DETRÁS DE LA APARIENCIA

Behind the appearance gives a title to a series of works that arise from the need to foster the intuitive, imaginative and emotional capacity of our innermost being through a deep plastic analysis of the natural landscape It is through a methodological process that is adjusted to our particular experience of the natural environment as it is intended to carry out the production of a series of unpublished works that are based on a theoretical basis. The three main pillars on which this research will be based are: the use of intuition as a methodological tool, plastic experimentation and continuous formal renovation of the painting itself and the attempt to go beyond the evidence, predominating an informalist tendency as a strategy Creative and conceptual. We approach this experimentation from the pictorial matter, elaborated mainly with acrylic and agglutinated lands with latex.Detrás de la apariencia da título a una serie de trabajos que nacen de la necesidad por fomentar la capacidad intuitiva, imaginativa y emocional de nuestro ser más íntimo, a través de un profundo análisis plástico del paisaje natural. Es mediante un proceso metodológico que se ajuste a nuestra particular vivencia del entorno natural como se pretende llevar a cabo la producción de una serie de obras inéditas que estén sustentadas en una base teórica. Los tres principales pilares en los que se fundamentará esta investigación son: el uso de la intuición como herramienta metodológica, la experimentación plástica y renovación formal continua de la propia pintura y el intento por ir más allá de la evidencia, predominando una tendencia informalista como estrategia creativa y conceptual. Abordamos esta experimentación a partir de la materia pictórica, elaborada mayoritariamente con acrílico y tierras aglutinadas con látex.Pérez Cremades, J. (2017). DETRÁS DE LA APARIENCIA. http://hdl.handle.net/10251/92002TFG

Assessment of the displacement of mangrove species Avicennia sp. on mud clam Polymesoda erosa (Solander, 1876) in planted Rhizosphora mangroves

The mangrove ecosystem in the Philippines consists of 35-40 mangrove species. Mangroves use to grow on soft muddy substrate, commonly in estuaries where they can find sheltered and shallow coasts, and they can also grow in more salty waters on island shores and tidal flats The substrate is one of the most important aspects that determine mangrove distribution, and the macrofauna is one of the things which affect the substrate composition. In this study we will focus on the study of macro-invertebrate fauna (mollusc), and its ecological relationship with the mangrove species. We are going to study if the lack of plant biodiversity affect to the faunal biodiversity and the ecological quality of mangroves, and our hypothesis are that the monospecific reforestation of mangroves entails the loss of vegetal biodiversity, which is the ecological support of many other fauna and flora species. To find scientific data and references that endorse those statements, our main aim will be to statistically relate the distribution and abundance of plant and faunal biodiversity. In order to make that tangled task reachable we will focus on a few species. Those are the mangrove species Avicennia sp. and Rhyzophora sp. and the mud clam Polymesoda erosa (Solander, 1876) among other molluscs.Pérez Cremades, J. (2014). Assessment of the displacement of mangrove species Avicennia sp. on mud clam Polymesoda erosa (Solander, 1876) in planted Rhizosphora mangroves. Universitat Politècnica de València. http://hdl.handle.net/10251/47217Archivo delegad

Mechanisms underlying the influence of oestrogen on cardiovascular physiology in women

Women show a lower incidence of cardiovascular diseases than aged-matched men, but this benefit disappears after menopause. Oestrogen-mediated vascular actions are mainly attributed to oestradiol and exerted by oestrogen receptors (ERα, ERβ, and GPER), through rapid and/or genomic mechanisms, but these effects depend on ageing and inflammation. A cardiovascular approach in women's health has arisen due to controversy regarding oestrogens' beneficial impact as reported in experimental and observational studies and large randomized trials. These can be explained, in part, by two mutually non-exclusive hypotheses. On the one hand, the timing hypothesis, which states that oestrogen-mediated benefits occur before the detrimental effects of ageing are established in the vasculature; on the other hand, ageing and/or hormonal-associated changes in ER expression that could lead to a deleterious imbalance in favour of ERβ over ERα, generally associated with higher inflammation and endothelial dysfunction. In experimental studies, oestradiol acting on ERα promotes the release of vasoactive compounds such as nitric oxide (NO) and prostacyclin, and shifts the angiotensin axis towards angiotensin 1-7 production. Mechanisms underlying oestradiol vascular function also include anti-inflammatory and epigenetic modifications. 17β-oestradiol changes the transcriptomic profile of endothelial cells, and the involvement of miRNA in the regulatory pathways of vascular function reinforces assumptions regarding the vascular actions of oestrogen. Thus, the present symposium review aims to postulate the role of ERα in oestrogen modulation of endothelial-derived mediators and vascular physiology, as well as its relationship with miRNA and inflammation, and elucidate how physiological changes in postmenopausal women counteract the observed effects

