An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations

Many herpetofauna species have been introduced outside of their native range. MtDNA barcoding is regularly used to determine the provenance of such populations. The alpine newt has been introduced across the Netherlands, the United Kingdom and Ireland. However, geographical mtDNA structure across the natural range of the alpine newt is still incompletely understood and certain regions are severely undersampled. We collect mtDNA sequence data of over seven hundred individuals, from both the native and the introduced range. The main new insights from our extended mtDNA phylogeography are that 1) haplotypes from Spain do not form a reciprocally monophyletic clade, but are nested inside the mtDNA clade that covers western and eastern Europe; and 2) haplotypes from the northwest Balkans form a monophyletic clade together with those from the Southern Carpathians and Apuseni Mountains. We also home in on the regions where the distinct mtDNA clades meet in nature. We show that four out of the seven distinct mtDNA clades that comprise the alpine newt are implicated in the introductions in the Netherlands, United Kingdom and Ireland. In several introduced localities, two distinct mtDNA clades co-occur. As these mtDNA clades presumably represent cryptic species, we urge that the extent of genetic admixture between them is assessed from genome-wide nuclear DNA markers. We mobilized a large number of citizen scientists in this project to support the collection of DNA samples by skin swabbing and underscore the effectiveness of this sampling technique for mtDNA barcoding

The conservation status of the world’s reptiles

MB and MR were funded by a grant from the Esmee Fairbairn Foundation, BC by the Rufford Foundation. North American and Mexican species assessments were funded by the Regina Bauer Frankenberg Foundation for Animal Welfare. Species assessments under the Global Reptile Assessment (GRA) initiative are supported by: Moore Family Foundation, Gordon and Betty Moore Foundation, Conservation International, Critical Ecosystem Partnership Fund (CEPF), and European Commission. Additional acknowledgements are included in the online supplementary material. The assessment workshop for Mexican reptiles was kindly hosted by Ricardo Ayala and the station personnel of the Estacion de Biologia Chamela, Institut de Biologia, Universidad Nacional Autonoma de Mexico. Workshop and logistical organisation of the Philippines assessments was provided by the Conservation International Philippines Office, in particular Ruth Grace Rose Ambal, Melizar V. Duya and Oliver Coroza. Workshop and logistical organisation for the European Reptile and Amphibian Assessments was provided by Doga Dernegi, in particular Ozge Balkiz and Ozgur Koc. Workshop and logistical organisation for assessments of sea snakes and homalopsids was provided by the International Sea Turtle Symposium and Dr. Colin Limpus (Australian Government Environmental Protection Agency). Special thanks to Jenny Chapman (EPA) and Chloe Schaub le (ISTS). Thank you also to Dr. Gordon Guymer (Chief Botanist Director of Herbarium) for accommodating us at the Herbarium in the Brisbane Botanical Gardens, and Mark Read and Kirsten Dobbs (Great Barrier Reef Marine Parks Association) and Dave Pollard and Brad Warren (Ocean Watch Australia) for institutional support. Mohamed Bin Zayed Species Conservation Fund, Conservation International Madagascar and the Darwin Initiative contributed to funding the costs of the Madagascar reptile workshop.Effective and targeted conservation action requires detailed information about species, their distribution, systematics and ecology as well as the distribution of threat processes which affect them. Knowledge of reptilian diversity remains surprisingly disparate, and innovative means of gaining rapid insight into the status of reptiles are needed in order to highlight urgent conservation cases and inform environmental policy with appropriate biodiversity information in a timely manner. We present the first ever global analysis of extinction risk in reptiles, based on a random representative sample of 1500 species (16% of all currently known species). To our knowledge, our results provide the first analysis of the global conservation status and distribution patterns of reptiles and the threats affecting them, highlighting conservation priorities and knowledge gaps which need to be addressed urgently to ensure the continued survival of the world’s reptiles. Nearly one in five reptilian species are threatened with extinction, with another one in five species classed as Data Deficient. The proportion of threatened reptile species is highest in freshwater environments, tropical regions and on oceanic islands, while data deficiency was highest in tropical areas, such as Central Africa and Southeast Asia, and among fossorial reptiles. Our results emphasise the need for research attention to be focussed on tropical areas which are experiencing the most dramatic rates of habitat loss, on fossorial reptiles for which there is a chronic lack of data, and on certain taxa such as snakes for which extinction risk may currently be underestimated due to lack of population information. Conservation actions specifically need to mitigate the effects of human-induced habitat loss and harvesting, which are the predominant threats to reptiles.Esmee Fairbairn FoundationRufford FoundationRegina Bauer Frankenberg Foundation for Animal WelfareMoore Family FoundationGordon and Betty Moore FoundationConservation International, Critical Ecosystem Partnership Fund (CEPF)European Commission Joint Research CentreZayed Species Conservation FundConservation International MadagascarDarwin Initiativ

