Fecal Bacteria in Agricultural Waters of the Bluegrass Region of Kentucky

Agricultural runoff influenced by nonpoint pollution frequently exceeds the USEPA standards for bacterial contamination of primary contact water (200 fecal coliforms/100 mL). Few studies have evaluated the effect of cattle (Bos taurus) grazing on fecal contamination of ground water in the karst topography of central Kentucky. Our objectives were to: (i) observe the extent and pattern of fecal bacteria in agricultural waters from two central Kentucky watersheds; (ii) determine if monthly sampling accurately assessed the extent and variability of fecal contamination; and (iii) assess the fecal coliform/fecal streptococci ratio (FC/FS) as an indicator of fecal bacteria source. Springs, streams, and wells in two agricultural watersheds typical of central Kentucky were monitored for fecal coliform and fecal streptococci from December 1991 to January 1993. Springs and wells exceeded primary contact water standards, between 28 and 74% of the time; streams exceeded water quality standards between 87 and 100% of the time. When fecal bacteria were present, rainfall rapidly moved them from the soil surface into spring and well water. At two springs in Fleming county, only 29% of samples exceeded primary contact standards before cattle were present; 80% exceeded standards after cattle began grazing the surrounding pasture. Monthly sampling adequately reflected the extent of fecal contamination in our study, which had relatively continuous cattle grazing. Although the FC/FS ratio identified domestic animal contamination sources, it did not distinguish between domestic animal and human sources of contamination

Particle Size and Temperature Affect Fecal Bacteria Survival in Sediment

When cattle have direct access to streams, fecal bacteria concentrations in stream sediments increase. If these bacteria persist, and if the sediments are resuspended, fecal bacteria may also appear in surrounding water for extended periods. Why do fecal bacteria persist, since dry conditions, high acidity or alkalinity, sunlight, competition from native microbes, and extreme temperatures all diminish their populations in soil? The effects of these environmental factors are much reduced in sediment. Water protects fecal bacteria from desiccation and ultraviolet light. High temperatures can promote their regrowth in wet environments. Fecal bacteria also survive on fine-sized sediments in streams because the sediments have a high surface area. These factors may help explain our observations that streams flowing through pastures typically exceed Kentucky standards for primary contact water (200 fecal coliforms/100 ml) long after cattle depart. The fecal coliform/fecal streptococci ratio (FC/FS), is a tool in water quality assessment that diagnoses the source of fecal contamination, whether from people (FC/FS \u3e 4) or animals (FC/FS \u3c 0.1). The ratio is extremely variable and sensitive to the persistence of the indicator bacteria used in it. For example, we observed in central Kentucky streams that as the temperature increased during spring, the FC/FS ratio also increased. Fecal coliform growth shortly after manure deposition might explain some of the variability we have observed in our water monitoring studies. In this study we tried to account for the seasonal variability of FC/FS ratios in agricultural watersheds, and determine whether sediment particle size and water temperature interacted to influence fecal bacteria persistence and the FC/FS ratio

Effect of Sediment Particle Size and Temperature on Fecal Bacteria Mortality Rates and the Fecal Coliform/Fecal Streptococci Ratio

Extended survival of fecal bacteria in sediment can obscure the source and extent of fecal contamination in agricultural settings. The variability in fecal coliform/fecal streptococci (FC/FS) ratios with time and discrepancies between observable fecal sources and measured FC/FS ratios in shallow surface water from agricultural watersheds may be explained by examining FC and FS mortality rates in response to ambient temperature and sediment particle size. We measured FC and FS mortality rates at three different temperatures and in three feces-amended sediments with different particle size in a laboratory study. In controlled conditions, using physiological saline to reduce cell death by osmotic shock, FC mortality rates exceeded FS mortality rates. These rates declined as sediment particle-size shrank and as temperature decreased. There was no interaction between these two factors in determining fecal bacteria persistence. The apparent half-lives of FCs exceeded those of FS, even though mortality rates were higher, because of FC regrowth shortly after deposition. The FC/FS ratio is influenced by temperature, the presence of sediment, and sediment particle size. In warm conditions, FC regrowth increases FC/FS ratios to levels indicative of human contamination even where none clearly exists. These factors interfere with the interpretation of the FC/FS ratio and contribute to the difficulty of its use in agricultural settings

