Estudo de Caso - Síndrome Cardio-Fácio-Cutâneo

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Actividade anti-tumoral de extractos de Cynara cardunculus na via molecular de PI3K/AKT/mTOR

O presente trabalho visou avaliar o potencial anti-proliferativo de extractos de folhas (FCF) e caules (CCF) de Cynara cardunculus (Cc). Adicionalmente, pretendeu-se caracterizar molecularmente a actividade de extractos de FCF e CCF na via de sinalização do PI3K/AKT/mTOR, em células HepG2 e de cancro da mama de fenótipo triplo negativo MDA-MB-231. Os resultados mostraram que o extracto de FCF apresentou uma pronunciada actividade anti-proliferativa, com um valor de IC50 de 1,74 e 0,74 μg/mL, respectivamente para as células HepG2, e MDA-MB-231. Na presença do extracto FCF os níveis de expressão das isoformas (α, β, γ) do PI3K, nas células MDA-MB-231não apresentaram diferenças. Porém, a análise do nível de expressão proteica de AKT revelou uma diminuição significativa de expressão em células MDA-MB-231, quando tratadas com extracto de FCF. Estes resultados sublinham o potencial papel do extracto de FCF na regulação via do PI3K/AKT/mTOR, importante alvo terapêutico para o tratamento oncológico; ABSTRACT: The purpose of this study was to characterize the molecular activity of Cynara cardunculus extracts on the PI3K/AKT/mTOR signaling pathway, on HepG2 and MDA-MB-231 tumor cell lines. The results show that fresh cultivated leaf (FCL) extract has a pronounced anti-proliferative activity, with an IC50 of 1,74 μg/mL expressed in terms of total phenols presented on HepG2 and 0,74 μg/mL expressed in terms of total phenols presented on MDA-MB-231. On MDA-MB-321 cell line there is no significant difference among the expression level of p110 isoforms ( , , ) of the PI3K in presence of FCL extract. On the other hand, the protein expression level of total AKT seems to be sensitive to the presence of FCL extracts, since its protein level significantly decreases on MDA-MB-231 cells incubated with FCL extract. These results underlie the putative role of the FCL Cc extract on the regulation of the PI3K/AKT/mTOR pathway

Química forense no ensino básico

As Ciências Forenses constituem excelentes ferramentas para a elaboração de materiais podendo integrar diversos níveis e áreas de ensino pois são interdisciplinares e apelativas para os alunos fruto da elevada popularidade das séries televisivas. Nestes episódios são abordadas técnicas e procedimentos utilizados em Ciência representando um recurso para aplicação e desenvolvimento em sala de aula. Este permite uma abordagem Ciência-Tecnologia- Sociedade, (CTS), desenvolvendo metodologias orientadas para a Aprendizagem Baseada na Resolução de Problemas (ABRP) onde o professor abandona o papel tradicional tornando-se o fomentador da construção de conhecimento nos seus alunos. Nesta dissertação sugerem-se algumas actividades desenvolvidas no âmbito das Ciências Forenses que aplicam técnicas e conteúdos relacionados com a disciplina de Ciências Físico-Químicas do Ensino Básico. Estas actividades realizam-se em contexto formal, permitem que os alunos relacionem os conteúdos abordados na disciplina com a vida real e aproximam-nos da Ciência aumentando o gosto pela sua aprendizagem. A aplicação destas actividades teve um impacto positivo nos alunos que é demonstrado na análise do questionário realizado permitindo-lhes aumentar a aprendizagem da disciplina de Ciências Físico-Químicas, compreender a sua aplicabilidade, relevância no quotidiano e incrementar a literacia científica junto da população estudantil; Abstract: Use of Forensic Sciences in Middle School The forensic sciences are excellent tools for the preparation of materials to be integrated at various levels and areas of teaching because they are interdisciplinary and, moreover, very appealing to students which came as a result of the high popularity that the television series present. The episodes of these series show several techniques and procedures that are used in science that may represent a valuable resource to be applied and developed in the classroom. This feature allows a Science-Technology-Society (STS), and develop methodologies based on Problem Based Learning (PBL). In this methodology the teacher abandons the traditional role and passes to a secondary role in fostering the construction of knowledge in their students. In this thesis we suggest some activities within the forensic sciences field to be applied in the discipline of Physics and Chemistry of Basic Education. These activities take place in a formal context and allow students to relate the content covered in the discipline with real life and increment students‟ interest to learn science in general and chemistry in particular. The application of this type of methodology had a positive impact on students as demonstrated in the analysis of the questionnaire conducted in which students responded that this approach allowed them to enhance learning of the discipline of Physics and Chemistry, understand their applicability and relevance to daily life as well as contributes to increasing the scientific literacy among the student population

