Synthesis of fused cyclic ethers in the search for hybrids hapten as promoters of ciguatera antibodies

Máster Universitario en QuímicaEn este trabajo, se ha realizado la síntesis de éteres cíclicos fusionados basada en la ciclación de Prins, utilizando sales de hierro(III) como catalizador, con el fin de encontrar haptenos, lo suficientemente activos para provocar una respuesta inmune frente a la ciguatoxina y permitir el desarrollo de anticuerpos contra la intoxicación por la ciguatera (CFP).In this work, the synthesis of fused cyclic ethers based on the Prins cyclization, using iron (III) salts as catalyst, has been performed in order to find haptens, active enough to cause an immune response against ciguatoxin and allow the development of antibodies against ciguatera fish poisoning (CFP

Isolation of streptomycetes causing common scab from 3-years old potato samples from South America

In this paper, we aimed at assessing the best conditions for the isolation of actinobacteria from old potato samples. A set of media and pretreatments were tested. The optimal were chosen for the isolation of actinobacteria from potatoes from Peru, Chile and Argentina. Isolates were tested on the presence of thaxtomin phytotoxin by amplification of the respective gene. Phylogenetic position of strains was compared with their geographical origin, pathogenic potential and existence of common scab (CS) symptoms on potato sample. We demonstrated that RNAlater can be successfully used for the long-term preservation of potato peel for subsequent isolation of actinobacteria on R2A medium. Many streptomycetes were thaxtomin-positive, though they are distantly-related to described pathogens causing CS. Genus Nocardia was first reported to be thaxtomin-positive. Potentially pathogenic strains were isolated not only from infected potato but also from those lacking CS symptoms. Some strains from scabby potatoes were thaxtomin-negative.EEA BalcarceFil: Rapoport, Daria. Charles University in Prague. Faculty opf Science, República Checa. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa.Fil: Patrmanova, T. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa.Fil: Kopecky, J. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa.Fil: Mareckova, M. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa. Czech University of Life Sciences, Faculty of Agrobiology, Food, and Natural Resources, Department of Microbiology, República Checa.Fil: Clemente, Gladys. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Salvalaggio, Andrea Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina

High-visibility interference fringes with femtosecond laser radiation

We propose and experimentally demonstrate an interferometer for femtosecond pulses with spectral bandwidth about 100 nm. The scheme is based on a Michelson interferometer with a dispersion compensating module. A diffractive lens serves the purpose of equalizing the optical-path-length difference for a wide range of frequencies. In this way, it is possible to register high-contrast interference fringes with micrometric resolution over the whole area of a commercial CCD sensor for broadband femtosecond pulse

Spatial-chirp compensation in dynamical holograms reconstructed with ultrafast lasers

A computer generated hologram (CGH) reconstructed with a sub-100-fs laser pulse at the focal plane of a conventional refractive lens experiences a large amount of spatial chirp. We report the shaping of a 12 fs laser pulsed beam by means of a Fourier CGH implemented onto a spatial light modulator, using a hybrid diffractive-refractive lens triplet that provides spatial-chirp compensation. Experimental results demonstrate that parallel, arbitrary, and high-resolution patterning is possible with the proposed devic

Molecular mechanisms underlying AMH elevation in hyperoestrogenic states in males

Anti-Müllerian hormone (AMH) is secreted by Sertoli cells of the testes from early fetal life until puberty, when it is downregulated by androgens. In conditions like complete androgen insensitivity syndrome (CAIS), AMH downregulation does not occur and AMH increases at puberty, due in part to follicle-stimulating hormone (FSH) effect. However, other conditions like Peutz-Jeghers syndrome (PJS), characterised by low FSH, also have increased AMH. Because both CAIS and PJS may present as hyperoestrogenic states, we tested the hypothesis that oestradiol (E2) upregulates AMH expression in peripubertal Sertoli cells and explored the molecular mechanisms potentially involved. The results showed that E2 is capable of inducing an upregulation of endogenous AMH and of the AMH promoter activity in the prepubertal Sertoli cell line SMAT1, signalling through ERα binding to a specific ERE sequence present on the hAMH promoter. A modest action was also mediated through the membrane oestrogen receptor GPER. Additionally, the existence of ERα expression in Sertoli cells in patients with CAIS was confirmed by immunohistochemistry. The evidence presented here provides biological plausibility to the hypothesis that testicular AMH production increases in clinical conditions in response to elevated oestrogen levels.Fil: Valeri, Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; ArgentinaFil: Lovaisa, María M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; ArgentinaFil: Racine, Chrystèle. Inserm; FranciaFil: Edelsztein, Nadia Yasmín. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; ArgentinaFil: Riggio, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Giulianelli, Sebastian Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; ArgentinaFil: Venara, Marcela Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; ArgentinaFil: Bedecarras, Patricia Gladys. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; ArgentinaFil: Ballerini, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; ArgentinaFil: di Clemente, Nathalie. Inserm; FranciaFil: Lamb, Caroline Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Schteingart, Helena Fedora. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; ArgentinaFil: Rey, Rodolfo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentin

