Gatekeeping in Master’s Level Rehabilitation Counseling Programs: An Exploratory Study

The current study explores attitudes and procedures of gatekeeping in rehabilitation counseling programs. Using the Theory of Planned Behavior (TPB), the current study explores underlying causes for a lack of gatekeeping discussion and action in rehabilitation counseling. Rehabilitation educators and program directors from both CORE and CACREP-accredited rehabilitation counseling programs participated in the online Gatekeeping Attitudes and Procedures Survey (GAPS). Results indicate that, while attitudes towards gatekeeping were positive, programs lack formalized procedures. TPB aids in understanding that attitudes towards gatekeeping may not influence educator behavior on the matter. Implications include further research into this area to better understand the relationship between gatekeeping and rehabilitation counselor education, as well as the impact of gatekeeping on student and faculty experiences

Computer-based cognitive intervention for aphasia: Behavioural and neurobiological outcomes

Aphasia, an acquired impairment of language that commonly occurs after stroke, can have significant consequences on all aspects of functioning of affected individuals. Some have proposed that the language deficits observed in aphasia are due to underlying limitations in cognitive processes that support language1-3. This ‘cognitive’ theory of aphasia is gaining increased attention in the research literature4, and is the impetus for the study of treatments for aphasia that target these underlying cognitive processes5-8. Indeed, studies of cognitive interventions in healthy populations have reported positive outcomes in behavioural (i.e. language and overall cognitive functioning9, 10) as well as neurobiological (i.e., brain function and/or structure11-13) domains, offering promise for the application of these types of interventions to aphasia. Recently, computer-based ‘brain training’ programs have become increasingly prevalent. BrainFitness (BF) is one such commercially available program; it has been used to show improvement in auditory processing speed, attention and working memory in typically aging adults14, 15. This program has the potential to be a useful intervention for individuals with aphasia, but questions regarding the clinical utility of the program and neural correlates of training-related behavioural changes remain. The purpose of this study was to investigate the effect of BF training in people with aphasia using behavioural and neurobiological outcome measures

Brief report: A pilot study of the use of a virtual reality headset in autism populations

The application of virtual reality technologies (VRTs) for users with autism spectrum disorder (ASD) has been studied for decades. However, a gap remains in our understanding surrounding VRT head-mounted displays (HMDs). As newly designed HMDs have become commercially available (in this study the Oculus Rift™) the need to investigate newer devices is immediate. This study explored willingness, acceptance, sense of presence and immersion of ASD participants. Results revealed that all 29 participants (mean age=32; 33% with IQ< 70) were willing to wear the HMD. The majority of the participants reported an enjoyable experience, high levels of ‘presence’, and were likely to use HMDs again. IQ was found to be independent of the willingness to use HMDs and related VRT immersion experience

Gene expression signatures affected by alcohol-induced DNA methylomic deregulation in human embryonic stem cells

AbstractStem cells, especially human embryonic stem cells (hESCs), are useful models to study molecular mechanisms of human disorders that originate during gestation. Alcohol (ethanol, EtOH) consumption during pregnancy causes a variety of prenatal and postnatal disorders collectively referred to as fetal alcohol spectrum disorders (FASDs). To better understand the molecular events leading to FASDs, we performed a genome-wide analysis of EtOH's effects on the maintenance and differentiation of hESCs in culture. Gene Co-expression Network Analysis showed significant alterations in gene profiles of EtOH-treated differentiated or undifferentiated hESCs, particularly those associated with molecular pathways for metabolic processes, oxidative stress, and neuronal properties of stem cells. A genome-wide DNA methylome analysis revealed widespread EtOH-induced alterations with significant hypermethylation of many regions of chromosomes. Undifferentiated hESCs were more vulnerable to EtOH's effect than their differentiated counterparts, with methylation on the promoter regions of chromosomes 2, 16 and 18 in undifferentiated hESCs most affected by EtOH exposure. Combined transcriptomic and DNA methylomic analysis produced a list of differentiation-related genes dysregulated by EtOH-induced DNA methylation changes, which likely play a role in EtOH-induced decreases in hESC pluripotency. DNA sequence motif analysis of genes epigenetically altered by EtOH identified major motifs representing potential binding sites for transcription factors. These findings should help in deciphering the precise mechanisms of alcohol-induced teratogenesis

Listeria monocytogenes Exploits Host Caveolin for Cell-to-Cell Spreading

Listeria monocytogenes moves from one cell to another using actin-rich membrane protrusions that propel the bacterium toward neighboring cells. Despite cholesterol being required for this transfer process, the precise host internalization mechanism remains elusive. Here, we show that caveolin endocytosis is key to this event as bacterial cell-to-cell transfer is severely impaired when cells are depleted of caveolin-1. Only a subset of additional caveolar components (cavin-2 and EHD2) are present at sites of bacterial transfer, and although clathrin and the clathrin-associated proteins Eps15 and AP2 are absent from the bacterial invaginations, efficient L. monocytogenes spreading requires the clathrin-interacting protein epsin-1. We also directly demonstrated that isolated L. monocytogenes membrane protrusions can trigger the recruitment of caveolar proteins in a neighboring cell. The engulfment of these bacterial and cytoskeletal structures through a caveolin-based mechanism demonstrates that the classical nanometer-scale theoretical size limit for this internalization pathway is exceeded by these bacterial pathogens

Plasmid origin of replication of herpesvirus papio: DNA sequence and enhancer function.

