Supplementation of Cattle with Rock Phosphate and Urea Treated Straw to Improve Manure Quality and Crop Yields in the Sahel Zone of Senegal

Mineral deficiencies are a major constraint in improving animal production and crop yield in the Sahel zone (Cissé et al., 1996). Millet (Pennisetum glaucum) and groundnut (Arachis hypogaea,) are two major food and cash crops in this zone. The purpose of this study was to assess effects of supplementing grazing cattle with rock phosphate and nitrogen enriched diets on animal performances, and the effects of the application of their manure on crop yield in a pearl millet-groundnut rotational system located in N Senegal

Incidence and Risk Factors of First-Line HAART Discontinuation: Is it Worth Choosing Competing Risk or Standard Survival Approaches?

International audienceObjectives: To estimate the incidences of first-line HAART discontinuation (for intolerance, treatment failure or treatment simplification) and their risk factors by standard survival (1-KM, Cox model) or competing risk approach (CIF, Fine-Gray model) in HIV infected patients. Methods: We studied 1136 patients receiving first-line Highly Active Antiretroviral Therapies (HAART), aged over 18 years, from the Dat'AIDS cohort, Toulouse, France, between January 2000 and June 2008. Cumulative incidence was estimated with 1-KM and CIF estimators and risk factors with Cox and Fine-Gray models. Results: There were 265 discontinuations for intolerance, 136 simplifications, 101 treatment failure and 274 other reasons. One year incidences were 19.0% versus 16.8%, 8.0% versus 6.0%, 6.3% versus 4.8% and 20.0% versus 17.3%, with the estimators 1-KM and CIF, respectively. For intolerance, both models identified similar risk factors. For risk factors of simplification or treatment failure, results differed by the model. Conclusions: As expected, the 1-KM overestimates the incidence of treatment discontinuation. For early and frequent events such as intolerance, the Cox and the Fine-Gray models appear to give similar results. For late and rare events, potentially exposed to competing risk, results differed. The common or specific nature of a factor may also play a role

CRISPR System Acquisition and Evolution of an Obligate Intracellular Chlamydia-Related Bacterium.

Recently, a new Chlamydia-related organism, Protochlamydia naegleriophila KNic, was discovered within a Naegleria amoeba. To decipher the mechanisms at play in the modeling of genomes from the Protochlamydia genus, we sequenced the full genome of Pr. naegleriophila, which includes a 2,885,090 bp chromosome and a 145,285 bp megaplasmid. For the first time within the Chlamydiales order, we describe the presence of a clustered regularly interspaced short palindromic repeats (CRISPR) system, the immune system of bacteria, located on the chromosome. It is composed of a small CRISPR locus comprising eight repeats and associated cas-cse genes of the subtype I-E. A CRISPR locus is also present within Chlamydia sp. Diamant, another Pr. naegleriophila strain, suggesting that the CRISPR system was acquired by a common ancestor of Pr. naegleriophila, after its divergence from Pr. amoebophila. Both nucleotide bias and comparative genomics approaches identified probable horizontal gene acquisitions within two and four genomic islands in Pr. naegleriophila KNic and Diamant genomes, respectively. The plasmid encodes an F-type conjugative system highly similar to 1) that found in the Pam100G genomic island of Pr. amoebophila UWE25 chromosome, as well as on the plasmid of Rubidus massiliensis and 2) to the three genes remaining in the chromosome of Parachlamydia acanthamoebae strains. Therefore, this conjugative system was likely acquired on an ancestral plasmid before the divergence of Parachlamydiaceae Overall, this new complete Pr. naegleriophila genome sequence enables further investigation of the dynamic processes shaping the genomes of the family Parachlamydiaceae and the genus Protochlamydia

Profil Epidémiologique Des Risques Sanitaires Associés A La Fabrication Artisanale De Marmites En Aluminium A Saki Au Nigeria En 2016

L’étude a pour objectif d’analyser les risques sanitaires liés à la fabrication artisanale de marmites à partir de pièces de rechange en aluminium usagées récupérées et fondues à Saki au Nigéria. Il s’agit d’une étude transversale descriptive à visée analytique réalisée dans des ateliers de fonderie artisanale. Elle a duré 30 jours et a porté sur les fabricants. Tous ceux qui ont accepté participer librement à l’étude ont été enquêtés. Les données ont été collectées grâce à un questionnaire. Elles ont été traitées par le logiciel Épi Info 7.2. Le test x2 a été utilisé pour la comparaison de variables. Le taux de participation était 95,2%. Tous les enquêtés étaient de sexe masculin avec une moyenne d’âge 28,64 ± 13,29 ans. La tranche d’âge 8-18 ans étaient plus représentée. Les fabricants de marmites ne faisaient pas usage d’équipement de protection individuelle adapté. Ils souffraient de TMS et presque tous (96, 20%) ont été victimes d’accidents du travail. Étaient aussi évoqués la toux (57,59%), l’éternuement (54,43%), le larmoiement (55,06%), des macules cutanées (53,80%). Les fondeurs d’aluminium de Saki sont victimes de nombreuses pathologies et exposés à des risques sanitaires. The aim of the study isto analyze the health risks associated with homemade cooking pots from used aluminum spare parts recovered and melted in Saki, Nigeria. It was a descriptive, cross-sectional study for analytical purposes conducted in artisanal foundry workshops. It lasted 30 days and focused on the manufacturers. All who agreed to participate freely in the study were surveyed. The data was collected through a questionnaire. They were processed by the Epi Info 7.2 software. The x2 test was used for the comparison of variables. The participation rate was 95.2%. All were males of average age 28.64 ± 13.29 years. The age group 8-18 years were more represented. Nobody made use of suitable personal protective equipment. All had MSDs and almost all (96, 20%) were victims of work-related accidents. Cough (57.59%), sneezing (54.43%), watery eyes (55.06%) and skin macules (53.80%) were also mentioned. Many health risks are linked to the handcrafting of aluminum pots in Saki. &nbsp