The foreign policy of Colombia in the Caribbean Basin, 1832-1904

EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Estudio de las competencias en el grado de Enfermería del National University College de Puerto Rico desde la perspectiva de los alumnos, docentes y empleadores

Resumen: El trabajo que presentamos está relacionado con el estudio de las competencias genéricas y específicas desde la perspectiva europea (Proyecto Tuning Europa), la visión latinoamericana (Proyecto Tuning América Latina) y los requerimientos establecidos por la Accreditation Commission for Education in Nursing, así como de otras investigaciones actuales sobre competencias en Enfermería. Para realizar esta investigación, utilizamos una metodología ex post facto de tipo comparativo-causal, planteamos como objetivo de investigación determinar el grado de importancia que deben tener las competencias del grado profesional de Enfermería desde la opinión del alumnado, el profesorado y los empleadores y si dicha opinión muestra o no diferencias estadísticamente significativas. Elegimos una muestra en la que están representados los 3 agentes, elaborando 3 escalas en formato Likert. Cada una de las escalas está conformada por 22 competencias valoradas con 5 categorías de respuesta. Algunas de las conclusiones a las que llegamos con este trabajo son la combinación de criterios entre los alumnos, docentes y empleadores para poder establecer un compromiso para mejorar los planes de estudios del grado de Enfermería y su adecuación al ámbito laboral. Abstract: The work presented is related to the study of generic and specific competences from the European perspective (Tuning Project), the Latin American vision through (Tuning Latin America Project) and the requirements established by the Accreditation Commission for Education in Nursing, as well as, other current research on nursing skills. To carry out this research, we have used an ex post facto methodology, exactly a comparative-causal study, establishing as the main research objective the degree of importance that competences should have in the Nursing program from the students, faculty members and employers point of view, and if their opinions shows or not statistically significant differences. For the study, we chose a sample in which the 3 agents are represented and 3 Likert scales were designed. Each scale is comprised of 22 competencies which have been valued with 5 categories of response. Some conclusions of this study are the combination of criteria among the students, teachers and employers, in order to establish a commitment to improve the curriculum of the degree of Nursing and its adaptation to the workplace. Palabras clave: Competencias, Enfermería, Tuning, Alumnado, Docentes, Empleadores, Keywords: Competencies, Nursing, Tuning, Students, Teachers, Employer

Preformulation Studies of Novel Menthol Prodrugs with Antiparasitic Activity: Chemical Stability, In Silico, and In Vitro Permeability Assays

Based on the demonstrated and reported trypanocidal, leishmanicidal, and antiplasmodial activities of two menthol prodrugs, it was decided to proceed with preformulation studies, which are of key relevance in the drug discovery process. The aim of this study is to examine the stability and permeability of two new menthol prodrugs with antiparasitic activity. To determine the stability of menthol and its prodrugs, the corresponding studies were carried out in buffered solutions at pH values of 1.2, 5.8, and 7.4 at 37 °C. In silico permeability studies were performed using the free PerMM software and then in vitro permeability studies were performed using a biomimetic artificial membrane (BAM). Permeability studies conducted in silico predicted that both menthol and its prodrugs would pass through biological membranes via flip-flop movement. This prediction was subsequently confirmed by in vitro BAM permeability studies, where it was observed that the menthol prodrugs (1c and 1g) exhibited the highest Papp (apparent permeability) value compared to the parent compound. The study reveals that menthol prodrugs exhibit stability at a pH of 5.8 and possess sufficient in vitro permeability values as preformulation parameters

