Estimating Northern Bobwhite Density in Privately-Owned Forests Across the Southeast

Rigorous density estimates can inform management, conservation planning, and policy decisions. Northern bobwhite (Colinus virginianus; hereafter, bobwhite) populations are declining throughout their range, including the southeastern United States. Numerous private land conservation initiatives are underway to restore bobwhite populations in these areas, but baseline estimates of density spanning privately owned forests are sparse. This information gap makes it difficult to evaluate the population-level effects of these conservation programs or develop expectations for timelines to reach population targets. We sought to understand baseline densities across privately owned pine forests in the southeastern United States in areas targeted for bobwhite conservation. We sampled 138 pine stands a total of 286 times across Alabama, Florida, Georgia, North Carolina, and South Carolina from 2018 to 2020 using autumn distance sampling point counts. We sampled 110 stands that were either planned or applied U.S. Department of Agriculture, Natural Resources Conservation Service (NRCS) management contracts, specifically Working Lands for Wildlife (WLFW) Northern Bobwhite partnerships or similar Environmental Quality Incentives Program contracts. The WLFW contracts were solely in focal counties identified in the National Bobwhite Conservation Initiative 2.0 plan and by cooperating state wildlife agencies. Contracts were on average 17.4 ha (SD = 27.0). We also sampled 28 areas with extensive (\u3e200 ha) and intensive (e.g., at least every 3 years for the last ~10 years) habitat management for bobwhite. We used a hierarchical distance sampling model to estimate density. Density was lowest in North Carolina and South Carolina. Densities in Alabama were slightly greater, and greatest densities were in Georgia and Florida. However, the majority of observed densities were 2 coveys/40 ha or below, and all were below 4 coveys/40 ha. Large and intensively managed sites on average had 4 times greater density compared to smaller contracts. Specific densities will be reported in the future. Our sampling efforts fill an important information gap regarding densities throughout private lands in the southeastern United States. Our models indicate that bobwhite densities are generally low in pine forests of the Southeast even in counties identified as having the greatest conservation potential. The relatively greater densities at large and intensively managed sites illustrate the need for more intensive landscape-scale planning when implementing private land conservation. Pending research will compare densities at planned and applied NRCS contracts in the context of landscape-scale habitat amount

Modified bacterial reaction centers

Pigments of borohydride-treated reaction centers of Rhodobacter sphaeroides R 26 and Rhodopseudomonas viridis were analyzed by HPLC with polychromatic detection. In both species, pigment composition and contents were unchanged. Reaction centers from Rhodobacter sphaeroides R26 were prepared in which bacteriochlorophylls (BA,B) and bacteriopheophytins (HA,B) were exchanged with their potential borohydride products reduced at C-31. [3-Hydroxyethyl]-BChl a exchanges selectively into the BA,B pockets, and 31-OH-BPh a to the HA,B pockets. Stable reaction centers are obtained in both cases. A comparison of the absorption and circular dichroism spectra of reaction centers after exchange with 31-OH pigments, and of borohydride-modified reaction centers, reveal distinct differences. It is concluded that during borohydride reduction none of the pigments is chemically modified or extracted from the reaction centers

Adolescent and Young Adult Substance Use: Association with Sensation Seeking, Self Esteem and Retrospective Report of Early Pubertal Onset. A Preliminary Examination

Structured questionnaires were administered to investigate the relationship between early pubertal onset, substance abuse, sensation seeking, and self-esteem. The current study presents data from 1,002 subjects, who were followed from the 6th to the 10th grades and again at the age of 20. In females, early pubertal onset was associated with greater cigarette use and lower self-esteem. Further the interaction of early pubertal onset and low self-esteem was predictive for cigarette use in females, as was early pubertal onset and high sensation seeking. Late pubertal onset was associated with decreased alcohol use and lower sensation seeking in males, with the opposite trends for early pubertal onset. These interrelationships must be considered when attempting to understand and identify early adolescent initiation of substance abuse

Pharmacy Staff Perspectives on Alcohol and Medication Interaction Prevention Among Older Rural Adults

