Arbuscular mycorrhizal fungi community and glomalin in dry forest ecosystems, Brazil

This study aimed to evaluate the abundance and diversity of arbuscular mycorrhizal fungi (AMF) and production of soil protein related to Glomalin (PSRG) (GT- Total Glomalin; GFE - easily extractable Glomalin) in Dry Forest ecosystems in North of Minas Gerais. The soil samples were collected in areas with different stages of succession (early, middle, late and pasture) in the Dry Forest State Park in the municipality of Manga, MG. Sampling was completely random, and three simple samples were collected to form a composite sample in a total of nine composite samples by study areas. The production of AMF propagules (spores and mycelial biomass external), their diversity (total wealth and Shannon and Pielou indices) and PSRG production were stimulated in early succession areas (early stage and pasture), which were equivalent or better to areas in more advanced succession stages (intermediate and late). Such pattern may contribute to the establishment of early succession plant species, which often have high mycorrhizal dependency, and consequently advance the successional process.Este trabalho teve como objetivo avaliar a abundância e diversidade de fungos micorrízicos arbusculares (FMA), bem como a produção de proteína do solo relacionada à glomalina (PSRG) (GT- glomalina total; GFE – glomalina facilmente extraível) em ecossistemas de Mata Seca no Norte de Minas Gerais. As amostras de terra foram coletadas em áreas com diferentes estádios de sucessão (inicial, intermediária, tardia e pasto) no Parque Estadual da Mata Seca no município de Manga, MG. A amostragem foi inteiramente casualizada, sendo coletadas três amostras simples para constituir uma composta, em um total de nove amostras compostas por área de estudo. Verificou-se que as áreas em inicio de sucessão (estádio inicial e pasto) estão estimulando a produção de propágulos de FMAs (esporos e biomassa de micélio externo), bem como a sua diversidade (riqueza total e índices de Shannon e Pielou) e produção de PSRG, as quais se encontram equivalentes ou superiores as áreas em estádios mais avançados (intermediário e tardio) de sucessão. Tal padrão pode contribuir para o estabelecimento de espécies vegetais de início de sucessão, que costumam apresentar elevada dependência micorrízica, e consequente avanço do processo sucessional

Composition of the soil fauna community and leaf litter stock in agro-forestry systems and secondary forestry

Agro-Forestry Systems (AFS), in addition to being a means of providing income, can be considered an alternative way of helping conserve biodiversity, both above and below ground. The aim of this study was to evaluate the accumulation of tree leaf litter and its nutrient content as well as the composition of fauna in the soil-litter layer in two Agro-Forestry Systems (AFS-1 and AFS-2), using as reference an area of secondary Forest (SF), in the Quilombola do Campinho da Independência community, Paraty, in the state of Rio de Janeiro (RJ), Brazil. The collection of accumulated leaf litter and soil-litter fauna was carried out from four sample points in each area and at two different seasons of the year, the rainy and the dry season. The leaf litter stock and its nutrient content (Ca, Mg, P, K and N) were measured. The extraction of fauna was carried out according to Tüllgren’s method, modifying the Berlese funnel. The stock of accumulated leaf litter differed between the two areas only in the rainy season; the amounts were smaller in AFS-2. In general, the Agro-Forestry Systems were very similar to the secondary forest in terms of levels and/or stock of nutrients in the majority of cases, at least in one of the seasons studied. The composition of the soil fauna groups in the Agro-Forestry System (AFS-1 and AFS-2) had a high degree of similarity to that found in the forest, and these systems mainly favour the populations of groups like Collembola and Formicidae

