Computer-Aided Value-Assessment Model: Review for Bilingual Teaching Courses Quantitative Analysis

AbstractIn order to review the effect on the bilingual teaching courses aided by computer, the comprehensive evaluation on the bilingual teaching course and research results of the bilingual teaching is required in the university. A full range system for bilingual teaching course performance assessment that is a novel quantized method is researched in this paper. Furthermore, the comprehensive evaluation processing and evaluation model have been accomplished. The application results for bilingual teaching course performance review assessment which is developed as a computer management system demonstrated its high operability and achieved accurate can be convenient for the same requirements

ENU-Induced Mutagenesis in Grass Carp (Ctenopharyngodon idellus) by Treating Mature Sperm

N-ethyl-N-nitrosourea (ENU) mutagenesis is a useful approach for genetic improvement of plants, as well as for inducing functional mutants in animal models including mice and zebrafish. In the present study, mature sperm of grass carp (Ctenopharyngodon idellus) were treated with a range of ENU concentrations for 45 min, and then wild-type eggs were fertilized. The results indicated that the proportion of embryos with morphological abnormalities at segmentation stage or dead fry at hatching stage increased with increasing ENU dose up to 10 mM. Choosing a dose that was mutagenic, but provided adequate numbers of viable fry, an F1 population was generated from 1 mM ENU-treated sperm for screening purposes. The ENU-treated F1 population showed large variations in growth during the first year. A few bigger mutants with morphologically normal were generated, as compared to the controls. Analysis of DNA from 15 F1 ENU-treated individuals for mutations in partial coding regions of igf-2a, igf-2b, mstn-1, mstn-2, fst-1and fst-2 loci revealed that most ENU-treated point mutations were GC to AT or AT to GC substitution, which led to nonsense, nonsynonymous and synonymous mutations. The average mutation rate at the examined loci was 0.41%. These results indicate that ENU treatment of mature sperm can efficiently induce point mutations in grass carp, which is a potentially useful approach for genetic improvement of these fish

Preparation of Tradescantia pallida-mediated zinc oxide nanoparticles and their activity against cervical cancer cell lines

Purpose: To synthesize zinc oxide nanoparticles (ZnO NPs) using Tradescantia pallida. (Commelinaceae) and determine their fluorescent and cytotoxic properties.Methods: ZnO NPs were synthesized according to a simple protocol using T. pallida aqueous leaf extract (TPALE). Scanning electron microscopy (SEM) and  transmission electron microscopy (TEM) were used to analyze the morphology of the ZnO NPs. X-ray diffraction (XRD) and Fourier transforminfrared spectroscopy (FTIR) measurements were performed to determine their crystalline nature and functional groups, respectively. Fluorescence spectroscopy was used to assess the  photoluminescence properties of ZnO NPs. Upon confirmation of ZnO NP synthesis, cytotoxicity tests were carried out against HeLa cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.Results: The agglomerated ZnO NPs were rod-shaped and had a mean particle size of 25 ± 2 nm. Further, they exhibited good photoluminescence with correlation to ZnO crystals. MTT assay results indicated significant cytotoxicity against HeLa cervical cancer cell line.Conclusion: A simple approach for ZnO NP synthesis based on TPALE has been developed successfully. The synthesized ZnO NPs demonstrate good luminescence properties and cytotoxicity against cervical cancer line.Keywords: Commelinaceae, Cytotoxicity, Photoluminescence, Setcreasea pallida, Setcreasea purpurea, Tradescantia pallida, ZnO nanoparticle

An efficient method for decellularization of the rat liver

Background/PurposeUsing gradient ionic detergent, we optimized the preparation procedure for the decellularized liver biologic scaffold, and analyzed its immunogenicity and biocompatibility.MethodsEDTA, hypotonic alkaline solution, Triton X-100, and gradient sodium dodecyl sulfate (1%, 0.5%, and 0.1%, respectively) were prepared for continuous perfusion through the hepatic vascular system. The decellularization of the liver tissue was performed with the optimized reagent buffer and washing protocol. In addition, the preservation of the original extracellular matrix was observed. To analyze its biocompatibility, the scaffold was embedded in a heterologous animal and the inflammation features, including the surrounding cell infiltration and changes of the scaffold architecture, were detected. The cell-attachment ability was also validated by the perfusion culture of HepG2 cells with the scaffold.ResultsBy using gradient ionic detergent, we completed the decellularization process in approximately 5 h, which was shorter than >10 hours in previous experiments (p<0.001). The extracellular matrix was kept relatively intact, with no obvious inflammatory cellular infiltration or structural damage in the grafted tissue. The engraftment efficiencies of HepG2 were 86±5% (n=8). The levels of albumin and urea synthesis were significantly superior to the ones in traditional two-dimensional culture.ConclusionThe current new method can be used efficiently for the decellularization of the liver biologic scaffold with satisfying biocomparability for application both in vivo and in vitro

Early Blockade of TLRs MyD88-Dependent Pathway May Reduce Secondary Spinal Cord Injury in the Rats