Planeamiento estratégico para la región Huancavelica

El plan estratégico de la región Huancavelica se ha desarrollado considerando una situación futura deseada que haya superado los altos índices de pobreza a través de la explotación de sus principales actividades productivas, buscando como fin máximo el bienestar social en la población. Para esto, se ha desarrollado una auditoría externa de la región para identificar los factores claves de éxito que permitan tener claro aquellas amenazas del entorno para aprovechar los recursos propios y hacerles frente, así como las oportunidades que ofrece el mercado resumidas en la demanda de productos a nivel global. Del mismo modo se procedió a realizar de manera exhaustiva una auditoria interna para identificar aquellas debilidades propias de la región a fin de mejorarlas, así como aquellas fortalezas que serán el principal recurso para definir las estrategias y así llegar a la situación futura deseada. Conociendo el panorama general de la región, tanto interna como externamente, se definió los objetivos a largo plazo que consideran la explotación sostenible e intensiva de las industrias de la trucha, fibra de alpaca y la palta, lo cual mejorará el bienestar social de la población a través de la generación de empleos adecuados. Para esto fue necesario formular las estrategias que permitan conseguir los objetivos planteados. Estas estrategias externas e internas cuidadosamente analizadas y seleccionadas serán parte de los objetivos a corto plazo, los cuales serán evaluados mediante el Tablero de Control Balanceado. El planteamiento de las estrategias está sustentado en la creciente demanda del mercado externo, para lo cual se implementarán estrategias de desarrollo y penetración de mercados; mientras que para convertir las debilidades en fortalezas se aplicarán estrategias de aventura conjunta, así como estrategias internas como gerencia de procesos y reingeniería. Con todo esto se espera mejorar la competitividad de la región que a la fecha se encuentra posicionada entre las últimas del Perú.The strategic plan of Huancavelica region has been developed considering a desired future situation that has overcome the high poverty rates through the exploitation of its main productive activities, looking for the social welfare in the population as a maximum goal. For this, an external audit of the region has been developed to identify the key success factors that make it possible to be clear about external threats to take advantage of own resources and to face them, as well as the opportunities offered by the market summarized in the demand for products globally. In the same way, an internal audit was carried out exhaustively to identify those weaknesses specific to the region in order to improve them, as those strengths that will be the main resource to define the strategies and thus reach the desired future situation. Knowing the general panorama of the region, both internally and externally, the long-term objectives that consider the sustainable and intensive exploitation of the industries of trout fillet, alpaca fiber and Hass avocado were defined, which will improve the social welfare of the population through the generation of adequate jobs. For this it was necessary to formulate the strategies that allow achieving the proposed objectives. These external and internal strategies carefully analyzed and selected will be part of the short-term objectives, which will be evaluated through the Balanced Control Board. The approach of the strategies is based on the growing demand of the external market, for which market development and penetration strategies will be implemented; while to convert the weaknesses into strengths, joint adventure strategies will be applied, as well as internal strategies such as process management and reengineering. With all this we hope to improve the competitiveness of the region that is among the last in Peru so that it can continue its development and growth in a sustainable manner.Tesi

Multisectoral Awareness and Acceptability of the VMGO and Meaning Making of the Vision and Mission

An institution's programs, activities, initiatives, and other institutionally-related undertakings would be transformed into the most renowned globally-acknowledged worldwide standards in light of the vision-mission. The survey on the awareness and acceptability of the University's Revised Vision-Mission, as well as the College of Teacher Education and Graduate School Program Goals and Program Objectives and Outcomes, demonstrates the awareness and acceptability of the University's Vision, Mission, Goals, and Objectives, as well as the University Core Values. It also includes translations of the new vision and mission from English to Vietnamese, Korean, Filipino, Ilocano, and Ibanag by the statekholders of the new vision and purpose. Significantly, by translating the VGMO, the university is expanding worldwide, emphasizing the importance of academic and community effort as it materializes the programs that will propel the institution forward