínica familiar, menopausa e idade avançada, além do sedentarismo, especialmente em ambientes de trabalho, e a obesidade foram preditores do desfecho estudado. Considerações finais: Faz-se importante o direcionamento de estratégias de prevenção, como campanhas educativas sobre os riscos do sedentarismo, importância de uma dieta equilibrada, realização de exames periódicos, entre outros

Nationwide access to endovascular treatment for acute ischemic stroke in portugal

Publisher Copyright: Copyright Ordem dos M dicos 2021.Introduction: Since the publication of endovascular treatment trials and European Stroke Guidelines, Portugal has re-organized stroke healthcare. The nine centers performing endovascular treatment are not equally distributed within the country, which may lead to differential access to endovascular treatment. Our main aim was to perform a descriptive analysis of the main treatment metrics regarding endovascular treatment in mainland Portugal and its administrative districts. Material and Methods: A retrospective national multicentric cohort study was conducted, including all ischemic stroke patients treated with endovascular treatment in mainland Portugal over two years (July 2015 to June 2017). All endovascular treatment centers contributed to an anonymized database. Demographic, stroke-related and procedure-related variables were collected. Crude endovascular treatment rates were calculated per 100 000 inhabitants for mainland Portugal, and each district and endovascular treatment standardized ratios (indirect age-sex standardization) were also calculated. Patient time metrics were computed as the median time between stroke onset, first-door, and puncture. Results: A total of 1625 endovascular treatment procedures were registered. The endovascular treatment rate was 8.27/100 000 inhabitants/year. We found regional heterogeneity in endovascular treatment rates (1.58 to 16.53/100 000/year), with higher rates in districts closer to endovascular treatment centers. When analyzed by district, the median time from stroke onset to puncture ranged from 212 to 432 minutes, reflecting regional heterogeneity. Discussion: Overall endovascular treatment rates and procedural times in Portugal are comparable to other international registries. We found geographic heterogeneity, with lower endovascular treatment rates and longer onset-to-puncture time in southern and inner regions. Conclusion: The overall national rate of EVT in the first two years after the organization of EVT-capable centers is one of the highest among European countries, however, significant regional disparities were documented. Moreover, stroke-onset-to-first-door times and in-hospital procedural times in the EVT centers were comparable to those reported in the randomized controlled trials performed in high-volume tertiary hospitalspublishersversionpublishe

Acesso a Tratamento Endovascular para Acidente Vascular Cerebral Isquémico em Portugal

