Detection of Histone Modifications in Plant Leaves

Chromatin structure is important for the regulation of gene expression in eukaryotes. In this process, chromatin remodeling, DNA methylation, and covalent modifications on the amino-terminal tails of histones H3 and H4 play essential roles1-2. H3 and H4 histone modifications include methylation of lysine and arginine, acetylation of lysine, and phosphorylation of serine residues1-2. These modifications are associated either with gene activation, repression, or a primed state of gene that supports more rapid and robust activation of expression after perception of appropriate signals (microbe-associated molecular patterns, light, hormones, etc.)3-7

Time-dependent density functional approach for the calculation of inelastic x-ray scattering spectra of molecules

7 páginas, 5 figuras.-- et al.We apply time-dependent density functional theory to study the valence electron excitations of molecules and generalize the typically used time-propagation scheme and Casida’s method to calculate the full wavevector dependent response function. This allows the computational study of dipole-forbidden valence electron transitions and the dispersion of spectral weight as a function of the wavevector. The method provides a novel analysis tool for spectroscopic methods such as inelastic x-ray scattering and electron energy loss spectroscopy. We present results for benzene and CF3Cl and make a comparison with experimental results.Acknowledge HPCEuropa2 program, Academy of Finland (1127462, Centers of Excellence Program 2006–2011, and National Graduate School in Materials Physics) for financial support, and CSC-IT Center for Science Ltd for computational resources. A.R. acknowledges Spanish MEC (FIS2007-65702-C02-01), ACI-Promociona (ACI2009-1036), Grupos Consolidados UPV/EHU del Gobierno Vasco (IT-319-07), the European Union through e-13 ETSF (Contract No. 211956), and THEMA (Contract No. 228539) projects, as well as the support by the Red Española de Supercomputación and ARINA.Peer reviewe

Monitoreo descriptivo de parásitos de origen fecal hallados en hortalizas de hojas

p.271-276El objetivo de este trabajo fue contabilizar la presencia de protozoos, huevos y larvas de helmintos en muestras de hortalizas de hoja para consumo fresco, que se comercializan en el Mercado Central de Buenos Aires. Las especies analizadas fueron: lechuga, radicheta, berro y perejil. Las muestras se procesaron por los métodos de Baerman y Flotación. Los resultados obtenidos indican que: 1- Existe contaminación parasitaria: sobre un total de 98 muestras analizadas durante 12 meses, el 27,5por ciento estaban contaminadas. 2- La especie más contaminada fue berro (66,7por ciento), luego radicheta (31,8por ciento), lechuga (21,4por ciento) y, finalmente, perejil (13,6por ciento). 3- El parásito más frecuente fue Entamoeba coli (44,4por ciento) y en segundo término E.hystolitica (22,4por ciento). 4- El sur y sudoeste del conurbano bonaerense fue la zona de mayor contaminación (66,6por ciento). La presencia de los contaminantes en las hortalizas estudiadas demuestra el desconocimiento de los riesgos potenciales que ciertas prácticas agrícolas pueden acarrear a los consumidores

Интеграция малого инновационного бизнеса в систему особых экономических зон

В рамках деятельности по стимулированию и развитию малого бизнеса на протяжении уже довольно длительного времени проводились разного рода меры. Однако на сегодняшний день доля малого и среднего бизнеса не превышает 21% ВВП. В структуре малого бизнеса преобладают предприятия, занятые в торговой сфере, а доля инновационных предприятий ничтожна мала. Проблему развития малых инновационных предприятий и повышения их экономической эффективности, необходимо решать путем интеграции данных предприятий с институтом особой экономической зоны технико-внедренческого типа

Оценка экономической эффективности инновационного проекта в IT-сфере

Цель работы - разработка комплекса мероприятий по совершенствованию проектной деятельности в IT-сфере (на примере IT-проекта "UMKA"). В процессе исследования проводились изучения теоретических основ проектной деятельности компании, особенности реализации инновационных проектов в IT-сфере, анализировались модели, методы и показатели оценки эффективности IT-проекта, выявлялись особенности оценки эффективности проектов в IT-сфере. В практической части проводилась оценка эффективности IT-проекта "UMKA", разработанная и реализуемая ООО "I-link".The work purpose - development of complex of measures on improvement of project activities in the IT sphere (on the example of an IT project "UMKA"). In the process of research was conducted to study the theoretical basics of project activities of the company, peculiarities of realization of innovative projects in the IT field, we have analyzed the models, methods and indicators to measure the effectiveness of an IT project and identified the features of estimation of efficiency of projects in the IT sphere. In the practical part of the evaluation of the effectiveness of IT-project "UMKA", developed and implemented by "I-link"