POPULATION DYNAMICS OF THE MICROBIOTA IN THE LITTER OF TWO TREE SPECIES OF THE ATLANTIC FOREST

This study analyzes microbiota population dynamics as a function of nutrient release rate during litter decomposition. For that, we observed two tree species native to the Atlantic Forest: brazilwood (Paubrasilia echinata) and inga (Inga laurina). To assess nitrogen (N), phosphorus (P), and potassium (K) release rates from the litter, we performed six collections over 365 days. In these collections, we placed polyvinyl bags called ‘litter bags’ below the treetops of the chosen species to collect dry leaves. To identify the groups of litter microorganisms (fungi, bacteria, and actinomycetes), we used the plate culture method to count the number of colony-forming units (CFU), and the fatty acid profile method, through biomarkers, associating nutrient release rate and abiotic factors (temperature and rainfall). Nutrient release rate correlates with litter decomposition at 140 days, and most microorganisms correlate with litter decomposition at 30 days. Nitrogen and phosphorus release rates correlate with rainfall. Fungi correlate with P release rate in inga litter decomposition. The bacteria biomarker 17:1 was the only one that correlated with N and P release rates. In conclusion, rainfall affects nutrient solubilization in the studied species, and microbiota differs between the species. When comparing the two methods to identify these microorganisms, information from one method complements information from the other, since both provide different but interdependent data. This study analyzes microbiota population dynamics as a function of nutrient release rate during litter decomposition. For that, we observed two tree species native to the Atlantic Forest: brazilwood (Paubrasilia echinata) and inga (Inga laurina). To assess nitrogen (N), phosphorus (P), and potassium (K) release rates from the litter, we performed six collections over 365 days. In these collections, we placed polyvinyl bags called ‘litter bags’ below the treetops of the chosen species to collect dry leaves. To identify the groups of litter microorganisms (fungi, bacteria, and actinomycetes), we used the plate culture method to count the number of colony-forming units (CFU), and the fatty acid profile method, through biomarkers, associating nutrient release rate and abiotic factors (temperature and rainfall). Nutrient release rate correlates with litter decomposition at 140 days, and most microorganisms correlate with litter decomposition at 30 days. Nitrogen and phosphorus release rates correlate with rainfall. Fungi correlate with P release rate in inga litter decomposition. The bacteria biomarker 17:1 was the only one that correlated with N and P release rates. In conclusion, rainfall affects nutrient solubilization in the studied species, and microbiota differs between the species. When comparing the two methods to identify these microorganisms, information from one method complements information from the other, since both provide different but interdependent data

PHYSICAL, CHEMICAL, AND BIOLOGICAL SOIL ATTRIBUTES UNDER ANALOG AGROFORESTRY SYSTEM AND PASTURE SITES

Analog agroforestry system uses native tree species to improve soil conditions and the microclimate of degraded areas. This study aimed to assess the impact of analog agroforestry on physical, chemical, and biological soil attributes. We tested the hypothesis that some of these attributes can be used as indicators of soil quality improvement compared to a managed pasture area. Two experimental sites were selected, an analog agroforestry site and a pasture site. In October 2016 (end of the dry season), soil samples were collected from the 0–5 and 5–10 cm depths and the soil fauna community was sampled using pitfall traps. The analog agroforestry system led to increased total abundance, total richness, mean richness, evenness, and diversity of the soil fauna community as well as higher gravimetric soil moisture, sand content, pH, calcium, magnesium, and sum of exchangeable bases, which are good indicators of soil quality. Adults of Coleoptera, Diptera, Gastropoda, Hymenoptera, Isopoda, Lepidoptera, Poduromorpha, Symphypleona, Pseudoscorpionida, Lepidoptera and larvae of Coleoptera, Diptera, Lepidoptera, and Neuroptera were the most abundant taxonomic groups in the analog agroforestry system

FERTILIDADE DO SOLO E SUBSTÂNCIAS HÚMICAS EM ÁREA DE CAVA DE EXTRAÇÃO DE ARGILA REVEGETADA COM EUCALIPTO E LEGUMINOSAS NO NORTE FLUMINENSE

