The Mechanics of Collusion

the construction industry is according to the bribe Payers index from transParency international the most corruPt industry, well ahead of the defense sector. Corruption and collusion are closely interrelated in the public sector, they nurture each other. However, collusion does not depend on cor- ruption, it can thrive by itself. A basic question is whether collusion is mostly a moral or an institutional issue. Using the title “mechan- ics of collusion” and proving the implied automatic reactions clearly strengthens the argument for an institutional view. There is no free- dom of choice in mechanics and accordingly there are no moral issues. The predominant antecedents of the construction industry (sealed-bid auctions, competitive environment, large contract volumes) are setting incentives for collusive cooperation. Such a behavior can even be morally defended. Since it is illegal (while extant) in most countries, it can not be encouraged. However, it is worthwhile to discuss changes to the procurement process that will set incentives to discourage collusion

Adoption of Collaborative Technology to Enhance Master Data Quality Across Municipal Administrations - Identifying Drivers and Barriers

In recent years, software for collaborative improvement of master data quality has been increasingly introduced into public administration. With the support of this collaborative software, master data is not only managed by one stakeholder such as an individual or department but rather cross-institutionally and across departments by groups of several stakeholders, e.g. several municipal authorities. In this paper, based on the Technology-Organization-Environment Framework, both driving forces as well as obstacles were identified, which influenced the adoption of such collaborative software. The results show, that positive factors concerning the adoption are perceived barriers, presence and importance of data standards as well as the successful implementation into the existing environment, whereas a complex IT-infrastructure impedes an introduction

Autaptic cultures of human induced neurons as a versatile platform for studying synaptic function and neuronal morphology

Recently developed technology to differentiate induced pluripotent stem cells (iPSCs) into human induced neurons (iNs) provides an exciting opportunity to study the function of human neurons. However, functional characterisations of iNs have been hampered by the reliance on mass culturing protocols which do not allow assessment of synaptic release characteristics and neuronal morphology at the individual cell level with quantitative precision. Here, we have developed for the first time a protocol to generate autaptic cultures of iPSC-derived iNs. We show that our method efficiently generates mature, autaptic iNs with robust spontaneous and action potential-driven synaptic transmission. The synaptic responses are sensitive to modulation by metabotropic receptor agonists as well as potentiation by acute phorbol ester application. Finally, we demonstrate loss of evoked and spontaneous release by Unc13A knockdown. This culture system provides a versatile platform allowing for quantitative and integrative assessment of morphophysiological and molecular parameters underlying human synaptic transmission

From hybridomas to a robust microalgal-based production platform: molecular design of a diatom secreting monoclonal antibodies directed against the Marburg virus nucleoprotein

Background: The ideal protein expression system should provide recombinant proteins in high quality and quantity involving low production costs only. However, especially for complex therapeutic proteins like monoclonal antibodies many challenges remain to meet this goal and up to now production of monoclonal antibodies is very costly and delicate. Particularly, emerging disease outbreaks like Ebola virus in Western Africa in 2014–2016 make it necessary to reevaluate existing production platforms and develop robust and cheap alternatives that are easy to handle. Results: In this study, we engineered the microalga Phaeodactylum tricornutum to produce monoclonal IgG antibodies against the nucleoprotein of Marburg virus, a close relative of Ebola virus causing severe hemorrhagic fever with high fatality rates in humans. Sequences for both chains of a mouse IgG antibody were retrieved from a murine hybridoma cell line and implemented in the microalgal system. Fully assembled antibodies were shown to be secreted by the alga and antibodies were proven to be functional in western blot, ELISA as well as IFA studies just like the original hybridoma produced IgG. Furthermore, synthetic variants with constant regions of a rabbit IgG and human IgG with optimized codon usage were produced and characterized. Conclusions: This study highlights the potential of microalgae as robust and low cost expression platform for monoclonal antibodies secreting IgG antibodies directly into the culture medium. Microalgae possess rapid growth rates, need basically only water, air and sunlight for cultivation and are very easy to handle

