Análisis socio-jurídico del comportamiento delictivo por parte de los adolescentes en el municipio de San José de Cúcuta, y su sanción en los años 2011-2014

La investigación sobre el análisis socio-jurídico del comportamiento delictivo por parte de los adolescentes en el municipio de San José de Cúcuta, y su sanción en los años 2011-2014, la cual busca identificar los delitos cometidos por los adolescentes que han ingresado al Sistema de Responsabilidad Penal en el Municipio de San José de Cúcuta; así como describir las sanciones que han sido impuestas a los adolescentes responsables de la comisión de delitos, de acuerdo al Sistema de Responsabilidad Penal en el Municipio de San José de Cúcuta; caracterizar los factores que han incidido en la reincidencia de los adolescentes en la comisión de delitos en el Municipio de San José de Cúcuta en los años 2011-2014; se origina en la necesidad de examinar a qué se atribuye el aumento de la actividad delictiva por parte de este grupo poblacional, así como la reincidencia de éstos en este tipo de conductas. Se trata de un análisis mixto, es decir que conjuga los métodos cualitativo y cuantitativo, mediante el estudio de documentos y estadísticas, para llegar a describir la problemática de los adolescentes implicados en actividades delictivas, basados en las cifras del Sistema de Responsabilidad Penal Adolescente, que muestran un notable aumento en estos años 2011-2014, y que además evidencian la reincidencia de los adolescentes en la comisión de delitos.Research on the socio-legal analysis of criminal behavior by adolescents in the municipality of San José de Cúcuta, and its sanction in the years 2011-2014, which seeks to identify the crimes committed by adolescents who have entered the System Criminal Responsibility in the Municipality of San José de Cúcuta; as well as describe the sanctions that have been imposed on adolescents responsible for committing crimes, according to the System of Criminal Responsibility in the Municipality of San José de Cúcuta; characterize the factors that have influenced the recidivism of adolescents in the commission of crimes in the Municipality of San José de Cúcuta in the years 2011-2014; It originates from the need to examine what is attributed to the increase in criminal activity by this population group, as well as their recidivism in this type of behavior. It is a mixed analysis, that is, it combines qualitative and quantitative methods, through the study of documents and statistics, to describe the problems of adolescents involved in criminal activities, based on the figures of the Adolescent Criminal Responsibility System, that show a notable increase in these years 2011-2014, and that also show the recidivism of adolescents in the commission of crimes

Polysomy of chromosome 17 in breast cancer tumors showing an overexpression of ERBB2: a study of 175 cases using fluorescence in situ hybridization and immunohistochemistry

INTRODUCTION: One of the most common genetic aberrations associated with breast cancer is the amplification and overexpression of the ERBB2 proto-oncogene located at chromosome 17, bands q12-21. The amplification/overexpression occurs in 25 to 30% of all breast cancers. In breast cancer, aneusomy of chromosome 17, either monosomy or polysomy, is frequently observed by conventional cytogenetics and fluorescence in situ hybridization (FISH). The aim of this study was to discover whether or not numerical aberrations on chromosome 17 have a correlation to the amplification or overexpression of the ERBB2 gene and to analyze their clinical implications in subgroups showing 2+ or 3+ positive scores by immunohistochemistry (IHC). METHODS: We used FISH on a series of 175 formalin-fixed paraffin-embedded breast carcinomas to detect ERBB2 amplification, using a dual-probe system for the simultaneous enumeration of the ERBB2 gene and the centromeric region of chromosome 17, as well as using IHC to detect overexpression. We analyzed clinical and pathological variables in a subgroup of patients with 2+ and 3+ IHC scores (147 patients), to describe any differences in clinicopathological characteristics between polysomic and non-polysomic cases with the use of the χ(2 )test. RESULTS: We found 13% of cases presenting polysomy, and three cases presented monosomy 17 (2%). According to the status of the ERBB2 gene, instances of polysomy 17 were more frequently observed in non-amplified cases than in FISH-amplified cases, suggesting that the mechanism for ERBB2 amplification is independent of polysomy 17. Polysomy 17 was detected in patients with 2+ and 3+ IHC scores. We found that nodal involvement was more frequent in polysomic than in non-polysomic cases (P = 0.046). CONCLUSIONS: The determination of the copy number of chromosome 17 should be incorporated into the assesment of ERBB2 status. It might also be helpful to differentiate a subgroup of breast cancer patients with polysomy of chromosome 17 and overexpression of ERBB2 protein that probably have genetic and clinical differences