The revegetation of areas of clay extraction with eucalyptus and legumes can promote the improvement of soil chemical atributtes. The aim of this study was to evaluate the influence of pure plantations and intercropping of legumes and eucalyptus on soil fertility and the content of total organic carbon and humic substances, in a mining digging clay in North Fluminense. The original soil dig area under study is a Inceptsol where the surface layer (thickness of 20 cm approximately), with higher concentrations of organic matter was removed and returned to the bottom of the dig after the extraction of clay. The dig was leveled mechanically and kept under fallow for two years. Revegetation was performed with the species Eucalyptus camaldulensis (Euc), Acacia mangium (Ac) and Sesbania virgata (Sesb), in pure and intercropped. The experimental design was a randomized block design with seven treatments (pure stands of Euc, Ac and Sesb; Intercropping of Euc + Ac + Euc Sesb, Ac + Sesb, and degraded area with natural vegetation (DANV)) and three replications. At 48 months after implantation of the systems were sampled soil layer from 0.00 to 0.05 m, which evaluated some variables of soil fertility (pH, P, K+, Ca+2, Mg+2, Na+, Al+3, H + Al), total organic carbon (TOC), nitrogen (N) and quantified the carbon of humic substances in the humin carbon (C-HUM), humic acid carbon (C-FAH ) and fulvic acid (C-FAF). It was observed that all stands promoted increases in the concentration Ca, Mg, P, K, N, TOC, HUM C-C-C-FAF and HAF relative to DANV. The revegetation of areas of mining digging clay in North Fluminense pure stands or mixed with eucalyptus and legumes, leads to improvements in soil fertility and carbon and total soil nitrogen and carbon of humic substances. At four years after revegetation systems are indicated, in general, to result in increases in TOC, total N, Mg, sum of bases and cation exchange capacity, there is the planting of acacia plantations among pure, and the planting of eucalyptus + sesbania, among Intercropping. In addition, the consortium eucalyptus + sesbania, presented one of the highest levels of C humin fraction.http://dx.doi.org/10.5902/1980509819606A revegetação de áreas de extração de argila com eucalipto e leguminosas pode favorecer a melhoria dos atributos químicos do solo. O objetivo do trabalho foi avaliar a influência de plantios puros e consorciados de leguminosas e eucalipto na fertilidade do solo e nos teores de carbono orgânico total e das substâncias húmicas, em uma cava de extração de argila no Norte Fluminense. O solo original da área da cava em estudo é um Cambissolo Háplico Sódico gleico, no qual a camada superficial (espessura de 20 cm, aproximadamente), com maiores teores de matéria orgânica, foi retirada e devolvida ao fundo da cava após a extração da argila. A cava foi nivelada mecanicamente e mantida sob pousio durante dois anos. A revegetação foi realizada com as espécies Eucalyptus camaldulensis (Euc), Acacia mangium (Ac) e Sesbania virgata (Sesb), em plantios puros e consorciados. O delineamento experimental utilizado foi o de blocos casualizados com sete tratamentos (plantios puros de Euc, Ac e Sesb; plantios consorciados de Euc + Ac, Euc + Sesb, Ac + Sesb; e área degradada com vegetação espontânea (ADVE)) e três repetições. Aos 48 meses após implantação dos sistemas foram coletadas amostras de solo na camada de 0,00 a 0,05 m, em que se avaliaram algumas variáveis da fertilidade do solo (pH em água, P, K+; Ca+2, Mg+2, Na+, Al+3, H+Al), carbono orgânico total (COT), nitrogênio (N) e quantificado o carbono das substâncias húmicas em carbono da humina (C-HUM), carbono da fração ácido húmico (C-FAH) e ácido fúlvico (C-FAF). Observou-se que todos os plantios promoveram aumentos nos teores Ca, Mg, P, K, N, COT, C-HUM, C-FAH e C-FAF em relação à ADVE. A revegetação de áreas de cava de extração de argila no Norte Fluminense com plantios puros ou consorciados de eucalipto e leguminosas, acarreta em melhorias da fertilidade do solo e dos teores de carbono e nitrogênio totais do solo e do carbono das substâncias húmicas. Aos quatro anos após a revegetação, os sistemas mais indicados, de uma forma geral, para acarretar em aumentos dos teores de COT, N total, Mg, soma de bases e capacidade de troca catiônica, destaca-se o plantio de acácia, dentre os plantios puros, e o plantio de eucalipto + sesbânia, dentre os plantios consorciados. Além disso, o consórcio eucalipto + sesbânia, apresentou um dos maiores teores C da fração humina

FUNGOS MICORRÍZICOS ARBUSCULARES: COMPOSIÇÃO, COMPRIMENTO DE MICÉLIO EXTRARRADICULAR E GLOMALINA EM ÁREAS DE MATA ATLÂNTICA, RIO DE JANEIRO

