PO-278 The Mechanism Of LIL combined with AZD8055 On The Glutamine Addiction Of CT26 Cells

Objective The photobiomodulation of low-intensity lasers can activate many pathways and molecules, which involved in tumor growth, invasion, metastasis, and resistance to drugs or radiation therapy, but studies of low-intensity lasers on tumor cell addiction, have not been reported. Numerous studies have shown that changes in plasma and muscle glutamine levels can occur when the body undergoes prolonged exercise (>1 hour) or overtraining. AZD8055 is a dual inhibitor of mammalian rapamycin complex (mTORC)1/mTORC2 that inhibits phosphorylation of mTORC1 (p70S6K and 4E-BP1), mTORC2 (AKT) and downstream proteins. Based on the experimental model of glutamine addiction in laboratory, we chose the worst conditions to cause super-proliferation of CT26 cells and simulate the drug resistance of solid tumors. The purpose is to study tumor deterioration from the perspective of tumor cell function. Under the state, the effect of low-intensity laser combined with AZD8055 on the proliferation of glutamine addiction in CT26 cells is also the development of exercise intervention tumors, and the experimental basis is proposed from the perspective of nutrient metabolism. Methods This experiment used a pre-experimental glutamine addiction model to culture mouse colon cancer CT26 cells after horse serum shock, and used different intensity 640±15 nm low-intensity lasers from light-emitting diode arrays (red light at 640±15 nm from Light emitting diode array, RLED), 15 min/day；different concentrations of AZD8055 (0 ~ 100μM) on different proliferative CT26 cells at different times. Cell proliferation was detected by MTT assay; mRNA levels of proliferation-related genes were detected by RT-PCR; protein levels were detected by western blot. The data were analyzed by SPSS21.0 statistical software and self-similarity index analysis. Among them, the statistical significance level was set at 0.05, P<0.05 was different; P<0.01 was significant difference. In the self-similarity index analysis method, l>0.8 is a significant difference. Results 1.CT26 cells were incubated at Gln7.94 mM until the 8th day, and AZD8055 was added at different concentrations. Compared with no inhibitor , and at 6 h, the cells were significantly increased when the concentration of AZD8055 was 0.001 μM, 1 μM, 10 μM and 100 μM. (P<0.01); at 12h, the cells were significantly inhibition when the of AZD8055>0.5μM (P<0.01). 2.From the growth inhibition rate analysis, at 48 h and 72 h, the inhibition rate exceeded 50%,when the concentration of AZD8055 was 100 μM (61.01% and 87.46%), when< 0.1 μM,  the cells were proliferation occurred at 48 h and 72 h, and it is drug resistance. 3.When AZD8055 combined with low-intensity laser treatment of CT26 cells, CT26 cells had different degrees of proliferative effects at different time points: 12h, 24h, 48h and 72h. Especially at 48h, the light intensity of LIL2 to LIL8 had a significant proliferative effect on CT26 cells (l≥0.8). 4.Compared with the control group (0 hours without inhibitor), the CT26 cells were treatment with AZD8055 1μM , the mRNA level of the gene GLUT1 was significantly down-regulated in all time periods (P<0.01). When the LIL(2.17 mW/cm2) combined AZD8055 (1μM) significantly promoted the proliferation of CT26 cells at 24h and 48h. It is related to up-regulated of protein expression in all time points: at 6h and 12h, the pMEK protein and the pMEK/ MEK were significantly up-regulated(P<0.01). At 24h, the expression of GLUT1 protein was up-regulated(P<0.01), MEK protein, pERK protein and ERK/ pERK were significantly up-regulated (P<0.01).  At 48h, the GLUT1 protein, MEK protein , pERK protein and pERK/ERK were up-regulated (P<0.01). Conclusions 1.The minimum inhibitory concentration of AZD8055 on super-proliferative CT26 cells was 0.5 μM, and the super-proliferative state showed more obvious drug resistance. 2.When low-intensity lasers combined with AZD8055 treated CT26  super-proliferating cells, CT26 cells are not sensitive to AZD8055, while low-intensity lasers promote CT26 cells proliferation by up-regulating MEK/ERK signaling pathways

Concomitant percutaneous coronary intervention and transcatheter aortic valve replacement for aortic stenosis complicated with acute STEMI: a case report and literature review

