2,241 research outputs found
Observed Limits on Charge Exchange Contributions to the Diffuse X-ray Background
We present a high resolution spectrum of the diffuse X-ray background from
0.1 to 1 keV for a ~1 region of the sky centered at l=90, b=+60 using a
36-pixel array of microcalorimeters flown on a sounding rocket. With an energy
resolution of 11 eV FWHM below 1 keV, the spectrum's observed line ratios help
separate charge exchange contributions originating within the heliosphere from
thermal emission of hot gas in the interstellar medium. The X-ray sensitivity
below 1 keV was reduced by about a factor of four from contamination that
occurred early in the flight, limiting the significance of the results. The
observed centroid of helium-like O VII is 568+2-3 eV at 90% confidence. Since
the centroid expected for thermal emission is 568.4 eV while for charge
exchange is 564.2 eV, thermal emission appears to dominate for this line
complex, consistent with much of the high-latitude O VII emission originating
in 2-3 x 10^6 K gas in the Galactic halo. On the other hand, the observed ratio
of C VI Ly gamma to Ly alpha is 0.3+-0.2. The expected ratios are 0.04 for
thermal emission and 0.24 for charge exchange, indicating that charge exchange
must contribute strongly to this line and therefore potentially to the rest of
the ROSAT R12 band usually associated with 10^6 K emission from the Local Hot
Bubble. The limited statistics of this experiment and systematic uncertainties
due to the contamination require only >32% thermal emission for O VII and >20%
from charge exchange for C VI at the 90% confidence level. An experimental gold
coating on the silicon substrate of the array greatly reduced extraneous
signals induced on nearby pixels from cosmic rays passing through the
substrate, reducing the triggered event rate by a factor of 15 from a previous
flight of the instrument.Comment: 14 pages, 7 figures, to be published in Ap
Fish Cohort Dynamics: Application of Complementary Modeling Approaches
The recruitment to the adult stock of a fish population is a function of both environmental conditions and the dynamics of juvenile fish cohorts. These dynamics can be quite complicated and involve the size structure of the cohort. Two types of models, i-state distribution models (e.g., partial differential equations) and i-state configuration models (computer simulation models following many individuals simultaneously), have been developed to study this type of question. However, these two model types have not to our knowledge previously been compared in detail. Analytical solutions are obtained for three partial differential equation models of early life-history fish cohorts. Equivalent individual-by-individual computer simulation models are also used. These two approaches can produce similar results, which suggests that one may be able to use the approaches interchangeably under many circumstances. Simple uncorrected stochasticity in daily growth is added to the individual-by-individual models, and it is shown that this produces no significant difference from purely deterministic situations. However, when the stochasticity was temporally correlated such that a fish growing faster than the mean 1 d has a tendency to grow faster than the mean the next day, there can be great differences in the outcomes of the simulations.This research was sponsored in part by the Electric Power Research Institute under contract no. RP2932-2 (DOE no. ERD-87-672) with the U.S. Department of Energy under
contract no. DE-AC05-84OR21400 with Martin Marietta Energy Systems, and in part by grant no. NAI6RG0492-01 from the Coastal Ocean Program of the National Oceanic and Atmospheric Administration (NOAA) to the University of North Carolina Sea Grant College Program
Structure and Metal Binding Properties of ZnuA, a Periplasmic Zinc Transporter from \u3cem\u3eEscherichia coli\u3c/em\u3e
ZnuA is the periplasmic Zn2+-binding protein associated with the high-affinity ATP-binding cassette ZnuABC transporter from Escherichia coli. Although several structures of ZnuA and its homologs have been determined, details regarding metal ion stoichiometry, affinity, and specificity as well as the mechanism of metal uptake and transfer remain unclear. The crystal structures of E. coli ZnuA (Eco-ZnuA) in the apo, Zn2+-bound, and Co2+-bound forms have been determined. ZnZnuA binds at least two metal ions. The first, observed previously in other structures, is coordinated tetrahedrally by Glu59, His60, His143, and His207. Replacement of Zn2+ with Co2+ results in almost identical coordination geometry at this site. The second metal binding site involves His224 and several yet to be identified residues from the His-rich loop that is unique to Zn2+ periplasmic metal binding receptors. Electron paramagnetic resonance and X-ray absorption spectroscopic data on CoZnuA provide additional insight into possible residues involved in this second site. The second site is also detected by metal analysis and circular dichroism (CD) titrations. Eco-ZnuA binds Zn2+ (estimated K d \u3c 20 nM), Co2+, Ni2+, Cu2+, Cu+, and Cd2+, but not Mn2+. Finally, conformational changes upon metal binding observed in the crystal structures together with fluorescence and CD data indicate that only Zn2+ substantially stabilizes ZnuA and might facilitate recognition of ZnuB and subsequent metal transfer
Dynactin-dependent cortical dynein and spherical spindle shape correlate temporally with meiotic spindle rotation in Caenorhabditis elegans.