A bifunctional dermaseptin–thanatin dipeptide functionalizes the crop surface for sustainable pest management

To reduce pesticide use while preserving crop productivity, alternative pest and disease control measures are needed. We thought of an alternative way of functionalizing leaves of soybean to fight its most severe disease, Asian soybean rust (Phakopsora pachyrhizi). To do so, we produced bifunctional peptides that adhere to the soybean leaf surface and prevent the germination of P. pachyrhizi spores. In detail, amphiphilic peptides liquid chromatography peak I (LCI), thanatin (THA), tachystatin A2 (TA2), and lactoferricin B (LFB) were all fused to enhanced green fluorescent protein (eGFP). Of these fusion peptides, eGFP–LCI and eGFP–THA bound strongly and in a rainfast manner to the surface of soybean, barley, and corn leaves. eGFP–THA binding to soybean also withstood high temperature, sunlight and biotic degradation for at least 17 days. The dipeptides seem to bind mainly to the surface wax layer of leaves because eGFP–THA and eGFP–LCI did not stick to the wax-depleted cer-j59 mutant of barley or to corn leaves with their surface wax removed. A fusion of the antimicrobial peptide dermaseptin 01 and THA (DS01–THA) inhibits the germination of P. pachyrhizi spores in vitro and reduces Asian soybean rust disease in a rainfast manner. Therefore, this study reveals that bifunctional peptides can be used to functionalize the crop surface for sustainable disease management

Kesiapan Petugas Puskesmas dalam Penanggulangan Infeksi Menular Seksual dan Hiv/aids pada Pelayanan Antenatal

Background: Prevalence HIV/AIDS among housewife has been increasing which was notice by higher cases of HIVpositive babies. An effective intervention effort in the health sector is to accelerate the decreasing of Maternal Mortality Rate (MMR) through integrated approach of reproductive health care and STD HIV/AIDS. One of reproductive health services was to prevention and treatment of STD-HIV/AIDS. When this program was integrated with antenatal care it will prevents transmission of HIV from mother to child. The program called Prevention Mother To Child Transmission or PMTCT. Recently in Indonesia program antenatal care has not yet optimally integrated with STD-HIV/AIDS service. The objectiive of this study was to know how far preparedness of health center personnel in prevention transmission of STD, HIV/AIDS integrated with antenatal care. Methods: This study was exploration research. Respondents of this study were personals of public health center that have task related to antenal care and prevention program of STD, HIV/AIDS. Locations of study were Surabaya Malang Blitar Tulungagung, Semarang, Surakarta, Denpasar and Batam. Dara collection was done by interviewed and Focus Group Discussion. Results: The result of the study revealed the unprepared-ness of PHC personnel in term of knowledge about reproductive health and skill in universal precaution practice in integrated program of prevention STD, HIV/AIDS and antenatal care. Lack of facilities and its supporting system including the laboratory equipment and reagents also make activities of this integrated program being obstacle. Health service program still conducted fragmented because there was no standard operational procedure (SOP) of integrated program of STD, HIV/AIDS prevention and antenatal care. It was suggested to conduct a comprehensive program of reproductive health by increasing health personnel knowledge and skill with training; arrange SOP of integrated program of STD, HIV/AIDS prevention and antenatal care. It also needs to complete the facility with laboratory equipment i.e. rapid test and drugs to support this integrated program

Profiling carbohydrate composition, biohydrogen capacity, and disease resistance in potato

Background: Potato ( Solanum tuberosum ) is one of the most important sources of carbohydrates in human diet. Because of its high carbohydrate levels it recently has also received attention in biohydrogen production. To exploit the natural variation of potato with respect to resistance to major diseases, carbohydrate levels and composition, and capacity for biohydrogen production we analyzed tubers of native, improved, and genetically modified potatoes, and two other tuberous species for their glucose, fructose, sucrose, and starch content. Results: High-starch potato varieties were evaluated for their potential for Caldicellulosiruptor saccharolyticus -mediated biohydrogen production with Desir\ue9e and Rosita varieties delivering the highest biohydrogen amounts. Native line Vega1 and improved line Yagana were both immune to two isolates (A291, A287) of Phytophthora infestans . Conclusions: Our data demonstrate that native potato varieties might have great potential for further improving the multifaceted use of potato in worldwide food and biohydrogen production