Putting Writers at Rest into Motion

Many students whose studies emphasize science-based fields often struggle to understand why writing skills are important and necessary. For this reason, writing center tutors have the opportunity to adjust tutoring practices in such a way that students are equipped for both educational and professional experiences. Research has shown that the majority of college graduates lack basic written communication skills employers look for, suggesting a disconnect between English classes and other writing situations. As tutors understand the struggles of science-based students, they can adjust tutoring practices such that students are comfortable learning about writing and translate those skills into all aspects of written communication. Tutoring in this manner avoids the pitfall of writing education being tailored too closely to specific situations and becoming inflexible. As students learn to write for various situations, they additionally improve their reasoning abilities, the skills they often find to be the most valuable

A Communication Analysis of Religion in Education

The researchers chose to examine religion as a factor in the decision to attend a university and the way that students adapt communicatively to faith-based universities. The concept brought many aspects of communication into play, but only a specific group of theories were applicable to the research findings. The researchers selected two theories to help explain the data: Communication Accommodation Theory and Symbolic Interactionism Theory. Both, Communication Accommodation Theory and Symbolic Interactionism Theory, focus on the decision-making and adaptation aspects of the study

Hip Strength and Core Endurance in Female Adolescent Runners With and Without Knee Pain

BACKGROUND AND PURPOSE: Patellofemoral pain syndrome (PFPS) is one of the most prevalent orthopedic conditions affecting young athletes today. Epidemiological studies have reported PFPS to be the most common injury seen in runners. Deficits in hip strength have been identified in runners with PFPS, but core endurance in relation to knee pain has not been well documented. The primary purpose of our study was to investigate differences in hip strength and core endurance between female, adolescent runners with PFPS and their age matched controls. The secondary purpose of our research was to examine any correlations between hip strength and core endurance in our participants. METHODS: A cross sectional design was used. We recorded pain, Kujala score, hip strength and endurance and core endurance in 34 adolescent female cross country runners. Cases with PFPS were defined as young female runners with a minimum three month history of anterior knee pain of insidious onset and had a most severe knee pain rated 3/10 or higher. Control subjects had no history of knee surgery, traumatic knee injuries, patellar instability, or neurologic conditions. Between-group differences and correlations were calculated between age-matched cases and controls using t-tests. Pearson correlation coefficients were used to determine associations for selected measures. RESULTS: No significant differences were observed between cases and controls for hip strength and endurance. However, there was a large percent difference between cases and controls in selected core endurance measures. It was found that all hip strength and core endurance results had low correlations ( \u3c 0.28). Among cases with PFPS, a strong and significant, negative correlation was found between subjects’ reported worst pain and Kujala score (r=-0.79, p\u3c0.05)). A non-significant moderate negative correlation between side plank endurance and usual pain was found (r=-0.49). CONCLUSION: There were minimal differences noted in isometric strength tests between groups. There was a clear difference noted with endurance testing between groups. However, this difference was not found to be significant, which could be due to low number of subjects with PFPS. The differences in endurance between athletes with PFPS and their pain free counterparts merit further investigation and research. Of note, it was found that strength and endurance had a minimal correlation; this indicates that clinically, endurance cannot be inferred from isometric strength testing. Therefore, we recommend clinicians perform specific measures of endurance when attempting to identify impairments in runners with PFPS

A single H/ACA small nucleolar RNA mediates tumor suppression downstream of oncogenic RAS.

Small nucleolar RNAs (snoRNAs) are a diverse group of non-coding RNAs that direct chemical modifications at specific residues on other RNA molecules, primarily on ribosomal RNA (rRNA). SnoRNAs are altered in several cancers; however, their role in cell homeostasis as well as in cellular transformation remains poorly explored. Here, we show that specific subsets of snoRNAs are differentially regulated during the earliest cellular response to oncogenic RASG12V expression. We describe a novel function for one H/ACA snoRNA, SNORA24, which guides two pseudouridine modifications within the small ribosomal subunit, in RAS-induced senescence in vivo. We find that in mouse models, loss of Snora24 cooperates with RASG12V to promote the development of liver cancer that closely resembles human steatohepatitic hepatocellular carcinoma (HCC). From a clinical perspective, we further show that human HCCs with low SNORA24 expression display increased lipid content and are associated with poor patient survival. We next asked whether ribosomes lacking SNORA24-guided pseudouridine modifications on 18S rRNA have alterations in their biophysical properties. Single-molecule Fluorescence Resonance Energy Transfer (FRET) analyses revealed that these ribosomes exhibit perturbations in aminoacyl-transfer RNA (aa-tRNA) selection and altered pre-translocation ribosome complex dynamics. Furthermore, we find that HCC cells lacking SNORA24-guided pseudouridine modifications have increased translational miscoding and stop codon readthrough frequencies. These findings highlight a role for specific snoRNAs in safeguarding against oncogenic insult and demonstrate a functional link between H/ACA snoRNAs regulated by RAS and the biophysical properties of ribosomes in cancer

