Estratégias para a vinculação das gestantes no pré-natal odontológico, em uma UBS de Curitiba

Orientador : Cristhiane Aparecida Mariot.Projeto Técnico (especialização) - Universidade Federal do Paraná, Setor de Ciências Sociais Aplicadas. Curso de Especialização em Gestão da SaúdeInclui referênciasResumo: Este trabalho é uma proposta de Projeto Técnico a ser realizada em uma Unidade Básica de Saúde do Município de Curitiba. Observa-se que nesta UBS um número reduzido de gestantes realiza a consulta odontológica durante o pré-natal, sendo que a realização destas consultas é importante para a orientação sobre os cuidados com a saúde bucal da mãe e do bebê, além do atendimento odontológico básico da gestante, evitando inflamações e infecções, as quais podem acarretar parto prematuro e bebê de baixo peso. Este projeto tem como objetivo a elaboração de propostas que visem a melhoria no acesso e no fluxo de atendimento das gestantes vinculadas nesta UBS, de uma forma que tenham um cuidado integral por toda a equipe de saúde. A metodologia utilizada para a comprovação da situação observada foi a busca por dados através de relatórios no sistema de saúde informatizado de Curitiba, o e-saúde. O resultado da análise destes dados indicou que apenas 9,2% das gestantes que foram vinculadas nesta UBS de fevereiro a outubro de 2013 realizaram pelo menos uma avaliação odontológica durante seu pré-natal. As propostas de melhoria incluíram o estabelecimento de um fluxo de assistência médico-odontológico no pré-natal, o fortalecimento da educação em saúde durante o pré-natal e a elaboração de um sistema de informação da gestante integrado com a odontologia

PESTICIDE RESIDUES EVALUATION IN BRAZILIAN BASIC DIET: RICE AND BEAN

Rice and bean constitute the basic diet in underdeveloped countries. In Brazil, the acquisition per capita of these aliments is around 182.9 g/day (beans) and 160.3 g/day (rice). In the present work were evaluated the pesticide residue contamination on these aliments, and possible risks for the consumer health. Pesticide residues were evaluated in 364 bean samples and 143 rice samples commercialized in Sao Paulo City (BRAZIL). A multi-residue method (DFG S19) was employed with gas and liquid chromatography identification and quantification. Dietary intake estimation was processed using the Acute Dietary Ingestion (ADI) based on national data regarding food consumption. Residues of pesticides that, according to the Brazilian legislation, are not allowed for this culture were detected respectively in 4.5% and 3.6% of the bean and rice samples. However, none of the samples had pesticide residues above the Maximum Residue Level (MRL). The European Union (EU) database showed that 7.3% of the bean sample and 85.7% of rice presented pesticide residues above the MRL. The toxicological parameter used for assessing the risk of contamination (ADI <100%) was not exceeded in none of the commodities. The results do not pose a hazard for the consumer’s health. Nevertheless, the presence of multiple residues indicates that continuous studies and monitoring of these commodities are required.

Ecotoxicological effects and bioaccumulation of BPA analogues and their mixture in the clam Ruditapes philippinarum

Bisphenol A is recognized as an endocrine disruptor that can affect several biological processes in marine species. Consequently, its use has been restricted and it has been replaced with other similar compounds named bisphenol A analogues (BPA analogues). BPA analogues are speculatively considered safer compounds than BPA and their usage is increasing with a consequent higher environmental release. In this study, specimens of the clam Ruditapes philippinarum were exposed to three main BPA analogues, namely BPAF, BPF, BPS and their mixture at an environmentally relevant concentration of 300 ng/L for 7 and 14 days. Effects on biomarkers indicative of cytotoxicity, oxidative stress and damage and neurotoxicity were evaluated. In addition, bioaccumulation of the compound tested was analysed in clam soft tissues. Results showed that BPA analogues at an environment concentration affected cellular parameters and antioxidant system causing also oxidative damage, suggesting that BPA analogues can be harmful compounds for clams

A LEGITIMIDADE ATIVA NO PROCESSO CIVIL COLETIVO BRASILEIRO

O presente artigo tem como objetivo a análise do instituto da legitimidade ativa dentro do sistema da tutela coletiva no Direito Processual Civil brasileiro. Trata-se de um dos temas mais controversos no processo coletivo, tendo em vista a inadequação da teoria utilizada pelo processo civil individual clássico, que não se amolda ao ideal de proteção coletiva previsto em outras leis, como a Lei da Ação Civil Pública e o Código de Defesa do Consumidor. Essa problemática não é resolvida pelo Novo Código de Processo Civil, já que este é voltado, majoritariamente, a tutelar a demanda individual

A multibiomarker approach in clams (Ruditapes philippinarum) for a toxicological evaluation of dredged sediments