The Atlantic Forest is considered an environment of great biodiversity. However, by constantly being subjected to intense human pressure, is very fragmented and disconnected, surrounded by extensive matrices formed by pastures, capoeiras, monocultures and urban areas. Fragmentation, as well as certain uses and soil management can cause changes in the composition and activity of soil microorganisms, such as mycorrhizal fungi (AMF). Fragmentation, as well as certain uses and soil management may entail a reduction of edaphic microflora. Thus, the study aimed to evaluate the composition of the population of mycorrhizal fungi (MF) in different areas, to determine the influence of vegetation on the abundance of AMF spores in the length of extraradical mycelium (LEM) and levels of Glomalin-related soil protein (GRSP) the ground. The systems were evaluated: annual crops (Anag); perennial agriculture (PAG), pasture, secondary forest early stage (SFES), secondary forest medium stage (SFME); advanced stage secondary forest (ASSF). Soil samples were collected at a depth of 0-5 cm, in two different seasons (summer and winter), and analyzed the abundance of spores and species composition of AMF, the LEM, and the presence of GRSP. It was recovered a total of 27 morphotypes of spores of AMF, and AMF species was that which was present in all areas studied in both seasons and in most cases with a high frequency of occurrence. The abundance of AMF spores was influenced by the type of vegetation. A low AMF sporulation was observed in the area of Anag both stations. The agricultural areas have changed the species composition of AMF in relation to forest systems. The concentration of GRSP - easily extractable (GRSP-EE) fell in the SFME for agriculture.A Mata Atlântica é considerada um ambiente de ampla biodiversidade, no entanto, por estar constantemente sendo submetida a uma intensa pressão antrópica, encontra-se bastante fragmentada e desconectada, circundada por extensas matrizes formadas de pastos, capoeiras, monoculturas e áreas urbanas. A fragmentação, bem como determinados sistemas de uso e manejo do solo, pode ocasionar alterações na composição e atividade de microrganismos do solo, como os fungos micorrízicos arbusculares (FMAs). Dessa forma, o trabalho teve como objetivo avaliar a composição da população dos FMAs em diferentes áreas de Mata Atlântica, determinar a influência do tipo de cobertura vegetal na abundância de esporos de FMAs, no comprimento de micélio extrarradicular (CMET) e nos teores de proteína do solo relacionada à glomalina no solo (PSRG). Os sistemas avaliados foram: agricultura anual (AgAn); agricultura perene (AgP), pasto, floresta secundária estádio inicial (FSEI); floresta secundária estádio médio (FSEM); floresta secundária estádio avançado (FSEA). Coletaram-se amostras de terra na profundidade de 0–5 cm, em duas épocas distintas (verão e inverno). Um total de 27 morfotipos de esporos de FMAs foi coletado, sendo que a espécie Glomus macrocarpum Tulasne & Tulasne foi presente em todas as áreas estudadas, em ambas as épocas de coleta e na maioria das vezes com uma elevada frequência de ocorrência. A abundância de esporos de FMAs foi influenciada pelo tipo de cobertura vegetal. Uma baixa esporulação dos FMAs foi observada na área de AgAn em ambas as coletas. As áreas agrícolas modificaram a composição das espécies de FMAs em relação aos sistemas florestais. Os teores de PSRG - facilmente extraível (PSRG-FE) reduziram-se quando da passagem de FSEM para agricultura.

PRODUCTION OF Eucalyptus urophylla x Eucalyptus grandis SEEDLINGS WITH DIFFERENT FERTILIZERS

Mineral fertilization is an important practice that contributes to the production of seedlings with satisfactory quality. This study aimed to evaluate the effect of the application of potassium chloride (KCl) and monoammonium phosphate (MAP) on growth and nutrition of Eucalyptus urophylla x Eucalyptus grandis seedlings. The experiment was conducted in full sun, with direct sowing in tubes containing composite substrate (Pinus bark, coconut fiber, bovine manure and vermiculite). The experimental design was a randomized block design in a 5 x 2 factorial scheme, with five doses of KCl (0.0, 0.025, 0.050, 0.100, and 0.200 g seedling-1) and two doses of MAP (0 and 0.10g seedling-1), with four replicates. At 120 days, the effect of the interaction between the KCl and MAP doses was verified for the variables: diameter at root collar, dry mass of the aerial part (DMAP) and total (TDM), Dickson Quality Index, N content, P K in the aerial part, K content in the roots, absorption efficiency (AE) of N and P. For the variables: height (H); leaf area; root dry mass (RDM); H/DRC and DMPA/RDM ratios; and N and P content in the roots, only effect of the application of MAP is observed, whereas for AE_K the effect was only of KCl. Thus, there is variation in the effect of KCl doses as a function of morphological and nutritional variables evaluated. In general, the best seedling growth and nutrition occurs with the applied dose of 0.100 g KCl + 0.10 g MAP seedling -1

