Follow My Eyes: The Gaze of Politicians Reflexively Captures the Gaze of Ingroup Voters

Studies in human and non-human primates indicate that basic socio-cognitive operations are inherently linked to the power of gaze in capturing reflexively the attention of an observer. Although monkey studies indicate that the automatic tendency to follow the gaze of a conspecific is modulated by the leader-follower social status, evidence for such effects in humans is meager. Here, we used a gaze following paradigm where the directional gaze of right- or left-wing Italian political characters could influence the oculomotor behavior of ingroup or outgroup voters. We show that the gaze of Berlusconi, the right-wing leader currently dominating the Italian political landscape, potentiates and inhibits gaze following behavior in ingroup and outgroup voters, respectively. Importantly, the higher the perceived similarity in personality traits between voters and Berlusconi, the stronger the gaze interference effect. Thus, higher-order social variables such as political leadership and affiliation prepotently affect reflexive shifts of attention

Cortactin Phosphorylated by ERK1/2 Localizes to Sites of Dynamic Actin Regulation and Is Required for Carcinoma Lamellipodia Persistence

Tumor cell motility and invasion is governed by dynamic regulation of the cortical actin cytoskeleton. The actin-binding protein cortactin is commonly upregulated in multiple cancer types and is associated with increased cell migration. Cortactin regulates actin nucleation through the actin related protein (Arp)2/3 complex and stabilizes the cortical actin cytoskeleton. Cortactin is regulated by multiple phosphorylation events, including phosphorylation of S405 and S418 by extracellular regulated kinases (ERK)1/2. ERK1/2 phosphorylation of cortactin has emerged as an important positive regulatory modification, enabling cortactin to bind and activate the Arp2/3 regulator neuronal Wiskott-Aldrich syndrome protein (N-WASp), promoting actin polymerization and enhancing tumor cell movement.In this report we have developed phosphorylation-specific antibodies against phosphorylated cortactin S405 and S418 to analyze the subcellular localization of this cortactin form in tumor cells and patient samples by microscopy. We evaluated the interplay between cortactin S405 and S418 phosphorylation with cortactin tyrosine phosphorylation in regulating cortactin conformational forms by Western blotting. Cortactin is simultaneously phosphorylated at S405/418 and Y421 in tumor cells, and through the use of point mutant constructs we determined that serine and tyrosine phosphorylation events lack any co-dependency. Expression of S405/418 phosphorylation-null constructs impaired carcinoma motility and adhesion, and also inhibited lamellipodia persistence monitored by live cell imaging.Cortactin phosphorylated at S405/418 is localized to sites of dynamic actin assembly in tumor cells. Concurrent phosphorylation of cortactin by ERK1/2 and tyrosine kinases enables cells with the ability to regulate actin dynamics through N-WASp and other effector proteins by synchronizing upstream regulatory pathways, confirming cortactin as an important integration point in actin-based signal transduction. Reduced lamellipodia persistence in cells with S405/418A expression identifies an essential motility-based process reliant on ERK1/2 signaling, providing additional understanding as to how this pathway impacts tumor cell migration

Impact of opioid-free analgesia on pain severity and patient satisfaction after discharge from surgery: multispecialty, prospective cohort study in 25 countries

Background: Balancing opioid stewardship and the need for adequate analgesia following discharge after surgery is challenging. This study aimed to compare the outcomes for patients discharged with opioid versus opioid-free analgesia after common surgical procedures.Methods: This international, multicentre, prospective cohort study collected data from patients undergoing common acute and elective general surgical, urological, gynaecological, and orthopaedic procedures. The primary outcomes were patient-reported time in severe pain measured on a numerical analogue scale from 0 to 100% and patient-reported satisfaction with pain relief during the first week following discharge. Data were collected by in-hospital chart review and patient telephone interview 1 week after discharge.Results: The study recruited 4273 patients from 144 centres in 25 countries; 1311 patients (30.7%) were prescribed opioid analgesia at discharge. Patients reported being in severe pain for 10 (i.q.r. 1-30)% of the first week after discharge and rated satisfaction with analgesia as 90 (i.q.r. 80-100) of 100. After adjustment for confounders, opioid analgesia on discharge was independently associated with increased pain severity (risk ratio 1.52, 95% c.i. 1.31 to 1.76; P < 0.001) and re-presentation to healthcare providers owing to side-effects of medication (OR 2.38, 95% c.i. 1.36 to 4.17; P = 0.004), but not with satisfaction with analgesia (beta coefficient 0.92, 95% c.i. -1.52 to 3.36; P = 0.468) compared with opioid-free analgesia. Although opioid prescribing varied greatly between high-income and low- and middle-income countries, patient-reported outcomes did not.Conclusion: Opioid analgesia prescription on surgical discharge is associated with a higher risk of re-presentation owing to side-effects of medication and increased patient-reported pain, but not with changes in patient-reported satisfaction. Opioid-free discharge analgesia should be adopted routinely