FISH and immunohistochemical status of the hepatocyte growth factor receptor (c-Met) in 184 invasive breast tumors

Grants PI05/0961 and PI06/1513 from Ministerio de Sanidad y Consumo ISCIII and RTICC 06/0020/1

Preliminary design of next generation Mach 1.6 supersonic business jets to investigate landing & take-off (LTO) noise and emissions–SENECA

With the approach of next generation supersonic transport entry into service, new research activities were initiated to support updates on ICAO regulations and certification processes for supersonic transport vehicles. Within this context, the EU Horizon 2020 SENECA project has been launched to investigate the levels of noise and gaseous emissions in the vicinity of airports as well as the global climate impact of next generation supersonic civil aircraft. This paper introduces some of the preliminary outcomes of this investigation. It presents the preliminary design and performance analysis of a Mach 1.6 business jet, following an integrated aircraft-engine design approach. The preliminary design was performed accounting for the limitations posed by future environmental restrictions on respective subsonic vehicles. The market space and mission route definition exercise assumed only "over-sea" supersonic operations, while for "over-land", only subsonic operations where allowed. Parametric studies on engine integrated design demonstrated modest core temperatures while cruising and the significant impact of engine installation on performance. At this first design iteration, assuming current state of the art technology, the Mach 1.6 business jet showed good potential to satisfy the predicted mission requirements while respecting the environmental constraints in terms of Landing & Take-Off (LTO) noise and emissions

La Recreación en las Políticas Públicas. Entre la subordinación y la interrelación con otros campos.

El proyecto de investigación “Políticas Públicas de Recreación en la Provincia de Córdoba”, surge de la cátedra Gestión y Planeamiento de Políticas en Educación Física, Deporte y Recreación de la Licenciatura en Educación Física de la Universidad Provincial de Córdoba.A los fines de acompañar el desarrollo de trabajos finales de Licenciatura que tienen por objeto el análisis de programas de políticas públicas de Recreación, este proyecto pretende cubrir una vacancia en el estudio de esta temática en la provincia de Córdoba.En el presente artículo nos proponemos presentar los antecedentes, problemas, objetivos y metodología, para el estudio de políticas públicas recreativas, a partir de tres indagaciones exploratorias en curso.En una primera etapa, acercaremos nuestra mirada a programas socioeducativos y políticas recreativasn y deportivas que se desarrollan en diversos contextos y localidades de la provincia de Córdoba; a través de entrevistas semiestructuradas, observaciones no participantes y fuentes secundarias (documentos degestión, normativas, presupuestos).Esperamos que este proyecto de investigación, y el equipo de reciente formación que integramos, signifiquen un aporte al campo, así como también brinden algunas pistas para la formulación de políticas públicas encaminadas a reconocer y garantizar aquellos derechos vinculados al acceso y disfrute de prácticas recreativas

Enhancement of the HIV-1-Specific Immune Response Induced by an mRNA Vaccine through Boosting with a Poxvirus MVA Vector Expressing the Same Antigen