Fecal Bacteria in Agricultural Waters of the Bluegrass Region of Kentucky

Agricultural runoff influenced by nonpoint pollution frequently exceeds the USEPA standards for bacterial contamination of primary contact water (200 fecal coliforms/100 mL). Few studies have evaluated the effect of cattle (Bos taurus) grazing on fecal contamination of ground water in the karst topography of central Kentucky. Our objectives were to: (i) observe the extent and pattern of fecal bacteria in agricultural waters from two central Kentucky watersheds; (ii) determine if monthly sampling accurately assessed the extent and variability of fecal contamination; and (iii) assess the fecal coliform/fecal streptococci ratio (FC/FS) as an indicator of fecal bacteria source. Springs, streams, and wells in two agricultural watersheds typical of central Kentucky were monitored for fecal coliform and fecal streptococci from December 1991 to January 1993. Springs and wells exceeded primary contact water standards, between 28 and 74% of the time; streams exceeded water quality standards between 87 and 100% of the time. When fecal bacteria were present, rainfall rapidly moved them from the soil surface into spring and well water. At two springs in Fleming county, only 29% of samples exceeded primary contact standards before cattle were present; 80% exceeded standards after cattle began grazing the surrounding pasture. Monthly sampling adequately reflected the extent of fecal contamination in our study, which had relatively continuous cattle grazing. Although the FC/FS ratio identified domestic animal contamination sources, it did not distinguish between domestic animal and human sources of contamination

An assessment of the hopping strategy and inter-limb asymmetry during the triple hop test: a test–retest pilot study

The aims of the present study are to: (1) determine within- and between-session reliability of multiple metrics obtained during the triple hop test; and (2) determine any systematic bias in both the test and inter-limb asymmetry scores for these metrics. Thirteen male young American football athletes performed three trials of a triple hop test on each leg on two separate occasions. In addition to the total distance hopped, manual detection of touch down and toe-off were calculated via video analysis, enabling flight time (for each hop), ground contact time (GCT), reactive strength index (RSI), and leg stiffness (between hops) to be calculated. Results showed all coefficient of variation (CV) values were ≤ 10.67% and intraclass correlation coefficients (ICC) ranged from moderate to excellent (0.53–0.95) in both test sessions. Intrarater reliability showed excellent reliability for all metrics (CV ≤ 3.60%, ICC ≥ 0.97). No systematic bias was evident between test sessions for raw test scores (g = −0.34 to 0.32) or the magnitude of asymmetry (g = −0.19 to 0.43). However, ‘real’ changes in asymmetry (i.e., greater than the CV in session 1) were evident on an individual level for all metrics. For the direction of asymmetry, kappa coefficients revealed poor-to-fair levels of agreement between test sessions for all metrics (K = −0.10 to 0.39), with the exception of the first hop (K = 0.69). These data show that, given the inherent limitations of distance jumped in the triple hop test, practitioners can confidently gather a range of reliable data when computed manually, provided sufficient test familiarization is conducted. In addition, although the magnitude of asymmetry appears to show only small changes between test sessions, limb dominance does appear to fluctuate between test sessions, highlighting the value of also monitoring the direction of the imbalance

Recent Decisions

Comments on recent decisions by Norman H. McNeil, David N. McBride, Robert J. Hepler, John P. Coyne, and Allan Schmid

A Search for Intrinsic Polarization in O Stars with Variable Winds

New observations of 9 of the brightest northern O stars have been made with the Breger polarimeter on the 0.9~m telescope at McDonald Observatory and the AnyPol polarimeter on the 0.4~m telescope at Limber Observatory, using the Johnson-Cousins UBVRI broadband filter system. Comparison with earlier measurements shows no clearly defined long-term polarization variability. For all 9 stars the wavelength dependence of the degree of polarization in the optical range can be fit by a normal interstellar polarization law. The polarization position angles are practically constant with wavelength and are consistent with those of neighboring stars. Thus the simplest conclusion is that the polarization of all the program stars is primarily interstellar. The O stars chosen for this study are generally known from ultraviolet and optical spectroscopy to have substantial mass loss rates and variable winds, as well as occasional circumstellar emission. Their lack of intrinsic polarization in comparison with the similar Be stars may be explained by the dominance of radiation as a wind driving force due to higher luminosity, which results in lower density and less rotational flattening in the electron scattering inner envelopes where the polarization is produced. However, time series of polarization measurements taken simultaneously with H-alpha and UV spectroscopy during several coordinated multiwavelength campaigns suggest two cases of possible small-amplitude, periodic short-term polarization variability, and therefore intrinsic polarization, which may be correlated with the more widely recognized spectroscopic variations.Comment: LaTeX2e, 22 pages including 11 tables; 12 separate gif figures; uses aastex.cls preprint package; accepted by The Astronomical Journa