Influence of Exercise Type on Maternal Blood Pressure Adaptation throughout Pregnancy

BACKGROUND: It has been reported that 10% of all pregnancies are complicated by a hypertensive disorder of pregnancy. Previous research has shown that moderate-vigorous intensity exercise has a positive effect on maternal resting blood pressure. A research gap, however, exists related to how different types of exercise (resistance, aerobic, combined resistance and aerobic) affect maternal blood pressure. Most of the previous studies solely focused on aerobic exercise. OBJECTIVE: The aim of this study was to examine the effects of exercise types on maternal blood pressure throughout pregnancy. STUDY DESIGN: This study employed a secondary analysis using data from a randomized controlled prenatal exercise intervention trial. This study utilized 3 exercise intervention groups (aerobic, resistance, combination) and compared the results with those of a nonexercize control group. Participants completed 3 50-minute sessions weekly from 16 weeks of gestation until delivery. Maternal vital signs and physical measurements such as systolic blood pressure, diastolic blood pressure, and heart rate were measured every 4 weeks throughout the intervention period. Between-group mean differences in maternal measurements were assessed using Pearson's chi-square tests for continuous (age, prepregnancy body mass index, heart rate, systolic blood pressure, diastolic blood pressure, pulse pressure) variables. For gravida, exact Wilcox 2-sample tests were performed to determine between-group differences in mean values. Hierarchical linear growth curves were used to estimate maternal trajectories of systolic blood pressure and diastolic blood pressure from 16 weeks to 36 weeks’ gestation in each of the 4 groups (aerobic, combination, control, and resistance). RESULTS: There were no differences among the groups in maternal age or prepregnancy body mass index. Controlling for maternal body mass index, the lowest significant systolic blood pressure curve was noted throughout the pregnancy for women who participated in resistance exercise, followed by women in the aerobic exercise group all relative to the no exercise control group. At 36 weeks’ gestation, the systolic blood pressure was lower in the resistance group by 12.17 mm Hg (P<.001) and in the aerobic group by 7.90 mm Hg (P<.001) relative to controls. No significant change in systolic blood pressure was noted in the combination group in comparison with controls at 36 weeks’ gestation. Similarly, we demonstrated a significantly lower linear growth curve in diastolic blood pressure that was maintained throughout pregnancy in any exercise type relative to controls. After controlling for maternal body mass index, all 3 exercise types (combination, resistance, and aerobic) significantly predicted a similar decrease in diastolic blood pressure that was maintained throughout pregnancy. At 36 weeks’ gestation, the diastolic blood pressure was lower in the aerobic group by 7.30 mm Hg (P<.01), in the combination group by 6.43 mm Hg (P<.05), and in the resistance group relative to controls. CONCLUSION: Overall, all exercise types were beneficial in lowering maternal resting blood pressure throughout pregnancy. Resistance training was noted to be the most beneficial in improving systolic blood pressure, followed by aerobic exercise. All 3 exercise groups were noted to improve diastolic blood pressure equally. Further research needs to be done to determine if either resistance or aerobic exercise throughout pregnancy decreases the risk for hypertensive disorders of pregnancy and the associated morbidity and mortality

Kinetics of H2–O2–H2O redox equilibria and formation of metastable H2O2 under low temperature hydrothermal conditions