Introduction: Since the publication of endovascular treatment trials and European Stroke Guidelines, Portugal has re-organized stroke healthcare. The nine centers performing endovascular treatment are not equally distributed within the country, which may lead to differential access to endovascular treatment. Our main aim was to perform a descriptive analysis of the main treatment metrics regarding endovascular treatment in mainland Portugal and its administrative districts. Material and Methods: A retrospective national multicentric cohort study was conducted, including all ischemic stroke patients treated with endovascular treatment in mainland Portugal over two years (July 2015 to June 2017). All endovascular treatment centers contributed to an anonymized database. Demographic, stroke-related and procedure-related variables were collected. Crude endovascular treatment rates were calculated per 100 000 inhabitants for mainland Portugal, and each district and endovascular treatment standardized ratios (indirect age-sex standardization) were also calculated. Patient time metrics were computed as the median time between stroke onset, first-door, and puncture. Results: A total of 1625 endovascular treatment procedures were registered. The endovascular treatment rate was 8.27/100 000 inhabitants/year. We found regional heterogeneity in endovascular treatment rates (1.58 to 16.53/100 000/year), with higher rates in districts closer to endovascular treatment centers. When analyzed by district, the median time from stroke onset to puncture ranged from 212 to 432 minutes, reflecting regional heterogeneity. Conclusion: The overall national rate of EVT in the first two years after the organization of EVT-capable centers is one of the highest among European countries, however, significant regional disparities were documented. Moreover, stroke-onset-to-first-door times and in-hospital procedural times in the EVT centers were comparable to those reported in the randomized controlled trials performed in high-volume tertiary hospitals.Introdução: A aprovação do tratamento endovascular para o acidente vascular cerebral isquémico obrigou à reorganização dos cuidados de saúde em Portugal. Os nove centros que realizam tratamento endovascular não estão distribuídos equitativamente pelo território, o que poderá causar acesso diferencial a tratamento. O principal objetivo deste estudo é realizar uma análise descritiva da frequência e métricas temporais do tratamento endovascular em Portugal continental e seus distritos. Material e Métodos: Estudo de coorte nacional multicêntrico, incluindo todos os doentes com acidente vascular cerebral isquémico submetidos a tratamento endovascular em Portugal continental durante um período de dois anos (julho 2015 a junho 2017). Foram colhidos dados demográficos, relacionados com o acidente vascular cerebral e variáveis do procedimento. Taxas de tratamento endovascular brutas e ajustadas (ajuste indireto a idade e sexo) foram calculadas por 100 000 habitantes/ano para Portugal continental e cada distrito. Métricas de procedimento como tempo entre instalação, primeira porta e punção foram também analisadas. Resultados: Foram registados 1625 tratamentos endovasculares, indicando uma taxa bruta nacional de tratamento endovascular de 8,27/100 000 habitantes/ano. As taxas de tratamento endovascular entre distritos variaram entre 1,58 e 16,53/100 000/ano, com taxas mais elevadas nos distritos próximos a hospitais com tratamento endovascular. O tempo entre sintomas e punção femural entre distritos variou entre 212 e 432 minutos. Conclusão: Portugal continental apresenta uma taxa nacional de tratamento endovascular elevada, apresentando, contudo, assimetrias regionais no acesso. As métricas temporais foram comparáveis com as observadas nos ensaios clínicos piloto