Older adults are at high risk for alcohol and medication interactions (AMI). Pharmacies have the potential to act as ideal locations for AMI education, as pharmacy staff play an important role in the community. This study examined the perspectives of pharmacy staff on AMI prevention programming messaging, potential barriers to and facilitators of older adult participation in such programming, and dissemination methods for AMI prevention information. Flyers, telephone calls, and site visits were used to recruit 31 pharmacy staff members who participated in semistructured interviews. A content analysis of interview transcriptions was conducted to identify major themes, categories, and subcategories. The main categories identified for AMI prevention messaging were Informational, Health Significance, and Recommendations. Within barriers to participation, the main categories identified were Health Illiteracy, Personal Attitudes, and Feasibility. The main categories identified for program facilitators were Understanding, Beneficial Consequences, and Practicality. Multimethod dissemination strategies were commonly suggested. This study found positive pharmacy staff perspectives for the planning and implementation of AMI prevention programming, and future development and feasibility testing of such programming in the pharmacy setting is warranted

Pharmacy Staff Perspectives on Alcohol and Medication Interaction Prevention Among Older Rural Adults

Older adults are at high risk for alcohol and medication interactions (AMI). Pharmacies have the potential to act as ideal locations for AMI education, as pharmacy staff play an important role in the community. This study examined the perspectives of pharmacy staff on AMI prevention programming messaging, potential barriers to and facilitators of older adult participation in such programming, and dissemination methods for AMI prevention information. Flyers, telephone calls, and site visits were used to recruit 31 pharmacy staff members who participated in semistructured interviews. A content analysis of interview transcriptions was conducted to identify major themes, categories, and subcategories. The main categories identified for AMI prevention messaging were Informational, Health Significance, and Recommendations. Within barriers to participation, the main categories identified were Health Illiteracy, Personal Attitudes, and Feasibility. The main categories identified for program facilitators were Understanding, Beneficial Consequences, and Practicality. Multimethod dissemination strategies were commonly suggested. This study found positive pharmacy staff perspectives for the planning and implementation of AMI prevention programming, and future development and feasibility testing of such programming in the pharmacy setting is warranted

The regulation of miRNAs by reconstituted high-density lipoproteins in diabetes-impaired angiogenesis

Diabetic vascular complications are associated with impaired ischaemia-driven angiogenesis. We recently found that reconstituted high-density lipoproteins (rHDL) rescue diabetes-impaired angiogenesis. microRNAs (miRNAs) regulate angiogenesis and are transported within HDL to sites of injury/repair. The role of miRNAs in the rescue of diabetes-impaired angiogenesis by rHDL is unknown. Using a miRNA array, we found that rHDL inhibits hsa-miR-181c-5p expression in vitro and using a hsa-miR-181c-5p mimic and antimiR identify a novel anti-angiogenic role for miR-181c-5p. miRNA expression was tracked over time post-hindlimb ischaemic induction in diabetic mice. Early post-ischaemia when angiogenesis is important, rHDL suppressed hindlimb mmu-miR-181c-5p. mmu-miR-181c-5p was not detected in the plasma or within HDL, suggesting rHDL specifically targets mmu-miR-181c-5p at the ischaemic site. Three known angiogenic miRNAs (mmu-miR-223-3p, mmu-miR-27b-3p, mmu-miR-92a-3p) were elevated in the HDL fraction of diabetic rHDL-infused mice early post-ischaemia. This was accompanied by a decrease in plasma levels. Only mmu-miR-223-3p levels were elevated in the hindlimb 3 days post-ischaemia, indicating that rHDL regulates mmu-miR-223-3p in a time-dependent and site-specific manner. The early regulation of miRNAs, particularly miR-181c-5p, may underpin the rescue of diabetes-impaired angiogenesis by rHDL and has implications for the treatment of diabetes-related vascular complications

The Role of Polycyclic Aromatic Hydrocarbons in Ultraviolet Extinction. I. Probing small molecular PAHs

We have obtained new STIS/HST spectra to search for structure in the ultraviolet interstellar extinction curve, with particular emphasis on a search for absorption features produced by polycyclic aromatic hydrocarbons (PAHs). The presence of these molecules in the interstellar medium has been postulated to explain the infrared emission features seen in the 3-13 $\mu$m spectra of numerous sources. UV spectra are uniquely capable of identifying specific PAH molecules. We obtained high S/N UV spectra of stars which are significantly more reddened than those observed in previous studies. These data put limits on the role of small (30-50 carbon atoms) PAHs in UV extinction and call for further observations to probe the role of larger PAHs. PAHs are of importance because of their ubiquity and high abundance inferred from the infrared data and also because they may link the molecular and dust phases of the interstellar medium. A presence or absence of ultraviolet absorption bands due to PAHs could be a definitive test of this hypothesis. We should be able to detect a 20 \AA wide feature down to a 3$\sigma$ limit of $\sim$0.02 A$_V$. No such absorption features are seen other than the well-known 2175 \AA bump.Comment: 16 pages, 3 figure, ApJ in pres

