The stellar content of the super star clusters in NGC 1569

We discuss HST FOS ultraviolet spectroscopy and NICMOS near-infrared photometry of four young super star clusters in the central region of the irregular starburst galaxy NGC 1569. The new observations coupled with previous HST WFPC2 photometry and ground-based optical spectroscopy allow us to isolate and age-date the hot and cool stellar components of these massive clusters. We analyze the two components A1 and A2 of the brightest super star cluster NGC 1569-A. This cluster received previous attention due to the simultaneous presence of Wolf-Rayet stars and red supergiants. The FOS spectra provide the first evidence for O-stars in NGC 1569-A, indicating a young (5 Myr) stellar component in A1 and/or A2. Comparison with other high-mass star-forming regions suggests that the O- and Wolf-Rayet stars are spatially coincident. If so, cluster A2 could be the host of the very young O- and Wolf-Rayet population, and the somewhat older red supergiants could be predominantly located in A1. The mass-to-light ratio of NGC 1569-A1 is analyzed in five optical and infrared photometric bands and compared to evolutionary synthesis models. No indications for an anomalous initial mass function are found, consistent with a scenario where this cluster is the progenitor of present-day globular clusters. The clusters A1 and A2 are compared to clusters B and #30. The latter two clusters are older and fully dominated by red supergiants. All four super star clusters provide a significant fraction (20 - 25%) of the total optical and near-infrared light in the central region of the galaxy. The photometric properties of the super star cluster population in NGC 1569 resemble those of the populous clusters in the Magellanic Clouds

Soil pH effects on the comparative toxicity of dissolved zinc, non-nano and nano ZnO to the earthworm Eisenia fetida

To determine how soil properties influence nanoparticle (NP) fate, bioavailability and toxicity, this study compared the toxicity of nano zinc oxide (ZnO NPs), non-nano ZnO and ionic ZnCl2 to the earthworm Eisenia fetida in a natural soil at three pH levels. NP characterisation indicated that reaction with the soil media greatly controls ZnO properties. Three main conclusions were drawn. First that Zn toxicity, especially for reproduction, was influenced by pH for all Zn forms. This can be linked to the influence of pH on Zn dissolution. Secondly, that ZnO fate, toxicity and bioaccumulation were similar (including relationships with pH) for both ZnO forms, indicating the absence of NP-specific effects. Finally, earthworm Zn concentrations were higher in worms exposed to ZnO compared to ZnCl2, despite the greater toxicity of the ionic form. This observation suggests the importance of considering the relationship between uptake and toxicity in nanotoxicology studies

Isometric Embedding of BPS Branes in Flat Spaces with Two Times

We show how non-near horizon p-brane theories can be obtained from two embedding constraints in a flat higher dimensional space with 2 time directions. In particular this includes the construction of D3 branes from a flat 12-dimensional action, and M2 and M5 branes from 13 dimensions. The worldvolume actions are determined by constant forms in the higher dimension, reduced to the usual expressions by Lagrange multipliers. The formulation affords insight in the global aspects of the spacetime geometries and makes contact with recent work on two-time physics.Comment: 29 pages, 10 figures, Latex using epsf.sty and here.sty; v2: reference added and some small correction

Water-soluble, stable and azide-reactive strained dialkynes for biocompatible double strain-promoted click chemistry.

The Sondheimer dialkyne is extensively used in double strain-promoted azide-alkyne cycloadditions. This reagent suffers with poor water-solubility and rapidly decomposes in aqueous solutions. This intrinsically limits its application in biological systems, and no effective solutions are currently available. Herein, we report the development of novel highly water-soluble, stable, and azide-reactive strained dialkyne reagents. To demonstrate their extensive utility, we applied our novel dialkynes to a double strain-promoted macrocyclisation strategy to generate functionalised p53-based stapled peptides for inhibiting the oncogenic p53-MDM2 interaction. These functionalised stapled peptides bind MDM2 with low nanomolar affinity and show p53 activation in a cellular environment. Overall, our highly soluble, stable and azide-reactive dialkynes offer significant advantages over the currently used Sondheimer dialkyne, and could be utilised for numerous biological applications

Serum neurofilament light chain – A potential biomarker for polyneuropathy in type 2 diabetes?

AimsTo investigate the relationship between neurofilament light chain (NfL) and the presence and severity of diabetic polyneuropathy (DPN).MethodsWe performed cross-sectional analysis of data from 178 participants of the ADDITION-Denmark cohort of people with screen-detected type 2 diabetes and 32 healthy controls. Biobank serum samples were analyzed for NfL using single-molecule array. DPN was defined by Toronto criteria for confirmed DPN. Original and axonal nerve conduction study (NCS) sum z-scores were used as indicators of the severity of DPN and peripheral nerve damage.Results39 (21.9%) participants had DPN. Serum NfL (s-NfL) was significantly higher in participants with DPN (18.8 ng/L [IQR 14.4; 27.9]) than in participants without DPN (15.4 ng/L [IQR 11.7; 20.1]). There were no unadjusted s-NfL differences between controls (17.6 ng/L [IQR 12.7; 19.8]) and participants with or without DPN. Higher original and axonal NCS sum z-scores were associated with 10% higher s-NfL (10.2 and 12.1% [95% CI’s 4.0; 16.8 and 6.6; 17.9] per 1 SD). The AUC of s-NfL for DPN was 0.63 (95% CI 0.52; 0.73).ConclusionsS-NfL is unlikely to be a reliable biomarker for the presence of DPN. S-NfL is however associated to the severity of the nerve damage underlying DPN

