The efficiency of probiotics administrated via different routes and doses in enhancing production performance, meat quality, gut morphology, and microbial profile of broiler chickens

CITATION: Soumeh, E. A. et al. 2021. The efficiency of probiotics administrated via different routes and doses in enhancing production performance, meat quality, gut morphology, and microbial profile of broiler chickens. Animals, 11:3607, doi:10.3390/ani11123607.The original publication is available at https://www.mdpi.comTo study the efficiency of Bacillus spp. probiotics administered via different routes and doses, a 6-week grow-out trial was conducted using a total of 378 day-old mixed-sex ROSS308 broiler chickens in a completely randomized block design. Six experimental diets included probiotics added at two different inclusion rates into the feed (250 g/ton; PRO250, or 500 g/ton; PRO500), or in the drinking water (25 g/L; PRO-WS), or as a feed synbiotic (250 g probiotic + 250 g/ton prebiotic; SYN), compared to a negative (NC; without additives) and positive control (PC; with antibiotics) diets. The PRO-WS enhanced feed intake (p < 0.05) and tended to improve average daily gain and final body weight (p = 0.14). Broiler gut morphology in the duodenum including the villus height (p = 0.04), villus width (p = 0.05) and crypt depth (p = 0.02) were improved by PRO500. Firmicutes was the most abundant phylum, followed by Bacteroidetes. Streptococcaceae, Lachnoospiraceae, Peptostreptococcaceae, Ruminococcaceae, and Erysipe-lotrichaceae were the top five most abundant families. Antibiotic inclusion in PC reduced microbial beta-diversity and increased similarity compared to probiotic inclusion (p = 0.05). Probiotic inclusion reduced the relative abundance of Bacteroides fragilis, which is a commonly isolated pathogen and is considered as a marker for antimicrobial resistance. Overall, probiotic supplementation via feed or water may potentially improve the production performance of the broiler chickens, and water-soluble probiotics are potentially more effective. Probiotics, especially when added to water, suggest a promising feed additive to support gut microbial maturation and diversity, and may reduce resistant bacteria in broiler chickens. However, it is suggested that the best route for the administration of probiotics be further examined under commercial conditions to find the most effective and practical application method that yields the most consistent results.https://www.mdpi.com/2076-2615/11/12/3607Publisher's versio

Conversion of quinoa and lupin agro-residues into biochar in the Andes: An experimental study in a pilot-scale auger-type reactor

In the last decades, the cultivation of quinoa and lupin became an important source of income for Andean farmers due to the demand for high nutrient-density foods from the Global North. The increase in the cultivation intensity caused by this exogenous demand led to the overexploitation of local ecosystems and a decrease in soil fertility. As an alternative to recover and improve soil quality, this work uses a pilot-scale auger pyrolysis reactor, implemented in the Andes, to assess the conversion of the agro residues generated in the post-harvesting processes of quinoa and lupin into biochar for soil amendment. Following the European Biochar Certificate guidelines, the pyrolyzed quinoa stems can be classified as biochar while the pyrolyzed quinoa husks can be classified as pyrogenic carbonaceous material. Both can be used for soil amendment considering their molar ratios (H/Corg, O/Corg) and carbon content. It was not possible to carbonize lupin stems and seedcases. Despite the altitude (2,632 m.a.s.l), the CO concentration during the carbonization of quinoa stems and husks were 1,024.4 and 559 mg/Nm3, this last, near the European eco-design standard of 500 mg/Nm3. A subsequent SWOT analysis showed the need to explore low-cost and low-complexity pyrolysis reactors that allow the decentralized conversion of agro residues at the farm-scale. The development of local standards to regulate the production and use of biochar is also essential to grant the safety of the processes, the quality of the products, and mobilize funds that allow implementation at relevant scales

Ensayos de tratamientos de protección y consolidación de la policromía del alfarje y yeserías del Patio de las Doncellas: Real Alcázar de Sevilla

