Nest food provisioning in the Red-capped Lark Calandrella cinerea does not vary with parental sex differences and time of day

The Red-capped Lark Calandrella cinerea inhabits some of the most highly threatened grassland ecosystems in Kenya. Although previous studies have been undertaken on feeding ecology of this species, a knowledge gap still exists in relation to its nest food provisioning behaviour. We studied the food provisioning behaviour of Red-capped Larks in open grassland habitat at Kedong Ranch in Naivasha, Kenya. Observations were completed on 18 active nests for a total of 163 observation hours between 07:00 and 18:00. Results confirmed that nestling diet comprised insect larvae (including caterpillars), grasshoppers, butterflies/moths, ants and beetles. Food provisioning rates for males and females combined, as well as independently, did not vary with the nestling age. In relation to specific prey items, provisioning rates of insect larvae and butterflies/moths during different hours of the day did not vary. However, there was a significant difference in provisioning rates of grasshoppers for the time periods. The findings provide an understanding of food requirements and feeding behaviour of the Red-capped Lark, and therefore are important for predicting how future changes in the availability of food resources could infl uence feeding, reproductive success, and possibly survival of the species.Keywords: Kedong Ranch, lark, grassland, nestling diet, insect

Towards sustainable partnerships in global health: the case of the CRONICAS Centre of Excellence in Chronic Diseases in Peru.

Human capital requires opportunities to develop and capacity to overcome challenges, together with an enabling environment that fosters critical and disruptive innovation. Exploring such features is necessary to establish the foundation of solid long-term partnerships. In this paper we describe the experience of the CRONICAS Centre of Excellence in Chronic Diseases, based at Universidad Peruana Cayetano Heredia in Lima, Peru, as a case study for fostering meaningful and sustainable partnerships for international collaborative research. The CRONICAS Centre of Excellence in Chronic Diseases was established in 2009 with the following Mission: "We support the development of young researchers and collaboration with national and international institutions. Our motivation is to improve population's health through high quality research." The Centre's identity is embedded in its core values - generosity, innovation, integrity, and quality- and its trajectory is a result of various interactions between multiple individuals, collaborators, teams, and institutions, which together with the challenges confronted, enables us to make an objective assessment of the partnership we would like to pursue, nurture and support. We do not intend to provide a single example of a successful partnership, but in contrast, to highlight what can be translated into opportunities to be faced by research groups based in low- and middle-income countries, and how these encounters can provide a strong platform for fruitful and sustainable partnerships. In defiant contexts, partnerships require to be nurtured and sustained. Acknowledging that all partnerships are not and should not be the same, we also need to learn from the evolution of such relationships, its key successes, hurdles and failures to contribute to the promotion of a culture of global solidarity where mutual goals, mutual gains, as well as mutual responsibilities are the norm. In so doing, we will all contribute to instil a new culture where expectations, roles and interactions among individuals and their teams are horizontal, the true nature of partnerships

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

American College of Rheumatology Provisional Criteria for Clinically Relevant Improvement in Children and Adolescents With Childhood-Onset Systemic Lupus Erythematosus

10.1002/acr.23834ARTHRITIS CARE & RESEARCH715579-59

2-D ZO CRS stack by considering an acquisition line with smooth topography

The land seismic data suffers from effects due to the near surface irregularities and the existence of topography. For obtaining a high resolution seismic image, these effects should be corrected by using seismic processing techniques, e.g. field and residual static corrections. The Common-Reflection-Surface (CRS) stack method is a new processing technique to simulate zero-offset (ZO) seismic sections from multi-coverage seismic data. It is based on a second-order hyperbolic paraxial traveltime approximation referred to a central normal ray. By considering a planar measurement surface, the CRS stacking operator is defined by means of three parameters, namely the emergence angle of the normal ray, the curvature of the normal incidence point (NIP) wave, and the curvature of the normal (N) wave. In this paper the 2-D ZO CRS stack method is modified in order to consider effects due to the smooth topography. By means of this new CRS formalism, we obtain a high resolution ZO seismic section, without applying static corrections. As by-products the 2-D ZO CRS stack method we estimate at each point of the ZO seismic section the three relevant parameters associated to the CRS stack process.Os dados sísmicos terrestres são afetados pela existência de irregularidades na superfície de medição, e.g. a topografia. Neste sentido, para obter uma imagem sísmica de alta resolução, faz-se necessário corrigir estas irregularidades usando técnicas de processamento sísmico, e.g. correições estáticas residuais e de campo. O método de empilhamento Superfície de Reflexão Comum, CRS ("Common-Reflection-Surface", em inglês) é uma nova técnica de processamento para simular seções sísmicas com afastamento-nulo, ZO ("Zero-Offset", em inglês) a partir de dados sísmicos de cobertura múltipla. Este método baseia-se na aproximação hiperbólica de tempos de trânsito paraxiais de segunda ordem referido ao raio (central) normal. O operador de empilhamento CRS para uma superfície de medição planar depende de três parâmetros, denominados o ângulo de emergência do raio normal, a curvatura da onda Ponto de Incidência Normal, NIP ("Normal Incidence Point", em inglês) e a curvatura da onda Normal, N. Neste artigo o método de empilhamento CRS ZO 2-D é modificado com a finalidade de considerar uma superfície de medição com topografia suave também dependente desses parâmetros. Com este novo formalismo CRS, obtemos uma seção sísmica ZO de alta resolução, sem aplicar as correições estáticas, onde em cada ponto desta seção são estimados os três parâmetros relevantes do processo de empilhamento CRS

A new bacteriophage P1-derived vector for the propagation of large human DNA fragments

We have designed a P1 vector (pCYPAC−1) for the introduction of recombinant DNA into E. coli using electroporation procedures. The new cloning system, P1−derived arteficial chromosomes (PACs), was used to establish an initial 15,000 clone library with an average insert size of 130−150 kilobase pairs (kb). No chimaerism has been observed in 34 clones, by fluorescence in situ hybridization. Similarly, no insert instability has been observed after extended culturing, for 20 clones. We conclude that the PAC cloning system will be useful in the mapping and detailed analysis of complex genomes