4,674 research outputs found
Resonance assignments for latherin, a natural surfactant protein from horse sweat
Latherin is an intrinsically surfactant protein of ~23 kDa found in the sweat and saliva of horses. Its function is probably to enhance the translocation of sweat water from the skin to the surface of the pelt for evaporative cooling. Its role in saliva may be to enhance the wetting, softening and maceration of the dry, fibrous food for which equines are adapted. Latherin is unusual in its relatively high content of aliphatic amino acids (~25 % leucines) that might contribute to its surfactant properties. Latherin is related to the palate, lung, and nasal epithelium carcinoma-associated proteins (PLUNCs) of mammals, at least one of which is now known to exhibit similar surfactant activity to latherin. No structures of any PLUNC protein are currently available. 15N,13C-labelled recombinant latherin was produced in Escherichia coli, and essentially all of the resonances were assigned despite the signal overlap due to the preponderance of leucines. The most notable exceptions include a number of residues located in an apparently dynamic loop region between residues 145 and 154. The assignments have been deposited with BMRB accession number 19067
The structure of latherin, a surfactant allergen protein from horse sweat and saliva
Latherin is a highly surface-active allergen protein found in the sweat and saliva of horses and other equids. Its surfactant activity is intrinsic to the protein in its native form, and is manifest without associated lipids or glycosylation. Latherin probably functions as a wetting agent in evaporative cooling in horses, but it may also assist in mastication of fibrous food as well as inhibition of microbial biofilms. It is a member of the PLUNC family of proteins abundant in the oral cavity and saliva of mammals, one of which has also been shown to be a surfactant and capable of disrupting microbial biofilms. How these proteins work as surfactants while remaining soluble and cell membrane-compatible is not known. Nor have their structures previously been reported. We have used protein nuclear magnetic resonance spectroscopy to determine the conformation and dynamics of latherin in aqueous solution. The protein is a monomer in solution with a slightly curved cylindrical structure exhibiting a ‘super-roll’ motif comprising a four-stranded anti-parallel β-sheet and two opposing α-helices which twist along the long axis of the cylinder. One end of the molecule has prominent, flexible loops that contain a number of apolar amino acid side chains. This, together with previous biophysical observations, leads us to a plausible mechanism for surfactant activity in which the molecule is first localized to the non-polar interface via these loops, and then unfolds and flattens to expose its hydrophobic interior to the air or non-polar surface. Intrinsically surface-active proteins are relatively rare in nature, and this is the first structure of such a protein from mammals to be reported. Both its conformation and proposed method of action are different from other, non-mammalian surfactant proteins investigated so far
Shining a Light on s-Triazine-Based Polymers
The strong interplay between the structure and optical properties of conjugated s-triazine-based framework (CTF) materials is explored in a combined experimental and computational study. The experimental absorption and fluorescence spectra of the CTF-1 material, a polymer obtained through the trimerization of 1,4-dicyanobenzene, are compared with the results of time-dependent density functional theory and approximate coupled cluster theory (CC2) calculations on cluster models of the polymer. To help explain the polymer data, we compare its optical properties with those measured and predicted for the 2,4,6-triphenyl-1,3,5-triazine model compound. Our analysis shows that CTFs, in line with experimental diffraction data, are likely to be layered materials based around flat hexagonal-like sheets and suggests that the long-wavelength part of the CTF-1 absorption spectrum displays a pronounced effect of stacking. Red-shifted peaks in the absorption spectrum appear that are absent for an isolated sheet. We also show that the experimentally observed strong fluorescence of CTF-1 and other CTF materials is further evidence of the presence of rings in the layers, as structures without rings are predicted to have extremely long excited state lifetimes and hence would display negligible fluorescence intensities. Finally, subtle differences between the experimental absorption spectra of CTF-1 samples prepared using different synthesis routes are shown to potentially arise from different relative arrangements of stacked layers
The Evolution of Environmental Quenching Timescales to
Using a sample of 4 galaxy clusters at and 10 galaxy
clusters at , we measure the environmental quenching
timescale, , corresponding to the time required after a galaxy is accreted
by a cluster for it to fully cease star formation. Cluster members are selected
by a photometric-redshift criterion, and categorized as star-forming,
quiescent, or intermediate according to their dust-corrected rest-frame colors
and magnitudes. We employ a "delayed-then-rapid" quenching model that relates a
simulated cluster mass accretion rate to the observed numbers of each type of
galaxy in the cluster to constrain . For galaxies of mass , we find a quenching timescale of 1.24 Gyr
in the cluster sample, and 1.50 Gyr at . Using values
drawn from the literature, we compare the redshift evolution of to
timescales predicted for different physical quenching mechanisms. We find
to depend on host halo mass such that quenching occurs over faster timescales
in clusters relative to groups, suggesting that properties of the host halo are
responsible for quenching high-mass galaxies. Between and , we
find that evolves faster than the molecular gas depletion timescale and
slower than an SFR-outflow timescale, but is consistent with the evolution of
the dynamical time. This suggests that environmental quenching in these
galaxies is driven by the motion of satellites relative to the cluster
environment, although due to uncertainties in the atomic gas budget at high
redshift, we cannot rule out quenching due to simple gas depletion
The Gibbs-Thomson formula at small island sizes - corrections for high vapour densities
In this paper we report simulation studies of equilibrium features, namely
circular islands on model surfaces, using Monte-Carlo methods. In particular,
we are interested in studying the relationship between the density of vapour
around a curved island and its curvature-the Gibbs-Thomson formula. Numerical
simulations of a lattice gas model, performed for various sizes of islands,
don't fit very well to the Gibbs-Thomson formula. We show how corrections to
this form arise at high vapour densities, wherein a knowledge of the exact
equation of state (as opposed to the ideal gas approximation) is necessary to
predict this relationship. Exploiting a mapping of the lattice gas to the Ising
model one can compute the corrections to the Gibbs-Thomson formula using high
field series expansions. We also investigate finite size effects on the
stability of the islands both theoretically and through simulations. Finally
the simulations are used to study the microscopic origins of the Gibbs-Thomson
formula. A heuristic argument is suggested in which it is partially attributed
to geometric constraints on the island edge.Comment: 27 pages including 7 figures, tarred, gzipped and uuencoded. Prepared
using revtex and espf.sty. To appear in Phys. Rev.