ARBUSCULAR MYCORRHIZAL FUNGI: COMPOSITION, LENGHT EXTRARADICAL MYCELIUM AND GLOMALIN IN AREAS OF ATLANTIC FOREST, RIO DE JANEIRO

A Mata Atl\ue2ntica \ue9 considerada um ambiente de ampla biodiversidade, no entanto, por estar constantemente sendo submetida a uma intensa press\ue3o antr\uf3pica, encontra-se bastante fragmentada e desconectada, circundada por extensas matrizes formadas de pastos, capoeiras, monoculturas e \ue1reas urbanas. A fragmenta\ue7\ue3o, bem como determinados sistemas de uso e manejo do solo, pode ocasionar altera\ue7\uf5es na composi\ue7\ue3o e atividade de microrganismos do solo, como os fungos micorr\uedzicos arbusculares (FMAs). Dessa forma, o trabalho teve como objetivo avaliar a composi\ue7\ue3o da popula\ue7\ue3o dos FMAs em diferentes \ue1reas de Mata Atl\ue2ntica, determinar a influ\ueancia do tipo de cobertura vegetal na abund\ue2ncia de esporos de FMAs, no comprimento de mic\ue9lio extrarradicular (CMET) e nos teores de prote\uedna do solo relacionada \ue0 glomalina no solo (PSRG). Os sistemas avaliados foram: agricultura anual (AgAn); agricultura perene (AgP), pasto, floresta secund\ue1ria est\ue1dio inicial (FSEI); floresta secund\ue1ria est\ue1dio m\ue9dio (FSEM); floresta secund\ue1ria est\ue1dio avan\ue7ado (FSEA). Coletaram-se amostras de terra na profundidade de 0\u20135 cm, em duas \ue9pocas distintas (ver\ue3o e inverno). Um total de 27 morfotipos de esporos de FMAs foi coletado, sendo que a esp\ue9cie Glomus macrocarpum Tulasne & Tulasne foi presente em todas as \ue1reas estudadas, em ambas as \ue9pocas de coleta e na maioria das vezes com uma elevada frequ\ueancia de ocorr\ueancia. A abund\ue2ncia de esporos de FMAs foi influenciada pelo tipo de cobertura vegetal. Uma baixa esporula\ue7\ue3o dos FMAs foi observada na \ue1rea de AgAn em ambas as coletas. As \ue1reas agr\uedcolas modificaram a composi\ue7\ue3o das esp\ue9cies de FMAs em rela\ue7\ue3o aos sistemas florestais. Os teores de PSRG - facilmente extra\uedvel (PSRG-FE) reduziram-se quando da passagem de FSEM para agricultura.The Atlantic Forest is considered an environment of great biodiversity. However, by constantly being subjected to intense human pressure, is very fragmented and disconnected, surrounded by extensive matrices formed by pastures, capoeiras, monocultures and urban areas. Fragmentation, as well as certain uses and soil management can cause changes in the composition and activity of soil microorganisms, such as mycorrhizal fungi (AMF). Fragmentation, as well as certain uses and soil management may entail a reduction of edaphic microflora. Thus, the study aimed to evaluate the composition of the population of mycorrhizal fungi (MF) in different areas, to determine the influence of vegetation on the abundance of AMF spores in the length of extraradical mycelium (LEM) and levels of Glomalin-related soil protein (GRSP) the ground. The systems were evaluated: annual crops (Anag); perennial agriculture (PAG), pasture, secondary forest early stage (SFES), secondary forest medium stage (SFME); advanced stage secondary forest (ASSF). Soil samples were collected at a depth of 0-5 cm, in two different seasons (summer and winter), and analyzed the abundance of spores and species composition of AMF, the LEM, and the presence of GRSP. It was recovered a total of 27 morphotypes of spores of AMF, and AMF species was that which was present in all areas studied in both seasons and in most cases with a high frequency of occurrence. The abundance of AMF spores was influenced by the type of vegetation. A low AMF sporulation was observed in the area of Anag both stations. The agricultural areas have changed the species composition of AMF in relation to forest systems. The concentration of GRSP - easily extractable (GRSP-EE) fell in the SFME for agriculture