Aortic stenosis (AS) complicated with acute ST-segment elevation myocardial infarction (STEMI) is a life-threatening emergency with high mortality. A 75-year-old male patient attended the emergency department of Wuhan Asia Heart Hospital in December 2021 with chest pain for 2 days and exacerbation for 1 h. The electrocardiogram (ECG) indicated atrial fibrillation with rapid ventricular response and ST-segment depression. Echocardiography showed severe AS and mild/moderate aortic insufficiency. The patient refused coronary angiography and further invasive procedures and then requested discharge, but he had recurrent chest pain on the third day. The ECG showed an extensive anterior wall STEMI. During preoperative preparation, he suffered from cardiogenic shock (CS). Concomitant percutaneous coronary intervention (PCI) and transcatheter aortic valve replacement (TAVR) was performed, but he died of CS and multiple organ failure 4 days after surgery. Patients with AS and STEMI might be susceptible to CS during perioperative period of concomitant PCI and TAVR, which requires proactive prevention

Trait Mindfulness Is Associated With the Self-Similarity of Heart Rate Variability

Previous studies have linked trait mindfulness with better self-regulation and adaptation. Heart rate variability (HRV) is a good physiological indicator of the capacity for self-regulation and adaptation. The present study explored the relationship between trait mindfulness and HRV from the viewpoint of crosstalking between different HRV parameter pairs, which would reflect the dynamic interactions between each pair of HRV parameters in different processes. We measured the trait mindfulness of seventy-four undergraduate students and recorded nine HRV parameters during the following four consecutive experimental phases: (1) calming phase, (2) mental arithmetic task phase, (3) recovery phase, and (4) mindfulness practice phase. The relationship between trait mindfulness and HRV was explored at the following three levels: (1) the absolute level, i.e., HRV parameters in four different states, (2) the difference-change level, i.e., differences in HRV parameters between different states, and (3) the crosstalking level, i.e., self-similarity of crosstalking HRV parameter pairs. The results supported the following hypothesis: trait mindfulness, as measured by the Mindful Attention Awareness Scale (MAAS), was significantly and positively correlated with the self-similarity of crosstalking HRV parameter pairs but was not significantly correlated with the HRV parameters at the difference-change and absolute levels. These findings indicate that as trait mindfulness increases, the ability to maintain ANS function homeostasis improves.HIGHLIGHTS-Trait mindfulness is associated with better self-regulation and adaptation.-Heart rate variability (HRV) is a good physiological indicator of the capacity for self-regulation and adaptation.-Trait mindfulness is significantly correlated with self-similarity of crosstalking HRV parameter pairs but not with the HRV parameters at the difference-change or absolute levels

原位表征技术揭示析氢电催化剂活性中心

文章简介课题组以导电碳布上生长的氢氧化镍纳米片为模板,通过与对苯二硫酚配体反应,成功合成出具有平面Ni（SR）4配位结构的镍—硫醇配位聚合物纳米片,并发现该材料在碱性析氢测试中,催化活性随测试时间逐渐加存在明显诱导期的现象。在保持20m A/cm2的电流密度条件下,析氢所需过电位逐渐降低,约10小时后该

Three-Dimensional Manganese Oxide@Carbon Networks as Free-Standing, High-Loading Cathodes for High-Performance Zinc-Ion Batteries

Zinc-ion batteries (ZIBs), which are inexpensive and environmentally friendly, have a lot of potential for use in grid-scale energy storage systems, but their use is constrained by the availability of suitable cathode materials. MnO2-based cathodes are emerging as a promising contenders, due to the great availability and safety, as well as the device's stable output voltage platform (1.5 V). Improving the slow kinetics of MnO2-based cathodes caused by low electrical conductivity and mass diffusion rate is a challenge for their future use in next-generation rapid charging devices. Herein, the aforementioned challenges are overcome by proposing a sodium-intercalated manganese oxide (NMO) with 3D varying thinness carbon nanotubes (VTCNTs) networks as appropriate free-standing, binder-free cathodes (NMO/VTCNTs) without any heat treatment. A network construction strategy based on CNTs of different diameters is proposed for the first time to provide high specific capacity while achieving high mass loading. The specific capacity of as-prepared cathodes is significantly increased. The resulting free-standing binder-free cathodes achieve excellent capacity (329 mAh g−1 after 120 cycles at 0.2 A g−1 and 225 mAh g−1 after 200 cycles at 1 A g−1) and long-term cycling stability (158 mAh g−1 at 2 A g−1 after 1000 cycles)

When It's Heavier: Interfacial and Solvation Chemistry of Isotopes in Aqueous Electrolytes for Zn-ion Batteries

