101 research outputs found
Avaliação de desempenho de protótipo de secador solar passivo para tratamento de efluente de processo de dessalinização por destilação multi-efeito
O projecto AQUASOL-Enhanced Zero Discharge Seawater Desalination using Hybrid Solar Technology (EVK1-CT2001-00102) teve como principais objectivos a melhoria do desempenho económico e a minimização dos impactes ambientais associados a um processo de dessalinização por Destilação Multi-Efeito (MED)(Blanco et al, 2002). No âmbito do projecto, decorre o estudo de um secador solar passivo utilizado no tratamento do efluente produzido no processo de dessalinização, salmoura, através da sua concentração e/ou através da produção directa de sal a partir do efluente. Nesta etapa adicional do processo de dessalinização foi considerada não só a maior concentração do efluente relativamente à concentração da água marinha utilizada no processo MED, mas também a sua possível integração numa salina tradicional, com o potencial de substituir etapas intermédias da evaporação com vantagem face ao processo convencional de produção de sal marinho. Após um estudo das especificidades da produção de sal em Salinas tradicionais e do processo de evaporação de uma camada de salmoura, a construção e avaliação de cinco configurações distintas de um protótipo preliminar de secador solar passivo, permitiu o desenho final de um secador para a recuperação de sal a partir do efluente do processo MED (Collares Pereira et al, 2004), construido numa salina industrial na ilha de Lesvos (Grécia) (Collares Pereira et al, 2005). Neste artigo serão apresentados os primeiros resultados de operação do protótipo, bem como uma avaliação inicial do seu desempenho
Evidence of extensive mitochondrial introgression with nearly complete substitution of the typical Squalius pyrenaicus-like mtDNA of the Squalius alburnoides complex (Cyprinidae) in an independent Iberian drainage
Overcoming inertia : drivers of the outsourcing process
Almost all managers have directly or indirectly been involved in the practice of outsourcing in recent years. But as they know, outsourcing is not straightforward. Outsourcing inertia, when companies are slow to adapt to changing circumstances that accommodate higher outsourcing levels, may undermine a firm’s performance. This article investigates the presence of outsourcing inertia and the factors that help managers overcome it. Using statistical evidence, we show that positive performance effects related to outsourcing can accumulate when circumstances change. This is then followed by rapid increases in outsourcing levels (i.e. outsourcing processes). We investigate what gives rise to these outsourcing processes through follow-up interviews with sourcing executives, which suggest five drivers behind outsourcing processes: managerial initiative (using outside experience); hierarchy (foreign headquarters); imitation (of competitors and of similar firms); outsider advice (from external institutions); knowledge sources (using external information). These five drivers all offer scope for managerial action. We tie them to academic literatures and suggest ways of investigating their presence and impact on the outsourcing process. Overall, we conclude that while economizing factors play a key role in explaining how much firms outsource, it is socializing factors that tend to drive outsourcing processes
May a hybridogenetic complex regenerate the nuclear genome of both sexes of a missing ancestor? First evidence on the occurrence of a nuclear non‐hybrid Squalius alburnoides
Reproductive success of nuclear nonhybrid males of Squalius alburnoides hybridogenetic complex (Teleostei, Cyprinidae): An example of interplay between female choice and ecological pressures?
The hybridogenetic fish complex Squalius
alburnoides comprises diploid males with non-hybrid
nuclear genomes and several hybrid forms varying in
ploidy and relative proportions of the parental genomes. In
this paper, we present evidence that in captivity females
prefer to mate with non-hybrid males. We suggest that
female choice combined with different ecological requirements
of hybrid and non-hybrid males may explain the
extreme variation in the relative abundance of male types
among drainages
Borrelia valaisiana resist complement-mediated killing independently of the recruitment of immune regulators and inactivation of complement components
Spirochetes belonging to the Borrelia (B.) burgdorferi sensu lato complex differ in their resistance to complement-mediated killing, particularly in regard to human serum. In the present study, we elucidate the serum and complement susceptibility of B. valaisiana, a genospecies with the potential to cause Lyme disease in Europe as well as in Asia. Among the investigated isolates, growth of ZWU3 Ny3 was not affected while growth of VS116 and Bv9 was strongly inhibited in the presence of 50% human serum. Analyzing complement activation, complement components C3, C4 and C6 were deposited on the surface of isolates VS116 and Bv9, and similarly the membrane attack complex was formed on their surface. In contrast, no surface-deposited components and no aberrations in cell morphology were detected for serum-resistant ZWU3 Ny3. While further investigating the protective role of bound complement regulators in mediating complement resistance, we discovered that none of the B. valaisiana isolates analyzed bound complement regulators Factor H, Factor H-like protein 1, C4b binding protein or C1 esterase inhibitor. In addition, B. valaisiana also lacked intrinsic proteolytic activity to degrade complement components C3, C3b, C4, C4b, and C5. Taken together, these findings suggest that certain B. valaisiana isolates differ in their capability to resist complement-mediating killing by human serum. The molecular mechanism utilized by B. valaisiana to inhibit bacteriolysis appears not to involve binding of the key host complement regulators of the alternative, classical, and lectin pathways as already known for serum-resistant Lyme disease or relapsing fever borreliae
