Effects of the light regime on nutrient assimilation by phytoplankton in the Baja California and northwest Africa upwelling systems

The ability of natural phytoplankton assemblages in the Baja California and northwest Africa upwelling regions to assimilate nitrate, ammonium and silicic acid at simulated in situ light intensity and in the dark, was determined in 6 hr tracer experiments using the stable isotopes 15N and 30Si. Nitrate was almost never taken up in the dark, but dark uptake rates of ammonium and silicic acid ranged from zero to values equal to those measured at saturating light intensity...

Distribution, fluxes and biological utilization of inorganic nitrogen during a spring bloom in the New York Bight

The utilization of ammonium (NH4) and nitrate (NO3) by natural phytoplankton communities was measured during a Ceratium tripos-dominated spring bloom in the New York Bight…

Comparison of electrically driven lasers for space power transmission

High-power lasers in space could provide power for a variety of future missions such as spacecraft electric power requirements and laser propulsion. This study investigates four electrically pumped laser systems, all scaled to 1-MW laser output, that could provide power to spacecraft. The four laser systems are krypton fluoride, copper vapor, laser diode array, and carbon dioxide. Each system was powered by a large solar photovoltaic array which, in turn, provided power for the appropriate laser power conditioning subsystem. Each system was block-diagrammed, and the power and efficiency were found for each subsystem block component. The copper vapor system had the lowest system efficiency (6 percent). The CO2 laser was found to be the most readily scalable but has the disadvantage of long laser wavelength

A 50-kW Module Power Station of Directly Solar-Pumped Iodine Laser

The conceptual design of a 50 kW Directly Solar-Pumped Iodine Laser (DSPIL) module was developed for a space-based power station which transmits its coherent-beam power to users such as the moon, Martian rovers, or other satellites with large (greater than 25 kW) electric power requirements. Integration of multiple modules would provide an amount of power that exceeds the power of a single module by combining and directing the coherent beams to the user's receiver. The model developed for the DSPIL system conservatively predicts the laser output power (50 kW) that appears much less than the laser output (93 kW) obtained from the gain volume ratio extrapolation of experimental data. The difference in laser outputs may be attributed to reflector configurations adopted in both design and experiment. Even though the photon absorption by multiple reflections in experimental cavity setup was more efficient, the maximum secondary absorption amounts to be only 24.7 percent of the primary. However, the gain volume ratio shows 86 percent more power output than theoretical estimation that is roughly 60 percent more than the contribution by the secondary absorption. Such a difference indicates that the theoretical model adopted in the study underestimates the overall performance of the DSPIL. This fact may tolerate more flexible and radical selection of design parameters than used in this design study. The design achieves an overall specific power of approximately 5 W/kg and total mass of 10 metric tons

The effect of sewage on uptake of inorganic nitrogen and carbon by natural populations of marine phytoplankton

The short-term effect of sewage effluent on nitrogen and carbon productivity of natural marine phytoplankton obtained near two California outfalls has been studied. Uptake of ammonium was shown to be inhibited at much lower effluent concentrations than was carbon uptake. Since the populations studied were shown to exhibit Michaelis-Menten kinetics for ammonium uptake, a precise measurement of inhibition could be obtained. The results have immediate application since the phytoplankton populations of the area studied have been shown previously to be nitrogen limited

Site-specific perturbations of alpha-synuclein fibril structure by the Parkinson's disease associated mutations A53T and E46K.

PMCID: PMC3591419This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Parkinson's disease (PD) is pathologically characterized by the presence of Lewy bodies (LBs) in dopaminergic neurons of the substantia nigra. These intracellular inclusions are largely composed of misfolded α-synuclein (AS), a neuronal protein that is abundant in the vertebrate brain. Point mutations in AS are associated with rare, early-onset forms of PD, although aggregation of the wild-type (WT) protein is observed in the more common sporadic forms of the disease. Here, we employed multidimensional solid-state NMR experiments to assess A53T and E46K mutant fibrils, in comparison to our recent description of WT AS fibrils. We made de novo chemical shift assignments for the mutants, and used these chemical shifts to empirically determine secondary structures. We observe significant perturbations in secondary structure throughout the fibril core for the E46K fibril, while the A53T fibril exhibits more localized perturbations near the mutation site. Overall, these results demonstrate that the secondary structure of A53T has some small differences from the WT and the secondary structure of E46K has significant differences, which may alter the overall structural arrangement of the fibrils

A Self-Reference False Memory Effect in the DRM Paradigm: Evidence from Eastern and Western Samples

It is well established that processing information in relation to oneself (i.e., selfreferencing) leads to better memory for that information than processing that same information in relation to others (i.e., other-referencing). However, it is unknown whether self-referencing also leads to more false memories than other-referencing. In the current two experiments with European and East Asian samples, we presented participants the Deese-Roediger/McDermott (DRM) lists together with their own name or other people’s name (i.e., “Trump” in Experiment 1 and “Li Ming” in Experiment 2). We found consistent results across the two experiments; that is, in the self-reference condition, participants had higher true and false memory rates compared to those in the other-reference condition. Moreover, we found that selfreferencing did not exhibit superior mnemonic advantage in terms of net accuracy compared to other-referencing and neutral conditions. These findings are discussed in terms of theoretical frameworks such as spreading activation theories and the fuzzytrace theory. We propose that our results reflect the adaptive nature of memory in the sense that cognitive processes that increase mnemonic efficiency may also increase susceptibility to associative false memories