El trabajo que se presenta pone de manifiesto la metodología llevada a cabo para la valoración de tratamientos, en lo que a color se refiere, aplicados sobre probetas de ensayo realizadas a partir de los materiales identificados en dos elementos arquitectónicos decorativos de la zona sur-este de uno de los patios más significativos del Alcázar de Sevilla: el Patio de las Doncellas. Estos elementos que tenemos como motivo de estudio son el alfarje de las galerías bajas y el paramento de yeso. Los tratamientos seleccionados para ser evaluados consisten, en el caso del alfarje en la aplicación de un barniz de protección con estabilizador de radiación ultravioleta (Archival Golden ®), y en el caso de las yeserías de aplicación de dos tratamientos de consolidación: resina acrílica (Paraloid®B72) y silicato de etilo (Bioestel®1200). Los resultados obtenidos servirán de base para la elección de una propuesta de intervención óptima que incluya la posible utilización del barniz ultravioleta en el alfarje in situ así como el efecto que podrían tener los tratamientos de consolidación en las policromías de las yeserías. De esta forma contribuimos al conocimiento previo del comportamiento de los materiales antes de abordar la restauración de la decoración arquitectónica de este espacio o de otras obras de similar cronología con problemáticas similares.The work presented shows the methodology conducted for evaluating treatments, in what concerns color, applied on test specimens made from the materials identified in two decorative architectural elements of the south-east one of the most important courts of the Alcazar of Seville: courtyard of the Maidens. These elements we have as a subject of study are the paneled ceiling of the lower galleries and the wall plaster. The treatments selected for evaluation consist in the case of alfarje in applying a protective varnish with ultraviolet radiation stabilizer, and in the case of plasterworks, applying two consolidation treatments: Paraloid ®B72 and Ethyl Silicate (Bioestel®1200). The results will form the basis for choosing an optimal intervention proposal that includes the possible use of UV varnish on the paneled ceiling and the effect that could have treatments consolidation of polychrome plasterwork. In this way, we contribute to the prior knowledge of the behavior of materials before boarding the restoration of the architectural decoration of this space or other works of similar chronology with similar problems.Esta investigación ha sido financiada por el Ministerio de Economía y Competitividad y por el Fondo Europeo de Desarrollo Regional (FEDER) en el marco del Proyecto de Investigación “Estudio científico y tratamientos de conservación de revestimientos arquitectónicos de época romana a medieval” de referencia (HAR2015-66139-P) así como el proyecto del Ministerio de Innovación y Ciencia denominado “Decoración arquitectónica de tradición islámica. Materiales y técnicas de ejecución” (HAR 2011-27598). Igualmente ha sido posible gracias a la beca contrato puente de la Universidad de Granada de la que es beneficiaria Ana Isabel Calero Castillo

Synapse-associated protein 102/dlgh3 couples the NMDA receptor to specific plasticity pathways and learning strategies

Understanding the mechanisms whereby information encoded within patterns of action potentials is deciphered by neurons is central to cognitive psychology. The multiprotein complexes formed by NMDA receptors linked to synaptic membrane-associated guanylate kinase (MAGUK) proteins including synapse-associated protein 102 (SAP102) and other associated proteins are instrumental in these processes. Although humans with mutations in SAP102 show mental retardation, the physiological and biochemical mechanisms involved are unknown. Using SAP102 knock-out mice, we found specific impairments in synaptic plasticity induced by selective frequencies of stimulation that also required extracellular signal-regulated kinase signaling. This was paralleled by inflexibility and impairment in spatial learning. Improvement in spatial learning performance occurred with extra training despite continued use of a suboptimal search strategy, and, in a separate nonspatial task, the mutants again deployed a different strategy. Double-mutant analysis of postsynaptic density-95 and SAP102 mutants indicate overlapping and specific functions of the two MAGUKs. These in vivo data support the model that specific MAGUK proteins couple the NMDA receptor to distinct downstream signaling pathways. This provides a mechanism for discriminating patterns of synaptic activity that lead to long-lasting changes in synaptic strength as well as distinct aspects of cognition in the mammalian nervous system

Airbnb and crime in Barcelona (Spain): testing the relationship using a geographically weighted regression

The existence of works proving the possible relationship empirically that Airbnb lodgings could have with crime in Spain is not known. This research analyzes the relationship between Airbnb lodgings and crimes against the properties and people in Barcelona’s neighbourhoods. To achieve this, we use an ordinary least squares regression model and a geographically weighted regression model. The results show a significant and positive relationship between the higher density of Airbnb lodgings and the higher crime rates in the neighbourhoods, especially of patrimonial nature. Divided by type of leased space, the Airbnb homes, in which the guest shares a room with other guests, show a higher relationship with crimes against property and people. The results of the local model show a spatial heterogeneity in all variables used, indicating the need to address non-stationary spatial processes that reveal hidden patterns. However, the only variable that shows statistically significant local variability is the total Airbnb lodgings variable. Finally, we discussed some unexpected results, proposing some future lines of research. © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Nanjing Normal University

The ALMaQUEST Survey XIV: do radial molecular gas flows affect the star-forming ability of barred galaxies?

