Quality and Nutritive Value of Mott Dwarf Elephantgrass Silage with Biological Additives

This experiment was conducted to study the effects of cellulase enzymes and lactic acid bacteria on the fermentation characteristics, chemical composition and nutritive value of Pennisetum purpureum cv Mott silage. Five kinds of preparation were tested: sugar molasses (control); sugar molasses and cellulase enzymes; cellulase enzymes; concentrate of lactic acid bacteria and sugar molasses and concentrate of lactic acid bacteria. Significant differences (P\u3c 0.05) were found in fermentation quality and chemical composition of control silage as compared to the biological additives treated silage. Addition of cellulase enzymes and lactic acid bacteria resulted in decrease of pH value, cell wall content and increased lactic acid concentration. The biological additives increased silage intake and daily live weight by growing steers

Tiller Dynamic of Guineagrass (\u3cem\u3ePanicum maximum\u3c/em\u3e) Under Defoliation

Guineagrass (Panicum maximum) is a perennial warm season bunchgrass native to Africa that has been introduced to many tropical areas. Its management is important because of its important role in animal production. Total production of herbage and the persistence of tufted grasses can be markedly reduced when the grass is defoliated too frequently and too intensively (Clavero, 1997). In order to determine the proper management for optimum production and long term persistence, it is important to study the effect of cutting practices on tiller dynamics of guineagrass

Bulk and surface plasmon polariton excitation in RuO2 for low-loss plasmonic applications in NIR

Transition-metal oxides, such as RuO2, offer an exciting alternative to conventional metals for metamaterials and plasmonic applications due to their low optical losses in the visible and near-infrared ranges. In this manuscript we report observation of optically excited surface plasmon polaritons (SPPs) and bulk plasmons in RuO2 thin films grown using DC reactive magnetron sputtering on glass and TiO2 (001) substrates. We show that both plasmon modes can exist simultaneously for the infrared region of the optical spectrum, while only the bulk plasmons are supported at higher optical frequencies. Finally, we demonstrate that the film properties can be tailored to favor excitation of either SPP or bulk plasmons. (C) 2012 Optical Society of Americ

Evaluation of Reservoir Inflows Using Semi-Distributed Hydrological Modeling Techniques: Application to the Esil and Moildy Rivers' Catchments in Kazakhstan

[EN] This study explores the potential application of the HBV model for simulating water inflow into the Astana reservoir in Kazakhstan. The Astana reservoir, with an area of 60.9 km2 and a capacity of 411 million m3, serves as a vital hydrotechnical structure for the regulation of long-term water resources. Positioned on the Esil River, this reservoir plays a crucial role in providing drinking water to Astana, the capital city of the Republic of Kazakhstan. This study presents a concise overview of the HBV model and includes hydrometeorological data as well as information from a digital elevation model constructed from altitude maps of the catchment areas of the Esil and Moildy rivers. To simulate the runoff from both rivers, the GAP optimization algorithm was employed. Model parameters were calibrated for the period spanning 1975 to 2020. The effectiveness of the model was evaluated using the Nash-Sutcliffe criterion, which confirms a strong correlation between the simulated runoff dynamics and observed data. This study's outcomes reveal the efficiency of the selected optimal parameters, particularly for the 2020-2022 timeframe, during which the HBV model's parameters were validated. Overall, the quality metrics achieved during both the calibration and validation periods are satisfactory, affirming the model's suitability for short-term runoff forecasts, especially during flood events. Furthermore, the model proves valuable for assessing potential shifts in river runoff patterns under the influence of climate change.Serikbay, NT.; Tillakarim, TA.; Rodrigo-Ilarri, J.; Rodrigo-Clavero, M.; Duskayev, KK. (2023). Evaluation of Reservoir Inflows Using Semi-Distributed Hydrological Modeling Techniques: Application to the Esil and Moildy Rivers' Catchments in Kazakhstan. Water. 15(16). https://doi.org/10.3390/w15162967151

A fitted numerical method for singularly perturbed parabolic reaction-diffusion problems

This paper treats a time-dependent singularly perturbed reaction-diffusion problem. We semidiscretize the problem in time by means of the classical backward Euler method. We develop a fitted operator finite difference method (FOFDM) to solve the resulting set of linear problems (one at each time level). We prove that the underlying fitted operator satisfies the maximum principle. This result is then used in the error analysis of the FOFDM. The method is shown to be first order convergent in time and second order convergent in space, uniformly with respect to the perturbation parameter. We test the method on several numerical examples to confirm our theoretical findings.Web of Scienc

Development of a multiplex assay for antibody detection in serum against pathogens affecting ruminants