Variation in life history traits in Bombina bombina from the lower Danube floodplain

International audienc

Population age structure and growth in four syntopic amphibian species inhabiting a large river floodplain

International audienceRiver floodplains are disturbance-dominated landscapes where floods are major regulators of both aquatic and nearby terrestrial communities. Amphibians are common inhabitants of floodplains and their life cycle depends on both aquatic and terrestrial habitats. We focused on how different syntopic species of amphibians reacted to the environmental conditions of a large river floodplain. We examined life-history traits such as population age structure and growth in small- and large-bodied species of anurans and urodeles in the lower Danube River floodplain in Romania. Two newt species, Triturus vulgaris (small-bodied) and Triturus dobrogicus (large-bodied), and two anuran taxa, Bombina bombina (small-bodied) and the Rana esculenta complex (large-bodied), were included in the study. The ages of individuals estimated by skeletochronology varied from 3 to 5-6 years for T. vulgaris and from 2-3 to 4-5 years for T. dobrogicus. In the anurans, ages varied from 2 to 5 years in B. bombina and from 4 to 10 years in the R. esculenta complex. The numbers of breeding opportunities (i.e., the number of years the adults reproduce) are similar in both newt species (3), while growth rates and age at maturity differ between the large- and small-bodied species. In anurans, the number of breeding opportunities for the smallest species, B. bombina (4), is associated with a high growth rate and earlier maturation. In the larger R. esculenta complex, the higher number of breeding opportunities (7) is associated with a low growth rate and delayed maturation. The study of age distribution and associated parameters provides useful information on population life history. We discuss how age structure and growth of amphibian populations in large river floodplains can be used as indicators of environmental conditions

Older males whistle better: age and body size are encoded in the mating calls of a nest-building amphibian (Anura: Leptodactylidae)

Courtship acoustic displays in anuran amphibians are energetically costly and risky, but have a major role in mating success since they encode relevant information regarding the caller’s identity and status. Age and size are essential traits shaping fitness, reproductive success and life-history strategies, and thus are expected to also have a role in courtship displays. We tested this assumption in a species of nest-building frogs, Leptodactylus bufonius, in northern Argentina. We conducted the first detailed quantitative description of the males’ mating calls and assessed the effects of biological traits (i.e., body size parameters and individual age) and local climate (i.e., air temperature and humidity) on the main acoustic features of these calls (i.e., call duration, inter-call duration, dominant frequency, and dominant frequency modulation). The calls were short (mean ± SE, 0.163 ± 0.004 s), whistle-like, single notes with harmonic structure. The dominant frequency (1381.7 ± 16.2 Hz) decreased with arm length (χ2 = 5.244, df = 1, p = 0.022) and had an upward modulation (456.4 ± 11.0 Hz) which increased with age (χ2 = 4.7012, df = 1, p = 0.030). Call duration and dominant frequency were the most static parameters at intra-individual level, indicating their role in individual recognition. Temperature and humidity shaped the temporal acoustic parameters, and the dominant frequency. Our findings suggest that the acoustic features of the mating calls in amphibians could promote female mate choice in relation to both size and age and open up new questions for future research: are females more attracted to older males, and what are the specific costs and benefits? We suggest that mating calls may direct female preferences toward males of certain size and age classes, ultimately shaping the life-history strategies in a given population. Finally, we found discrepancies in the mating calls of L. bufonius recorded from Corrientes and those previously described from other populations, which suggests that multiple species may have been recorded under the same name.Fil: Stanescu, F.. Ovidius University; RumaniaFil: Marquez, R.. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales; EspañaFil: Cogalniceanu, D.. Ovidius University; RumaniaFil: Marangoni, Federico. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas, Naturales y Agrimensura. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentin

The declining Spadefoot toad, Pelobates fuscus (Pelobatidae): paleo and recent environmental changes as a major influence on current population structure and status

International audiencePopulations of the European Spadefoot toad (Pelobates fuscus) have experienced recent declines all over Europe, but these appear to be more intense in north and western Europe. Due to the toad's fossorial nature and specific habitat requirements, environmental conditions have played a major role in structuring current populations. We examined the phylogeographic structure in P. fuscus from 16 localities throughout Europe using mitochondrial cytochrome b gene sequence analysis. Sequence divergence among haplotypes was low (0.54 +/- 0.15%). Three very closely related haplotypes occupy northern and western parts of Europe whereas 12 others were observed among samples from south-eastern Europe, including the Balkans. Our results suggest that toads only recently colonized the northern and western parts of Europe following glacial retreat. This expansion probably took place in steppic-like areas during the younger Dryas cold interval, about 12,900-11,500 years ago. Restricted gene flow with an isolation-by-distance population structure characterises a major part of its distribution range. Based on our results we suggest that the northern and western lineages should be considered as distinct conservation units, while the south-eastern populations from the refugial areas, where nearly all genetic polymorphism occurs and populations appear less vulnerable, should receive special attention