Developing social capital in implementing a complex intervention: a process evaluation of the early implementation of a suicide prevention intervention in four European countries

<p>Background: Variation in the implementation of complex multilevel interventions can impact on their delivery and outcomes. Few suicide prevention interventions, especially multilevel interventions, have included evaluation of both the process of implementation as well as outcomes. Such evaluation is essential for the replication of interventions, for interpreting and understanding outcomes, and for improving implementation science. This paper reports on a process evaluation of the early implementation stage of an optimised suicide prevention programme (OSPI-Europe) implemented in four European countries.</p> <p>Methods: The process analysis was conducted within the framework of a realist evaluation methodology, and involved case studies of the process of implementation in four European countries. Datasets include: repeated questionnaires to track progress of implementation including delivery of individual activities and their intensity; serial interviews and focus groups with stakeholder groups; and detailed observations at OSPI implementation team meetings.</p> <p>Results: Analysis of local contexts in each of the four countries revealed that the advisory group was a key mechanism that had a substantial impact on the ease of implementation of OSPI interventions, particularly on their ability to recruit to training interventions. However, simply recruiting representatives of key organisations into an advisory group is not sufficient to achieve impact on the delivery of interventions. In order to maximise the potential of high level ‘gatekeepers’, it is necessary to first transform them into OSPI stakeholders. Motivations for OSPI participation as a stakeholder included: personal affinity with the shared goals and target groups within OSPI; the complementary and participatory nature of OSPI that adds value to pre-existing suicide prevention initiatives; and reciprocal reward for participants through access to the extended network capacity that organisations could accrue for themselves and their organisations from participation in OSPI.</p> <p>Conclusions: Exploring the role of advisory groups and the meaning of participation for these participants revealed some key areas for best practice in implementation: careful planning of the composition of the advisory group to access target groups; the importance of establishing common goals; the importance of acknowledging and complementing existing experience and activity; and facilitating an equivalence of benefit from network participation.</p&gt

Abuse in the caregiving relationship between older people with memory disorders and family caregivers: A systematic review

Aim To synthesize what is known about elder abuse and relationship factors associated with abuse between caregivers and older people with memory disorders at home. Background Concerns about abuse in the caring relationship between older people with memory disorders and family caregivers have increased. Abuse is associated with negative outcomes on older people's health, quality of life, and zest for life. Abuse in the caring relationship manifests in financial exploitation, neglect, mistreatment, and physical issues. Design Systematic review. Data Sources Databases including Scopus, PubMed/Medline, SveMed+, Cinalh, SonINDEX, and ProQuest were searched using keywords about abuse in the caring relationship between older people with memory disorders and family caregivers at home. Articles published between 2005–2019 were retrieved and underwent data analysis and knowledge synthesis. Review Methods The review was presented under the categories of the dyadic approach of elder abuse in connection with the role of caregiver (risk) and care recipient (vulnerability) by Fulmer et al. (2005). Results The search process led to 12 quantitative studies, including an intervention, a prospective, nine surveys, and a cross‐sectional structural interview. Findings were synthesized and presented under ‘personal’, ‘physical and psychological’, and ‘social’ domains indicating the bilateral roles of caregiver and care recipient leading to abuse. Conclusion This review depicted factors influencing abuse in the caring relationship between older people with memory disorders and their family caregivers at home. They included family caregivers’ psychological issues, knowledge of memory disorders and modifications, previous caring relationship, social support, number of care recipients, and care recipients’ functional level. Impact This review identifies what influences elder abuse by family caregivers using the dyadic approach and explains how abuse can be prevented through suggested strategies. The review findings are relevant to multidisciplinary healthcare providers and can guide the provision of support, screening and assessment, educational programs, and legislative initiatives