O balanced scorecard na gestão estratégica de uma empresa

O Balanced Scorecard (BSC) tem sido uma das matérias da área de gestão mais debatidas nas últimas décadas. As suas origens remontam a 1990, ano em que Robert Kaplan e David Norton iniciaram um estudo que envolvia doze empresas com o objectivo de desenvolverem um novo modelo do seu desempenho. O objectivo do trabalho é desenvolver um modelo de gestão e monitorização da estratégia de uma multinacional ligada ao sector automóvel, construindo para tal o Balanced Scorecard. Procurou-se identificar a missão, visão e estratégias usadas pela empresa, identificar quais as perspectivas a adoptar pela empresa, dando relevo àquela que deveria ser o foco principal e as demais, cumprindo o princípio de causa e efeito. Definiram-se as áreas chave de performance e os factores críticos de sucesso permitindo identificar os indicadores de gestão relevantes. O trabalho enquadra-se numa perspectiva qualitativa, sendo que o resultado final é um Tableaux de Bord constituído por quatro perspectivas integradas onde se encontram associados os indicadores, referidos pela empresa como essenciais, para a gestão da mesma. Conclui-se que a aplicação do Balanced Scorecard é adequada para a gestão de uma empresa do sector automóvel. //ABSTRACT: The Balanced Scorecard (BSC) has been one of the subjects in the area of management most debated in recent decades. Its origins go back to 1990, the year in which Robert Kaplan and David Norton began a study involving twelve companies with the aim of developing a new model of its performance. The aim of the work is to develop a model for managing and monitoring the strategy of a multinational linked to the automobile sector, building for that the Balanced Scorecard. The study seeks to identify the mission, vision and strategies used by the company, identify the prospects to be taken by the company, giving relief what should be the main focus and the others, complying with the principle of cause and effect. Have set up the key areas of performance and on the critical success factors enabling identify the relevant indicators of management. The work relate to a qualitative perspective, and the end result is a Tableaux de Bord constituted by four perspectives integrated where it is attached indicators, listed by the company as essential to the management of the same. It is concluded that the implementation of the Balanced Scorecard is suitable tor the management of a company in the automobile sector

Dentistry Students’ Knowledge of Pediatric Patients Who Suffered Violence: A Systematic Review

Objective: To review the literature unprecedentedly to identify the dentistry students\u27 knowledge of pediatric patients who suffered violence. Material and Methods: A systematic review was conducted using PubMed, Web of Science, LILACS, SciELO, Google Scholar, and OpenGrey electronic databases up to November 2021. The "Grey Literature" was verified through Google Scholar and Open Grey searches to avoid any selection bias. There was no restriction on the date of publication or language. Results: The systematic search yielded 2.756 studies in the first selection phase, but only twenty-two articles were included. All selected articles were published between 1998 and 2021 and used a questionnaire to evaluate the dentistry students\u27 knowledge regarding child maltreatment cases. Brazil was the country that had the most studies included (10 articles). Despite the majority of the students presenting insufficient knowledge about child maltreatment, evidence from this research showed that every form of approach by professionals toward child maltreatment should be considered important. Conclusion: Therefore, there are deficiencies regarding the teaching-learning methodology, reinforcing the need for improvements in Dentistry undergraduate curricula

INDICAÇÃO E CONTRAINDICAÇÃO DA FISIOTERAPIA NAS FASES II E III NO PÓS-OPERATÓRIO DE CIRURGIAS CARDÍACAS: Revisão de literatura

