Population Genetic Diversity and Clustering Analysis for Chinese Dongxiang Group With 30 Autosomal InDel Loci Simultaneously Analyzed

In comparison with the most preferred genetic marker utilized in forensic science (STR), insertion/deletion analysis possesses further benefits, like absence of stutter peak, low mutation rate, and enabling mixed stain analysis. At present, a total of 169 unrelated healthy Dongxiang individuals dwelling in Dongxiang Autonomous county of Gansu province were recruited in our study to appraise the forensic usefulness of the panel including 30 autosomal diallelic genetic markers. The insertion allele frequencies were in the range of 0.1598 at HLD 111 to 0.8550 at HLD 118. The cumulative match of probability and the combined probability of exclusion were estimated based on independence of pairwise loci, with the values of 3.96 × 10-11 and 0.9886, respectively, which showed tremendous potential of this panel to be qualified for forensic personal identification in Chinese Dongxiang group. And it could also be used as a complementary tool for forensic parentage testing when combined with standard STR genetic markers. Furthermore, calculation of the DA distance and Fst values of pairwise populations, phylogenetic reconstruction, multidimensional scaling analysis, structure clustering analysis were also conducted to probe the genetic relationships between Dongxiang group and the other 30 reference populations. Results demonstrated that Dongxiang ethnic group might be genetically closer related with most Chinese populations involved in our study, especially Tibet groups, Xibe group, and several Han populations

Estimating perfluorocarbon emission factors for industrial rare earth metal electrolysis

Rare earth (RE) metals have been widely applied in new materials, leading to their drastic production increase in the last three decades. In the production process featured by the molten-fluoride electrolysis technology, perfluorocarbon (PFC) emissions are significant and therefore deserve full accounting in greenhouse gas (GHG) emission inventories. Yet, in the ‘2006 IPCC Guidelines for National Greenhouse Gas Inventories’, no method currently exists to account for PFC emissions from rare earth metal production. This research aims to determine emission factors for industrial rare earth metals production through on-site monitoring and lab analysis of PFC concentrations in the exhaust gases from rare earth metal electrolysis. Continuous FTIR measurements and time-integrated samples (analysed off-site by high-precision Medusa GC–MS) were conducted over 24–60 h periods from three rare earth companies in China, covering production of multiple rare earth metals/alloys including Pr-Nd, La and Dy-Fe. The study confirmed that PFC emissions are generated during electrolysis, typically in the form of CF4 (∼90% wt of detected PFCs), C2F6 (∼10%) and C3F8 (<1%); trace levels of c-C4F8 and C4F10 were also detected. In general, PFC emission factors vary with rare earth metal produced and from one facility to another, ranging from 26.66 to 109.43 g/t-RE for CF4 emissions, 0.26 to 10.95 g/t-RE for C2F6, and 0.03 to 0.27 g/t-RE for C3F8. Converted to 211.60 to 847.41 kg CO2-e/t-RE for total PFCs, this emissions intensity for rare earths electrolysis is of lower (for most RE production) or similar (Dy-Fe production) level of magnitude to industrial aluminium electrolysis

Forensic characteristics and population genetics of Chinese Kazakh ethnic minority with an efficient STR panel

On the purpose of enhancing the forensic efficiency of CODIS STR loci, new STR loci have been gradually discovered and developed into some commercial multiplex systems. Recently, 22 STR loci including 18 non-CODIS STR loci and four CODIS STR loci were investigated in 501 unrelated healthy individuals of Kazakh ethnic group. Seven to 20 alleles at the different loci were identified and altogether 276 alleles for 22 selected loci were detected with the corresponding allelic frequencies ranging from 0.0010 to 0.3623. No significant deviation was observed from the Hardy–Weinberg equilibrium test for any of the 22 STRs. The value of cumulative power of discrimination in Kazakh group was 1-1.00E−28. Analyses of population differentiations and genetic distances between Kazakh and other Chinese groups presented that the Kazakh group with the Uygur group. These 22 STR loci evenly distributed on 22 different autosomal chromosomes were characterized by high genetic diversities and therefore could be utilized in the forensic cases to further increase the discrimination performance