The Lagoon of Venice is often dredged for channel maintenance. To avoid harmful consequences to the ecosystem, a proper disposal of bottom sediments requires a preliminary evaluation of its potential toxicity before excavation. Here we evaluated the effects of polluted sediments on clams (Ruditapes philippinarum) using a multibiomarker approach. Bivalves were exposed for 3 and 14 days to five sediment samples collected along a navigation canal between Venice historical centre and the industrial area of Porto Marghera. Immunological, antioxidant, detoxification, and neurotoxicity biomarkers were analysed in haemolymph, gill, and digestive gland. As a control, sediment collected far from pollution sources was used. Two experiments were performed to assess potential seasonal/gametogenic influence in clam sensitivity. A different response of clam biomarkers was observed during the two experiments and among sampling sites. Clams’ digestive gland resulted to be the most sensitive tissue analysed showing significant differences among sites in all biomarkers analysed. Greater differences were present due to seasonality rather than exposure. The concentrations of metals and organic pollutants increased from the city centre to the industrial area, highlighting the influence that industrial activities had on the lagoon ecosystem. However, bioaccumulation in clams did not follow the same clear pattern, suggesting low bioavailability of compounds due to relatively high organic matter content. Biomarkers modulation was mainly driven by metals, both present in sediments and bioaccumulated. In comparison, effects of organic pollutants on the biomarkers tested were negligible. Other sources of contamination not investigated (e.g. pesticides) were suggested by neurotoxicity biomarkers alteration

Contaminants from dredged sediments alter the transcriptome of Manila clam and induce shifts in microbiota composition

Background The reuse of dredged sediments in ports and lagoons is a big issue as it should not affect the quality and the equilibrium of ecosystems. In the lagoon of Venice, sediment management is of crucial importance as sediments are often utilized to built-up structures necessary to limit erosion. However, the impact of sediment reuse on organisms inhabiting this delicate area is poorly known. The Manila clam is a filter-feeding species of high economic and ecological value for the Venice lagoon experiencing a drastic decline in the last decades. In order to define the molecular mechanisms behind sediment toxicity, we exposed clams to sediments sampled from different sites within one of the Venice lagoon navigable canals close to the industrial area. Moreover, we investigated the impacts of dredged sediments on clam’s microbial communities. Results Concentrations of the trace elements and organic chemicals showed increasing concentrations from the city of Venice to sites close to the industrial area of Porto Marghera, where PCDD/Fs and PCBs concentrations were up to 120 times higher than the southern lagoon. While bioaccumulation of organic contaminants of industrial origin reflected sediments’ chemical concentrations, metal bioaccumulation was not consistent with metal concentrations measured in sediments probably due to the activation of ABC transporters. At the transcriptional level, we found a persistent activation of the mTORC1 signalling pathway, which is central in the coordination of cellular responses to chemical stress. Microbiota characterization showed the over-representation of potential opportunistic pathogens following exposure to the most contaminated sediments, leading to host immune response activation. Despite the limited acquisition of new microbial species from sediments, the latter play an important role in shaping Manila clam microbial communities. Conclusions Sediment management in the Venice lagoon will increase in the next years to maintain and create new canals as well as to allow the operation of the new mobile gates at the three Venice lagoon inlets. Our data reveal important transcriptional and microbial changes of Manila clams after exposure to sediments, therefore reuse of dredged sediments represents a potential risk for the conservation of this species and possibly for other organisms inhabiting the Venice lagoon

Clinical and molecular characterization of a cohort of patients with novel nucleotide alterations of the Dystrophin gene detected by direct sequencing

<p>Abstract</p> <p>Background</p> <p>Duchenne and Becker Muscular dystrophies (DMD/BMD) are allelic disorders caused by mutations in the dystrophin gene, which encodes a sarcolemmal protein responsible for muscle integrity. Deletions and duplications account for approximately 75% of mutations in DMD and 85% in BMD. The implementation of techniques allowing complete gene sequencing has focused attention on small point mutations and other mechanisms underlying complex rearrangements.</p> <p>Methods</p> <p>We selected 47 patients (41 families; 35 DMD, 6 BMD) without deletions and duplications in <it>DMD </it>gene (excluded by multiplex ligation-dependent probe amplification and multiplex polymerase chain reaction analysis). This cohort was investigated by systematic direct sequence analysis to study sequence variation. We focused our attention on rare mutational events which were further studied through transcript analysis.</p> <p>Results</p> <p>We identified 40 different nucleotide alterations in DMD gene and their clinical correlates; altogether, 16 mutations were novel. DMD probands carried 9 microinsertions/microdeletions, 19 nonsense mutations, and 7 splice-site mutations. BMD patients carried 2 nonsense mutations, 2 splice-site mutations, 1 missense substitution, and 1 single base insertion. The most frequent stop codon was TGA (n = 10 patients), followed by TAG (n = 7) and TAA (n = 4). We also analyzed the molecular mechanisms of five rare mutational events. They are two frame-shifting mutations in the <it>DMD </it>gene 3'end in BMD and three novel splicing defects: IVS42: c.6118-3C>A, which causes a leaky splice-site; c.9560A>G, which determines a cryptic splice-site activation and c.9564-426 T>G, which creates pseudoexon retention within IVS65.</p> <p>Conclusion</p> <p>The analysis of our patients' sample, carrying point mutations or complex rearrangements in <it>DMD </it>gene, contributes to the knowledge on phenotypic correlations in dystrophinopatic patients and can provide a better understanding of pre-mRNA maturation defects and dystrophin functional domains. These data can have a prognostic relevance and can be useful in directing new therapeutic approaches, which rely on a precise definition of the genetic defects as well as their molecular consequences.</p