Soil organic matter fractions, chemical attributes and aggregation under forestry and agricultural systems

The aim of this work was to evaluate the influence of the conversion of forest systems to agricultural systems in the organic matter compartments, aggregation and soil chemical attributes, in the Atlantic Forest. The evaluated systems were: annual crop (ACr); perennial agriculture (PAg); pasture; and secondary forest early (SFES), medium (SFMS), and advanced stage (SFAS). Soil samples were collected at the layer of 0-5 cm depth and quantified the total organic carbon (TOC), C of humic substances, oxidizable C, granulometric fractions of soil organic matter (SOM), soil chemical attributes, soil aggregation and glomalin-related soil protein (GRSP-total and GRSP-easily extractable) in different aggregate classes. It was observed a reduction of the TOC, particulate organic carbon (POC), humic substances and oxidizable C in the PAg and ACr areas comparing to pasture and forest systems. Moreover, the pH values increased whereas P content decreased in comparison with SFAS. As for aggregation, the PAg and the ACr decreased by around 35% and 20% the mean weight diameter of aggregates, respectively, compared to the average values found in the forestry systems, and 34% and 45%, respectively in relation to pasture. In general, GRSP-total were reduced by agriculture. Thus, it appears that the agriculture which has been practiced is altering negatively the soil chemical, physical and biological attributes.The aim of this work was to evaluate the influence of the conversion of forest systems to agricultural systems in the organic matter compartments, aggregation and soil chemical attributes, in the Atlantic Forest. The evaluated systems were: annual crop (ACr); perennial agriculture (PAg); pasture; and secondary forest early (SFES), medium (SFMS), and advanced stage (SFAS). Soil samples were collected at the layer of 0-5 cm depth and quantified the total organic carbon (TOC), C of humic substances, oxidizable C, granulometric fractions of soil organic matter (SOM), soil chemical attributes, soil aggregation and glomalin-related soil protein (GRSP-total and GRSP-easily extractable) in different aggregate classes. It was observed a reduction of the TOC, particulate organic carbon (POC), humic substances and oxidizable C in the PAg and ACr areas comparing to pasture and forest systems. Moreover, the pH values increased whereas P content decreased in comparison with SFAS. As for aggregation, the PAg and the ACr decreased by around 35% and 20% the mean weight diameter of aggregates, respectively, compared to the average values found in the forestry systems, and 34% and 45%, respectively in relation to pasture. In general, GRSP-total were reduced by agriculture. Thus, it appears that the agriculture which has been practiced is altering negatively the soil chemical, physical and biological attributes

SOIL FERTILITY AND HUMIC SUBSTANCES IN AN AREA OF CLAY EXTRACTION REVEGETATED WITH EUCALYPT AND LEGUMES IN THE NORTH OF RIO DE JANEIRO STATE