Avaliação dos transtornos psiquiátricos em adolescentes em conflito com a lei

Orientador: Prof. Dr. Sergio Antonio AntoniukCo-orientador: Prof. Dr. Francisco B. Assumpção Jr.Tese (doutorado) - Universidade Federal do Paraná, Setor de Ciências da Saúde, Programa de Pós-Graduação em Saúde da Criança e do Adolescente. Defesa: Curitiba, 19/12/2011Inclui referênciasResumo: Observa-se um crescente número de jovens envolvidos com problemas com a lei no Brasil e também em países com níveis socioeconômicos mais estáveis e com menor desigualdade social. O problema dos transtornos psiquiátricos nessa população é uma realidade, já confirmada nos poucos estudos realizados e, portanto, estudos com essa população são necessários para se conhecer a melhor forma de se utilizar os escassos recursos disponíveis para a saúde mental. Este estudo teve como objetivo pesquisar a prevalência de transtornos psiquiátricos na população de adolescentes em conflito com a lei. Para estabelecer o diagnóstico, utilizou-se a Entrevista Diagnóstica Kiddie-Sads referente ao momento presente e ao histórico de vida (K-SADS-PL), a Versão Brasileira da Schedule for Affective Disorders and Schizophrenia for School Aged-Children para crianças e adolescentes de 6 a 18 anos, com 69 adolescentes do sexo masculino, brancos, afrodescendentes, pardos e amarelos, com idades de 12 a 16 anos e 11 meses. Foram selecionados adolescentes que estavam cumprindo medida socioeducativa, em regime de internamento, de 45 dias na Vara de Adolescentes Infratores, na cidade de Curitiba, PR. O perfil do adolescente da pesquisa foi de uma idade média de 15,5 anos, pertencente às classes sociais menos favorecidas (87,0%). Os adolescentes viviam em bairros da periferia de Curitiba ou municípios da região metropolitana, locais reconhecidos pelos altos índices de violência. A baixa escolaridade foi a regra: 82,5% não haviam terminado o ensino médio, sendo que 43,4% não terminaram a 5º série e 73,9% não estavam frequentando a escola. Os adolescentes são filhos de mães solteiras e pais separados em sua maioria, com pais com baixa escolaridade e com transtornos psiquiátricos. Também tinham parentes de primeiro grau envolvidos com problemas com a lei em uma incidência significativa. Com o instrumento K-SADS-PL objetivou-se estimar a prevalência de transtornos psiquiátricos, nos últimos seis meses (ao momento presente). Estudos populacionais de transtornos psiquiátricos na população de crianças e adolescentes tendem a apontar uma incidência entre 12 e 20%. Os resultados obtidos na população dos adolescentes em conflito com a lei foram de uma substancial prevalência de transtornos psiquiátricos (81,1%). O transtorno mais prevalente foi o Transtorno de Conduta (59,4%), seguido do Abuso de Substâncias (53,6%), Transtorno de Déficit de Atenção/Hiperatividade (43,5%), Transtorno de Ansiedade (24,6%), Transtorno de Humor (15,9), Enurese Noturna (2,9%) e Transtorno de Tourette (1,4%). Também se verificou que esses jovens apresentavam comorbidades psiquiátricas, 17,4% com dois transtornos psiquiátricos, 26,1% com três transtornos, 14,5% com quatro e 1,4% com cinco transtornos. Estes resultados sugerem uma substancial morbidade psiquiátrica entre os jovens detidos. Sem cuidados adequados na área da saúde mental, uma grande parte desses jovens se tornará uma população adulta problemática, gerando sérios prejuízos individuais, familiares e sociais. Palavras-chave: Adolescente em Conflito com a Lei; Avaliação de Riscos; Transtornos Psiquiátricos; Fatores de Risco.Abstract: We observe a growing number of young people involved in trouble with the law both in Brazil and in countries with more stable socioeconomic levels and less social inequality. The problem of psychiatric disorders among this population is a reality, already confirmed by the few studies realized and, therefore, we are in need of studies of this population to get to know how to employ the meager resources available for mental health care in the most effective ways. This study tried to analyze the prevalence of psychiatric disorders in the population of adolescents in conflict with the law. To establish the diagnosis, the Kiddie-Sads Diagnostic Interview referent to the Present and the Lifetime (K-SADS-PL) was employed, as well as the Brazilian version for the Schedule for Affective Disorders and Schizophrenia for School Aged-Children for kids from 6 to 18 years old, in interviews with 69 male teenagers, both white, African-Brazilian, mulatto and Oriental-Brazilian, with ages ranging from 12 to 16 years and 11 months. We randomly selected teenagers who were on socio-educational reclusion, for 45 days each, in the Teenage Misdemeanors Court, in the city of Curitiba, Paraná. The profile of the researched adolescents revealed an average age of 15.5 years and that they belonged to the less favored strata of the society (87%). The teenagers lived in the outskirts of the city or in small towns in the metropolitan region, places known for their high violence rates. Few years of school were the rule. with 82.5% not having finished high school, of which 43.4% had not a full 5th grade and 73.9% not being at school at all. The teenagers in their majority are the sons of single mothers and separated parents, with parents with low schooling and a history of psychiatric disorders. They also had a significant number of first degree relatives involved in problems with the law. With the K-SADS-PL tool we tried to evaluate the prevalence of psychiatric disorders in the last six months (to the present moment). Demographic studies of psychiatric disorders in the population of children and adolescents point to something between 12 and 20%. The results obtained in the population of adolescents in conflict with the law revealed a meaningful prevalence of psychiatric disorders (81.1%). The most prevalent disorder was Conduct Disorder (59.43%), followed by Substance Abuse (53.6%), Attention Deficit Disorder/Hyperactivity (43.5%), Anxiety Disorder (24.6%), Mood Disorder (15.9%), nocturnal enuresis (2.9%) and Tourette’s Disorder (1.4%). We found also that these kids showed psychiatric comorbidities: 17.4% with two psychiatric disorders, 26.1% with three, 14.5% with four and 1.4% with five disorders. These results suggest a meaningful psychiatric morbidity among the young detained. With no adequate mental health care, most of these kids will become a problematic adult population, with grave damages to the individuals, the families, and the society. Keyword: Adolescent in Conflict with the Law; Risk Assessment; Psychiatric Disorders; Risk factors