A LEGITIMIDADE ATIVA NO PROCESSO CIVIL COLETIVO BRASILEIRO

O presente artigo tem como objetivo a análise do instituto da legitimidade ativa dentro do sistema da tutela coletiva no Direito Processual Civil brasileiro. Trata-se de um dos temas mais controversos no processo coletivo, tendo em vista a inadequação da teoria utilizada pelo processo civil individual clássico, que não se amolda ao ideal de proteção coletiva previsto em outras leis, como a Lei da Ação Civil Pública e o Código de Defesa do Consumidor. Essa problemática não é resolvida pelo Novo Código de Processo Civil, já que este é voltado, majoritariamente, a tutelar a demanda individual.</p

Arquivo universitário:implantação de políticas de gestão documental no gabinete do vice-reitor da UFSM

Este trabalho é resultado da pesquisa realizada no Gabinete do Vice-Reitor da Universidade Federal de Santa Maria – RS. Tem como finalidade apresentar as atividades de implantação de políticas de gestão documental, a fim de dar continuidade à implementação e estabelecimento de políticas adotadas pelo Departamento de Arquivo Geral. Assim, evidencia-se a relevância de seguir as políticas do Sistema de Arquivos da Universidade Federal de Santa Maria (SIARQ), e também de incluir as acadêmicas responsáveis pelo projeto nas práticas teórico-científicas, a fim de aliar a teoria adquirida no decorrer do curso de Arquivologia com a prática de uma realidade institucional. Dessa maneira a implementação de uma política de gestão documental passa a ser de extrema relevância dentro de uma instituição, contribuindo para a eficiência na agilidade e no acesso à informação

University archives: implementation of policies in document management office of the vice-rector of UFSM

Submitted by Jéssica Velho ([email protected]) on 2013-10-24T17:42:15Z No. of bitstreams: 1 Arquivo universitário implantação de políticas de gestão de documentos no gabinete do vice-reitor UFSM.pdf: 162806 bytes, checksum: c13ea8e809a35b693bc9d6e4ee51aa19 (MD5)Approved for entry into archive by Gabriela Silva da Rosa ([email protected]) on 2013-10-25T19:26:41Z (GMT) No. of bitstreams: 1 Arquivo universitário implantação de políticas de gestão de documentos no gabinete do vice-reitor UFSM.pdf: 162806 bytes, checksum: c13ea8e809a35b693bc9d6e4ee51aa19 (MD5)Made available in DSpace on 2013-10-25T19:26:41Z (GMT). No. of bitstreams: 1 Arquivo universitário implantação de políticas de gestão de documentos no gabinete do vice-reitor UFSM.pdf: 162806 bytes, checksum: c13ea8e809a35b693bc9d6e4ee51aa19 (MD5) Previous issue date: 2012Este trabalho é resultado da pesquisa realizada no Gabinete do Vice-Reitor da Universidade Federal de Santa Maria – RS. Tem como finalidade apresentar as atividades de implantação de políticas de gestão documental, a fim de dar continuidade à implementação e estabelecimento de políticas adotadas pelo Departamento de Arquivo Geral. Assim, evidencia-se a importância de seguir as políticas do Sistema de Arquivos da Universidade Federal de Santa Maria (SIARQ), e também de incluir as acadêmicas responsáveis pelo projeto nas práticas teórico-científicas, a fim de aliar a teoria adquirida no decorrer do curso de Arquivologia com a prática de uma realidade institucional. Dessa maneira Para alcançar este objetivo, primeiramente foi realizado um diagnóstico do acervo documental para subsidiar a proposta de ações arquivísticas. Esta proposta, elaborada com base na revisão de literatura realizada, foi aprovada. Entre junho e novembro de 2011 esta proposta foi implementada. Neste sentido, este trabalho descreve a implementação desta política de gestão documental, ressaltando a sua relevância dentro de uma instituição, contribuindo para a eficiência na agilidade e no acesso à informação.This work is the result of research conducted in the Office of the Vice Chancellor, Federal University of Santa Maria - RS. Its purpose is to present the activities of implementing document management policies in order to continue the implementation and establishment of policies adopted by the General Department of Archives. Thus, it becomes clear the importance of following the policies of the File System, Federal University of Santa Maria (SIARQ), and also include the academic responsible for the project in the theoretical and scientific practices in order to combine the theory acquired in the course of the Archival course of the practice of an institutional reality. Thus to achieve this goal, we first performed a diagnosis of documentary collection to support the proposed actions archival. This proposal, which is based on literature review, was approved. Between June and November 2011 this proposal was implemented. Thus, this paper describes the implementation of this policy document management, highlighting its relevance within an institution, contributing to efficiency and agility in accessing information