Dust Grain-Size Distributions From MRN to MEM

Employing the Maximum Entropy Method algorithm, we fit interstellar extinction measurements which span the wavelength range 0.125-3 micron. We present a uniform set of MEM model fits, all using the same grain materials, optical constants and abundance constraints. In addition, we are taking advantage of improved UV and IR data and better estimates of the gas-to-dust ratio. The model fits cover the entire range of extinction properties that have been seen in the Galaxy and the Magellanic Clouds. The grain models employed for this presentation are the simplistic homogeneous spheres models (i.e., Mathis, Rumpl, & Nordsieck 1977) with two (graphite, silicate) or three (graphite, silicate, amorphous carbon) components. Though such usage is only a first step, the results do provide interesting insight into the use of grain size as a diagnostic of dust environment. We find that the SMC Bar extinction curve cannot be fit using carbon grains alone. This is a challenge to the recent observational result indicating little silicon depletion in the SMC.Comment: 24 pages, 5 figures, accepted for publication in the Astrophysical Journa

Lifting the Veil of Dust from NGC 0959: The Importance of a Pixel-Based 2D Extinction Correction

We present the results of a study of the late-type spiral galaxy NGC 0959, before and after application of the pixel-based dust extinction correction described in Tamura et al. 2009 (Paper I). Galaxy Evolution Explorer (GALEX) far-UV (FUV) and near-UV (NUV), ground-based Vatican Advanced Technology Telescope (VATT) UBVR, and Spitzer/Infrared Array Camera (IRAC) 3.6, 4.5, 5.8, and 8.0 micron images are studied through pixel Color-Magnitude Diagrams (pCMDs) and pixel Color-Color Diagrams (pCCDs). We define groups of pixels based on their distribution in a pCCD of (B - 3.6 micron) versus (FUV - U) colors after extinction correction. In the same pCCD, we trace their locations before the extinction correction was applied. This shows that selecting pixel groups is not meaningful when using colors uncorrected for dust. We also trace the distribution of the pixel groups on a pixel coordinate map of the galaxy. We find that the pixel-based (two-dimensional) extinction correction is crucial to reveal the spatial variations in the dominant stellar population, averaged over each resolution element. Different types and mixtures of stellar populations, and galaxy structures such as a previously unrecognized bar, become readily discernible in the extinction-corrected pCCD and as coherent spatial structures in the pixel coordinate map.Comment: 10 pages, LaTeX2e requires 'emulateapj.cls', 'graphicx.sty', and 'natbib.sty' (included), 9 postscript figures, 1 table. Accepted for publication in AJ

FUSE Measurements of Far Ultraviolet Extinction. I. Galactic Sight Lines

We present extinction curves that include data down to far ultraviolet wavelengths (FUV; 1050 - 1200 A) for nine Galactic sight lines. The FUV extinction was measured using data from the Far Ultraviolet Spectroscopic Explorer. The sight lines were chosen for their unusual extinction properties in the infrared through the ultraviolet; that they probe a wide range of dust environments is evidenced by the large spread in their measured ratios of total-to-selective extinction, R_V = 2.43 - 3.81. We find that extrapolation of the Fitzpatrick & Massa relationship from the ultraviolet appears to be a good predictor of the FUV extinction behavior. We find that predictions of the FUV extinction based upon the Cardelli, Clayton & Mathis (CCM) dependence on R_V give mixed results. For the seven extinction curves well represented by CCM in the infrared through ultraviolet, the FUV extinction is well predicted in three sight lines, over-predicted in two sight lines, and under-predicted in 2 sight lines. A Maximum Entropy Method analysis using a simple three component grain model shows that seven of the nine sight lines in the study require a larger fraction of grain materials to be in dust when FUV extinction is included in the models. Most of the added grain material is in the form of small (radii < 200 A) grains.Comment: Accepted for publication in the Astrophysical Journal. 31 pages with 7 figure