INTRODUÇÃO: As principais metas dos programas de reabilitação cardiovascular (PRC) são: permitir aos cardiopatas retornar à vida produtiva e ativa, dentro das limitações impostas pelo seu processo patológico (LIMA, 2011) Segundo Bellini (1997), A reabilitação cardiovascular (RCV) pode ser conceituada como um ramo de atuação da cardiologia que implementada por equipe de trabalho multiprofissional, permite a restituição, ao indivíduo, de uma satisfatória condição clínica, física, psicológica e laborativa. DESENVOLVIMENTO: A reabilitação cardiovascular estar tradicionalmente dividida em diversas fases, sequenciada como FASE I, FASE II e FASES III e IV (MARQUES, 2004). OBJETIVOS: Analisar os critérios da indicação e contraindicação da fisioterapia nas fases II e III no pós-operatório de cirurgias cardíacas. METODOLOGIA: O presente estudo trata-se de uma revisão bibliográfica sobre as indicações e contraindicações da fisioterapia nas fases II e III no pós-operatório de cirurgias cárdicas, foram utilizados como recurso de pesquisa os sites eletrônicos de revista científica como: Scielo, Lilacs, Medline e google acadêmico, selecionando os idiomas português e inglês. RESULTADOS E DISCUSSÕES: Foram encontrados 50 artigos, após leitura dos artigos, 23 foram excluídos, restando 27 que compuseram a leitura para o estudo. A reabilitação cardíaca foi definida pela primeira vez no ano de (2000) por Giannini; Forti; Diament, sendo a arte e ciência de restituir ao indivíduo o grau de atividade física e mental, compatível com as capacidades funcionais do coração. CONCLUSÃO: pode se observar que a reabilitação cardíaca tem se atualizado e cada vez mais é comprovada a importância de sua indicação aos pacientes.

African Histoplasmosis

African histoplasmosis (AH) is a fungal infection due to Histoplasma capsulatum var. duboisii. Unlike classic histoplasmosis, infections caused by H. duboisii most commonly affects the skin, subcutaneous tissues, lymph nodes and bones. Till present, in Europe, all published cases have been from natives from Africa who moved to Europe. There are few reports in the literature describing the radiologic features of AH.The authors report a case of a 7-year-old girl, from Guine-Bissau, who had multiple skin and subcutaneous lesions and left lower limb deformity. The presence of H. duboisii was confirmed by culture and histologic examination. Imaging studies were performed and showed multiple calcified and non-calcified subcutaneous lesions compatible with granulomas/abscesses; axillary and thoracic lymphadenopathies; and signs of chronic osteomyelitis. Although these imaging features are not pathognomic they should raise awareness for the diagnosis of AH in patients that are African or people who have travelled from this continent. Imaging plays a crucial role not only in the diagnosis of this disease but also in monitoring the response to therapy and detecting recurrence

Nursing students’ knowledge regarding sexuality, sex, and gender diversity in a multicenter study

Introduction: Sexuality is an integral part of development and personality, and is important in healthcare. Nurses are among the most representative healthcare professionals. For holistic and inclusive nursing care practice and to improve equality, human rights, well-being, and health of individuals, the curricula of nursing courses must integrate broad knowledge about sexuality and its diversity. This study aimed to identify and analyze nursing students’ knowledge of sexuality, sex, and gender diversity. The present study was part of a multicenter study conducted in Europe. Methods: Questionnaires were administered in three nursing schools to assess nursing students’ knowledge (n = 75). Data processing was performed using Excel® software version 20 and IRaMuTeQ (R Interface pour les Analysis Multidimensionnelles de Textes et de Questionnaires) 0.7 alpha 2, allowing organization by category and subsequent thematic analysis using content analysis. Results: The textual corpus “Nursing students’ knowledge about sexuality in its diversity,” was divided into two sub-corpus: “Students’ perception of sexuality” and “Students’ perception of gender identity,” originating Class 6 “Eroticism” (14.23%) and Classes 4 “Sexual Orientation” (16.07%) and 3 “Heteronormative” (16.07%), the latter with greater proximity to each other and consequently to Class 6. Similarly, Classes 1 “Gender” (20.36%) and 5 “Cisgender” (12.14%) also presented a greater interrelationship between themselves and consecutively with Class 2 “Gender Identity” (15.36%). Discussion: The analyses revealed that though nursing students possessed knowledge about sexuality and its diversity, this knowledge was elementary and did not reveal a sustained appropriation of concepts related to sexuality, sexual orientation, and gender diversity. For some questions, the absence of students’ answers were noteworthy, and may be associated with their personal reservation in expressing themselves on this sensitive and intimate theme. To ensure diversity, inclusivity, and impartiality in nursing practice, it is imperative to change the curriculum plans of nursing courses to address the theme of sexuality during the training process of nurses in Europe.info:eu-repo/semantics/publishedVersio