Oocyte meiotic spindles orient with one pole juxtaposed to the cortex to facilitate extrusion of chromosomes into polar bodies. In Caenorhabditis elegans, these acentriolar spindles initially orient parallel to the cortex and then rotate to the perpendicular orientation. To understand the mechanism of spindle rotation, we characterized events that correlated temporally with rotation, including shortening of the spindle in the pole-to pole axis, which resulted in a nearly spherical spindle at rotation. By analyzing large spindles of polyploid C. elegans and a related nematode species, we found that spindle rotation initiated at a defined spherical shape rather than at a defined spindle length. In addition, dynein accumulated on the cortex just before rotation, and microtubules grew from the spindle with plus ends outward during rotation. Dynactin depletion prevented accumulation of dynein on the cortex and prevented spindle rotation independently of effects on spindle shape. These results support a cortical pulling model in which spindle shape might facilitate rotation because a sphere can rotate without deforming the adjacent elastic cytoplasm. We also present evidence that activation of spindle rotation is promoted by dephosphorylation of the basic domain of p150 dynactin
LISA Data Analysis using MCMC methods
The Laser Interferometer Space Antenna (LISA) is expected to simultaneously
detect many thousands of low frequency gravitational wave signals. This
presents a data analysis challenge that is very different to the one
encountered in ground based gravitational wave astronomy. LISA data analysis
requires the identification of individual signals from a data stream containing
an unknown number of overlapping signals. Because of the signal overlaps, a
global fit to all the signals has to be performed in order to avoid biasing the
solution. However, performing such a global fit requires the exploration of an
enormous parameter space with a dimension upwards of 50,000. Markov Chain Monte
Carlo (MCMC) methods offer a very promising solution to the LISA data analysis
problem. MCMC algorithms are able to efficiently explore large parameter
spaces, simultaneously providing parameter estimates, error analyses and even
model selection. Here we present the first application of MCMC methods to
simulated LISA data and demonstrate the great potential of the MCMC approach.
Our implementation uses a generalized F-statistic to evaluate the likelihoods,
and simulated annealing to speed convergence of the Markov chains. As a final
step we super-cool the chains to extract maximum likelihood estimates, and
estimates of the Bayes factors for competing models. We find that the MCMC
approach is able to correctly identify the number of signals present, extract
the source parameters, and return error estimates consistent with Fisher
information matrix predictions.Comment: 14 pages, 7 figure
Evaluation of Surface Modification as a Lunar Dust Mitigation Strategy for Thermal Control Surfaces
Three surface treatments were evaluated for their ability to lower the adhesion between lunar simulant dust and AZ93, AlFEP, and AgFEP thermal control surfaces under simulated lunar conditions. Samples were dusted in situ and exposed to a standardized puff of nitrogen gas. Thermal performance before dusting, after dusting, and after part of the dust was removed by the puff of gas, were compared to perform the assessment. None of the surface treatments was found to significantly affect the adhesion of lunar simulants to AZ93 thermal control paint. Oxygen ion beam texturing also did not lower the adhesion of lunar simulant dust to AlFEP or AgFEP. But a workfunction matching coating and a proprietary Ball Aerospace surface treatment were both found to significantly lower the adhesion of lunar simulants to AlFEP and AgFEP. Based on these results, it is recommended that all these two techniques be further explored as dust mitigation coatings for AlFEP and AgFEP thermal control surfaces
Sodium and Calcium Current-Mediated Pacemaker Neurons and Respiratory Rhythm Generation