Numerous infectious diseases impacting livestock impose an important economic burden and in some cases also represent a threat to humans and are classified as zoonoses. Some zoonotic diseases are transmitted by vectors and, due to complex environmental and socio‐economic factors, the distribution of many of these pathogens is changing, with increasing numbers being found in previously unaffected countries. Here, we developed a multiplex assay, based on a suspension microarray, able to detect specific antibodies to five important pathogens of livestock (three of them zoonotic) that are currently emerging in new geographical locations: Rift Valley fever virus (RVFV), Crimean‐Congo haemorrhagic fever virus (CCHFV), Schmallenberg virus (SBV), Bluetongue virus (BTV) and the bacteria complex Mycobacterium tuberculosis. Using the Luminex platform, polystyrene microspheres were coated with recombinant proteins from each of the five pathogens. The mix of microspheres was used for the simultaneous detection of antibodies against the five corresponding diseases affecting ruminants. The following panel of sera was included in the study: 50 sera from sheep experimentally infected with RVFV, 74 sera from calves and lambs vaccinated with SBV, 26 sera from cattle vaccinated with Mycobacterium bovis, 30 field sera from different species of ruminants infected with CCHFV and 88 calf sera infected with BTV. Finally, to determine its diagnostic specificity 220 field sera from Spanish farms free of the five diseases were assessed. All the sera were classified using commercial ELISAs specific for each disease, used in this study as the reference technique. The results showed the multiplex assay exhibited good performance characteristics with values of sensitivity ranging from 93% to 100% and of specificity ranging from 96% to 99% depending on the pathogen. This new tool allows the simultaneous detection of antibodies against five important pathogens, reducing the volume of sample needed and the time of analysis where these pathogens are usually tested individually

Reversible Light-Induced Dimerization of Secondary Face Azobenzene-Functionalized β-Cyclodextrin Derivatives

β-cyclodextrin (βCyD) derivatives equipped with aromatic appendages at the secondary face exhibit tailorable self-assembling capabilities. The aromatic modules can participate in inclusion phenomena and/or aromatic-aromatic interactions. Supramolecular species can thus form that, at their turn, can engage in further co-assembling with third components in a highly regulated manner; the design of nonviral gene delivery systems is an illustrative example. Endowing such systems with stimuli responsiveness while keeping diastereomeric purity and a low synthetic effort is a highly wanted advancement. Here, we show that an azobenzene moiety can be “clicked” to a single secondary O-2 position of βCyD affording 1,2,3-triazole-linked βCyD-azobenzene derivatives that undergo reversible light-controlled self-organization into dimers where the monomer components face their secondary rims. Their photoswitching and supramolecular properties have been thoroughly characterized by UV-vis absorption, induced circular dichroism, nuclear magnetic resonance, and computational techniques. As model processes, the formation of inclusion complexes between a water-soluble triazolylazobenzene derivative and βCyD as well as the assembly of native βCyD/βCyD-azobenzene derivative heterodimers have been investigated in parallel. The stability of the host-guest supramolecules has been challenged against the competitor guest adamantylamine and the decrease of the medium polarity using methanol-water mixtures. The collective data support that the E-configured βCyD-azobenzene derivatives, in aqueous solution, form dimers stabilized by the interplay of aromatic-aromatic and aromatic-βCyD cavity interactions after partial reciprocal inclusion. Photoswitching to the Z-isomer disrupts the dimers into monomeric species, offering opportunity for the spatiotemporal control of the organizational status by light.Ministerio de Ciencia e Innovación PID2019-105858RB-I00, PID2020-118403GB-I00, PID2020-118384GB-I00, PID2020-119130GB-I00Fondo Europeo de Desarrollo Regional PID2021-124247OB-C21Junta de Andalucía P20_00166, US-1380698, P12-FQM-1467Universidad de Sevilla FPU18/02922, FPU19/0436

Assessing the conservation value of waterbodies: the example of the Loire floodplain (France)

In recent decades, two of the main management tools used to stem biodiversity erosion have been biodiversity monitoring and the conservation of natural areas. However, socio-economic pressure means that it is not usually possible to preserve the entire landscape, and so the rational prioritisation of sites has become a crucial issue. In this context, and because floodplains are one of the most threatened ecosystems, we propose a statistical strategy for evaluating conservation value, and used it to prioritise 46 waterbodies in the Loire floodplain (France). We began by determining a synthetic conservation index of fish communities (Q) for each waterbody. This synthetic index includes a conservation status index, an origin index, a rarity index and a richness index. We divided the waterbodies into 6 clusters with distinct structures of the basic indices. One of these clusters, with high Q median value, indicated that 4 waterbodies are important for fish biodiversity conservation. Conversely, two clusters with low Q median values included 11 waterbodies where restoration is called for. The results picked out high connectivity levels and low abundance of aquatic vegetation as the two main environmental characteristics of waterbodies with high conservation value. In addition, assessing the biodiversity and conservation value of territories using our multi-index approach plus an a posteriori hierarchical classification methodology reveals two major interests: (i) a possible geographical extension and (ii) a multi-taxa adaptation