Role of miRNA in the Regulatory Mechanisms of Estrogens in Cardiovascular Ageing

Cardiovascular diseases are a worldwide health problem and are the leading cause of mortality in developed countries. Together with experimental data, the lower incidence of cardiovascular diseases in women than in men of reproductive age points to the influence of sex hormones at the cardiovascular level and suggests that estrogens play a protective role against cardiovascular disease and that this role is also modified by ageing. Estrogens affect cardiovascular function via their specific estrogen receptors to trigger gene expression changes at the transcriptional level. In addition, emerging studies have proposed a role for microRNAs in the vascular effects mediated by estrogens. miRNAs regulate gene expression by repressing translational processes and have been estimated to be involved in the regulation of approximately 30% of all protein-coding genes in mammals. In this review, we highlight the current knowledge of the role of estrogen-sensitive miRNAs, and their influence in regulating vascular ageing

miRNA as New Regulatory Mechanism of Estrogen Vascular Action

The beneficial effects of estrogen on the cardiovascular system have been reported extensively. In fact, the incidence of cardiovascular diseases in women is lower than in age-matched men during their fertile stage of life, a benefit that disappears after menopause. These sex-related differences point to sexual hormones, mainly estrogen, as possible cardiovascular protective factors. The regulation of vascular function by estrogen is mainly related to the maintenance of normal endothelial function and is mediated by both direct and indirect gene transcription through the activity of specific estrogen receptors. Some of these mechanisms are known, but many remain to be elucidated. In recent years, microRNAs have been established as non-coding RNAs that regulate the expression of a high percentage of protein-coding genes in mammals and are related to the correct function of human physiology. Moreover, within the cardiovascular system, miRNAs have been related to physiological and pathological conditions. In this review, we address what is known about the role of estrogen-regulated miRNAs and their emerging involvement in vascular biology

Q-switched all-fiber laser based on magnetostriction modulation of a Bragg grating

We report an actively Q-switched all-fiber laser based on magnetostriction modulation of a Bragg grating. The laser employs a pair of Bragg gratings as reflective mirrors, one of which is bonded to a magnetostrictive element. Lengthening of the magnetostrictive element when a magnetic field is applied shifts the Bragg wavelength of the grating, allowing control of the Q-factor of the cavity and, thus, performing active Q-switching. The magnetostrictive modulator is small, compact and requires less than 300 mW electrical drive power. Using erbium-doped fiber and a maximum pump power of 120 mW, Q-switch pulses of more than 1 W peak power were obtained, with a pulse repetition rate that can be continuously varied from 1 Hz to 125 kHz

MicroRNA as crucial regulators of gene expression in estradiol-treated human endothelial cells.

Background/Aims: Estrogen signalling plays an important role in vascular biology as it modulates vasoactive and metabolic pathways in endothelial cells. Growing evidence has also established microRNA (miRNA) as key regulators of endothelial function. Nonetheless, the role of estrogen regulation on miRNA profile in endothelial cells is poorly understood. In this study, we aimed to determine how estrogen modulates miRNA profile in human endothelial cells and to explore the role of the different estrogen receptors (ERα, ERβ and GPER) in the regulation of miRNA expression by estrogen. Methods: We used miRNA microarrays to determine global miRNA expression in human umbilical vein endothelial cells (HUVEC) exposed to a physiological concentration of estradiol (E2; 1 nmol/L) for 24 hours. miRNA-gene interactions were computationally predicted using Ingenuity Pathway Analysis and changes in miRNA levels were validated by qRT-PCR. Role of ER in the E2-induced miRNA was additionally confirmed by using specific ER agonists and antagonists. Results: miRNA array revealed that expression of 114 miRNA were significantly modified after E2 exposition. Further biological pathway analysis revealed cell death and survival, lipid metabolism, reproductive system function, as the top functions regulated by E2. We validated changes in the most significantly increased (miR-30b-5p, miR-487a-5p, miR-4710, miR-501-3p) and decreased (miR-378h and miR-1244) miRNA and the role of ER in these E2-induced miRNA was determined. Results showed that both classical, ERα and ERβ, and membrane-bound ER, GPER, differentially regulated specific miRNA. In silico analysis of validated miRNA promoters identified specific ER binding sites. Conclusion: Our findings identify differentially expressed miRNA pathways linked to E2 in human endothelial cells through ER, and provide new insights by which estrogen can modulate endothelial function

Regulatory network analysis in estradiol-treated human endothelial cells.

Background/Aims: Estrogen has been reported to have beneficial effects on vascular biology through direct actions on endothelium. Together with transcription factors, miRNAs are the major drivers of gene expression and signaling networks. The objective of this study was to identify a com-prehensive regulatory network (miRNA-transcription factor-downstream genes) that controls the transcriptomic changes observed in endothelial cells exposed to estradiol. Methods: miR-NA/mRNA interactions were assembled using our previous microarray data of human umbilical vein endothelial cells (HUVEC) treated with 17ß- Estradiol (E2) (1 nmol/lL, 24 h). miRNA--mRNA pairings and their associated canonical pathways were determined using Ingenuity Pathway Analysis software. Transcription factors were identified among the miR-NA-regulated genes. Transcription factor downstream target genes were predicted by consensus transcription factor binding sites in the promoter region of E2-regulated genes by using JASPAR and TRANSFAC tools in Enrichr software. Results: miRNA--target pairings were filtered by using differentially expressed miRNAs and mRNAs characterized by a regulatory relationship accord-ing to miRNA target prediction databases. The analysis identified 588 miRNA--target interactions between 102 miRNAs and 588 targets. Specifically, 63 up-regregulated miRNAs interacted with 295 down-regregulated targets, while 39 down-regregulated miRNAs were paired with 293 up-regregulated mRNA targets. Functional characterization of miRNA/mRNA association analy-sis highlighted hypoxia signallignaling, integrin, ephrin receptor signaling, and regulation of actin-based motility by Rho among the canonical pathways regulated by E2 in HUVEC. Tran-scription factors and downstream genes analysis revealed a total of eight networks, including those mediated by JUN and REPIN1, which are associated with cadherin binding and cell adhe-sion molecule binding pathways. Conclusion: This study identifies regulatory networks obtained by integrative microarray analysis and provides additional insights into the way estradiol could regulate endothelial function in human endothelial cells