Low Gravity Flight Complement Data

The structural and mechanical design and performance requirements for a space transportation system carrier which will accommodate essentially self-supporting low-g MEA and MAUS facilities are described. Also included are the mission requirements for the materials processing facility and MEA/MAUS experiment flight implementation reguirements

Stress-concentration factors for finite orthotropic laminates with a circular hole and uniaxial loading

Stresses were calculated for finite-width orthotropic laminates with a circular hole and remote uniaxial loading using a two-dimensional finite element analysis with both uniform stress and uniform displacement boundary conditions. Five different laminates were analyzed. Computed results are presented for selected combinations of hole diameter/sheet-width ratio d/w and length-to-width ratio L/w. For small L/w values, the stress-concentration factors K sub tn were significantly different for the uniform stress and uniform displacement boundary conditions. Typically, for the uniform stress condition, the K sub tn values were much larger than for the infinite strip reference condition; however, for the uniform displacement condition, they were only slightly smaller than for this reference. The results for long strips are also presented as width correction factors. For d/w less or = 0.33, these width correction factors are nearly equal for all five laminates

An evaluation of potential locations for AROD ground stations

Airborne Range and Orbit Determination tracking stations potential locations evaluation and accuracy during early minutes of fligh

Stress-concentration factors for finite orthotropic laminates with a pin-loaded hole

Stresses were calculated for finite size orthotropic laminates loaded by a frictionless steel pin in a circular hole of the same diameter. The calculations were based on finite element analyses for six laminates. Stress concentration factors, based on nominal bearing stress, were determined for wide ranges of the ratios of width to diameter, w/d and edge distance to diameter, e/d. An infinite laminate case was analyzed for each laminate. Orthotropy had a significant influence on the tensile stress concentration at the hole. For example, the stress concentration factors for the infinite laminate cases ranged from 0.82 to 2.16, compared with 0.87 for the quasi-isotropic laminate. The finite widths and edge distances strongly influenced the tensile stress concentration. For the practical range w/d or = 3, the peak tensile stresses were as much as 80% larger than the infinite laminate reference value. For e/d or = 3, these stresses were amplified by as much as 50%. In contrast, the finite width and edge distance had little effect on shear-out and bearing stress concentrations

Determination of trajectory parameters relative to various AROD ground stations

Trajectory parameter determination in relation to various AROD airborne range and orbital determination ground station

Variations of a global constraint factor in cracked bodies under tension and bending loads

Elastic-plastic finite-element analyses were used to calculate stresses and displacements around a crack in finite-thickness plates for an elastic-perfectly plastic material. Middle- and edge-crack specimens were analyzed under tension and bending loads. Specimens were 1.25 to 20 mm thick with various widths and crack lengths. A global constraint factor alpha(sub g), an averaged normal-stress to flow-stress ratio over the plastic region, was defined to simulate three-dimensional (3D) effects in two-dimensional (2D) models. For crack lengths and uncracked ligament lengths greater than four times the thickness, the global constraint factor was found to be nearly a unique function of a normalized stress-intensity factor (related to plastic-zone size to thickness ratio) from small- to large-scale yielding conditions for various specimen types and thickness. For crack length-to-thickness ratios less than four, the global constraint factor was specimen type, crack length and thickness dependent. Using a 2D strip-yield model and the global constraint factors, plastic-zone sizes and crack-tip displacements agreed reasonably well with the 3D analyses. For a thin sheet aluminum alloy, the critical crack-tip-opening angle during stable tearing was found to be independent of specimen type and crack length for crack length-to-thickness ratios greater than 4

REVIEW: Life-cycle, total-industry genetic improvement of feed efficiency in beef cattle: Blueprint for the Beef Improvement Federation

On a life-cycle basis, beef animals are able to consume large amounts of low-cost, low-quality forages relative to higher-cost concentrates compared with pigs and chickens. However, of the 3, beef is still more expensive to produce on a cost–per–edible pound basis. Accordingly, there is need for genetic programs and management changes that will improve efficiency, sustainability, and profitability of beef production. Options include improving reproductive rate, reducing feed used for maintenance, or both, while not reducing output. A goal for improving efficiency of feed utilization is to reduce the amount or proportion of feed used for maintenance. Such reduction is a target for genetic improvement, but such a goal does not include defining a single measure of efficiency. A single efficiency measure would likely lead to single-trait selection and not account for any potentially antagonistic effects on other production characteristics. Because we are not able to explain all variation in individual-animal intake from only knowledge of BW maintained and level of production, measuring feed intake is necessary. Therefore, our recommendation is that national cattle evaluation systems analyze feed intake as an economically relevant trait with incorporation of appropriate indicator traits for an EPD for feed intake requirements that could then be used in a multiple-trait setting such as in a selection index. With improvements in technology for measurement of feed intake, individual measures of feed intake should continually be collected to facilitate development of genetic predictors that enhance accuracy of prediction of progeny differences in national cattle evaluations

