Red (660 nm) and infrared (830 nm) low-level laser therapy in skeletal muscle fatigue in humans: what is better?

In animal and clinical trials low-level laser therapy (LLLT) using red, infrared and mixed wavelengths has been shown to delay the development of skeletal muscle fatigue. However, the parameters employed in these studies do not allow a conclusion as to which wavelength range is better in delaying the development of skeletal muscle fatigue. With this perspective in mind, we compared the effects of red and infrared LLLT on skeletal muscle fatigue. A randomized double-blind placebo-controlled crossover trial was performed in ten healthy male volunteers. They were treated with active red LLLT, active infrared LLLT (660 or 830 nm, 50 mW, 17.85 W/cm2, 100 s irradiation per point, 5 J, 1,785 J/cm2 at each point irradiated, total 20 J irradiated per muscle) or an identical placebo LLLT at four points of the biceps brachii muscle for 3 min before exercise (voluntary isometric elbow flexion for 60 s). The mean peak force was significantly greater (p < 0.05) following red (12.14%) and infrared LLLT (14.49%) than following placebo LLLT, and the mean average force was also significantly greater (p < 0.05) following red (13.09%) and infrared LLLT (13.24%) than following placebo LLLT. There were no significant differences in mean average force or mean peak force between red and infrared LLLT. We conclude that both red than infrared LLLT are effective in delaying the development skeletal muscle fatigue and in enhancement of skeletal muscle performance. Further studies are needed to identify the specific mechanisms through which each wavelength acts

Análise da postura craniocervical de crianças respiradoras bucais após tratamento postural em bola suíça

The study aimed to evaluate the craniocervical posture of mouth breathing children after postural treatment on swiss ball. Twelve mouth breathing children were undergone to a postural reeducation protocol through stretching and strengthening exercises on swiss ball, diaphragmatic stimulation and stretching of the inspiratory accessory muscles. Craniocervical posture was evaluated through biophotogrammetry analysis. Forward head position was measured through an angle formed by the points in the tragus and in the spinous process of the seventh cervical vertebra with a horizontal line. Cervical column curvature was taken by the horizontal distance from a vertical line passing through the thoracic kyphosis apex to the point of the greatest cervical curvature concavity. Pictures were taken before and after ten treatment sessions. The normality of the variables was tested by Shapiro-Wilk test and the Student's t -test was used to determine differences in variables between assessments. It was considered a significance level of 5% (pO estudo teve como objetivo avaliar a postura craniocervical de crianças respiradoras bucais após tratamento postural em bola suíça. Doze crianças respiradoras bucais foram submetidas a um protocolo de reeducação postural constituído por exercícios de alongamento e fortalecimento muscular sobre a bola suíça, estimulação diafragmática e alongamento dos músculos acessórios da inspiração. A postura craniocervical foi avaliada através da análise biofotogramétrica. A posição da anteriorização da cabeça foi aferida por meio do ângulo formado pelos pontos localizados no tragus direito e no processo espinhoso da sétima vértebra cervical com a linha horizontal. A curvatura cervical foi avaliada pela distância horizontal de uma linha vertical tangenciando o ápice da cifose torácica e o ponto de maior concavidade da curvatura cervical. As fotografias foram obtidas antes e após dez atendimentos. A normalidade das variáveis foi verificada a partir do teste Shapiro-Wilk. Para as comparações entre as médias foi utilizado o teste t de Student para amostras dependentes admitindo-se nível de significância de 5% (