The electrochemical effect of isotope (EEI) of water is introduced in the Zn-ion batteries (ZIBs) electrolyte to deal with the challenge of severe side reactions and massive gas production. Due to the low diffusion and strong coordination of ions in D2O, the possibility of side reactions is decreased, resulting in a broader electrochemically stable potential window, less pH change, and less zinc hydroxide sulfate (ZHS) generation during cycling. Moreover, we demonstrate that D2O eliminates the different ZHS phases generated by the change of bound water during cycling because of the consistently low local ion and molecule concentration, resulting in a stable interface between the electrode and electrolyte. The full cells with D2O-based electrolyte demonstrated more stable cycling performance which displayed ∼100 % reversible efficiencies after 1,000 cycles with a wide voltage window of 0.8–2.0 V and 3,000 cycles with a normal voltage window of 0.8–1.9 V at a current density of 2 A g−1

Antigen-Clustered Nanovaccine Achieves Long-Term Tumor Remission by Promoting B/CD 4 T Cell Crosstalk

Current cancer vaccines using T cell epitopes activate antitumor T cell immunity through dendritic cell/macrophage-mediated antigen presentation, but they lack the ability to promote B/CD4 T cell crosstalk, limiting their anticancer efficacy. We developed antigen-clustered nanovaccine (ACNVax) to achieve long-term tumor remission by promoting B/CD4 T cell crosstalk. The topographic features of ACNVax were achieved using an iron nanoparticle core attached with an optimal number of gold nanoparticles, where the clusters of HER2 B/CD4 T cell epitopes were conjugated on the gold surface with an optimal intercluster distance of 5-10 nm. ACNVax effectively trafficked to lymph nodes and cross-linked with BCR, which are essential for stimulating B cell antigen presentation-mediated B/CD4 T cell crosstalk

Evaluating the impact of groundwater on cotton growth and root zone water balance using Hydrus-1D coupled with a crop growth model

Groundwater is an important factor that needs to be considered when evaluating the water balance of the soil-plant-atmosphere system and the sustainable development of arid oases. However, the impact of shallow groundwater on the root zone water balance and cotton growth is not fully understood. In this study, we have first analyzed the influence of the groundwater table depth on the seasonal maximum leaf area index of cotton, the average seasonal water stress, cotton yield, actual transpiration, actual evaporation, and capillary rise using experimental data collected at the Aksu water balance station, in Xinjiang, northwest of China and the Hydrus-1D variably-saturated soil water flow model coupled with a simplified crop growth model from SWAT. The coupled model has been first calibrated and validated using field observations of soil water content, leaf area index, cotton height, the above ground biomass, and cotton yield comparisons between measured and modeled variables have shown a reasonable agreement for all variables. Additionally, with a validated model, we have carried out numerical experiments from which we have concluded that groundwater is a major water resource for cotton growth in this region. The capillary rise from groundwater contributes almost 23% of crop transpiration when the average groundwater depth is 1.84. m, which is the most suitable groundwater depth for this experimental site. We have concluded that cotton growth and various components of the soil water balance are highly sensitive to the groundwater table level. Different positions of the groundwater table showed both positive and negative effects on cotton growth. Likewise, cotton growth has a significant impact on the capillary rise from groundwater. As a result, groundwater is a crucial factor that needs to be considered when evaluating agricultural land management in this arid region. The updated Hydrus-1D model developed in this study provides a powerful modeling tool for evaluating the effects of the groundwater table on local land management

Electrostatic Self-Assembling Formation of Pd Superlattice Nanowires from Surfactant-Free Ultrathin Pd Nanosheets

A facile method has been developed for face-to-face assembly of two-dimensional surfactant-free Pd nanosheets into one-dimensional Pd superlattice nanowires. The length of the Pd nanowires can be well controlled by introducing cations of different concentration and charge density. Our studies reveal that cations with higher charge density have stronger charge-screening ability, and their introduction leads to more positive zeta-potential and decreased electrostatic repulsion between negatively charged Pd nanosheets. Moreover, their surfactant-free feature is of great importance in assembling the Pd nanosheets into superlattice nanowires. While the cations are important for the assembly of Pd nanosheets, the use of poly(vinylpyrrolidone) is necessary to enhance the stability of the assembled superlattice nanowires. The as-assembled segmented Pd nanowires display tunable surface plasmon resonance features and excellent hydrogen-sensing properties.MOST of China 2011CB932403 2014CB932004 NSFC 21131005 21333008 21420102001 NFFTBS J131002