MÉTODO DE VALIDAÇÃO E SEPARAÇÃO DE ISOFLAVONAS PRESENTES EM MELAÇO DE SOJA

As isoflavonas atuam no organismo humano com efeitos fisiológicos de forma benéfica tornando os alimentos que contém isoflavonas em formas funcionais ao organismo. Assim, neste trabalho foi desenvolvido um método de análise por Cromatografia Líquida de Alta Eficiência (CLAE) bem como, separação e quantificação de isoflavonas presentes no melaço de soja. A validação do método foi baseada pela linearidade, limite de detecção (LD) e limite de quantificação (LQ) com estabelecimentos de critérios de análise para aceitação da metodologia proposta. Os resultados obtidos na separação dos isômeros de isoflavonas bem como a quantificação trouxeram melhorias relacionadas ao tempo de retenção de cada isômero estudado e separação dos compostos. Com relação ao método aplicado ao longo do estudo este apresentou resultados pertinentes para utilização e expansão do método proposto focado nos compostos de isoflavonas formas glicosídicas e agliconas presentes no melaço de soja. ABSTRACTIsoflavones act in the human body with physiological effects in a beneficial way making foods containing isoflavones in functional forms to the body. Thus, in this work a high efficiency liquid chromatography (HPLC) analysis method was developed, as well as, separation and quantification of isoflavones from in soybean molasses. The validation of the method was based on linearity, limit of detection (LD) and limit of quantification (LQ) with establishments of analysis criteria for acceptance of the proposed methodology. The results obtained in the separation of the isoflavone isomers as well as the quantification brought improvements related to the retention time of each studied isomer and separation of the compounds. In relation to the method applied throughout the study, it presented relevant results for the use and expansion of the proposed method focused on the isoflavone compounds glycosidic forms and aglycones from soybean molasses

Activated charcoal from green coconut as an alternative to remove 2,4D from water and reduce toxicity in Lactuca sativa L

Water quality is essential for the maintenance of all forms of life on the planet, since the  consumption of contaminated water can pose health risks. In this study, green coconut activated charcoal was used in the treatment of contaminated water at concentrations of 2, 5, 10 and 20 mg/L of the herbicide dichlorophenoxyacetic acid (2,4-D). In order to verify the efficiency of the adsorption process, germination bioassays and cytogenetic analyses were performed with seeds of Lactuca sativa L. as a bioindicator. The germination bioassays were carried out with a germination paper roll in triplicate, with 300 seeds per treatment. As for the cytogenetic analysis, 3000 cells were analyzed per treatment. The results showed that the green coconut activated charcoal has adsorptive potential to remove 2,4-D from water, with germination results of 89.6% for treated water, 92% for pure water, and 0% for contaminated water. Regarding the cytogenetic analysis, Mitotic Index (MI) values were high and did not differ statistically for pure and treated water samples. Yet, they were low for contaminated water. As for chromosomal abnormalities, there was a gradual increase depending on the level of exposure to the different 2,4-D concentrations. Thus, we concluded that 2,4-D is toxic to the development of lettuce seeds, and that green coconut activated charcoal was efficient in the adsorptio

Use of low-cost adsorbent functionalized with iron oxide nanoparticles for ivermectin removal

Ivermectin is a commonly used anti-parasitic drug that has been linked to environmental problems. It has been known to cause neurological and reproductive effects on birds, fish, and other wildlife exposed to it. The overuse of ivermectin can lead to a loss of biodiversity, adding to the global environmental crisis. Thus, the present study aimed to evaluate and investigate the adsorption of ivermectin, using Moringa oleifera seed husks functionalized with iron oxide nanoparticles. In view of the kinetic study, the equilibrium occurred in 400 min with an adsorptive capacity of 89.41 mg g-1, the experimental data fit better with the pseudo-first order model and the Langmuir model, obtaining a maximum capacity value of 143.76 mg g-1. Given the values of the thermodynamic parameters, it was determined that the process occurred spontaneously, exothermic and reversible. The material was reused in five cycles, indicating that the material has great potential for removing emerging contaminants

Preparation of a New Adsorbent Material from Agro-Industrial Waste and Comparison with Commercial Adsorbent for Emerging Contaminant Removal

An adsorbent was developed from agro-industrial residues derived from the seed husks of Moringa oleifera Lam., in which the hydrochar process was used as it is a sustainable, low-cost and easy-to-operate process. In comparison, a commercial adsorbent, activated charcoal from babaçu coconut, was used. Both materials were characterized using SEM, FTIR, zeta potential and BET, showing their morphologies, chemical compositions and textural analyses that proved the adsorption capacity of each material. A cost study was also carried out regarding the production of the materials. For both materials, an equilibrium study was carried out using the following contaminants: metformin, diuron, methylene blue and lead. We aimed to study the use of agro-industrial waste as a new adsorbent material, which was shown to have an average removal for all the contaminants tested of around 84.56–99.13%. The new adsorbent developed had many interactions with the studied contaminants, allowing its use on a large scale since its production cost was low. The main objective of this study was thus to compare a commercial activated charcoal with a biosorbent from agro-industrial waste, prepared by the hydrochar method

Wall Latex Paint with Graphene Oxide Incorporation

Graphene has stood out in several areas of research. The objective of the present work was an unprecedented study in the production of a commercial wall latex paint with graphene oxide incorporation. The developed paint was assessed by performance tests according to the Brazilian standards, and the characterization was performed using scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier-transform infrared (FTIR), thermogravimetric analysis (TGA), and contact angle analysis. The results indicated that GO addition led to a better dispersion of the paint compound mixture even at very low concentrations (1% and 2%). For the dry and wet paint coverage performance tests, the results were superior, 2% and 9%, respectively, when compared with the paint without GO addition. All performance tests were in accordance with the requirements of the Brazilian standards. Thus, it was concluded that GO-containing paint could be a promising building material to be used as a paint additive obtaining superior properties