Directions in cardiovascular medical: the renin system in the diagnosis of hypertension

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Site effects of the 2002 Molise earthquake, Italy: analysis of strong motion, ambient noise, and synthetic data from 2D modelling in San Giuliano di Puglia

On October 31st and November 1st 2002 the Basso Molise area (Southern Italy) was stuck by two moderate magnitude earthquakes (Mw = 5.7 and 5.5). The epicentral area showed a high level of damage, attributable both to the high vulnerability of existing buildings and to the site effects caused geological and geomorphological setting. The town of San Giuliano di Puglia show a very peculiar departure from the local intensity pattern: it reached an intensity two degrees higher than neighbouring towns and also within the same S. Giuliano town the damage varied notably. The site response was initially evaluated by H/V ratios performed on microtremors, while a limited number of strong motion recordings from the most severe aftershocks was also available. Both data sets indicate the simultaneous presence of two amplification effects: one around 6 Hz (that previous studied attributed to the strong, shallow impedance contrast among landfill/clay and calcarenites), and another at 2 Hz related to the first S-wave arrivals and predominant on only one receiver component. A further study performed on weak-motion recordings showed also strong vertical amplification, thus indicating underestimation by HVSR technique. This set of phenomena has been investigated with a 2-D model, whose principal novelty is the geometry of the simulated geological structure consisting of a positive flower structure generated during the late orogenic transpressive regime. The numerical model allows to reproduce the location of occurrence, the amplitude and (partly) the frequency of the observed amplifications

3D structural and stratigraphic model of the Perth Basin, Western Australia: Implications for sub-basin evolution

The history of rifting and breakup of eastern Gondwana is recorded by the development and fill of the Perth Basin in Western Australia. Knowledge of the structural architecture and stratigraphic geometry of the Perth Basin is essential to understand the evolution of the Western Australian margin and its applications to hydrocarbon and geothermal prospects, and effective aquifer management. This study integrates existing, publicly available geological, gravity, magnetic and digital elevation data to develop the first refined, regional structural and stratigraphic interpretation of the entire onshore and offshore Perth Basin, Western Australia. This new 3D model offers formation depth and thickness predictions in areas of sparse or no data. The model shows significant heterogeneity in the preserved formation thicknesses and depths at both local and regional scales. These differences may have resulted from differential subsidence and/or differential exhumation, but the formation geometries alone cannot distinguish between these two models if any erosion has occurred. Only the Lower to Middle Jurassic stratigraphy has been minimally eroded and thereby records the net subsidence. This stratigraphic interval shows that subsidence was broadly hinged from south to north, with a greater subsidence rate in the southern and central Perth Basin. Localised differences in thicknesses across adjacent sub-basins were likely controlled by differential displacement along sub-basin bounding faults during subsidence and, subsequently, during exhumation episodes. This new 3D model of the entire Perth Basin provides a framework for numerical simulations of fluid and heat flow and large-scale tectonic analysis, such as stratigraphic forward modelling of the southwestern Australian margin

Normal fault linkage and reactivation, Dampier Sub-basin, Western Australia

The Northern Carnarvon Basin of Western Australia has experienced a polyphase deformation history during the breakup of Gondwana. Extension during the Carboniferous–Permian and a subsequent Early Jurassic rift event imposed two distinct fault systems, separated by a several kilometre-thick Triassic sedimentary sequence. Inboard areas, where Triassic sequences are thinner, Jurassic faults both detach above and also penetrate into Permian sequences. Other large-scale faults demonstrate a vertical hard/soft linkage between the two fault systems. In outboard areas where the Triassic is thicker, the relationship is less clear owing to the lower resolution of Permian sequences in seismic data. Here we undertake fault displacement analysis on three faults on the southern margin of the Exmouth Plateau to investigate the growth mechanism of Jurassic-aged faults and possible structural influence of deeper Permian faults. We find evidence of low-throw faults restricted to Mesozoic strata as more complex-segmented faults that have nucleated at a depth below that resolvable on seismic data. When considered in a regional context, the nature of faults in this study suggest oblique reactivation of the NE-trending Permian fabric, under east–west-oriented extension