Data collection and transmission for leisure time boats : based on Arduino WSNs and LTE

There has been an astonishing research development in the field of wireless sensor networks (WSNs) in the last decade. A large number of low power capacity devices have been implemented in different vehicles, where sensor nodes act as a team to monitor the environment and forecast the potential defects. In this thesis, we aim to design a data collection system using a WSN on a leisure boat in order to monitor and maintain the boat after sale. The designed system aims to collect data from different sensors on board using WSNs and transmits the collected data to a remote server through cellular network. For the WSNs part, we select a low-power driven Adruino Lilypad as a controller and a XBee interface as transceiver for each sensor node in order to provide a reliable data collection mechanism with a low amount of power consumption. Furthermore, to upload the collected data to a remote server, we adopt a 3G/LTE cellular network for the long range wireless communication. We utilize a PandaBoard as a gateway to connect the WSN and the 3G/LTE network. The designed network is implemented and tested in a lab scenario at university and on a Marex boat along the coast

An Automatic Ship Detection Method Based on Local Gray-Level Gathering Characteristics in SAR Imagery

This paper proposes an automatic ship detection method based on gray-level gathering characteristics of synthetic aperture radar (SAR) imagery. The method does not require any prior knowledge about ships and background observation. It uses a novel local gray-level gathering degree (LGGD) to characterize the spatial intensity distribution of SAR image, and then an adaptive-like LGGD thresholding and filtering scheme to detect ship targets. Experiments on real SAR images with varying sea clutter backgrounds and multiple targets situation have been conducted. The performance analysis confirms that the proposed method works well in various circumstances with high detection rate, fast detection speed and perfect shape preservation

CRISPR/Cas9-mediated gene manipulation to create single-amino-acid-substituted and floxed mice with a cloning-free method.

Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technology is a powerful tool to manipulate the genome with extraordinary simplicity and speed. To generate genetically modified animals, CRISPR/Cas9-mediated genome editing is typically accomplished by microinjection of a mixture of Cas9 DNA/mRNA and single-guide RNA (sgRNA) into zygotes. However, sgRNAs used for this approach require manipulation via molecular cloning as well as in vitro transcription. Beyond these complexities, most mutants obtained with this traditional approach are genetically mosaic, yielding several types of cells with different genetic mutations. Recently, a growing body of studies has utilized commercially available Cas9 protein together with sgRNA and a targeting construct to introduce desired mutations. Here, we report a cloning-free method to target the mouse genome by pronuclear injection of a commercial Cas9 protein:crRNA:tracrRNA:single-strand oligodeoxynucleotide (ssODN) complex into mouse zygotes. As illustration of this method, we report the successful generation of global gene-knockout, single-amino-acid-substituted, as well as floxed mice that can be used for conditional gene-targeting. These models were produced with high efficiency to generate non-mosaic mutant mice with a high germline transmission rate

In Situ Thermal Decomposition of Exfoliated Two-Dimensional Black Phosphorus

With a semiconducting band gap and high charge carrier mobility, two-dimensional (2D) black phosphorus (BP), often referred to as phosphorene, holds significant promise for next generation electronics and optoelectronics. However, as a 2D material, it possesses a higher surface area to volume ratio than bulk BP, suggesting that its chemical and thermal stability will be modified. Herein, an atomic-scale microscopic and spectroscopic study is performed to characterize the thermal degradation of mechanically exfoliated 2D BP. From in situ scanning/transmission electron microscopy, decomposition of 2D BP is observed to occur at ~400 {\deg}C in vacuum, in contrast to the 550 {\deg}C bulk BP sublimation temperature. This decomposition initiates via eye-shaped cracks along the [001] direction and then continues until only a thin, amorphous red phosphorous like skeleton remains. In situ electron energy loss spectroscopy, energy-dispersive X-ray spectroscopy, and energy-loss near-edge structure changes provide quantitative insight into this chemical transformation process.Comment: In press: 4 figures in main manuscript, 27 pages with supporting informatio

The Value of CT Enhancement Degree in Prognosis of Pancreatic Cancer

Objective: To explore the prognostic value of CT enhancement degree of pancreatic cancer in pancreatic cancer. Methods: From January 2019 to January 2022, 50 patients with pancreatic cancer who came to our hospital for pathological confirmation were selected. Prior to surgery, use multiphase CT of the pancreas to complete enhanced scans. After surgery, the patient's survival period, clinical treatment effectiveness, and imaging data are used as research variables. In the scanning diagnosis, it is necessary to collect the patient's age, gender, tumor location and size, differentiation process, CT value, etc. Create a mathematical model based on the collected data and complete the experimental research work. Results: Univariate analysis showed that the prognosis of pancreatic cancer patients with higher enhancement degree in each stage (P<0.05 in each stage) was better. Multivariate analysis showed that tumor differentiation (P=0.0118), TNM staging (P=0.004), and portal vein enhancement (P<0.001) can be independent predictors of patient prognosis. Conclusion: The lower the CT enhancement degree of pancreatic cancer, the worse the prognosis