Clinical and pathological kidney aspects of sickle cell anemia at Dakar: study of 11 cases of renal biopsies

Few studies are devoted to the practice of renal biopsy in sickle cell nephropathy; our objective was to determine the histological and evolutionary patterns of renal lesions in sickle cell patients who underwent renal biopsy in Dakar.Methods:This was a retrospective multicentric study (conducted from December 2009 to August 2011) on renal biopsies performed on sickle cell anaemic patients at the Nephrology Department of Teaching Hospital Aristide Le Dantec and the Albert Royer Childrens Hospital. The histological, therapeutic and evolutionary data were analysed.From the 292 total renal biopsies, 11 (3.80%) were performed on sickle cell patients (6SS, 1SBth + 4 AS) with a mean age of 23.1 [13-51 years]. Nephrotic syndrome was the indication of renal biopsy in all cases. Focal segmental glomerulosclerosis was the most frequent histological finding (five cases), followed by a combination of various specific lesions (hypertrophy of glomerular and peritubular capillaries), minimal glomerular lesions (three cases), membranoproliferative glomerulonephritis (two cases) and extra-membranous glomerulonephritis (one case). Complete remission after treatment was achieved in seven cases and one patient expired. Three patients did not continue with follow-up appointments.Conclusions:Renal biopsy is not very frequent in the course of sickle cell anaemia and in most cases it is performed because of nephrotic syndrome. The histological findings are diverse with a predominance of focal segmental glomerulosclerosis

Prevalence and associated risk factors of intestinal parasitic infections among children in pastoralist and agro-pastoralist communities in the Adadle woreda of the Somali Regional State of Ethiopia

BACKGROUND: Intestinal parasitic infections (IPIs) can cause illness, morbidity, and occasional mortality in children. Agro-pastoralist and pastoralist children in the Somali Regional State of Ethiopia (ESRS) are especially at risk for IPIs, as access to safe water, sanitation, and health services is lacking. Minimal data on the prevalence of IPIs and associated risk factors exists in this region. METHODOLOGY: We assessed the prevalence of IPIs and associated risk factors during the wet season from May-June 2021 in 366 children aged 2 to 5 years in four agro-pastoralist and four pastoralist kebeles (wards) in Adadle woreda (district) of the Shebelle zone, ESRS. Household information, anthropometric measurements, and stool samples were obtained from included children. Parasites were identified microscopically using Kato-Katz and direct smear methods. Risk factors were assessed using general estimating equation models accounting for clustering. PRINCIPAL FINDINGS: Overall prevalence of IPIs was 35%: 30.6% for single infections and 4.4% for poly-parasitic infections. Intestinal protozoan prevalence was 24.9%: 21.9% Giardia intestinalis, and 3.0% Entamoeba spp.. Intestinal helminth prevalence was 14.5%: 12.8% Ascaris lumbricoides, 1.4% hookworm (Ancylostoma duodenale /Necator americanus.), and 0.3% Hymenolepis nana. G. intestinalis infection was associated with drinking water sourced from the river (aOR 15.6, 95%CI 6.84, 35.4) and from collected rainwater (aOR 9.48, 95%CI 3.39, 26.5), with toilet sharing (aOR 2.93, 95%CI 1.36, 6.31) and with household ownership of cattle (1-5 cattle: aOR 1.65, 95%CI 1.13, 2.41; 6+ cattle: aOR 2.07, 95%CI 1.33, 3.21) and chickens (aOR 3.80, 95%CI 1.77, 8.17). A. lumbricoides infection was associated with children 36 to 47 months old (aOR 1.92, 95%CI 1.03, 3.58). CONCLUSIONS/SIGNIFICANCE: Improving access to safe water, sanitation, and hygiene services in Adadle and employing a One Health approach would likely improve the health of children living in (agro-) pastoralist communities in Adadle and the ESRS; however, further studies are required

Diarrhoea among children aged under five years and risk factors in informal settlements: a cross-sectional study in Cape Town, South Africa

Background: There is limited data on the association between diarrhoea among children aged under five years (U5D) and water use, sanitation, hygiene, and socio-economics factors in low-income communities. The study investigated U5D and the associated risk factors in the Zeekoe catchment in Cape Town, South Africa. Methods: A cross-sectional study was conducted in 707 households in six informal settlements (IS) two formal settlements (FS) (March-June 2017). Results: Most IS households used public taps (74.4%) and shared toilets (93.0%), while FS households used piped water on premises (89.6%) and private toilets (98.3%). IS respondents had higher average hand-washing scores than those of FS (0.04 vs