To determine the role of toll-like receptors (TLRs) myeloid differentiation factor 88 (MyD88) dependent pathway in the spinal cord secondary injury, compression injury was made at T8 segment of the spinal cord in adult male Sprague-Dawley rats. Shown by RT-PCR, TLR4 mRNA in the spinal cord was quickly elevated after compression injury. Intramedullary injection of MyD88 inhibitory peptide (MIP) resulted in significant improvement in locomotor function recovery at various time points after surgery. Meanwhile, injury area, p38 phosphorylation, and proinflammation cytokines in the injured spinal cord were significantly reduced in MIP-treated animals, compared with control peptide (CP) group. These data suggest that TLRs MyD88-dependent pathway may play an important role in the development of secondary spinal cord injury, and inhibition of this pathway at early time after primary injury could effectively protect cells from inflammation and apoptosis and therefore improve the functional recovery

PL - 038 Habitual swimming exercise induced partial resistance to rat Alzheimer's disease

Objective &nbsp;In MSSE, we&nbsp;have divided male 2.5-month-old Sprague-Dawley rats into the following 4 groups: control (C), habitual&nbsp;swimming (SW), Alzheimer’s disease (AD) induction without swimming (AD), and habitual swimming and then AD induction (SA), and found the perfect resistance of habitual swimming to AD induction by using the P value statistics of the 5 behavior parameters of rats and the 23 physiological and biochemical parameters of their hippocampus. The topological difference&nbsp; of four groups were further calculated in this paper by using quantitative difference (QD) and self-similar approach. Methods 1. The logarithm to base golden section τ&nbsp;(lt) is called golden logarithm. It was found that σ=ltσ&nbsp;≈&nbsp;0.710439287156503. 2. For a process from x1&nbsp;to x2, lx(1,2)=lt(x2/x1) and its absolute vale are called the process logarithm and its QD, QDx(1,2). There are&nbsp;QD threshold values (αx,βx,γx) of function x which can be calculated in terms of&nbsp;σ. The function x&nbsp;is kept to be constant&nbsp;if QDx(1,2) &lt; αx.&nbsp;A function in/far from its function-specific homeostasis&nbsp;is called a normal/dysfunctional function. A normal function can resist a disturbance under its threshold so that&nbsp;QDx(1,2) &lt; βx. A dysfunctional function is defined as the QD is significant if βx&nbsp;≦QDx(1,2) &lt; γx and extraordinarily significant if QDx(1,2) ≧&nbsp;γx.&nbsp;3. Self-similarity was&nbsp;studied&nbsp;in&nbsp;the fractal literature: a pattern is self-similar if it does not vary with spatial or temporal scale. First-order self-similarity condition leads to the power law between two data&nbsp;sets A = {xi}&nbsp;and B = {yi};&nbsp;yi&nbsp;= ai&nbsp;xi&nbsp;if the QDi&nbsp;of ai and the average of {ai} is smaller than βmin=min{βi} and the average QD of {QDi}&nbsp;is smaller than αmin=min{αi}. 4. The σ&nbsp;algorithm for integrative biology was established based on high-order self-similarity. Those parameters that contribute to&nbsp;the topological difference&nbsp;were the biomarkers. Results The 28 dimension data set consisted of all the 28 parameters. The first-order self-similarity held true for the 28 dimension data sets between groups C and SW.&nbsp;The topological algorithm of other groups suggested three AD biomarkers, protein&nbsp;carbonyl, granules density of presynaptic synaptophysin in the hippocampal CA1 and malondialdehyde intensity. The first two biomarkers were completely reversed by exercise pretreatment, but the third biomarker was partially reversed. Conclusions &nbsp;Exercise pretraining exerts partial&nbsp;benefits on AD that support its use as a promising new therapeutic option for prevention of neurodegeneration in the elderly and/or AD population.&nbsp

Whole-Genome Resequencing of Worldwide Wild and Domestic Sheep Elucidates Genetic Diversity, Introgression, and Agronomically Important Loci

Domestic sheep and their wild relatives harbor substantial genetic variants that can form the backbone of molecular breeding, but their genome landscapes remain understudied. Here, we present a comprehensive genome resource for wild ovine species, landraces and improved breeds of domestic sheep, comprising high-coverage (similar to 16.10x) whole genomes of 810 samples from 7 wild species and 158 diverse domestic populations. We detected, in total, similar to 121.2 million single nucleotide polymorphisms, similar to 61 million of which are novel. Some display significant (P < 0.001) differences in frequency between wild and domestic species, or are private to continent-wide or individual sheep populations. Retained or introgressed wild gene variants in domestic populations have contributed to local adaptation, such as the variation in the HBB associated with plateau adaptation. We identified novel and previously reported targets of selection on morphological and agronomic traits such as stature, horn, tail configuration, and wool fineness. We explored the genetic basis of wool fineness and unveiled a novel mutation (chr25: T7,068,586C) in the 3 '-UTR of IRF2BP2 as plausible causal variant for fleece fiber diameter. We reconstructed prehistorical migrations from the Near Eastern domestication center to South-and-Southeast Asia and found two main waves of migrations across the Eurasian Steppe and the Iranian Plateau in the Early and Late Bronze Ages. Our findings refine our understanding of genome variation as shaped by continental migrations, introgression, adaptation, and selection of sheep