Trends of atmospheric water vapour in Switzerland from ground-based radiometry, FTIR and GNSS data

Vertically integrated water vapour (IWV) is expected to increase globally in a warming climate. To determine whether IWV increases as expected on a regional scale, we present IWV trends in Switzerland from ground-based remote sensing techniques and reanalysis models, considering data for the time period 1995 to 2018. We estimate IWV trends from a ground-based microwave radiometer in Bern, from a Fourier transform infrared (FTIR) spectrometer at Jungfraujoch, from reanalysis data (ERA5 and MERRA-2) and from Swiss ground-based Global Navigation Satellite System (GNSS) stations. Using a straightforward trend method, we account for jumps in the GNSS data, which are highly sensitive to instrumental changes. We found that IWV generally increased by 2 % per decade to 5 % per decade,with deviating trends at some GNSS stations. Trends were significantly positive at 17 % of all GNSS stations, which of-ten lie at higher altitudes (between 850 and 1650 m above sea level). Our results further show that IWV in Bern scales to air temperature as expected (except in winter), but the IWV–temperature relation based on reanalysis data in the whole of Switzerland is not clear everywhere. In addition to our positive IWV trends, we found that the radiometer in Bern agrees within 5 % with GNSS and reanalyses. At the Jungfraujoch high-altitude station, we found a mean difference of 0.26 mm (15 %) between the FTIR and coincident GNSS data, improving to 4 % after an antenna update in 2016. In general,we showed that ground-based GNSS data are highly valuable for climate monitoring, given that the data have been homogeneously reprocessed and that instrumental changes are accounted for. We found a response of IWV to rising temperature in Switzerland, which is relevant for projected changes in local cloud and precipitation processe

Strangeness production in heavy ion reactions at intermediate energies

Kaon production, in particular $K^+$ production in heavy ion collisions at intermediate energies is discussed. Main emphasis is put on the question if subthreshold $K^+$ production can serve as a suitable tool to test the high density phase of such reactions and to deliver information on the high density behavior of the nuclear equation of state. It is shown that the $K^+$ excitation function in heavy ($Au+Au$) over light ($C+C$) systems provides a robust observable which, by comparison to data, strongly favors a soft equation of state. A second question of interest is the existence of an in-medium kaon potential as predicted by effective chiral Lagrangiens. Here it is argued that transport calculations support this scenario with, in the meantime, a significant level of consistency.Comment: Invited talk at the International School of Nuclear Physics, ''Heavy Ion Reactions from Nuclear to Quark Matter'', Erice (It), Sep. 16 - 24, 2003 (typos corrected in v2

Outbreak of leptospirosis among triathlon participants in Germany, 2006

<p>Abstract</p> <p>Background</p> <p>In August 2006, a case of leptospirosis occurred in an athlete after a triathlon held around Heidelberg and in the Neckar river. In order to study a possible outbreak and to determine risk factors for infection an epidemiological investigation was performed.</p> <p>Methods</p> <p>Participants of the triathlon were contacted by e-mail and were asked to fill out a standardized questionnaire. In addition, they were asked to supply a serum sample for laboratory diagnosis of leptospirosis. A confirmed case patient was defined as a clinical case (i.e. fever and at least one additional symptom suggestive for leptospirosis) with at least two of the following tests positive: ELISA IgM, latex agglutination testing, or microscopic agglutination testing. Rainfall and temperature records were obtained.</p> <p>Results</p> <p>A total of 142 of 507 triathletes were contacted; among these, five confirmed leptospirosis cases were found. Open wounds were identified as the only significant risk factor for illness (p = 0.02). Heavy rains that preceded the swimming event likely increased leptospiral contamination of the Neckar River.</p> <p>Discussion</p> <p>This is the first outbreak of leptospirosis related to a competitive sports event in Germany. Among people with contact to freshwater, the risk of contracting leptospirosis should be considered by health care providers also in temperate countries, particularly in the summer after heavy rains.</p