Fine-Scale Phylogeographic Structure of Borrelia lusitaniae Revealed by Multilocus Sequence Typing
Borrelia lusitaniae is an Old World species of the Lyme borreliosis (LB) group of tick-borne spirochetes and prevails mainly in countries around the Mediterranean Basin. Lizards of the family Lacertidae have been identified as reservoir hosts of B. lusitaniae. These reptiles are highly structured geographically, indicating limited migration. In order to examine whether host geographic structure shapes the evolution and epidemiology of B. lusitaniae, we analyzed the phylogeographic population structure of this tick-borne bacterium using a recently developed multilocus sequence typing (MLST) scheme based on chromosomal housekeeping genes. A total of 2,099 questing nymphal and adult Ixodes ricinus ticks were collected in two climatically different regions of Portugal, being ∼130 km apart. All ticks were screened for spirochetes by direct PCR. Attempts to isolate strains yielded 16 cultures of B. lusitaniae in total. Uncontaminated cultures as well as infected ticks were included in this study. The results using MLST show that the regional B. lusitaniae populations constitute genetically distinct populations. In contrast, no clear phylogeographic signals were detected in sequences of the commonly used molecular markers ospA and ospC. The pronounced population structure of B. lusitaniae over a short geographic distance as captured by MLST of the housekeeping genes suggests that the migration rates of B. lusitaniae are rather low, most likely because the distribution of mediterranean lizard populations is highly parapatric. The study underlines the importance of vertebrate hosts in the geographic spread of tick-borne microparasites
Mate choice drives evolutionary stability in a hybrid complex
Previous studies have shown that assortative mating acts as a driver of speciation by countering
hybridization between two populations of the same species (pre-zygotic isolation) or
through mate choice among the hybrids (hybrid speciation). In both speciation types, assortative
mating promotes speciation over a transient hybridization stage. We studied mate
choice in a hybrid vertebrate complex, the allopolyploid fish Squalius alburnoides. This complex
is composed by several genomotypes connected by an intricate reproductive dynamics.
We developed a model that predicts the hybrid complex can persist when females
exhibit particular mate choice patterns. Our model is able to reproduce the diversity of population
dynamic outcomes found in nature, namely the dominance of the triploids and the
dominance of the tetraploids, depending on female mate choice patterns and frequency of
the parental species. Experimental mate choice trials showed that females exhibit the preferences
predicted by the model. Thus, despite the known role of assortative mating in driving
speciation, our findings suggest that certain mate choice patterns can instead hinder
speciation and support the persistence of hybrids over time without speciation or extinction
Seed storage conditions change the germination pattern of clonal growth plants in Mediterranean salt marshes.
8 páginas, 4 tablas, 8 figuras.The effect of salinity level and extended exposure to different salinity and flooding conditions on germination patterns of three saltmarsh clonal growth plants (Juncus subulatus, Scirpus litoralis, and S. maritimus) was studied. Seed exposure to extended flooding and saline conditions significantly affected the outcome of the germination process in a different, though predictable, way for each species, after favorable conditions for germination were restored. Tolerance of the germination process was related to the average salinity level measured during the growth/germination season at sites where established individuals of each species dominated the species cover. No relationship was found between salinity tolerance of the germination process and seed response to extended exposure to flooding and salinity conditions. The salinity response was significantly related to the conditions prevailing in the habitats of the respective species during the unfavorable (nongrowth/nongermination) season. Our results indicate that changes in salinity and hydrology while seeds are dormant affect the outcome of the seed-bank response, even when conditions at germination are identical.
Because these environmental-history-dependent responses differentially affect seed germination, seedling density, and probably sexual recruitment in the studied and related species, these influences should be considered for wetland restoration and managementFinancial support from the Spanish Ministry of the Environment (MMA, project 05/99) and the Junta de Andalucía (research group 4086)enabled us to carry out the present work.Peer reviewe
A polymorphic microsatellite from the Squalius alburnoides complex (Osteichthyes, Cyprinidae) cloned by serendipity can be useful in genetic analysis of polyploids
A new microsatellite locus (SAS1) for Squalius alburnoides was obtained through cloning by serendipity. The possible usefulness of this new species-specific microsatellite in genetic studies of this hybrid-species complex, was explored. The polymorphism exhibited by SAS1 microsatellite is an important addition to the set of microsatellites previously used in genetic studies in S. alburnoides complex, that mostly relied in markers described for other species. Moreover, the SAS1 microsatellite could be used to identify the parental genomes of the complex, complementing other methods recently described for the same purpose.
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