We investigate whether barred galaxies are statistically more likely to harbour radial molecular gas flows and what effect those flows have on their global properties. Using 46 galaxies from the ALMA-MaNGA QUEnching and STar formation (ALMaQUEST) survey, we identify galaxies hosting optical bars using a combination of the morphological classifications in Galaxy Zoo 2 and HyperLEDA. In order to detect radial molecular gas flows, we employ full 3D kinematic modelling of the ALMaQUEST 12CO(1-0) datacubes. By combining our bar classifications with our radial bar-driven flow detections, we find that galaxies classed as barred are statistically more likely to host large-scale radial gas motions compared to their un-barred and edge-on galaxy counterparts. Moreover, the majority of barred galaxies require multi-component surface brightness profiles in their best-fit models, indicative of the presence of resonance systems. We find that galaxies classed as barred with radial bar-driven flows (“barred + radial flow” subset) have significantly suppressed global star-formation efficiencies compared to barred galaxies without radial bar-driven flows and galaxies in the other morphological sub-samples. Our “barred + radial flow” subset galaxies also possess consistently centrally concentrated molecular gas distributions, with no indication of depleted gas mass fractions, suggesting that gas exhaustion is not the cause of their suppressed star formation. Furthermore, these objects have higher median gas mass surface densities in their central 1 kpc, implying that a central gas enhancements do not fuel central starbursts in these objects. We propose that dynamical effects, such as shear caused by large-scale inflows of gas, act to gravitationally stabilise the inner gas reservoirs

European registry on helicobacter pylori management: Effectiveness of first and second-line treatment in Spain

The management of Helicobacter pylori infection has to rely on previous local effectiveness due to the geographical variability of antibiotic resistance. The aim of this study was to evaluate the effectiveness of first and second-line H. pylori treatment in Spain, where the empirical prescription is recommended. A multicentre prospective non-interventional registry of the clinical practice of European gastroenterologists concerning H. pylori infection (Hp-EuReg) was developed, including patients from 2013 until June 2019. Effectiveness was evaluated descriptively and through a multivariate analysis concerning age, gender, presence of ulcer, proton-pump in-hibitor (PPI) dose, therapy duration and compliance. Overall, 53 Spanish hospitals were included, and 10, 267 patients received a first-line therapy. The best results were obtained with the 10-day bismuth single-capsule therapy (95% cure rate by intention-to-treat) and with both the 14-day bismuth-clarithromycin quadruple (PPI-bismuth-clarithromycin-amoxicillin, 91%) and the 14-day non-bismuth quadruple concomitant (PPI-clarithromycin-amoxicillin-metronidazole, 92%) therapies. Second-line therapies were prescribed to 2448 patients, with most-effective therapies being the triple quinolone (PPI-amoxicillin-levofloxacin/moxifloxacin) and the bismuth-levofloxacin quadruple schemes (PPI-bismuth-levofloxacin-amoxicillin) prescribed for 14 days (92%, 89% and 90% effective-ness, respectively), and the bismuth single-capsule (10 days, 88.5%). Compliance, longer duration and higher acid inhibition were associated with higher effectiveness. “Optimized” H. pylori therapies achieve over 90% success in Spain

Haploinsufficiency of EHMT1 improves pattern separation and increases hippocampal cell proliferation

Contains fulltext : 169681.pdf (publisher's version ) (Open Access)Heterozygous mutations or deletions of the human Euchromatin Histone Methyltransferase 1 (EHMT1) gene are the main causes of Kleefstra syndrome, a neurodevelopmental disorder that is characterized by impaired memory, autistic features and mostly severe intellectual disability. Previously, Ehmt1+/- heterozygous knockout mice were found to exhibit cranial abnormalities and decreased sociability, phenotypes similar to those observed in Kleefstra syndrome patients. In addition, Ehmt1+/- knockout mice were impaired at fear extinction and novel- and spatial object recognition. In this study, Ehmt1+/- and wild-type mice were tested on several cognitive tests in a touchscreen-equipped operant chamber to further investigate the nature of learning and memory changes. Performance of Ehmt1+/- mice in the Visual Discrimination &Reversal learning, object-location Paired-Associates learning- and Extinction learning tasks was found to be unimpaired. Remarkably, Ehmt1+/- mice showed enhanced performance on the Location Discrimination test of pattern separation. In line with improved Location Discrimination ability, an increase in BrdU-labelled cells in the subgranular zone of the dentate gyrus was observed. In conclusion, reduced levels of EHMT1 protein in Ehmt1+/- mice does not result in general learning deficits in a touchscreen-based battery, but leads to increased adult cell proliferation in the hippocampus and enhanced pattern separation ability