An investigation on the mechanics of nanometric cutting and the development of its test-bed

The mechanics of machining at a very small depth of cut (100 nm or less) is not well understood. The chip formation physics, cutting forces generation, resulting temperatures and the size effects significantly affect the efficiency of the process and the surface quality of the workpiece. In this paper, the cutting mechanics at nanometric scale are investigated in comparison with conventional cutting principles. Molecular Dynamics (MD) is used to model and simulate the nanometric cutting processes. The models and simulated results are evaluated and validated by the cutting trials on an atomic force microscope (AFM). Furthermore, the conceptual design of a bench-type ultraprecision machine tool is presented and the machine aims to be a facility for nanometric cutting of threedimensional MEMS devices. The paper concludes with a discussion on the potential and applications of nanometric cutting techniques/equipment for the predictabilty, producibility and productivity of manufacturing at the nanoscale

An investigation on the mechanics of nanometric cutting and the development of its test-bed

The mechanics of machining at a very small depth of cut (100 nm or less) is not well understood. The chip formation physics, cutting forces generation, resulting temperatures and the size effects significantly affect the efficiency of the process and the surface quality of the workpiece. In this paper, the cutting mechanics at nanometric scale are investigated in comparison with conventional cutting principles. Molecular Dynamics (MD) is used to model and simulate the nanometric cutting processes. The models and simulated results are evaluated and validated by the cutting trials on an atomic force microscope (AFM). Furthermore, the conceptual design of a bench-type ultraprecision machine tool is presented and the machine aims to be a facility for nanometric cutting of threedimensional MEMS devices. The paper concludes with a discussion on the potential and applications of nanometric cutting techniques/equipment for the predictabilty, producibility and productivity of manufacturing at the nanoscale

Estimation and characterization of nonlinear behavior of nonlinear spatio-temporal systems

This Conference is organized by the Department of Structural and Geotechnical Engineering of the Sapienza University of Rome.Author name used in this publication: Jing, XingjianAuthor name used in this publication: Cheng, LiRefereed conference paper2011-2012 > Academic research: refereed > Refereed conference paperVersion of RecordPublishe

Comparison of hemolytic activity of the intermediate subunit of Entamoeba histolytica and Entamoeba dispar lectins

Galactose and N-acetyl-D-galactosamine-inhibitable lectin of Entamoeba histolytica has roles in pathogenicity and induction of protective immunity in rodent models of amoebiasis. Recently, the intermediate subunit of the lectin, Igl1, of E. histolytica has been shown to have hemolytic activity. However, the corresponding lectin is also expressed in a non-virulent species, Entamoeba dispar, and another subunit, Igl2, is expressed in the protozoa. Therefore, in this study, we compared the activities of Igl1 and Igl2 subunits from E. histolytica and E. dispar using various regions of recombinant Igl proteins expressed in Escherichia coli. The recombinant E. dispar Igl proteins had comparable hemolytic activities with those of E. histolytica Igl proteins. Furthermore, Igl1 gene-silenced E. histolytica trophozoites showed less hemolytic activity compared with vector-transfected trophozoites, indicating that the expression level of Igl1 protein influences the activity. These results suggest that the lower hemolytic activity in E. dispar compared with E. histolytica reflects the lower expression level of Igl1 in the E. dispar parasite

Short hairpin RNA-mediated knockdown of protein expression in Entamoeba histolytica

<p>Abstract</p> <p>Background</p> <p><it>Entamoeba histolytica </it>is an intestinal protozoan parasite of humans. The genome has been sequenced, but the study of individual gene products has been hampered by the lack of the ability to generate gene knockouts. We chose to test the use of RNA interference to knock down gene expression in <it>Entamoeba histolytica</it>.</p> <p>Results</p> <p>An episomal vector-based system, using the <it>E. histolytica </it>U6 promoter to drive expression of 29-basepair short hairpin RNAs, was developed to target protein-encoding genes in <it>E. histolytica</it>. The short hairpin RNAs successfully knocked down protein levels of all three unrelated genes tested with this system: Igl, the intermediate subunit of the galactose- and N-acetyl-D-galactosamine-inhibitable lectin; the transcription factor URE3-BP; and the membrane binding protein EhC2A. Igl levels were reduced by 72%, URE3-BP by 89%, and EhC2A by 97%.</p> <p>Conclusion</p> <p>Use of the U6 promoter to drive expression of 29-basepair short hairpin RNAs is effective at knocking down protein expression for unrelated genes in <it>Entamoeba histolytica</it>, providing a useful tool for the study of this parasite.</p

Observation of CR Anisotropy with ARGO-YBJ

The measurement of the anisotropies of cosmic ray arrival direction provides important informations on the propagation mechanisms and on the identification of their sources. In this paper we report the observation of anisotropy regions at different angular scales. In particular, the observation of a possible anisotropy on scales between $\sim$ 10 $^{\circ}$ and $\sim$ 30 $^{\circ}$ suggests the presence of unknown features of the magnetic fields the charged cosmic rays propagate through, as well as potential contributions of nearby sources to the total flux of cosmic rays. Evidence of new weaker few-degree excesses throughout the sky region $195^{\circ}\leq$ R.A. $\leq 315^{\circ}$ is reported for the first time.Comment: Talk given at 12th TAUP Conference 2011, 5-9 September 2011, Munich, German

Construction and demolition waste - a shift toward Lean Construction and Building Information Model

Waste in the construction industry is a devastating dilemma, especially that construction and demolition activities are considered as the highest waste generator globally. Countries have developed regulations: policy-makers and professional associations have provided norms and policies to manage C&D waste. Previous studies, however, have revealed insufficiencies in the current regulations and norms in incentivizing the industry practices toward waste prevention, since its culture is characterized by the gap in technological use, insufficient knowledge, poor planning, and poor information flow. This research provides a literature review on the current research findings and trends in managing C&D waste. Then based on design theory and theory of production, an exploratory research consisting of BIM and Lean construction concepts is provided. Lean can maximize the value of construction by addressing waste within portfolios, projects, and operations; BIM offers an enhanced collaborative platform with improved design practice and information management throughout buildings’ life cycle. The proposed conceptual framework enables economic, environmental, and social benefits to allow practitioners collaborate, analyze, and minimize construction waste throughout buildings’ life cycle.(undefined