A revegeta\ue7\ue3o de \ue1reas de extra\ue7\ue3o de argila com eucalipto e leguminosas pode favorecer a melhoria dos atributos qu\uedmicos do solo. O objetivo do trabalho foi avaliar a influ\ueancia de plantios puros e consorciados de leguminosas e eucalipto na fertilidade do solo e nos teores de carbono org\ue2nico total e das subst\ue2ncias h\ufamicas, em uma cava de extra\ue7\ue3o de argila no Norte Fluminense. O solo original da \ue1rea da cava em estudo \ue9 um Cambissolo H\ue1plico S\uf3dico gleico, no qual a camada superficial (espessura de 20 cm, aproximadamente), com maiores teores de mat\ue9ria org\ue2nica, foi retirada e devolvida ao fundo da cava ap\uf3s a extra\ue7\ue3o da argila. A cava foi nivelada mecanicamente e mantida sob pousio durante dois anos. A revegeta\ue7\ue3o foi realizada com as esp\ue9cies Genus camaldulensis (Euc), Acacia mangium (Ac) e Sesbania virgata (Sesb), em plantios puros e consorciados. O delineamento experimental utilizado foi o de blocos casualizados com sete tratamentos (plantios puros de Euc, Ac e Sesb; plantios consorciados de Euc + Ac, Euc + Sesb, Ac + Sesb; e \ue1rea degradada com vegeta\ue7\ue3o espont\ue2nea (ADVE)) e tr\ueas repeti\ue7\uf5es. Aos 48 meses ap\uf3s implanta\ue7\ue3o dos sistemas foram coletadas amostras de solo na camada de 0,00 a 0,05 m, em que se avaliaram algumas vari\ue1veis da fertilidade do solo (pH em \ue1gua, P, K+; Ca+2, Mg+2, Na+, Al+3, H+Al), carbono org\ue2nico total (COT), nitrog\ueanio (N) e quantificado o carbono das subst\ue2ncias h\ufamicas em carbono da humina (C-HUM), carbono da fra\ue7\ue3o \ue1cido h\ufamico (C-FAH) e \ue1cido f\ufalvico (C-FAF). Observou-se que todos os plantios promoveram aumentos nos teores Ca, Mg, P, K, N, COT, C-HUM, C-FAH e C-FAF em rela\ue7\ue3o \ue0 ADVE. A revegeta\ue7\ue3o de \ue1reas de cava de extra\ue7\ue3o de argila no Norte Fluminense com plantios puros ou consorciados de eucalipto e leguminosas, acarreta em melhorias da fertilidade do solo e dos teores de carbono e nitrog\ueanio totais do solo e do carbono das subst\ue2ncias h\ufamicas. Aos quatro anos ap\uf3s a revegeta\ue7\ue3o, os sistemas mais indicados, de uma forma geral, para acarretar em aumentos dos teores de COT, N total, Mg, soma de bases e capacidade de troca cati\uf4nica, destaca-se o plantio de ac\ue1cia, dentre os plantios puros, e o plantio de eucalipto + sesb\ue2nia, dentre os plantios consorciados.Al\ue9m disso, o cons\uf3rcio eucalipto + sesb\ue2nia, apresentou um dos maiores teores C da fra\ue7\ue3o humina.The revegetation of areas of clay extraction with eucalypt and legumes can promote the improvement of soil chemical atributtes. The aim of this study was to evaluate the influence of pure plantations and intercropping of legumes and eucalypt on soil fertility and the content of total organic carbon and humic substances, in a mining digging clay in North Fluminense. The original soil dig area under study is a Inceptsol where the surface layer (thickness of 20 cm approximately), with higher concentrations of organic matter was removed and returned to the bottom of the dig after the extraction of clay. The dig was leveled mechanically and kept under fallow for two years. Revegetation was performed with the species Eucalyptus camaldulensis (Euc), Acacia mangium (Ac) and Sesbania virgata (Sesb), in pure and intercropped. The experimental design was a randomized block design with seven treatments (pure stands of Euc, Ac and Sesb; Intercropping of Euc + Ac + Euc Sesb, Ac + Sesb, and degraded area with natural vegetation (DANV)) and three replications. At 48 months after implantation of the systems were sampled soil layer from 0.00 to 0.05 m, which evaluated some variables of soil fertility (pH, P, K+, Ca+2, Mg+2, Na+, Al+3, H + Al), total organic carbon (TOC), nitrogen (N) and quantified the carbon of humic substances in the humin carbon (C-HUM), humic acid carbon (C-FAH ) and fulvic acid (C-FAF). It was observed that all stands promoted increases in the concentration Ca, Mg, P, K, N, TOC, HUM C-C-C-FAF and HAF relative to DANV. The revegetation of areas of mining digging clay in North Fluminense pure stands or mixed with eucalypt and legumes, leads to improvements in soil fertility and carbon and total soil nitrogen and carbon of humic substances. At four years after revegetation systems are indicated, in general, to result in increases in TOC, total N, Mg, sum of bases and cation exchange capacity, there is the planting of acacia plantations among pure, and the planting of eucalypt + sesbania, among Intercropping. In addition, the consortium eucalypt + sesbania, presented one of the highest levels of C humin fraction