Methodology and applications of city level CO2 emission accounts in China

China is the world's largest energy consumer and CO2 emitter. Cities contribute 85% of the total CO2 emissions in China and thus are considered as the key areas for implementing policies designed for climate change adaption and CO2 emission mitigation. However, the emission inventory construction of Chinese cities has not been well researched, mainly owing to the lack of systematic statistics and poor data quality. Focusing on this research gap, we developed a set of methods for constructing CO2 emissions inventories for Chinese cities based on energy balance table. The newly constructed emission inventory is compiled in terms of the definition provided by the IPCC territorial emission accounting approach and covers 47 socioeconomic sectors, 17 fossil fuels and 9 primary industry products, which is corresponding with the national and provincial inventory. In the study, we applied the methods to compile CO2 emissions inventories for 24 common Chinese cities and examined uncertainties of the inventories. Understanding the emissions sources in Chinese cities is the basis for many climate policy and goal research in the future

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Experiment and Numerical Simulation on Gas-Liquid Annular Flow through a Cone Sensor

The cone meter has been paid increasing attention in wet gas measurement, due to its distinct advantages. However, the cone sensor, which is an essential primary element of the cone meter, plays a role in the measurement of wet gas flow that is important, but not fully understood. In this article, we investigate the gas-liquid annular flow through a cone sensor by experiment and numerical simulation. Emphasis is put on the influences of pressure recovery characteristics and flow structure, and how they are affected by the cone sensor. The results show that the vortex length is shortened in gas-liquid annular flow, compared with that in single-phase gas flow. The pressure recovery length is closely related with the vortex length, and shorter vortex length leads to shorter pressure recovery length. The gas-liquid distribution suggests that flow around the apex of back-cone is very stable, little liquid is entrained into the vortex, and no liquid appears around the low pressure tapping, which makes a more stable pressure at the apex of cone sensor feasible. This finding highlights the importance of obtaining the low pressure from the back-cone apex, which should be recommended in the multiphase flow measurement. Our results may help to guide the optimization of the cone sensor structure in the wet gas measurement

Hardness Results for Total Rainbow Connection of Graphs

A total-colored path is total rainbow if both its edges and internal vertices have distinct colors. The total rainbow connection number of a connected graph G, denoted by trc(G), is the smallest number of colors that are needed in a total-coloring of G in order to make G total rainbow connected, that is, any two vertices of G are connected by a total rainbow path. In this paper, we study the computational complexity of total rainbow connection of graphs. We show that deciding whether a given total-coloring of a graph G makes it total rainbow connected is NP-Complete. We also prove that given a graph G, deciding whether trc(G) = 3 is NP-Complete

Understanding the dissolution of metakaolin in sodium hydroxide solutions

The dissolution kinetics of metakaolin are examined at room temperature in NaOH solutions at concentrations of (0.1, 5, or 10) mol L −1 and at liquid-to-solid mass ratios ( L / S ) of 50 or 1000. Both the dissolved Si and Al concentrations as well as the morphology and structure of the solid are reported at various times. The dissolution rate is approximately constant for about an hour, (0.0043–0.1030) µmol m −2  s −1 , depending on alkalinity and L / S . Some precipitates are observed within the first ten minutes but do not affect the dissolution rate during that first hour, implying that the precipitates form in the solution and do not block or alter the metakaolin surfaces significantly. The dissolution rate during the first 45 min increases with alkalinity and decreases with L / S . The metakaolin exfoliates at higher NaOH concentrations, exposing more surface area to the solution. The effect of exfoliation increases the apparent dissolution rate and promotes more congruent dissolution. These observations not only improve our understanding of the metakaolin dissolution mechanisms but also provide insights into the reactivity of calcined clays that are becoming increasingly important to the sustainability of the cement and concrete industry