Spectroscopic detection of CIV in a galaxy at z=7.045: Implications for the ionizing spectra of reionization-era galaxies

We present Keck/MOSFIRE observations of UV metal lines in four bright gravitationally-lensed z~6-8 galaxies behind the cluster Abell 1703. The spectrum of A1703-zd6, a highly-magnified star forming galaxy with a Lyman-alpha redshift of z=7.045, reveals a confident detection of the nebular CIV emission line (unresolved with FWHM < 125 km/s). UV metal lines are not detected in the three other galaxies. At z~2-3, nebular CIV emission is observed in just 1% of UV-selected galaxies. The presence of strong CIV emission in one of the small sample of galaxies targeted in this paper may indicate hard ionizing spectra are more common at z~7. The total estimated equivalent width of the CIV doublet (38 A) and CIV/Lyman-alpha flux ratio (0.3) are comparable to measurements of narrow-lined AGNs. Photoionization models show that the nebular CIV line can also be reproduced by a young stellar population, with very hot metal poor stars dominating the photon flux responsible for triply ionizing carbon. Regardless of the origin of the CIV, we show that the ionizing spectrum of A1703-zd6 is different from that of typical galaxies at z~2, producing more H ionizing photons per unit 1500A luminosity and a larger flux density at 30-50 eV. If such extreme radiation fields are typical in UV-selected systems at z>7, it would indicate that reionization-era galaxies are more efficient ionizing agents than previously thought. Alternatively, we suggest that the small sample of Lyman-alpha emitters at z>7 may trace a rare population with intense radiation fields capable of ionizing their surrounding hydrogen distribution. Additional constraints on high ionization emission lines in galaxies with and without Lyman-alpha detections will help clarify whether hard ionizing spectra are common in the reionization era.Comment: 11 pages, 6 figures, submitted to MNRA

'Recruitment, recruitment, recruitment' - the need for more focus on retention: a qualitative study of five trials

Abstract Background Loss to follow-up (attrition) is a frequent problem in clinical trials and can introduce bias or reduce power. So, understanding retention issues and strategies to address these are important. As part of a multi-method project, this qualitative study aimed to explore retention strategies used by trial teams and factors which may influence strategy adoption. Method A purposive sample of active trials was selected from the UK NIHR HTA portfolio of ongoing trials in 2014/2015. Semi-structured interviews with several trial team members from each trial and supplementary interviews with experienced trial managers explored strategies in collecting clinical outcome data and retaining participants. Interview data were analysed thematically using techniques of constant comparison. Results Twenty-two semi-structured interviews with trial team members including chief investigators, trial managers, nurses and research administrators revealed strategies used to enhance retention. Some were recognised methods and planned from trial outset whilst others were implemented more responsively. Interviewees placed great value on fostering positive relationships with trial participants to enhance retention. However, these strategies took time which was not always appreciated by the wider trial team or funding bodies. The national focus on recruitment targets in networks posed a challenge to staff and was deemed detrimental to retention. The ‘moral compass’ of individual researchers relied on their own beliefs and values and research experience and the factors affected their confidence to pursue participant data during follow-up. Conclusion The role of trial staff and their underlying behaviours influence retention practices and, combined with emphasis on recruitment targets, can be detrimental to motivation and retention activities. There is a need to consider how to train and support trial staff involved in retention practices and recognition of retention from funding bodies and oversight organisations

Ferromagnetism in an extended coherently-coupled atomic superfluid

We investigate an extended atomic superfluid in the presence of a coherent coupling between two internal states and find that it exhibits a quantum phase transition from a para- to a ferromagnetic state. The nature of the transition is experimentally assessed by looking at the phase diagram as a function of the control parameters, at hysteresis phenomena, and at the magnetic susceptibility and the magnetization fluctuations around the critical point. Agreement with mean-field calculations is shown. We develop experimental protocols to deterministically generate domain walls that separate spatial regions of opposite magnetization in the ferromagnetic state. Thanks to the enhanced coherence properties of our atomic superfluid system compared to standard condensed matter systems, our results open the way towards the study of different aspects of the relaxation dynamics in isolated coherent many-body quantum systems.Comment: 14 pages, 6 figure

Plasmodium falciparum Produce Lower Infection Intensities in Local versus Foreign Anopheles gambiae Populations

