Morphologically indistinguishable hybrid Carassius female with 156 chromosomes : A threat for the threatened crucian carp, C. carassius, L

The crucian carp Carassius carassius (Linnaeus, 1758), is native to many European freshwaters. Despite its wide distribution, the crucian carp is declining in both the number and sizes of populations across much of its range. Here we studied 30 individuals of a putative pure population from Helsinki, Finland. Despite clear external morphological features of C. carassius, an individual was of a higher ploidy level than the others. We therefore applied a set of molecular genetic (S7 nuclear and cytochrome b mitochondrial genes) and cytogenetic tools (sequential fluorescent 4’, 6-diamidino-2-phenylindole [DAPI], Chromomycin A3 [CMA3], C-banding and in situ hybridization [FISH] with both 5S and 28S ribosomal DNA probes) to determine its origin. While all examined characteristics of a diploid representative male (CCAHe2Fi) clearly corresponded to those of C. carassius, a triploid individual (CCAHe1Fi) was more complex. Phylogenetic analysis revealed that the nuclear genome of CCAHe1Fi contained three haploid sets: two C. gibelio and one C. carassius. However the mitochondrial DNA was that of C. gibelio, demonstrating its hybrid origin. The FISH revealed three strong (more intensive) 5S rDNA loci, confirming the triploid status, and an additional 24 weak (less intensive) signals were observed in the chromosome complement of CCAHe1Fi. On the other hand, only two strong and 16 weak 5S rDNA signals were visible on the chromosomes of the CCAHe2Fi male. 28S rDNA FISH revealed four strong signals in both CCAHe1Fi and CCAHe2Fi individuals. CMA3 staining revealed four to six CMA3-positive bands of CCAHe1Fi, while that of diploids contained only two to four. The fact that a polyploid hybrid Carassius female with a strong invasive potential may share morphological characters typical for endangered C. carassius highlights a need to combine genetic investigations of Carassius cryptic diversity with conservation measures of C. carassius in Europe.Peer reviewe

Lost sex: multilocus DNA evolution the hybrid fish complex C.taenia (Teleostei)

12 General summary "It is unwise to intrude the suggestion of parthenogenesis, even of a modified sort, into vertebrate literatures. The phenomenon is so at variance with what is known and beleived about vertebrates development that I am sure no vertebrate morphologists would admit for a moment that the natural development from egg to sexual maturity of an individual vertebrate without the direct inclusion of the male element is within the realm of probability", reacted the morphologist Howell (1933. Science 77: 389-390) sarcastically on the discovery of the first recognized asexual vertebrate, a live-bearing fish Poecilia formosa (Hubbs et Hubbs 1932. Science 30: 628-630). "The examples of parthenogenesis has been reported in almost all vertebrate groups," (Lampert 2008. Sexual Development 2: 290-301). The ongoing effort to explain the widespread occurrence of sexual reproduction among animals is often called the "queen of problems in evolutionary biology", and there is still no consensus regarding the explanation. Simultaneously, asexual reproduction exists among some animals. Here, the 'asexuality' refers to any reproductive process that does not involve sex (Mendelian meiosis is mising), except self-fertilization. One individuum produces a progeny genetically identical to its parent, except those sites..

Hybridogenetická reprodukce v komplexu Rana esculenta v horním odvodnění řeky Odry

Katedra zoologieDepartment of ZoologyFaculty of SciencePřírodovědecká fakult

All-male hybrids of a tetrapod Pelophylax esculentus share its origin and genetics of maintenance

Background Sexual parasites offer unique insights into the reproduction of unisexual and sexual populations. Because unisexuality is almost exclusively linked to the female sex, most studies addressed host-parasite dynamics in populations where sperm-dependent females dominate. Pelophylax water frogs from Central Europe include hybrids of both sexes, collectively named P. esculentus. They live syntopically with their parental species P. lessonae and/or P. ridibundus. Some hybrid lineages consist of all males providing a chance to understand the origin and perpetuation of a host-parasite (egg-dependent) system compared to sperm-dependent parthenogenesis. Methods We focused on P. ridibundus-P. esculentus populations where P. ridibundus of both sexes lives together with only diploid P. esculentus males. Based on 17 microsatellite markers and six allozyme loci, we analyzed (i) the variability of individual genomes, (ii) the reproductive mode(s) of all-male hybrids, and (iii) the genealogical relationships between the hybrid and parental genomes. Results Our microsatellite data revealed that P. esculentus males bear Mendelian-inherited ridibundus genomes while the lessonae genome represents a single clone. Our data indicate that this clone did not recently originate from adjacent P. lessonae populations, suggesting an older in situ or ex situ origin. Conclusions Our results confirm that also males can perpetuate over many generations as the unisexual lineage and successfully compete with P. ridibundus males for eggs provided by P. ridibundus females. Natural persistence of such sex-specific hybrid populations allows to studying the similarities and differences between male and female reproductive parasitism in many biological settings

Data from: Contrasting reproductive strategies of triploid hybrid males in vertebrate mating systems

The scarcity of parthenogenetic vertebrates is often attributed to their ‘inferior’ mode of clonal reproduction, which restricts them to self-reproduce their own genotype lineage and leaves little evolutionary potential with regard to speciation and evolution of sexual reproduction. Here, we show that for some taxa such uniformity does not hold. By using hybridogenetic water frogs (Pelophylax esculentus) as a model system, we demonstrate that triploid hybrid males from two geographic regions exhibit very different reproductive modes. With an integrative data set combining field studies, crossing experiments, flow cytometry and microsatellite analyses, we found that triploid hybrids from Central Europe are rare, occur in male sex only and form diploid gametes of a single clonal lineage. In contrast, triploid hybrids from North Western Europe are widespread, occur in both sexes and produce recombined haploid gametes. These differences translate into contrasting reproductive roles between regions. In Central Europe, triploid hybrid males sexually parasitize diploid hybrids and just perpetuate their own genotype – which is the usual pattern in parthenogens. In North Western Europe, on the other hand, the triploid males are gamete donors for diploid hybrids, thereby stabilizing the mixed 2n-3n hybrid populations. By demonstrating these contrasting roles in male reproduction, we draw attention to a new significant evolutionary potential for animals with non-sexual reproduction, namely reproductive plasticity

DNA microsatelitte allele data set

DNA microsatelitte data file for the 16 loci used in the study (RlCA5 and Res16 were not used for any of the analyses). (txt tabulated format

Morphological characteristics of a triploid, diploid representative and <i>C</i>. <i>carassius</i>.

<p>Morphological characteristics of a triploid, diploid representative and <i>C</i>. <i>carassius</i>.</p