6 research outputs found
Degradación sono-fotocatalítica de orange II usando Fe₂O₃-TiO₂
Fe₂O₃-TiO₂ (1% de Fe) y muestras de TiO₂ puro (como referencia) fueron sintetizados por el método sol-gel. Los catalizadores Fe₂O₃-TiO₂ se prepararon para obtener un contenido de 1% de hierro. El TiO₂ sintetizado fue mezclado y molido con nanopartículas Fe₂O₃. Se determinó su estructura cristalográfica, parámetros de red y tamaño de partícula por difracción de rayos X. El área específica y diámetro de poro fueron determinados por el método BET (N₂ adsorción). Además, se realizaron estudios de degradación mediante reacciones fotocatalíticas y sono-fotocatalíticas, en la degradación del colorante Orange II (ultrasonido 250, 500 y 1000 kHz). Los materiales calcinados mostraron ser nanopartículas mesoporosas de TiO₂ en fase anatasa, con excelentes propiedades sonofotocatalíticas. La mejor actividad sono-fotocatalítica resultante fue mediante el uso de ultrasonido de 500 KHz. La longitud de onda de la fuente de luz no fue importante en las reacciones fotocatalíticas.Fe₂O₃-TiO₂ (1% Fe) samples and pure TiO₂ (as reference) were synthesized by the sol gel method. The Fe₂O₃-TiO₂ catalysts were prepared to obtain a content of 1% of iron. The synthesized TiO₂ was mixed and ground with Fe₂O₃ nanoparticles. Their crystallographic structure, lattice parameters and particle size were determined by X-ray diffraction. The specific area and pore diameter was studied by BET method (N2 adsorption). In addition, degradation studies using Orange II dye sono-photocatalysis were performed (ultrasound 250, 500 and 1000 kHz). The calcined materials showed TiO₂ nanoparticles in anatase phase and the mesoporous structures showed excellent sono-photocatalytic properties. Best sono-photocatalytic activity resulted with the use of ultrasound to 500 KHz. The wavelength of the light source was not important in photocatalytic reactions
A hotspot for posttranslational modifications on the androgen receptor dimer interface drives pathology and anti-androgen resistance
Mutations of the androgen receptor (AR) associated with prostate cancer and androgen insensitivity syndrome may profoundly influence its structure, protein interaction network, and binding to chromatin, resulting in altered transcription signatures and drug responses. Current structural information fails to explain the effect of pathological mutations on AR structure-function relationship. Here, we have thoroughly studied the effects of selected mutations that span the complete dimer interface of AR ligand-binding domain (AR-LBD) using x-ray crystallography in combination with in vitro, in silico, and cell-based assays. We show that these variants alter AR-dependent transcription and responses to anti-androgens by inducing a previously undescribed allosteric switch in the AR-LBD that increases exposure of a major methylation target, Arg761. We also corroborate the relevance of residues Arg761 and Tyr764 for AR dimerization and function. Together, our results reveal allosteric coupling of AR dimerization and posttranslational modifications as a disease mechanism with implications for precision medicine
Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant
TRAF1/C5 was among the first loci shown to confer risk for inflammatory arthritis in the absence of an associated coding variant, but its genetic mechanism remains undefined. Using Immunochip data from 3,939 patients with juvenile idiopathic arthritis (JIA) and 14,412 control individuals, we identified 132 plausible common non-coding variants, reduced serially by single-nucleotide polymorphism sequencing (SNP-seq), electrophoretic mobility shift, and luciferase studies to the single variant rs7034653 in the third intron of TRAF1. Genetically manipulated experimental cells and primary monocytes from genotyped donors establish that the risk G allele reduces binding of Fos-related antigen 2 (FRA2), encoded by FOSL2, resulting in reduced TRAF1 expression and enhanced tumor necrosis factor (TNF) production. Conditioning on this JIA variant eliminated attributable risk for rheumatoid arthritis, implicating a mechanism shared across the arthritis spectrum. These findings reveal that rs7034653, FRA2, and TRAF1 mediate a pathway through which a non-coding functional variant drives risk of inflammatory arthritis in children and adults
A hotspot for posttranslational modifications on the androgen receptor dimer interface drives pathology and anti-androgen resistance