Both Plasmodium falciparum and Anopheles gambiae show great diversity in Africa, in their own genetic makeup and population dynamics. The genetics of the individual mosquito and parasite are known to play a role in determining the outcome of infection in the vector, but whether differences in infection phenotype vary between populations remains to be investigated. Here we established two A. gambiae s.s. M molecular form colonies from Cameroon and Burkina Faso, representing a local and a foreign population for each of the geographical sites. Experimental infections of both colonies were conducted in Cameroon and Burkina Faso using local wild P. falciparum, giving a sympatric and allopatric vector-parasite combination in each site. Infection phenotype was determined in terms of oocyst prevalence and intensity for at least nine infections for each vector-parasite combination. Sympatric infections were found to produce 25% fewer oocysts per midgut than allopatric infections, while prevalence was not affected by local/foreign interactions. The reduction in oocyst numbers in sympatric couples may be the result of evolutionary processes where the mosquito populations have locally adapted to their parasite populations. Future research on vector-parasite interactions must take into account the geographic scale of adaptation revealed here by conducting experiments in natural sympatric populations to give epidemiologically meaningful results

Prevalence of onchocerciasis in the Fundong Health District, Cameroon after 6 years of continuous community-directed treatment with ivermectin

<0.001). The greatest rate of infection was found among farmers (2.5%) followed by students (0.7%) and businessmen (0.25%). CONCLUSION: This study shows that the study area is now hypo-endemic for onchocerciasis, following 6 years of continuous treatment with ivermectin. Careful monitoring of onchocerciasis should however be continued to avoid that the area returns to its initial hyper endemicity

Natural plant diet impacts phenotypic expression of pyrethroid resistance in Anopheles mosquitoes

Success in reducing malaria transmission through vector control is threatened by insecticide resistance in mosquitoes. Although the proximal molecular mechanisms and genetic determinants involved are well documented, little is known about the influence of the environment on mosquito resistance to insecticides. The aim of this study was to assess the effect of plant sugar feeding on the response of Anopheles gambiae sensu lato to insecticides. Adults were fed with one of four treatments, namely a 5% glucose control solution, nectariferous flowers of Barleria lupulina, of Cascabela thevetia and a combination of both B. lupulina+C. thevetia. WHO tube tests were performed with 0.05% and 0.5% deltamethrin, and knockdown rate (KD) and the 24 h mosquito mortality were measured. Plant diet significantly influenced mosquito KD rate at both concentrations of deltamethrin. Following exposure to 0.05% deltamethrin, the B. lupulina diet induced a 2.5 fold-increase in mosquito mortality compared to 5% glucose. Species molecular identification confirmed the predominance of An. gambiae (60% of the samples) over An. coluzzii and An. arabiensis in our study area. The kdr mutation L1014F displayed an allelic frequency of 0.75 and was positively associated with increased phenotypic resistance to deltamethrin. Plant diet, particularly B. lupulina, increased the susceptibility of mosquitoes to insecticides. The finding that B. lupulina-fed control individuals (i.e. not exposed to deltamethrin) also displayed increased 24 h mortality suggests that plant-mediated effects may be driven by a direct effect of plant diet on mosquito survival rather than indirect effects through interference with insecticide-resistance mechanisms. Thus, some plant species may weaken mosquitoes, making them less vigorous and more vulnerable to the insecticide. There is a need for further investigation, using a wider range of plant species and insecticides, in combination with other relevant environmental factors, to better understand the expression and evolution of insecticide resistance

Diagnostic applications of next generation sequencing: working towards quality standards

Over the past 6 years, next generation sequencing (NGS) has been established as a valuable high-throughput method for research in molecular genetics and has successfully been employed in the identification of rare and common genetic variations. All major NGS technology companies providing commercially available instruments (Roche 454, Illumina, Life Technologies) have recently marketed bench top sequencing instruments with lower throughput and shorter run times, thereby broadening the applications of NGS and opening the technology to the potential use for clinical diagnostics. Although the high expectations regarding the discovery of new diagnostic targets and an overall reduction of cost have been achieved, technological challenges in instrument handling, robustness of the chemistry and data analysis need to be overcome. To facilitate the implementation of NGS as a routine method in molecular diagnostics, consistent quality standards need to be developed. Here the authors give an overview of the current standards in protocols and workflows and discuss possible approaches to define quality criteria for NGS in molecular genetic diagnostics