Overview of field operations during a 2013 research expedition to the southern Beaufort Sea on the RV Araon

Research experiments conducted and preliminary findings The Expedition ARA04C is a multidisciplinary research program in the Beaufort Sea, carried out in collaboration between the Korea Polar Research Institute (KOPRI), Geological Survey of Canada (GSC), Department of Fisheries and Ocean (DFO), Monterey Bay Aquarium Research Institute (MBARI), and the Alfred Wegener Institute (AWI). The Expedition ARA04C on the IBRV Araon took place from September 6 to September 24, 2013 (Figure 0.1). Multiple research experiments were undertaken to study geological processes related to degrading permafrost, fluid flow and degassing, and associated geohazards, paleo-oceanography of the Beaufort shelf and slope region, as well as physical and chemical oceanography measurement of the Arctic Ocean linked with continuous atmospheric studies. The expedition focused on two main research areas: offshore Barrow, Alaska, from September 7 to September 9, 2013, and the Canadian Beaufort Sea from September 10 to September 24, 2013. Multichannel seismic data, in conjunction with an ocean-bottom-seismometer (OBS) study were collected to support drilling proposals especially IODP pre-proposal #806 (Dallimore et al., 2012), and to verify distribution and internal structures of the offshore permafrost occurrences (Figure 0.2). The multi-channel seismic data were acquired on the outer continental shelf of the Canadian Beaufort Sea, totaling 14 lines with ~435 line-kilometers and ~4,500 shot gathers (Chapter 3). The combined multichannel seismic and OBS data will be processed post-expedition at KOPRI and the GSC, and will allow detailed velocity analyses to investigate the permafrost signature and help mapping zones of high-velocity sediments indicative of the presence of ice (Chapter 4). Individual shot gathers collected during the multichannel seismic program show clear refraction arrivals with velocities around 2000m/s in areas of expected permafrost occurrence, and shot gathers lacked such arrivals in zones where the permafrost was predicted to be absent. It is therefore expected that the OBS data, once processed, will also show clear refracted arrivals for velocity analyses. Continuous sub-bottom profiler (SBP) and multibeam data were collected along all ship tracks for detailed subsurface imaging of sediment structures and permafrost, as well as for core-site location verification (Chapter 5 and 6). During Expedition ARA04C, more than 3000 line-kilometers of SBP data were collected, co-located with multibeam and backscatter data. These data are an essential part of the study of sub-seafloor permafrost distribution and provide insights into sediment dynamics at critical boundaries, such as the shelf edge. Along the shelf edge, the occurrence of pingo-like features (PLFs) result in a rugged landscape with thousands of PLFs piercing through the otherwise laminated sediments. More than 30 crossings of this critical shelf-edge boundary were made during this expedition, which complement data acquired in 2012 with the Huntec system and 3.5 kHz data provided by ArcticNet as part of the regional multibeam map of the study area. High resolution data provided critical new insights in deep-water fluid expulsion zones. Key new data were acquired over the area of the "Gary Knolls", where PLF structures occur at the shelf edge in water depth of only 50 to 60 m. All SBP data from this expedition will be post-processed and analyzed for the presence of sub-seafloor permafrost, occurrence of the PLF structures and indications for fluid and gas migration. Multibeam and backscatter data were collected along all ship tracks, adding to the database of existing information gathered through previous expeditions to the study region. Heat flow measurements were undertaken at eight stations (Figure 0.3) to study the thermal structure of fluid expulsion features, as well as degrading permafrost along a slope-shelf transect in the eastern Mackenzie Trough (Chapter 7). The data provide critical constraints on the distribution of sub-seafloor permafrost as well as the gas hydrate stability zone around fluid expulsion features. A very important finding is the observation made at the mud volcano in 420 m water depth, where seafloor temperatures are the highest in all observed stations, indicating active mud volcanism. Geological sampling using gravity coring and multi-coring tools was performed at strategic sites to support two research objectives. The first objective was to provide key data towards ongoing international research linked to IODP pre-proposals #753 (O'Regan et al., 2010) and #806 (Dallimore et al., 2012). The second objective was to collect core to define key seismo-stratigraphic horizons critical to the understanding of geohazards in the region (Chapter 8). In total, 21 gravity cores and 12 multi-cores were taken (Figure 0.4, Table 8.3). All cores were scanned with a multi-sensor core-logger to measure physical properties (Chapter 9). Most sediment analyses on the cores will be performed post-expedition at KOPRI, GSC, and laboratories of other University-based collaborators in Canada and Germany. Onboard, sub-samples were taken from all shallow multi-cores and selected gravity cores. On selected cores from the Canadian Beaufort study region pore-waters were extracted using rhizones. These samples will be analyzed postexpedition at MBARI. Water sampling and Conductivity-Temperature-Depth (CTD) profiling was undertaken at most core sites to study physical and chemical properties of the seawater (Figure 0.5). These station-measurements were complemented by continuous waterproperty and atmospheric measurements when the Araon was underway. Most samples taken will be analyzed post-expedition at KOPRI for DIC/TA, nutrients, DOC, and POC. The pH of seawater, underway data of pCO2, CH4, and N2O, as well as a variety of subsequent calculations is required for accurate estimates in the above listed parameters. Methane was also measured with a methane sensor attached to the CTD tool and at the mud volcano in 420 m water depth, methane concentrations of more than 100-times ocean background were seen. The methane plume was also acoustically imaged with the echo sounder systems on board the IBRV Araon. Further details on the water sampling and atmospheric measurements are given in Chapter 10 and 11