3,628 research outputs found
Cell sorting in a Petri dish controlled by computer vision.
Fluorescence-activated cell sorting (FACS) applying flow
cytometry to separate cells on a molecular basis is a widespread
method. We demonstrate that both fluorescent and unlabeled live
cells in a Petri dish observed with a microscope can be
automatically recognized by computer vision and picked up by a
computer-controlled micropipette. This method can be routinely
applied as a FACS down to the single cell level with a very
high selectivity. Sorting resolution, i.e., the minimum distance
between two cells from which one could be selectively removed
was 50-70 micrometers. Survival rate with a low number of 3T3
mouse fibroblasts and NE-4C neuroectodermal mouse stem cells was
66 +/- 12% and 88 +/- 16%, respectively. Purity of sorted
cultures and rate of survival using NE-4C/NE-GFP-4C co-cultures
were 95 +/- 2% and 62 +/- 7%, respectively. Hydrodynamic
simulations confirmed the experimental sorting efficiency and a
cell damage risk similar to that of normal FACS
Improved genome editing in human cell lines using the CRISPR method
The Cas9/CRISPR system has become a popular choice for genome editing. In this system, binding of a single guide (sg) RNA to a cognate genomic sequence enables the Cas9 nuclease to induce a double-strand break at that locus. This break is next repaired by an error-prone mechanism, leading to mutation and gene disruption. In this study we describe a range of refinements of the method, including stable cell lines expressing Cas9, and a PCR based protocol for the generation of the sgRNA. We also describe a simple methodology that allows both elimination of Cas9 from cells after gene disruption and re-introduction of the disrupted gene. This advance enables easy assessment of the off target effects associated with gene disruption, as well as phenotype-based structure-function analysis. In our study, we used the Fan1 DNA repair gene as control in these experiments. Cas9/CRISPR-mediated Fan1 disruption occurred at frequencies of around 29%, and resulted in the anticipated spectrum of genotoxin hypersensitivity, which was rescued by re-introduction of Fan1
The effect of umbilical cord blood derived mesenchymal stem cells in monocrotaline-induced pulmonary artery hypertension rats
Pulmonary arterial hypertension (PAH) causes right ventricular failure due to a gradual increase in pulmonary vascular resistance. The purposes of this study were to confirm the engraftment of human umbilical cord blood-mesenchymal stem cells (hUCB-MSCs) placed in the correct place in the lung and research on changes of hemodynamics, pulmonary pathology, immunomodulation and several gene expressions in monocrotaline (MCT)induced PAH rat models after hUCB-MSCs transfusion. The rats were grouped as follows: the control (C) group; the M group (MCT 60 mg/kg); the U group (hUCB-MSCs transfusion). They received transfusions via the external jugular vein a week after MCT injection. The mean right ventricular pressure (RVP) was significantly reduced in the U group after the 2 week. The indicators of RV hypertrophy were significantly reduced in the U group at week 4. Reduced medial wall thickness in the pulmonary arteriole was noted in the U group at week 4. Reduced number of intra-acinar muscular pulmonary arteries was observed in the U group after 2 week. Protein expressions such as endothelin (ET)-1, endothelin receptor A (ERA), endothelial nitric oxide synthase (eNOS) and matrix metalloproteinase (MMP)-2 significantly decreased at week 4. The decreased levels of ERA, eNOS and MMP-2 immunoreactivity were noted by immnohistochemical staining. After hUCB-MSCs were administered, there were the improvement of RVH and mean RVP. Reductions in several protein expressions and immunomodulation were also detected. It is suggested that hUCB-MSCs may be a promising therapeutic option for PAH.1174Ysciescopu
The Student Movement Volume 105 Issue 7: Putting the Finishing Touches on Fall Semester
NEWS
Andrews Releases Spring 2021 Opening Plan, Amanda Cho
Creating Visions for the Future, Joelle Kim
Semester Reflections: Living During a Global Pandemic, Taylor Uphus
PULSE
The Best Drive-Through Meals, Masy Domecillo
Reviewing Leaving the Shadowland of Stress, Depression, and Anxiety , Jessica Rim
Study Tips: Re-Examining Routine, Wambui Karanja
HUMANS
Christmas vs. Thanksgiving, Interviewed by Abigail Lee