Development of a vaccine against HIV remains a major target goal in the field. The recent success of mRNA vaccines against the coronavirus SARS-CoV-2 is pointing out a new era of vaccine designs against pathogens. Here, we have generated two types of mRNA vaccine candidates against HIV-1; one based on unmodified vectors and the other on 1-methyl-3′-pseudouridylyl modified vectors expressing a T cell multiepitopic construct including protective conserved epitopes from HIV-1 Gag, Pol and Nef proteins (referred to as RNA-TMEP and RNA-TMEPmod, respectively) and defined their biological and immunological properties in cultured cells and in mice. In cultured cells, both mRNA vectors expressed the corresponding protein, with higher levels observed in the unmodified mRNA, leading to activated macrophages with differential induction of innate immune molecules. In mice, intranodal administration of the mRNAs induced the activation of specific T cell (CD4 and CD8) responses, and the levels were markedly enhanced after a booster immunization with the poxvirus vector MVA-TMEP expressing the same antigen. This immune activation was maintained even three months later. These findings revealed a potent combined immunization regimen able to enhance the HIV-1-specific immune responses induced by an mRNA vaccine that might be applicable to human vaccination programs with mRNA and MVA vectors.This study was partially supported by grants from the Spanish Ministry of Economy (MINECO) (grants: SAF2015-66193-R, SAF-2017-88089-R, RTI2018-096309-B-I00); the Fondo Europeo para el Desarrollo Regional (FEDER); the SPANISH AIDS Research Network RD16/0025/0002 and RD16/0025/0014-ISCIII-FEDER (RIS); the Fondo de Investigación Sanitaria (FIS) AC16/00051 and PI18/00699; the Instituto de Salud Carlos III (grants: COV20/00214; ICI20/00067) and the CERCA Programme/Generalitat de Catalunya SGR 615 and SGR 653. This manuscript was funded by the European Commission [grant numbers: FP7-HEALTH-2013-INNOVATION-1 602570-2, H2020-SC1- 2016-2017 (H2020-SC1-2016-RTD) Proposal: 731626-HIVACAR].Peer reviewe

Índice de desempeño ambiental del territorio

El modelo que subyace a los índices de desempeño ambiental están circunscrito a variables que desarrollen medidas de evaluación del estado de cada uno de las dimensiones, las presiones a las que estas dimensiones han venido siendo sometidas y las respuestas que desde la gobernanza de los diversos actores que interactúan, pretenden o disminuir las presiones o disminuir la vulnerabilidad de los elementos que componen las dimensiones social, económica y ecológica.Bogotá D.C

SoxD genes are required for adult neural stem cell activation

19 páginas, 6 figuras. Supplemental information can be found online at https://doi.org/10.1016/j. celrep.2022.110313.The adult neurogenic niche in the hippocampus is maintained through activation of reversibly quiescent neural stem cells (NSCs) with radial glia-like morphology (RGLs). Here, we show that the expression of SoxD transcription factors Sox5 and Sox6 is enriched in activated RGLs. Using inducible deletion of Sox5 or Sox6 in the adult mouse brain, we show that both genes are required for RGL activation and the generation of new neurons. Conversely, Sox5 overexpression in cultured NSCs interferes with entry in quiescence. Mechanistically, expression of the proneural protein Ascl1 (a key RGL regulator) is severely downregulated in SoxD-deficient RGLs, and Ascl1 transcription relies on conserved Sox motifs. Additionally, loss of Sox5 hinders the RGL activation driven by neurogenic stimuli such as environmental enrichment. Altogether, our data suggest that SoxD genes are key mediators in the transition of adult RGLs from quiescence to an activated mitotic state under physiological situations.This work was funded by grants to A.V.M. from the Spanish MICINN (SAF2017-85717-R, PID2020-112989RB-I00) and F. Alicia Koplowitz (2018) and to H.M. from the Spanish MICINN (SAF2015-70433-R, PID2019- 111225RB-I00) and PROMETEO/2018/055 from Generalitat ValencianaPeer reviewe

Gene Therapy Corrects Mitochondrial Dysfunction in Hematopoietic Progenitor Cells and Fibroblasts from Coq9R239X Mice