Profile and motivation for smoking cessation in surgical inpatients

Estudo transversal que objetivou identifi car o perfi l e a motivação para a cessação do tabagismo em pacientes cirúrgicos hospitalizados. A amostra foi aleatória, com 100 pacientes de um hospital universitário do Sul do Brasil. Os dados foram coletados entre fevereiro e maio de 2013 e analisados pela estatística descritiva. Os resultados evidenciaram que 58 (58%) eram homens, com idade de 54,5±13,8 anos, 79(79%) brancos, 38(38%) casados e 67 (67%) com ensino fundamental. O início do fumo ocorreu aos 17±6,6 anos, com consumo de 20(10-28,7) cigarros/dia e com 37,4±14,4 anos de fumo. Noventa e um (91%) pacientes desejavam parar de fumar, 57(57%) estavam na fase de preparação, 36(36%) apresentavam baixa dependência de nicotina e 35(35%) receberam incentivos para cessar o tabagismo. Conclui-se que a internação é um momento propício para abordagem do tabagismo, no entanto, a equipe de saúde ainda não intervém de maneira efi caz e sistematizada.Estudio transversal a identifi car el perfi l y la motivación para dejar de fumar en pacientes quirúrgicos. La muestra fue aleatoria con 100 pacientes de un hospital universitario en el sur de Brasil. Los datos fueron recolectados entre febrero y mayo de 2013 y analizados mediante estadística descriptiva. Los resultados mostraron que el 58(58%) eran hombres, con edades de 54,5±13,8 años, 79(79 %) blancos, 38(38%) casados y 67(67%) con educación primaria. El inicio del consumo de tabaco fue a los 17±6,6 años, 20(10 a 28,7) cigarrillos/día y 37,4±14,4 años fumadores. Noventa y un (91%) pacientes querían dejar de fumar, 57(57%) se encontraban en la fase de preparación, 36(36%) tenían baja dependencia de la nicotina y 35(35%) recibieron incentivos para dejar de fumar. Llegamos a la conclusión de que la hospitalización es un buen momento para la aproximación, sin embargo, el equipo de salud todavía no interviene con efi cacia y de manera sistemática.The aim of this cross-sectional study was to describe the profi le of smokers hospitalized for surgery, and investigate their motivation to quit. The sample consisted of 100 patients recruited from a university hospital in southern Brazil. Data were collected between February and May 2013, and analyzed using descriptive statistics. The sample was predominantly male (n=58; 58%) and had a mean age of 54.5±13.8 years. Seventy-nine (79%) of the participants were white, 38(38%) were married and 67(67%) had primary education only. Patients started smoking at a mean age of 17±6.6 years, smoked approximately 20(10 to 28.7) cigarettes/day and had been smoking for a mean of 37.4±14.4 years. Ninety-one (91%) patients wanted to stop smoking, 57(57%) were in the preparation phase, 36(36%) had low nicotine dependence and 35(35%) had been encouraged to quit. We concluded that, although hospitalization is a good moment to address smoking cessation, health care professionals do not enact eff ective and systematic interventions in this regard

HPV16 Oncoproteins Induce MMPs/RECK-TIMP-2 Imbalance in Primary Keratinocytes: Possible Implications in Cervical Carcinogenesis

Cervical cancer is the third most common cancer in women worldwide. Persistent infection with high-risk HPV types, principally HPV16 and 18 is the main risk factor for the development of this malignancy. However, the onset of invasive tumor occurs many years after initial exposure in a minority of infected women. This suggests that other factors beyond viral infection are necessary for tumor establishment and progression. Tumor progression is characterized by an increase in secretion and activation of matrix metalloproteinases (MMPs) produced by either the tumor cells themselves or tumor-associated fibroblasts or macrophages. Increased MMPs expression, including MMP-2, MMP-9 and MT1-MMP, has been observed during cervical carcinoma progression. These proteins have been associated with degradation of ECM components, tumor invasion, metastasis and recurrence. However, few studies have evaluated the interplay between HPV infection and the expression and activity of MMPs and their regulators in cervical cancer. We analyzed the effect of HPV16 oncoproteins on the expression and activity of MMP-2, MMP-9, MT1-MMP, and their inhibitors TIMP-2 and RECK in cultures of human keratinocytes. We observed that E7 expression is associated with increased pro-MMP-9 activity in the epithelial component of organotypic cultures, while E6 and E7 oncoproteins co-expression down-regulates RECK and TIMP-2 levels in organotypic and monolayers cultures. Finally, a study conducted in human cervical tissues showed a decrease in RECK expression levels in precancer and cancer lesions. Our results indicate that HPV oncoproteins promote MMPs/RECK-TIMP-2 imbalance which may be involved in HPV-associated lesions outcome