Immobilization of an Artificial Imine Reductase within Silica Nanoparticles Improves its Performance

Silica nanoparticles equipped with an artificial imine reductase display remarkable activity towards cyclic imine- and NAD + reduction. The method, based on immobilization and protection of streptavidin on silica nanoparticles, shields the biotinylated metal cofactor against deactivation yielding over 46 000 turnovers in pure samples and 4000 turnovers in crude cellular extracts

Управление затратами как эффективный инструмент выхода предприятия из кризиса и альтернатива "экономии на людях"

В данной работе рассмотрен механизм последовательного повышения цен среди предприятий во время кризиса, обозначена склонность некоторых предприятий необдуманно сокращать затраты на персонал, обоснована необходимость использовать управление затратами как более эффективную альтернативу традиционной экономии затрат, рассмотрено понятие управления затратами как деятельность предприятия и набор методик, перечислены основные виды затрат и способы их оптимизации

Biodegradation of mixture of plastic films by tailored marine consortia

Summarization: This work sheds light on the physicochemical changes of naturally weathered polymer surfaces along with changes of polymer buoyancy due to biofilm formation and degradation processes. To support the degradation hypothesis, a microcosm experiment was conducted where a mixture of naturally weathered plastic pieces was incubated with an indigenous pelagic community. A series of analyses were employed in order to describe the alteration of the physicochemical characteristics of the polymer (FTIR, SEC and GPC, sinking velocity)as well as the biofilm community (NGS). At the end of phase II, the fraction of double bonds in the surface of microbially treated PE films increased while changes were also observed in the profile of the PS films. The molecular weight of PE pieces increased with incubation time reaching the molecular weight of the virgin pieces (230,000 g mol−1)at month 5 but the buoyancy displayed no difference throughout the experimental period. The number-average molecular weight of PS pieces decreased (33% and 27% in INDG and BIOG treatment respectively), implying chain scission; accelerated (by more than 30%)sinking velocities compared to the initial weathered pieces were also measured for PS films with biofilm on their surface. The orders Rhodobacterales, Oceanospirillales and Burkholderiales dominated the distinct platisphere communities and the genera Bacillus and Pseudonocardia discriminate these assemblages from the planktonic counterpart. The functional analysis predicts overrepresentation of adhesive cells carrying xenobiotic and hydrocarbon degradation genes. Taking these into account, we can suggest that tailored marine consortia have the ability to thrive in the presence of mixtures of plastics and participate in their degradation. Παρουσιάστηκε στο: Journal of Hazardous Material

Технологические решения для строительства разведочной вертикальной скважины глубиной 2950 метров на нефтяном месторождении (Тюменская область)

Цель работы – проектирование вертикальной разведочной скважины глубиной 2950 метров. В процессе работы был составлен проект на строительство вертикальной разведочной скважины на нефть глубиной 2950 м (по вертикали). Разработаны мероприятия по организации строительству, охране труда и окружающей среды. В работе рассмотрен вопрос технология установки клино-отклонителя.The purpose of the work is to design a vertical exploration well with a depth of 2,950 meters. In the process, a project was drawn up for the construction of a vertical exploration well for oil of 2950 m depth (vertical). Developed measures for the organization of construction, labor and environmental protection. The paper considers the issue of installation technology wedge-diverter

Investigation by thermodesorption spectroscopy of hydrogen accumulation features in Zr-1%Nb zirconium alloy during gas-phase hydrogenation

Modern trends in human development require more and more electricity to maintain the pace of economic and scientific development. One of the most environmentally friendly methods of electricity production is the use of nuclear power plants (NPPs). In connection with high impact on structural materials of nuclear power plants cores there is uncontrolled degradation of core material. Understanding the degradation processes will help predict and prevent various man-made disasters. The purpose of this work is to study the accumulation of hydrogen in the zirconium alloy Zr-1%Nb (E110). In this work, we studied the processes of hydrogen sorption and desorption by the zirconium alloy Zr-1%Nb

State-of-the-art production chains for peas, beans and chickpeas\u2014valorization of agro-industrial residues and applications of derived extracts

The world is confronted with the depletion of natural resources due to their unsustainable use and the increasing size of populations. In this context, the efficient use of by-products, residues and wastes generated from agro-industrial and food processing opens the perspective for a wide range of benefits. In particular, legume residues are produced yearly in very large amounts and may represent an interesting source of plant proteins that contribute to satisfying the steadily increasing global protein demand. Innovative biorefinery extraction cascades may also enable the recovery of further bioactive molecules and fibers from these insufficiently tapped biomass streams. This review article gives a summary of the potential for the valorization of legume residual streams resulting from agro-industrial processing and more particularly for pea, green bean and chickpea by-products/wastes. Valuable information on the annual production volumes, geographical origin and state-of-the-art technologies for the extraction of proteins, fibers and other bioactive molecules from this source of biomass, is exhaustively listed and discussed. Finally, promising applications, already using the recovered fractions from pea, bean and chickpea residues for the formulation of feed, food, cosmetic and packaging products, are listed and discussed

Aerobic nonylphenol degradation and nitro-nonylphenol formation by microbial cultures from sediments

Nonylphenol (NP) is an estrogenic pollutant which is widely present in the aquatic environment. Biodegradation of NP can reduce the toxicological risk. In this study, aerobic biodegradation of NP in river sediment was investigated. The sediment used for the microcosm experiments was aged polluted with NP. The biodegradation of NP in the sediment occurred within 8 days with a lag phase of 2 days at 30°C. During the biodegradation, nitro-nonylphenol metabolites were formed, which were further degraded to unknown compounds. The attached nitro-group originated from the ammonium in the medium. Five subsequent transfers were performed from original sediment and yielded a final stable population. In this NP-degrading culture, the microorganisms possibly involved in the biotransformation of NP to nitro-nonylphenol were related to ammonium-oxidizing bacteria. Besides the degradation of NP via nitro-nonylphenol, bacteria related to phenol-degrading species, which degrade phenol via ring cleavage, are abundantly present