Interview with Dongchan Kim, AUSA Executive Vice President, Interviewd by Ben Lee
Studying Abroad in Argentina: An Interview with Lisiane Umuhire, Interviewed by TJ Hunter
ARTS & ENTERTAINMENT
Creative Spotlight: Ivan Rachath, Interviewed by Megan Napod
Elementary, Hannah Cruse
It\u27s Beginning to Sound A Lot Like Christmas!, Megan Napod
IDEAS
The Good, Kyara Samuels
The Power of Resource, Evin N. Musgrove
LAST WORD
Fallen Titans: Remembering Alex Trebek and Rabbi Jonathan Sacks, Daniel Selfhttps://digitalcommons.andrews.edu/sm-105/1008/thumbnail.jp
Scapular deformity in obstetric brachial plexus palsy: a new finding
While most obstetric brachial plexus palsy patients recover arm and hand function, the residual nerve weakness leads to muscle imbalances about the shoulder which may cause bony deformities. In this paper we describe abnormalities in the developing scapula and the glenohumeral joint. We introduce a classification for the deformity which we term Scapular Hypoplasia, Elevation and Rotation. Multiple anatomic parameters were measured in bilateral CT images and three-dimensional CT reconstruction of the shoulder girdle of 30 obstetric brachial plexus palsy patients (age range 10 months–10.6 years). The affected scapulae were found to be hypoplastic by an average of 14% while the ratio of the height to the width of the body of scapula (excluding acromion) were not significantly changed, the acromion was significantly elongated by an average of 19%. These parameters as well as subluxation of the humeral head (average 14%) and downward rotation in the scapular plane were found to correlate with the area of scapula visible over the clavicle. This finding provides a classification tool for diagnosis and objective evaluation of the bony deformity and its severity in obstetric brachial plexus palsy patients
Microwave amplification with nanomechanical resonators
Sensitive measurement of electrical signals is at the heart of modern science
and technology. According to quantum mechanics, any detector or amplifier is
required to add a certain amount of noise to the signal, equaling at best the
energy of quantum fluctuations. The quantum limit of added noise has nearly
been reached with superconducting devices which take advantage of
nonlinearities in Josephson junctions. Here, we introduce a new paradigm of
amplification of microwave signals with the help of a mechanical oscillator. By
relying on the radiation pressure force on a nanomechanical resonator, we
provide an experimental demonstration and an analytical description of how the
injection of microwaves induces coherent stimulated emission and signal
amplification. This scheme, based on two linear oscillators, has the advantage
of being conceptually and practically simpler than the Josephson junction
devices, and, at the same time, has a high potential to reach quantum limited
operation. With a measured signal amplification of 25 decibels and the addition
of 20 quanta of noise, we anticipate near quantum-limited mechanical microwave
amplification is feasible in various applications involving integrated
electrical circuits.Comment: Main text + supplementary information. 14 pages, 3 figures (main
text), 18 pages, 6 figures (supplementary information
The Student Movement Volume 105 Issue 2: Clubs Fare Well: Clubs Preserve Community During COVID
NEWS
Clubs, Clubs, Clubs Galore, Taylor Uphus
The Andreasen Center for Wellness Reopening, Amanda Cho
Danielle Pilgrim Delivers Inspiring Message, Terika Williams
PULSE
Planning University Club Events Amidst a Pandemic, Masy Domecillo
Research at the Science Complex During COVID-19, Jessica Rim
Socializing During a Pandemic: Freshmen Perspectives, Interviewed by Wambui Karanja
HUMANS
BSCF Club Spotlight, Interviewed by TJ Hunter
Distance Learning Student Interview, Sydney Saint-Jean
Meet Lamson Hall\u27s Newest Dean, Interviewed by Pearl Parker
Spiritual Leadership During COVID-19, Interviewed by Terika Williams
ARTS & ENTERTAINMENT
More Apples to Byte, Hannah Cruse
Playing Out the Pandemic: Our Orchestras Dealing with COVID, Megan Napod
Signal Boost: The Domino Effect, Pearl Parker
Virtual Game Night: A Virtual Reality, Joshua Deonarine
IDEAS
How Climate Change is Fueling the West Coast Fires, Lyle Goulbourne
Ideas That Heal Us: So You Think You\u27re Self-Aware, Evin N. Musgrove
THE LAST WORD
What am I Applying for & Who am I?, Daniel Selfhttps://digitalcommons.andrews.edu/sm-105/1002/thumbnail.jp