Genetic Variation in ABCC4 and CFTR and Acute Pancreatitis during Treatment of Pediatric Acute Lymphoblastic Leukemia

Background: Acute pancreatitis (AP) is a serious, mechanistically not entirely resolved side effect of L-asparaginase-containing treatment for acute lymphoblastic leukemia (ALL). To find new candidate variations for AP, we conducted a genome-wide association study (GWAS). Methods: In all, 1,004,623 single-nucleotide variants (SNVs) were analyzed in 51 pediatric ALL patients with AP (cases) and 1388 patients without AP (controls). Replication used independent patients. Results: The top-ranked SNV (rs4148513) was located within the ABCC4 gene (odds ratio (OR) 84.1; p = 1.04 × 10−14). Independent replication of our 20 top SNVs was not supportive of initial results, partly because rare variants were neither present in cases nor present in controls. However, results of combined analysis (GWAS and replication cohorts) remained significant (e.g., rs4148513; OR = 47.2; p = 7.31 × 10−9). Subsequently, we sequenced the entire ABCC4 gene and its close relative, the cystic fibrosis associated CFTR gene, a strong AP candidate gene, in 48 cases and 47 controls. Six AP-associated variants in ABCC4 and one variant in CFTR were detected. Replication confirmed the six ABCC4 variants but not the CFTR variant. Conclusions: Genetic variation within the ABCC4 gene was associated with AP during the treatment of ALL. No association of AP with CFTR was observed. Larger international studies are necessary to more conclusively assess the risk of rare clinical phenotypes

Airborne dust and high temperatures are risk factors for invasive bacterial disease

Background The Sahel region of West Africa has the highest bacterial meningitis attack and case fatality rate in the world. The effect of climatic factors on patterns of invasive respiratory bacterial disease is not well documented. Objective We aimed to assess the link between climatic factors and occurrence of invasive respiratory bacterial disease in a Sahel region of Niger. Methods We conducted daily disease surveillance and climatic monitoring over an 8-year period between January 1, 2003, and December 31, 2010, in Niamey, Niger, to determine risk factors for bacterial meningitis and invasive bacterial disease. We investigated the mechanistic effects of these factors on Streptococcus pneumoniae infection in mice. Results High temperatures and low visibility (resulting from high concentrations of airborne dust) were identified as significant risk factors for bacterial meningitis. Dust inhalation or exposure to high temperatures promoted progression of stable asymptomatic pneumococcal nasopharyngeal carriage to pneumonia and invasive disease. Dust exposure significantly reduced phagocyte-mediated bacterial killing, and exposure to high temperatures increased release of the key pneumococcal toxin pneumolysin through increased bacterial autolysis. Conclusion Our findings show that climatic factors can have a substantial influence on infectious disease patterns, altering density of pneumococcal nasopharyngeal carriage, reducing phagocytic killing, and resulting in increased inflammation and tissue damage and consequent invasiveness. Climatic surveillance should be used to forecast invasive bacterial disease epidemics, and simple control measures to reduce particulate inhalation might reduce the incidence of invasive bacterial disease in regions of the world exposed to high temperatures and increased airborne dust

BioTIME: A database of biodiversity time series for the Anthropocene.

MotivationThe BioTIME database contains raw data on species identities and abundances in ecological assemblages through time. These data enable users to calculate temporal trends in biodiversity within and amongst assemblages using a broad range of metrics. BioTIME is being developed as a community-led open-source database of biodiversity time series. Our goal is to accelerate and facilitate quantitative analysis of temporal patterns of biodiversity in the Anthropocene.Main types of variables includedThe database contains 8,777,413 species abundance records, from assemblages consistently sampled for a minimum of 2 years, which need not necessarily be consecutive. In addition, the database contains metadata relating to sampling methodology and contextual information about each record.Spatial location and grainBioTIME is a global database of 547,161 unique sampling locations spanning the marine, freshwater and terrestrial realms. Grain size varies across datasets from 0.0000000158 km2 (158 cm2) to 100 km2 (1,000,000,000,000 cm2).Time period and grainBioTIME records span from 1874 to 2016. The minimal temporal grain across all datasets in BioTIME is a year.Major taxa and level of measurementBioTIME includes data from 44,440 species across the plant and animal kingdoms, ranging from plants, plankton and terrestrial invertebrates to small and large vertebrates.Software format.csv and .SQL

The Resolved Stellar Population of the Post-Starburst Galaxy NGC 1569

We present WFPC2-HST photometry (in the F439W and F555W bands) of the resolved stars in NGC 1569. The derived color-magnitude diagram (CMD) contains 2800 objects down to the 26th mag, and is complete down to the 23rd. Adopting the literature distance modulus and reddening, the CMD samples stars more massive than 4 Mo, corresponding to a look-back time of 0.15 Gyr approximately. The data are compared to synthetic CMDs to derive the recent star formation history in NGC 1569. It is found that the observed field has experienced a global burst of star formation (SF) lasting at least 0.1 Gyr, and ended a few Myr ago. During the burst, the SF rate was approximately constant, and, if quiescent periods occurred, they lasted less than 10 Myr. The level of the SF rate was very high: 0.5 Mo/yr for a Salpeter IMF, in stars with mass ranging from 0.1 to 120 Mo. When scaled for the surveyed area, the SF rate is approximately 100 times larger than found in the most active dwarf irregulars in the Local Group. The data are consistent with a Salpeter IMF, or slightly steeper exponents. We discuss the implications of our results in the general context of the evolution of dwarf galaxies.Comment: 63 pages including 2 tables and 12 figures, to be published in The Astrophysical Journa