Mutations of the androgen receptor (AR) associated with prostate cancer and androgen insensitivity syndrome may profoundly influence its structure, protein interaction network, and binding to chromatin, resulting in altered transcription signatures and drug responses. Current structural information fails to explain the effect of pathological mutations on AR structure-function relationship. Here, we have thoroughly studied the effects of selected mutations that span the complete dimer interface of AR ligand-binding domain (AR-LBD) using x-ray crystallography in combination with in vitro, in silico, and cell-based assays. We show that these variants alter AR-dependent transcription and responses to anti-androgens by inducing a previously undescribed allosteric switch in the AR-LBD that increases exposure of a major methylation target, Arg761. We also corroborate the relevance of residues Arg761 and Tyr764 for AR dimerization and function. Together, our results reveal allosteric coupling of AR dimerization and posttranslational modifications as a disease mechanism with implications for precision medicine
<i>Aedes aegypti</i> Strain Subjected to Long-Term Exposure to <i>Bacillus thuringiensis</i> svar. <i>israelensis</i> Larvicides Displays an Altered Transcriptional Response to Zika Virus Infection
Bacillus thuringiensis svar. israelensis (Bti) larvicides are effective in controlling Aedes aegypti; however, the effects of long-term exposure need to be properly evaluated. We established an Ae. aegypti strain that has been treated with Bti for 30 generations (RecBti) and is still susceptible to Bti, but females exhibited increased susceptibility to Zika virus (ZIKV). This study compared the RecBti strain to a reference strain regarding: first, the relative transcription of selected immune genes in ZIKV-challenged females (F30) with increased susceptibility detected in a previous study; then, the whole transcriptomic profile using unchallenged females (F35). Among the genes compared by RT-qPCR in the ZIKV-infected and uninfected females from RecBti (F30) and the reference strain, hop, domeless, relish 1, defensin A, cecropin D, and gambicin showed a trend of repression in RecBti infected females. The transcriptome of RecBti (F35) unchallenged females, compared with a reference strain by RNA-seq, showed a similar profile and only 59 differentially expressed genes were found among 9202 genes analyzed. Our dataset showed that the long-term Bti exposure of the RecBti strain was associated with an alteration of the expression of genes potentially involved in the response to ZIKV infection in challenged females, which is an important feature found under this condition
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History of tuberculosis disease is associated with genetic regulatory variation in Peruvians.
A quarter of humanity is estimated to have been exposed to Mycobacterium tuberculosis (Mtb) with a 5-10% risk of developing tuberculosis (TB) disease. Variability in responses to Mtb infection could be due to host or pathogen heterogeneity. Here, we focused on host genetic variation in a Peruvian population and its associations with gene regulation in monocyte-derived macrophages and dendritic cells (DCs). We recruited former household contacts of TB patients who previously progressed to TB (cases, n = 63) or did not progress to TB (controls, n = 63). Transcriptomic profiling of monocyte-derived DCs and macrophages measured the impact of genetic variants on gene expression by identifying expression quantitative trait loci (eQTL). We identified 330 and 257 eQTL genes in DCs and macrophages (False Discovery Rate (FDR) < 0.05), respectively. Four genes in DCs showed interaction between eQTL variants and TB progression status. The top eQTL interaction for a protein-coding gene was with FAH, the gene encoding fumarylacetoacetate hydrolase, which mediates the last step in mammalian tyrosine catabolism. FAH expression was associated with genetic regulatory variation in cases but not controls. Using public transcriptomic and epigenomic data of Mtb-infected monocyte-derived dendritic cells, we found that Mtb infection results in FAH downregulation and DNA methylation changes in the locus. Overall, this study demonstrates effects of genetic variation on gene expression levels that are dependent on history of infectious disease and highlights a candidate pathogenic mechanism through pathogen-response genes. Furthermore, our results point to tyrosine metabolism and related candidate TB progression pathways for further investigation