The breathing motor pattern in mammals originates in brainstem networks. Whether pacemaker neurons play an obligatory role remains a key unanswered question. We performed whole-cell recordings in the pre-Botzinger complex in slice preparations from neonatal rodents and tested for pacemaker activity. We observed persistent Na+ current (INaP)-mediated bursting in ∼5% of inspiratory neurons in postnatal day 0 (P0)-P5 and in P8-P10 slices. INaP-mediated bursting was voltage dependent and blocked by 20 μm riluzole (RIL). We found Ca2+ current (ICa)-dependent bursting in 7.5% of inspiratory neurons in P8-P10 slices, but in P0-P5 slices these cells were exceedingly rare (0.6%). This bursting was voltage independent and blocked by 100 μm Cd2+ or flufenamic acid (FFA) (10-200 μm), which suggests that a Ca2+-activated inward cationic current (ICAN) underlies burst generation. These data substantiate our observation that P0-P5 slices exposed to RIL contain few (if any) pacemaker neurons, yet maintain respiratory rhythm. We also show that 20 nm TTX or coapplication of 20 μm RIL + FFA (100-200 μm) stops the respiratory rhythm, but that adding 2 μm substance P restarts it. We conclude that INaP and ICAN enhance neuronal excitability and promote rhythmogenesis, even if their magnitude is insufficient to support bursting-pacemaker activity in individual neurons. When INaP and ICAN are removed pharmacologically, the rhythm can be maintained by boosting neural excitability, which is inconsistent with a pacemaker-essential mechanism of respiratory rhythmogenesis by the pre-Botzinger complex
Sensitivity and parameter-estimation precision for alternate LISA configurations
We describe a simple framework to assess the LISA scientific performance
(more specifically, its sensitivity and expected parameter-estimation precision
for prescribed gravitational-wave signals) under the assumption of failure of
one or two inter-spacecraft laser measurements (links) and of one to four
intra-spacecraft laser measurements. We apply the framework to the simple case
of measuring the LISA sensitivity to monochromatic circular binaries, and the
LISA parameter-estimation precision for the gravitational-wave polarization
angle of these systems. Compared to the six-link baseline configuration, the
five-link case is characterized by a small loss in signal-to-noise ratio (SNR)
in the high-frequency section of the LISA band; the four-link case shows a
reduction by a factor of sqrt(2) at low frequencies, and by up to ~2 at high
frequencies. The uncertainty in the estimate of polarization, as computed in
the Fisher-matrix formalism, also worsens when moving from six to five, and
then to four links: this can be explained by the reduced SNR available in those
configurations (except for observations shorter than three months, where five
and six links do better than four even with the same SNR). In addition, we
prove (for generic signals) that the SNR and Fisher matrix are invariant with
respect to the choice of a basis of TDI observables; rather, they depend only
on which inter-spacecraft and intra-spacecraft measurements are available.Comment: 17 pages, 4 EPS figures, IOP style, corrected CQG versio
The Challenges in Gravitational Wave Astronomy for Space-Based Detectors
The Gravitational Wave (GW) universe contains a wealth of sources which, with
the proper treatment, will open up the universe as never before. By observing
massive black hole binaries to high redshifts, we should begin to explore the
formation process of seed black holes and track galactic evolution to the
present day. Observations of extreme mass ratio inspirals will allow us to
explore galactic centers in the local universe, as well as providing tests of
General Relativity and constraining the value of Hubble's constant. The
detection of compact binaries in our own galaxy may allow us to model stellar
evolution in the Milky Way. Finally, the detection of cosmic (super)strings and
a stochastic background would help us to constrain cosmological models.
However, all of this depends on our ability to not only resolve sources and
carry out parameter estimation, but also on our ability to define an optimal
data analysis strategy. In this presentation, I will examine the challenges
that lie ahead in GW astronomy for the ESA L3 Cosmic Vision mission, eLISA.Comment: 12 pages. Plenary presentation to appear in the Proceedings of the
Sant Cugat Forum on Astrophysics, Sant Cugat, April 22-25, 201
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