Herpesvirus papio (HVP) is a lymphotropic virus of baboons which is related to Epstein-Barr virus (EBV) and produces latent infection. The nucleotide sequence of the 5,775-base-pair (bp) EcoRI K fragment of HVP, which has previously been shown to confer the ability to replicate autonomously, has been determined. Within this DNA fragment is a region which bears structural and sequence similarity to the ori-P region of EBV. The HVP ori-P region has a 10- by 26-bp tandem array which is related to the 20- by 30-bp tandem array from the EBV ori-P region. In HVP there is an intervening region of 764 bp followed by five partial copies of the 26-bp monomer. Both the EBV and HVP 3' regions have the potential to form dyad structures which, however, differ in arrangement. We also demonstrate that a transcriptional enhancer which requires transactivation by a virus-encoded factor is present in the HVP ori-P

Oxidative stress in children late after Kawasaki disease: relationship with carotid atherosclerosis and stiffness

Background: Persistent arterial dysfunction in patients with a history of Kawasaki disease (KD) and an integral role of oxidative stress in the development of cardiovascular disease are increasingly recognized. We sought to test the hypothesis that oxidative stress is increased in KD patients and related to carotid atherosclerotic changes and stiffness. Methods: We compared the serum levels of oxidative stress biomarkers, carotid intima-media thickness (IMT), and carotid stiffness index among KD patients with coronary aneurysms (n = 32), those without coronary complications (n = 19), and controls (n = 32). Results: Compared with controls, patients with coronary aneurysms had significantly higher serum levels of malonaldehyde (2.62 ± 0.12 μM vs 2.22 ± 0.07 μM, p = 0.014) and hydroperoxides (26.50 ± 1.13 μM vs 22.50 ± 0.62 μM, p = 0.008). A linear trend of the magnitude of oxidative stress in relation to inflammatory damage was observed for malonaldehyde (p = 0.018) and hydroperoxides (p = 0.014) levels. Serum malonaldehyde and hydroperoxide levels correlated positively with carotid IMT (p < 0.001 and p = 0.034, respectively) and stiffness index (p = 0.001 and p = 0.021, respectively). Multiple linear regression analysis identified serum malonaldehyde level as a significant determinant of carotid IMT (β = 0.31, p = 0.006) and stiffness (β = 0.27, p = 0.008). Conclusion: Our findings suggestoxidative stress is increased in KD patients with coronary aneurysms and is associated with carotid intima-media thickening and stiffening. © 2008 Cheung et al; licensee BioMed Central Ltd.published_or_final_versio

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

Pasantía en el centro de rehabilitación Clinic of Rehabilitation of Wildlife (CROW), en Florida

Modalidad: PasantíaLa pasantía se realizó durante el periodo comprendido entre el 25 de marzo y el 21 de mayo del 2015 en el centro de rehabilitación en Sanibel Island, Florida. En el transcurso de las ocho semanas ingresaron 851 pacientes a la clínica, con la siguiente distribución: 71% aves, 25% mamíferos, 4% reptiles y 0.47% anfibios. Esta pasantía permitió realizar un triage de emergencia a los pacientes ingresando la clínica, la restricción física y química (según requerida), el examen físico general del animal y la elaboración del abordaje médico o quirúrgico inicial. También se aplicó el uso de exámenes colaterales como la hematología, radiología y exámenes coprológicos para llegar a un diagnóstico más certero. Entre los problemas más comunes que se presentaron en la clínica durante la pasantía se encontraron la intoxicación por marea roja, fracturas ocasionadas por atropello y heridas en la piel por ataques de depredador o por objetos como anzuelos y líneas de pesca, por lo que en el trabajo se describe el manejo médico y quirúrgico de estos problemas. Además, se discute acerca un un caso de un búho cornudo (Bubo virginianus) con fractura transversa del tibiotarso. También se presentan los protocolos para la crianza de mamíferos y aves juveniles, ya que un 14% de los animales eran huérfanos y un 14% de las aves ingresaron a Clinic of Rehabilitation of Wildlife (CROW) por caída del nido, y se describe tanto la nutrición como el ambiente que se debe proveer a los pacientes.From March 25 to May 21, 2015, an externship was carried out in the Clinic of Rehabilitation of Wildlife (CROW), located in Sanibel Island, Florida. During this period, 851 patients were admitted to the clinic with the following distribution: 71% were avian species, 25% mammals, 4% reptiles, and 0.47% amphibians. During the externship I participated actively in emergency triage of wildlife animals, performing a general physical examination on each patient, implementing physical and chemical restraint according to the species, and carrying out the initial treatment. The use of collateral exams such as bloodwork, radiographs, and fecal exams was also applied to obtain a more accurate diagnosis of the patient. Brevetoxicosis, fractures due to car accidents, skin wounds caused by predator attacks, and hook and line injuries were amongst the most common problems encountered in patients admitted at CROW during the externship. The present thesis describes the medical and surgical treatment of these problems, it also presents a clinical case of a Great Horned Owl (Bubo virginianus) with a transverse tibiotarsus fracture. General guidelines for the development and care of neonatal mammals and birds are also presented in this thesis, since 14% of the admitted patients were orphaned and 14% were birds that fell from their nest. Finally, feeding and housing guidelines of patients were also included.Escuela de Medicina Veterinari