Mesh inlay, mesh kit or native tissue repair for women having repeat anterior or posterior prolapse surgery: randomised controlled trial (PROSPECT)
Funding The project was funded by the National Institute for Health Research Health Technology Assessment Programme (Project Number 07/60/18). The Health Services Research Unit and the Health Economics Research Unit are funded by the Chief Scientist Office of the Scottish Government Health and Social Care Directorates. Acknowledgements The authors wish to thank the women who participated in the PROSPECT study. We also thank Margaret MacNeil for her secretarial support and data management; Dawn McRae and Lynda Constable for their trial management support; the programming team in CHaRT, led by Gladys McPherson; members of the Project Management Group for their ongoing advice and support of the study; and the staff at the recruitment sites who facilitated the recruitment, treatment and follow up of study participants.Peer reviewedPublisher PD
ALMA Observations of Gas-Rich Galaxies in z~1.6 Galaxy Clusters: Evidence for Higher Gas Fractions in High-Density Environments
We present ALMA CO (2-1) detections in 11 gas-rich cluster galaxies at z~1.6,
constituting the largest sample of molecular gas measurements in z>1.5 clusters
to date. The observations span three galaxy clusters, derived from the Spitzer
Adaptation of the Red-sequence Cluster Survey. We augment the >5sigma
detections of the CO (2-1) fluxes with multi-band photometry, yielding stellar
masses and infrared-derived star formation rates, to place some of the first
constraints on molecular gas properties in z~1.6 cluster environments. We
measure sizable gas reservoirs of 0.5-2x10^11 solar masses in these objects,
with high gas fractions and long depletion timescales, averaging 62% and 1.4
Gyr, respectively. We compare our cluster galaxies to the scaling relations of
the coeval field, in the context of how gas fractions and depletion timescales
vary with respect to the star-forming main sequence. We find that our cluster
galaxies lie systematically off the field scaling relations at z=1.6 toward
enhanced gas fractions, at a level of ~4sigma, but have consistent depletion
timescales. Exploiting CO detections in lower-redshift clusters from the
literature, we investigate the evolution of the gas fraction in cluster
galaxies, finding it to mimic the strong rise with redshift in the field. We
emphasize the utility of detecting abundant gas-rich galaxies in high-redshift
clusters, deeming them as crucial laboratories for future statistical studies.Comment: 8 pages, 3 figures, published in ApJ Letters; updated to match
published versio
Possible composite-fermion liquid as a crossover from Wigner crystal to bubble phase in higher Landau level
The ground state cohesive energies per electron of the composite fermion (CF)
Fermi sea, the Laughlin state and the charge density wave (CDW) at higher
Landau levels (LLs) are computed. It is shown that whereas for LL,
the CDW state is generally more energetically preferable than those of the CF
liquid and the Laughlin liquid, the CF liquid state unexpectedly
has lower ground state energy than that of the CDW state. We suggest this CF
liquid between the Wigner crystal and the bubble phase may lead to the
crossover from the normal integer quantum Hall liquid to the novel re-entrant
integer quantum Hall state observed in the recent magneto-transport
experiments
A Feasibility Study of Quantifying Longitudinal Brain Changes in Herpes Simplex Virus (HSV) Encephalitis Using Magnetic Resonance Imaging (MRI) and Stereology.
OBJECTIVES: To assess whether it is feasible to quantify acute change in temporal lobe volume and total oedema volumes in herpes simplex virus (HSV) encephalitis as a preliminary to a trial of corticosteroid therapy. METHODS: The study analysed serially acquired magnetic resonance images (MRI), of patients with acute HSV encephalitis who had neuroimaging repeated within four weeks of the first scan. We performed volumetric measurements of the left and right temporal lobes and of cerebral oedema visible on T2 weighted Fluid Attenuated Inversion Recovery (FLAIR) images using stereology in conjunction with point counting. RESULTS: Temporal lobe volumes increased on average by 1.6% (standard deviation (SD 11%) in five patients who had not received corticosteroid therapy and decreased in two patients who had received corticosteroids by 8.5%. FLAIR hyperintensity volumes increased by 9% in patients not receiving treatment with corticosteroids and decreased by 29% in the two patients that had received corticosteroids. CONCLUSIONS: This study has shown it is feasible to quantify acute change in temporal lobe and total oedema volumes in HSV encephalitis and suggests a potential resolution of swelling in response to corticosteroid therapy. These techniques could be used as part of a randomized control trial to investigate the efficacy of corticosteroids for treating HSV encephalitis in conjunction with assessing clinical outcomes and could be of potential value in helping to predict the clinical outcomes of patients with HSV encephalitis
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