Mastermind Mutations Generate a Unique Constellation of Midline Cells within the Drosophila CNS

Background: The Notch pathway functions repeatedly during the development of the central nervous system in metazoan organisms to control cell fate and regulate cell proliferation and asymmetric cell divisions. Within the Drosophila midline cell lineage, which bisects the two symmetrical halves of the central nervous system, Notch is required for initial cell specification and subsequent differentiation of many midline lineages. Methodology/Principal Findings: Here, we provide the first description of the role of the Notch co-factor, mastermind, in the central nervous system midline of Drosophila. Overall, zygotic mastermind mutations cause an increase in midline cell number and decrease in midline cell diversity. Compared to mutations in other components of the Notch signaling pathway, such as Notch itself and Delta, zygotic mutations in mastermind cause the production of a unique constellation of midline cell types. The major difference is that midline glia form normally in zygotic mastermind mutants, but not in Notch and Delta mutants. Moreover, during late embryogenesis, extra anterior midline glia survive in zygotic mastermind mutants compared to wild type embryos. Conclusions/Significance: This is an example of a mutation in a signaling pathway cofactor producing a distinct centra

Biological variability dominates and influences analytical variance in HPLC-ECD studies of the human plasma metabolome

<p>Abstract</p> <p>Background</p> <p>Biomarker-based assessments of biological samples are widespread in clinical, pre-clinical, and epidemiological investigations. We previously developed serum metabolomic profiles assessed by HPLC-separations coupled with coulometric array detection that can accurately identify <it>ad libitum </it>fed and caloric-restricted rats. These profiles are being adapted for human epidemiology studies, given the importance of energy balance in human disease.</p> <p>Methods</p> <p>Human plasma samples were biochemically analyzed using HPLC separations coupled with coulometric electrode array detection.</p> <p>Results</p> <p>We identified these markers/metabolites in human plasma, and then used them to determine which human samples represent blinded duplicates with 100% accuracy (N = 30 of 30). At least 47 of 61 metabolites tested were sufficiently stable for use even after 48 hours of exposure to shipping conditions. Stability of some metabolites differed between individuals (N = 10 at 0, 24, and 48 hours), suggesting the influence of some biological factors on parameters normally considered as analytical.</p> <p>Conclusion</p> <p>Overall analytical precision (mean median CV, ~9%) and total between-person variation (median CV, ~50–70%) appear well suited to enable use of metabolomics markers in human clinical trials and epidemiological studies, including studies of the effect of caloric intake and balance on long-term cancer risk.</p

Correlations of Gene Expression with Blood Lead Levels in Children with Autism Compared to Typically Developing Controls

The objective of this study was to examine the correlation between gene expression and lead (Pb) levels in blood in children with autism (AU, n = 37) compared to typically developing controls (TD, n = 15). We postulated that, though lead levels did not differ between the groups, AU children might metabolize lead differently compared to TD children. RNA was isolated from blood and processed on Affymetrix microarrays. Separate analyses of covariance (ANCOVA) corrected for age and gender were performed for TD, AU, and all subjects (AU + TD). To reduce false positives, only genes that overlapped these three ANCOVAs were considered. Thus, 48 probe sets correlated with lead levels in both AU and TD subjects and were significantly different between the groups (p(Diagnosis × log2 Pb) < 0.05). These genes were related mainly to immune and inflammatory processes, including MHC Class II family members and CD74. A large number (n = 791) of probe sets correlated (P ≤ 0.05) with lead levels in TD but not in AU subjects; and many probe sets (n = 162) correlated (P ≤ 0.05) with lead levels in AU but not in TD subjects. Only 30 probe sets correlated (P ≤ 0.05) with lead levels in a similar manner in the AU and TD groups. These data show that AU and TD children display different associations between transcript levels and low levels of lead. We postulate that this may relate to the underlying genetic differences between the two groups, though other explanations cannot be excluded