The Student Movement Volume 105 Issue 4: Students Destress in AUSA\u27s Nest
NEWS
AU Adelante Club Hosts Vespers, Joelle Kim
AUSA Hosts The Nest, Amanda Cho
The Gazebo Reopens with GetFood App, Taylor Uphus
PULSE
Cultural Hispanic Catchphrases, Wambui Karanja
Keep Calm and Breathing On (Yourself), Jessica Rim
Meet & Make: Reflections, Masy Domecillo
HUMANS
Event Planning With Malachi Regis, Interviewed by Fitz-Earl McKenzie II
Interview with Michael Nixon: Vice President for Diversity & Inclusion, Interviewed by Abigail Lee
Meet Professor Pedro Navia, Interviewed by Pearl Parker
Torian Hill, Interviewed by TJ Hunter
ARTS & ENTERTAINMENT
Hispanic Artist Feature: Felix Gillett, Megan Napod
Música para el Alma or Music for the Soul, Hannah Cruse
Signal Boost, Alannah Tjhatra
IDEAS
An Defense Against Burnout: Why Meaning Matters, Adoniah Simon
Remembering RBG: Part Two, Lyle Goulbourne
THE LAST WORD
How Does it Change Us?, Daniel Selfhttps://digitalcommons.andrews.edu/sm-105/1004/thumbnail.jp
Attention deficit hyperactivity symptoms predict problematic mobile phone use
Attention-deficit-hyperactivity disorder (ADHD) is the most commonly diagnosed childhood disorder characterised by inattention, hyperactivity/impulsivity, or both. Some of the key traits of ADHD have previously been linked to addictive and problematic behaviours. The aim of the present study was to examine the relationship between problematic mobile phone use, smartphone
addiction risk and ADHD symptoms in an adult population. A sample of 273 healthy adult volunteers completed the Adult
ADHD Self-Report Scale (ASRS), the Mobile Phone Problem Usage Scale (MPPUS), and the Smartphone Addiction Scale
(SAS). A significant positive correlation was found between the ASRS and both scales. More specifically, inattention symptoms
and age predicted smartphone addiction risk and problematic mobile phone use. Our results suggest that there is a positive
relationship between ADHD traits and problematic mobile phone use. In particular, younger adults with higher level of inattention symptoms could be at higher risk of developing smartphone addiction. The implication of our findings for theoretical
frameworks of problematic mobile phone use and clinical practice are discussed
Bisphenol A exposure in Mexico City and risk of prematurity: a pilot nested case control study
Abstract Background Presence of Bisphenol A (BPA) has been documented worldwide in a variety of human biological samples. There is growing evidence that low level BPA exposure may impact placental tissue development and thyroid function in humans. The aim of this present pilot study was to determine urinary concentrations of BPA during the last trimester of pregnancy among a small subset of women in Mexico City, Mexico and relate these concentrations to risk of delivering prematurely. Methods A nested case-control subset of 60 participants in the Early Life Exposure in Mexico to ENvironmental Toxicants (ELEMENT) study in Mexico City, Mexico were selected based on delivering less than or equal to 37 weeks of gestation and greater than 37 weeks of gestation. Third trimester archived spot urine samples were analyzed by online solid phase extraction coupled with high performance liquid chromatography isotope dilution tandem mass spectrometry. Results BPA was detected in 80.0% (N = 48) of the urine samples; total concentrations ranged from < 0.4 μg/L to 6.7 μg/L; uncorrected geometric mean was 1.52 μg/L. The adjusted odds ratio of delivering less than or equal to 37 weeks in relation to specific gravity adjusted third trimester BPA concentration was 1.91 (95%CI 0.93, 3.91, p-value = 0.08). When cases were further restricted to births occurring prior to the 37th week (n = 12), the odds ratio for specific-gravity adjusted BPA was larger and statistically significant (p < 0.05). Conclusions This is the first study to document measurable levels of BPA in the urine of a population of Mexican women. This study also provides preliminary evidence, based on a single spot urine sample collected during the third trimester, that pregnant women who delivered less than or equal to 37 weeks of gestation and prematurely (< 37 weeks) had higher urinary concentrations of BPA compared to women delivering after 37 weeks.http://deepblue.lib.umich.edu/bitstream/2027.42/78251/1/1476-069X-9-62.xmlhttp://deepblue.lib.umich.edu/bitstream/2027.42/78251/2/1476-069X-9-62.pdfPeer Reviewe
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