Author Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Geochimica et Cosmochimica Acta 75 (2011): 1594-1607, doi:10.1016/j.gca.2010.12.020.Hydrothermal experiments were conducted to evaluate the kinetics of H2(aq) oxidation in the homogeneous H2-O2-H2O system at conditions reflecting subsurface/near-seafloor hydrothermal environments (55-250 oC and 242-497 bar). The kinetics of the water-forming reaction that controls the fundamental equilibrium between dissolved H2(aq) and O2(aq), are expected to impose significant constraints on the redox gradients that develop when mixing occurs between oxygenated seawater and high- temperature anoxic vent fluid at near-seafloor conditions. Experimental data indicate that, indeed, the kinetics of H2(aq)-O2(aq) equilibrium become slower with decreasing temperature, allowing excess H2(aq) to remain in solution. Sluggish reaction rates of H2(aq) oxidation suggest that active microbial populations in near-seafloor and subsurface environments could potentially utilize both H2(aq) and O2(aq), even at temperatures lower than 40 oC due to H2(aq) persistence in the seawater/vent fluid mixtures. For these H2-O2 disequilibrium conditions, redox gradients along the seawater/hydrothermal fluid mixing interface are not sharp and microbially-mediated H2(aq) oxidation coupled with a lack of other electron acceptors (e.g. nitrate) could provide an important energy source available at low-temperature diffuse flow vent sites. More importantly, when H2(aq)-O2(aq) disequilibrium conditions apply, formation of metastable hydrogen peroxide is observed. The yield of H2O2(aq) synthesis appears to be enhanced under conditions of elevated H2(aq)/O2(aq) molar ratios that correspond to abundant H2(aq) concentrations. Formation of metastable H2O2 is expected to affect the distribution of dissolved organic carbon (DOC) owing to the existence of an additional strong oxidizing agent. Oxidation of magnetite and/or Fe++ by hydrogen peroxide could also induce formation of metastable hydroxyl radicals (•OH) through Fenton-type reactions, further broadening the implications of hydrogen peroxide in hydrothermal environments.This research was conducted with partial support from the NSF OCE-0752221 and the Geophysical Laboratory Postdoctoral Fellowship. We would also like to acknowledge contributions by the W.M. Keck Foundation and Shell towards supporting the hydrothermal lab at the Geophysical Lab. SMS acknowledges support from NSF OCE-0452333 and the Alfried-Krupp Wissenschaftskolleg Greifswald (Germany), while WES acknowledges support from NSF grants OCE-0549457 and OCE- 0813861

The NANOGrav 15-year Data Set: Observations and Timing of 68 Millisecond Pulsars

We present observations and timing analyses of 68 millisecond pulsars (MSPs) comprising the 15-year data set of the North American Nanohertz Observatory for Gravitational Waves (NANOGrav). NANOGrav is a pulsar timing array (PTA) experiment that is sensitive to low-frequency gravitational waves. This is NANOGrav's fifth public data release, including both "narrowband" and "wideband" time-of-arrival (TOA) measurements and corresponding pulsar timing models. We have added 21 MSPs and extended our timing baselines by three years, now spanning nearly 16 years for some of our sources. The data were collected using the Arecibo Observatory, the Green Bank Telescope, and the Very Large Array between frequencies of 327 MHz and 3 GHz, with most sources observed approximately monthly. A number of notable methodological and procedural changes were made compared to our previous data sets. These improve the overall quality of the TOA data set and are part of the transition to new pulsar timing and PTA analysis software packages. For the first time, our data products are accompanied by a full suite of software to reproduce data reduction, analysis, and results. Our timing models include a variety of newly detected astrometric and binary pulsar parameters, including several significant improvements to pulsar mass constraints. We find that the time series of 23 pulsars contain detectable levels of red noise, 10 of which are new measurements. In this data set, we find evidence for a stochastic gravitational-wave background.Comment: 90 pages, 74 figures, 6 tables; published in Astrophysical Journal Letters as part of Focus on NANOGrav's 15-year Data Set and the Gravitational Wave Background. For questions or comments, please email [email protected]

Design and synthesis of novel FtsZ-targeting antibacterial agents

Bacterial infections pose a major health concern worldwide, and the emergence of multi-drug resistant strains of bacteria has intensified the search for new antibiotic treatments with novel mechanisms of action. Bacterial cell division remains a new, currently untargeted pathway making it highly desirable research area for discovering new ways to kill bacteria. FtsZ is a highly conserved, essential bacterial cell division protein that forms the dynamic Z-ring involved in recruitment of other essential proteins and serving as the constricting force for cell division. Inhibiting the function of this key protein disrupts proper cell division, and has therefore become a key target in the search for new antibiotics. There are many known FtsZ inhibitors spanning from natural products such as sanguinarine and berberine, to synthetic derivatives such as GTP analogs and PC190723. The majority of these known inhibitors, however, lack the proper potency, safety profiles, and physiochemical properties required for clinical development. Initial studies involved modifying the structures of two known FtsZ inhibitors, sanguinarine and berberine, to increase potency and explore the structure-activity relationships. Unfortunately, although successfully obtaining analogs with good antibacterial activity, these compounds lacked desired solubility properties for further advancement. This led to the second generation of compounds that lacked a constitutive cationic charge while retaining the antibacterial activity seen with the previous analogs. While these compounds had much better solubility, they possessed a new drawback. Compounds from the second generation were found to be highly protein bound resulting in a significant loss of antibacterial activity when administered in the presence of protein. Studies began to design and synthesize analogs that either exhibited much higher potency or much less protein binding. Although initial attempts at this were unsuccessful, continued efforts are being made to find a FtsZ inhibitor with improved potency, physiochemical properties, and a broader spectrum of antibacterial activity.Ph. D.Includes bibliographical referencesby Cody Kelle