This study has been submitted to the patent's offices at the "University of Granada" and "Fundación Progreso y Salud". Please note that the results of this manuscript have been submitted to patent protection (application number P201630630; title: “Uses of Coenzyme Q biosynthetic proteins”; date:05/16/2016).Recent clinical trials have shown that in vivo and ex vivo gene therapy strategies can be an option for the treatment of several neurological disorders. Both strategies require efficient and safe vectors to 1) deliver the therapeutic gene directly into the CNS or 2) to genetically modify stem cells that will be used as Trojan horses for the systemic delivery of the therapeutic protein. A group of target diseases for these therapeutic strategies are mitochondrial encephalopathies due to mutations in nuclear DNA genes. In this study, we have developed a lentiviral vector (CCoq9WP) able to overexpress Coq9 mRNA and COQ9 protein in mouse embryonic fibroblasts (MEFs) and hematopoietic progenitor cells (HPCs) from Coq9R239X mice, an animal model of mitochondrial encephalopathy due to primary Coenzyme Q (CoQ) deficiency. Ectopic over-expression of Coq9 in both cell types restored the CoQ biosynthetic pathway and mitochondrial function, improving the fitness of the transduced cells. These results show the potential of the CCoq9WP lentiviral vector as a tool for gene therapy to treat mitochondrial encephalopathies.This work was supported by grants from Ministerio de Economía y Competitividad (Spain) and the European Regional Development Fund (ERDF) from the European Union, to LCL through the research grants SAF2013-47761-R and SAF2015-65786-R; by Fondo de Investigaciones Sanitarias ISCIII (Spain) and the European Regional Development Fund (ERDF) from the European Union through the research grants PI12/01097 and ISCIII Red de Terapia Celular TerCel RD12/0019/0006 to FM; by the Consejería de Economía, Innovación, Ciencia y Empleo, Junta de Andalucía-FEDER/Fondo de Cohesion Europeo (FSE) de Andalucía through the research grants P10-CTS-6133 to LCL; P09-CTS-04532, PI-57069, PI-0001/2009 and PAIDI-Bio-326 to F.M.; PI-0160/2012 to KB and PI-0407/2012 to MC; by the NIH through the research P01HD080642 to LCL and by the foundation “todos somos raros, todos somos únicos” to LCL. LCL is supported by the ‘Ramón y Cajal’ National Programme, Ministerio de Economía y Competitividad, Spain (RYC-2011-07643)

Seven-versus 14-day course of antibiotics for the treatment of bloodstream infections by Enterobacterales: a randomized, controlled trial

[Objective] To prove that 7-day courses of antibiotics for bloodstream infections caused by members of the Enterobacterales (eBSIs) allow a reduction in patients' exposure to antibiotics while achieving clinical outcomes similar to those of 14-day schemes.[Methods] A randomized trial was performed. Adult patients developing eBSI with appropriate source control were assigned to 7 or 14 days of treatment, and followed 28 days after treatment cessation; treatments could be resumed whenever necessary. The primary endpoint was days of treatment at the end of follow-up. Clinical outcomes included clinical cure, relapse of eBSI and relapse of fever. A superiority margin of 3 days was set for the primary endpoint, and a non-inferiority margin of 10% was set for clinical outcomes. Efficacy and safety were assessed together with a DOOR/RADAR (desirability of outcome ranking and response adjusted for duration of antibiotic risk) analysis.[Results] 248 patients were assigned to 7 (n = 119) or 14 (n = 129) days of treatment. In the intention-to-treat analysis, median days of treatment at the end of follow-up were 7 and 14 days (difference 7, 95%CI 7–7). The non-inferiority margin was also met for clinical outcomes, except for relapse of fever (–0.2%, 95%CI –10.4 to 10.1). The DOOR/RADAR showed that 7-day schemes had a 77.7% probability of achieving better results than 14-day treatments.[Conclusions] 7-day schemes allowed a reduction in antibiotic exposure of patients with eBSI while achieving outcomes similar to those of 14-day schemes. The possibility of relapsing fever in a limited number of patients, without relevance to final outcomes, may not be excluded, but was overcome by the benefits of shortening treatments.This work received technical support from the Spanish Network of Research in Infectious Diseases (REIPI) and the Spanish Clinical Research Network (SCReN) of the Instituto de Salud Carlos III, Ministerio de Economía y Competitividad, Spanish Government (PT13/0002/0010).Peer reviewe